Product Datasheet. HA Tag Antibody NBP Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet HA Tag Antibody NBP Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 9/16/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP HA Tag Antibody Product Information Unit Size Concentration Storage Clonality 0.1 ml Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Polyclonal Preservative 0.025% Proclin 300 Isotype Purity Buffer IgG Immunogen affinity purified PBS (ph 7), 20% Glycerol Page 1 of 4 v.20.1 Updated 9/16/2018 Product Description Host Species Immunogen Rabbit Human The exact sequence is proprietary. Product Application Details Applications Western Blot, ELISA, Flow Cytometry, Immunoblotting, Immunocytochemistry/Immunofluorescence, Immunoprecipitation Recommended Dilutions Western Blot 1:500-1:3000, Flow Cytometry, ELISA 1:1000-1:10000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:10-1:500, Immunoblotting 1:100-1:2000 Application Notes Images Flow usage reported by customer review. Immunoblotting Assay-dependent dilution Western Blot: HA Tag Antibody [NBP ] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of HA-human AKTtransfected 293T cells 7.5 % SDS-PAGE HA tag antibody dilution: 1:5000

3 Immunocytochemistry/Immunofluorescence: HA Tag Antibody [NBP ] - Analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using antibody at 1:500 dilution. Page 2 of 4 v.20.1 Updated 9/16/2018 Flow Cytometry: HA Tag Antibody [NBP ] - Western Blot: HA Tag Antibody [HRP] [NBP ] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (HRP) diluted at 1:3000. Image using the HRP form of this antibody. Flow Cytometry: HA Tag Antibody [NBP ] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Image from verified customer review. ELISA: HA Tag Antibody [NBP ] - An ELISA plate is coated with 50 ul of a DDDDK Tag conjugated BSA at concentration of 5 ug/ml. The peptide conjugated carrier protein is coated and detected with anti- DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/ml.

4 Immunoprecipitation: HA Tag Antibody [NBP ] - Immunoprecipitation of HA tag protein from 293T whole cell extracts using 5 ug of HA tag antibody. Western blot analysis was performed using HA tag antibody EasyBlot anti-rabbit IgG was used as a secondary reagent. Page 3 of 4 v.20.1 Updated 9/16/2018

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NBP HAF008 NB7156 NBP NBP ug Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody Rabbit IgG Isotype Control Recombinant Human HA Tag Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: