Product Data Sheet - TRUEMAB

Transcription

1 Name:TUBAL3 mouse monoclonal antibody,clone 4D8 Product Data Sheet - TRUEMAB Catalog: TA Components: TUBAL3 mouse monoclonal antibody,clone 4D8 ( 1 vial of 20ug myc-ddk tagged TUBAL3 HEK293T over-expression lysate lyophilized in RIPA buffer (LC411058). (Reconsitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids of human TUBAL3(NP_079079) produced in E.coli. Host: Mouse Isotype: IgG2b Species Reactivity: Human Guaranteed Applications: WB, IHC, IF, FC Suggested Dilutions: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, Concentration: 1.00 mg/ml Buffer: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from mouse ascites fluids by affinity chromatography Storage Condition: Shipped at -20C. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Homo sapiens tubulin, alpha-like 3 (TUBAL3), transcript variant 1 Alternative Name: Database Link: FLJ21665; MGC119347; MGC NP_ Function:

2 Validation Data HEK293T cells were transfected with the pcmv6-entry control (Left lane) or pcmv6-entry TUBAL3 (RC216194, Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tubal3. colon tissue using anti-tubal3 mouse monoclonal antibody. Kidney tissue using anti-tubal3 mouse monoclonal antibody. Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-tubal3 mouse monoclonal

3 liver tissue using anti-tubal3 mouse monoclonal antibody. (Heatinduced epitope retrieval by Tris-EDTA, ph8.0, pancreas tissue using anti-tubal3 mouse monoclonal antibody. endometrium tissue using anti-tubal3 mouse monoclonal Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti- TUBAL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, ph8.0, prostate tissue using anti-tubal3 mouse monoclonal antibody.

4 Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-tubal3 mouse monoclonal lymphoma tissue using anti-tubal3 mouse monoclonal tonsil using anti-tubal3 mouse monoclonal antibody. (Heatinduced epitope retrieval by Tris-EDTA, ph8.0, Anti-TUBAL3 mouse monoclonal antibody ( immunofluorescent staining of COS7 cells transiently transfected by pcmv6-entry TUBAL3(RC216194).

5 HEK293T cells transfected with either RC overexpress plasmid(red) or empty vector control plasmid(blue) were immunostained by anti-tubal3 antibody(, and then analyzed by flow cytometry. Flow cytometric Analysis of Hela cells, using anti-tubal3 antibody(,(red), compared to a nonspecific negative control antibody,(blue). Flow cytometric Analysis of Jurkat cells, using anti-tubal3 antibody(,(red), compared to a nonspecific negative control antibody,(blue). * More validation images may be available on our website: