Product Datasheet. DYKDDDDK Epitope Tag Antibody NB Unit Size: 0.1 ml. Store at 4C. Do not freeze. Publications: 21

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1 Product Datasheet DYKDDDDK Epitope Tag Antibody NB Unit Size: 0.1 ml Store at 4C. Do not freeze. Publications: 21 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/20/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB DYKDDDDK Epitope Tag Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer 0.1 ml 1.0 mg/ml Store at 4C. Do not freeze. Polyclonal 0.09% Sodium Azide IgG Immunogen affinity purified PBS Page 1 of 4 v.20.1 Updated 2/20/2019 Product Description Host Species Immunogen Goat Monkey, Non-species specific Goats were immunized with ECS, Enterokinase Cleavage Site (xxxddddk), conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Product Application Details Applications Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation Recommended Dilutions Western Blot 1:1000-1:30000, ELISA 1:100-1:500 (coating); 1:1000-1:30000 (primary), Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1:100-1:400 Application Notes Use in immoprecipitation reported in scientific literature (PMID )

3 Images Western Blot: DYKDDDDK Epitope Tag Antibody [NB ] - Analysis using the Agarose Immobilized conjugate of NB DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag. Page 2 of 4 v.20.1 Updated 2/20/2019 Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB ] - Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h +l). Western Blot: DYKDDDDK Epitope Tag Antibody [NB ] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.04 ug/ml (1:25,000). Publications Yang Y, Qin M, Bao P et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of alpha-synuclein via exosome PLoS ONE 2017 Jul 12 [PMID: ] (WB, Human) Gorur A, Yuan L, Kenny SJ et al. COPII-coated membranes function as transport carriers of intracellular procollagen- 1. biorxiv. Feb :00AM [PMID: ] (ICC/IF, WB, Human) Morii M, Kubota S, Honda T et al. Src Acts as an Effector for Ku70-dependent Suppression of Apoptosis through Phosphorylation of Ku70 at Tyr-530. J. Biol. Chem. Dec :00AM [PMID: ] (WB) Martin FJ, Prince AS. TLR2 regulates gap junction intercellular communication in airway cells J Immunol

4 Date D, Dreier MR, Borton MT et al. Effects of phosphatase and proteasome inhibitors on Borealin phosphorylation and degradation J Biochem Yun HJ, Park J, Ho DH et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25 Exp Mol Med Jul :00AM This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB ). Sebastian S, Grutter C, Strambio de Castillia C et al. An invariant surface patch on the TRIM5alpha PRYSPRY domain is required for retroviral restriction but dispensable for capsid binding J Virol Mallilankaraman K, Cardenas C, Doonan PJ et al. MCUR1 is an essential component of mitochondrial Ca(2+) uptake that regulates cellular metabolism Nat Cell Biol (IP, Primate) This publication used the Agarose Immobilized conjugated form of this antibody (Cat# NB ). Yun HJ, Park J, Ho DH et al. LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25 Exp Mol Med 2013 [PMID: ] This citation used the Agarose Immobilized version of this antibody. Mallilankaraman K, Cardenas C, Doonan PJ et al. MCUR1 is an essential component of mitochondrial Ca(2+) uptake that regulates cellular metabolism Nat Cell Biol 2012 Dec [PMID: ] (IP, Primate) This citation used the Agarose Immobilized version of this antibody. Date D, Dreier MR, Borton MT et al. Effects of phosphatase and proteasome inhibitors on Borealin phosphorylation and degradation J Biochem 2012 Apr [PMID: ] This citation used the HRP version of this antibody. Sebastian S, Grutter C, Strambio de Castillia C et al. An invariant surface patch on the TRIM5alpha PRYSPRY domain is required for retroviral restriction but dispensable for capsid binding J Virol 2009 Apr [PMID: ] This citation used the HRP version of this antibody. More publications at Page 3 of 4 v.20.1 Updated 2/20/2019

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF109 HAF017 NB mg Donkey anti-goat, IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-goat, IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat, IgG Isotype Control Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: