Product Data Sheet - TRUEMAB

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1 Name:KRT7 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) Product Data Sheet - TRUEMAB Catalog: TA Components: KRT7 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5) (TA802932) WB positive control also available: 20ug myc-ddk tagged KRT7 HEK293T over-expression lysate lyophilized in RIPA buffer (LC401707). (Reconstitute into 20ul of 1x SDS sample buffer before loading; load 5ul per lane as WB control or as desired) Amount: 100ul Immunogen: Human recombinant protein fragment corresponding to amino acids and of human KRT7 (NP_005547) produced in E.coli. Host: Mouse Isotype: IgG1 Species Reactivity: Human Guaranteed Applications: WB, IHC, FC Suggested Dilutions: WB 1:500, IHC 1:150, Concentration: 1 mg/ml Buffer: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Purification: Purified from mouse ascites fluids by affinity chromatography Storage Condition: Shipped at -20C or with ice packs. Upon delivery store at -20C. Dilute in PBS (ph7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. Target Target Name: Alternative Name: Database Link: Function: Homo sapiens keratin 7, type II (KRT7) CK7; K2C7; K7; SCL NP_ Entrez Gene 3855 Human The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

2 The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008].

3 Validation Data HEK293T cells were transfected with the pcmv6- ENTRY control (Left lane) or pcmv6-entry KRT7 (RC201124, Right lane) cdna for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- KRT7. Western blot analysis of A549 cell lysate (35ug) by using anti-krt7 monoclonal antibody. Dilution: 1:500 Human Kidney tissue within the normal limits using anti-

4 Human liver tissue within the normal limits using anti- Carcinoma of Human liver tissue using anti-krt7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 120C for 3min, TA802932) Human lung tissue within the normal limits using anti-

5 Carcinoma of Human lung tissue using anti-krt7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, ph6.0, 120C for 3min, TA802932) Flow cytometric Analysis of living OVCAR-3 cells,using anti-krt7 antibody(ta802932),(red),compared to a nonspecific negative control antibody,(blue). * More validation images may be available on our website: