Microplates. Plate colour Clear plates offer the best solution for absorption, Elisa, spectrophotometric and storage applications.

Transcription

1 The right plate for your appliation Choosing the right plate is the first step to a suessful experiment. We offer a wide range of plates to support different appliations and levels of throughput. The following information will help you hose the most suitable plate material, olour, base and shape: Frame Materials Polypropylene has very low biomoleular binding harateristis, a high temperature tolerane and resistane to many standard laboratory hemials, inluding DMSO. It is mainly used for storage appliations. Polystyrene is a hard material with optial larity. It is used for ell ulture, fluoresene, luminesene, ELISA and homogeneous assays. Well shape Round wells are suitable for most appliations as they redue droplet effets and wiking. Square wells ensure the best use of spae and improve sample mixing partiularly when used for baterial ulture growth. Plate olour Clear plates offer the best solution for absorption, Elisa, spetrophotometri and storage appliations. Blak solid base plates are reommended for top reading fluoresene instrumentation beause of their low bakground and minimised light sattering. Blak lear bottom plates are suitable for bottom reading instruments and mirosopy appliations. White plates give the best results for luminesene detetion beause they maximise signal intensity. Well bottoms Flat bottoms are most suitable for optial imaging and ell ulture appliation while U-bottoms are better for washing, mixing and pelleting and give high surfae area. Conial V-bottoms are best for preipitation, entrifugation and small volume reovery. 84

2 384-Deep Well Plates 190µl 121 o AUTO CL AVAB LE VOLUME = 190µl 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. Square well plates make the best use of spae and improve mixing. A small radius on eah orner prevents sample wiking. Our deep-well plates are ompatible with robotis and automation. Baroding is available as a ustomer order. A silione sealing mat to fit 4ti-0147 is also available. a b Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): 4ti ± 0.25 mm ± 0.25 mm ± 0.25 mm 3.46 ± 0.25 mm 8.99 ± 0.25 mm ± 0.25 mm 4.5 ± 0.25 mm Suitable for adhesive and heat sealing Sealing mat available Produed in lean room faility Free from RNase, Dnase and human genomi DNA Baroding (page 54) Code Desription Quantity 4ti ul, 384 square well, v-bottom deep-well plate, polypropylene, lear 100 plates 4ti square well silione sealing mat 50 mats 384 square well sealing mat Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 85

3 Round Bottomed 96well Shallow Well Storage Plates 121 o AUTO CL AVAB LE 4ti-0110 VOLUME = 300µl 4ti-0116 VOLUME = 350µl 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. The round-wells maximise sample retrieval yet minimise wiking (apillary ation). Round wells maximise sample retrieval, but minimise wiking Produed in lean-room failities Suitable for adhesive and heat sealing Compatible with roboti systems Colletion plate from filter system Free from RNase, DNase, Human Genomi DNA a b 4ti ti ± 0.10 mm ± 0.25 mm ± 0.10 mm ± 0.25 mm ± 0.10 mm ± 0.25 mm 9.77 ± 0.10 mm 12.4 ± 0.25 mm Well diameter (): 7.00 ± 0.10 mm 8.5 ± 0.25 mm Distane to entre of A1 from top edge: ± 0.10 mm ± 0.25 mm Distane to entre of A1 from left edge: ± 0.10 mm ± 0.25 mm Pith (distane between A1 and A2): 9.0 mm 9.0 mm Code Desription Quantity 4ti µl 96 round-well, U-bottom plate, polypropylene, lear 100 plates 4ti µl 96 round-well, U-bottom plate, polypropylene, lear 100 plates 4ti round well silione mat (not for use with 4ti-0110) 50 mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. Baroding (page 54) 86

4 V- Bottom 96well Shallow Well Storage Plates 121 o AUTO CL AVAB LE MAX = 330µl 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. The onial v-bottom improves preipitation, entrifugation and small volume reovery. a b Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): 4ti ± 0.25 mm ± 0.25 mm ± 0.25 mm ± 0.25 mm 8.5 ± 0.25 mm ± 0.25 mm ± 0.25 mm 9.0 mm Round wells maximise sample retrieval, but minimise wiking Colletion plate for filter systems Produed in lean room failities Suitable for adhesive and heat sealing Sealing mat available Free from RNase, DNase and human genomi DNA Baroding (page 54) Code Desription Quantity 4ti µl 96 round-well, V-bottom plate, polypropylene, lear 100 plates 4ti round well silione mat 50 mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 96 round well sealing mat 87

5 1,2ml Deep 96well Round-Well Storage Plates 121 o AUTO CL AVAB LE Clear MAX = 1,2ml 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. The round-wells maximise sample retrieval yet minimise wiking (apillary ation) and the plates are ompatible with robotis and automation. b a Well diameter (): Distane to entre of A1 from top edge: ± 0.25 mm ± 0.25 mm ± 0.25 mm 8.3 ± 0.25 mm ± 0.25 mm Distane to entre of A1 from left edge: ± 0.25 mm Pith (distane between A1 and A2): 9.0 mm Cross-ontamination eliminated by himney-style wells Produed in lean room failities Round wells maximise sample retrieval but minimise wiking Suitable for adhesive and heat sealing Sealing mat available RNase, DNase, Human Genomi DNA Free Baroding (page 54) Code Desription Quantity 4ti ml, 96 round-well, U-bottom low profile deep-well plate, polypropylene, lear 50 plates 4ti Well ap mat (for 4ti-0120 only) 100 mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 4ti Well ap mat (for 4ti-0120 only) 88

6 2,0ml Deep 96well Round-Well Storage Plates 121 o AUTO CL AVAB LE Clear MAX = 2,0ml 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. The round-wells maximise sample retrieval yet minimise wiking (apillary ation) and the plates are ompatible with robotis and automation. b a Well diameter (): Distane to entre of A1 from top edge: ± 0.25 mm 45.0 ± 0.25 mm ± 0.25 mm 8.53 ± 0.25 mm ± 0.25 mm Distane to entre of A1 from left edge: ± 0.25 mm Pith (distane between A1 and A2): 9.0 mm Cross-ontamination eliminated by himney-style wells Produed in lean room failities Round wells maximise sample retrieval but minimise wiking Suitable for adhesive and heat sealing Sealing mat available RNase, DNase, Human Genomi DNA Free Baroding (page 54) Code Desription Quantity 4ti ml, 96 Round-well, U-bottom deep-well plate, polypropylene, lear 50 plates 4ti well round well silion mat 50mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 4ti well ap mat 89

7 1.2ml 96Well Square Storage Plates 121 o AUTO CL AVAB LE Clear MAX = 1,2ml 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. Square well plates make the best use of spae and improve mixing. Our deep-well plates are ompatible with robotis and automation. A small orner radius on eah well prevents sample wiking. Square wells improve mixing Produed in lean-room failities Suitable for adhesive and heat sealing Sealing mat available RNase, DNase, Human Genomi DNA Free b a Well diameter (): 4ti ± 0.25 mm ± 0.25 mm ± 0.25 mm 8.34 ± 0.25 mm Baroding (page 54) Distane to entre of A1 from top edge: ± 0.25 mm Distane to entre of A1 from left edge: ± 0.10 mm Pith (distane between A1 and A2): 9.0 mm Code Desription Quantity 4ti ml 96 square-well, U-bottom deep-well plate, polypropylene, lear 100 plates 4ti well square well silione sealing mat 50mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 4ti well square well sealing mat 90

8 2.2ml 96Well Square Storage Plates 121 o AUTO CL AVAB LE Clear MAX = 2,2ml 4titude miroplates are produed in lean room failities whih are ertified free of RNase, DNase, human genomi DNA and endotoxin. We use only the highest medial grade virgin polypropylene with high hemial resistane against hemials suh as DMSO, phenol, and hloroform. Square well plates make the best use of spae and improve mixing. Our deep-well plates are ompatible with robotis and automation. A silione sealing mat to fit 4ti-0136 is also available. Square wells improve mixing Produed in lean-room failities Suitable for adhesive and heat sealing Sealing mat available RNase, DNase, Human Genomi DNA Free A small orner radius on eah well prevents sample wiking. b a 4ti ± 0.25 mm ± 0.25 mm ± 0.10 mm Baroding (page 54) Well diameter (): 8.20 ± 0.10 mm Distane to entre of A1 from top edge: ± 0.25 mm Distane to entre of A1 from left edge: ± 0.10 mm Pith (distane between A1 and A2): 9.0 mm Code Desription Quantity 4ti ml 96 square-well, U-bottom deep-well plate, polypropylene, lear 50 plates 4ti well square well silione sealing mat 50 mats Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 4ti well square well sealing mat 91

9 384-Well Assay Plates Blak, white or lear Sterilisation on request WORKING = 120µl The 4titude 384well polystyrene assay plates have flat bottom wells and have been designed for fluoresene, luminesene and absorbene appliations. The plates give optimum results for most top-reading instruments and onform to standard SBS footprint. Blak plates have a low bakground fluoresene and minimise light sattering, white plates maximise the signal for luminesene measurements. These high quality plates assure the neessary auray and onsisteny for automated high throughput systems. b a Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm ± 0.25 mm ± 0.25 mm 3.70 ± 0.10 mm 8.99 ± 0.25 mm ± 0.25 mm 4.50 ± 0.10 mm Wiking and bubble formation redued by rounded edges Redued bakground fluoresene Optimum signal to noise ratios Alphanumeri grid referene Lids available Suitable for adhesive and heat sealing Free from DNase, RNase and human genomi DNA Baroding (page 54) Code Desription Plates/Case 4ti well Assay Plate, blak, non-sterile 100 plates 4ti well Assay Plate, white, non-sterile 100 plates 4ti well Assay Plate, lear, non-sterile 100 plates 4ti-0280 Assay Plate lid, low profile, (no ondensation rings), non-sterile 60 lids Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 92

10 96-Well Assay Plates Blak or white Sterilisation on request WORKING = 350µl The 4titude 96well polystyrene assay plates have flat bottom wells and have been designed for fluoresene, luminesene and absorbene appliations. The plates give optimum results for most top-reading instruments and onform to standard SBS footprint. Blak plates have a low bakground fluoresene and minimise light sattering, white plates maximise the signal for luminesene measurements. These high quality plates assure the neessary auray and onsisteny for automated high throughput systems. b a Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm ± 0.25 mm 10.8 ± 0.25 mm 6.3 ± 0.10 mm ± 0.25 mm ± 0.25 mm 9.0 mm Chimney well design to overome optial rosstalk and ontamination Redued bakground fluoresene Optimum signal to noise ratios Alphanumeri grid referene Lids with ondensation rings available Suitable for adhesive and heat sealing Free from DNase, RNase and human genomi DNA Baroding (page 54) Code Desription Quantity 4ti well Assay Plate, blak 100 plates 4ti well Assay Plate, white 100 plates 4ti-0282 Assay Plate Lid, low profile, with ondensation rings, non-sterile 80 lids 4ti-0283 Vision Plate 96 Lid, low profile, with ondensation rings, sterile. 80 lids Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 93

11 24-Well Assay Plates Blak Sterilisation on request WORKING = 1880µl The 4titude 24well polystyrene assay plates have flat bottom wells and have been designed for fluoresene, luminesene and absorbene appliations. The plates give optimum results for most top-reading instruments and onform to standard SBS footprint. Blak plates have a low bakground fluoresene and minimise light sattering. This high quality plate assures the neessary auray and onsisteny for automated high throughput systems. b a Plate height: Well depth: Well diameter: Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm 15.0 ± 0.25 mm 12.5 ± 0.25 mm 14.5 ± 0.10 mm ± 0.25 mm ± 0.25 mm 18.0 mm Code Desription Quantity 4ti well Assay Plate, Blak 100 plates 4ti-0284 Assay Plate Lid, Low Profile, with Condensation Rings, Non-Sterile 80 lids 4ti-0286 Assay Plate Lid, Low Profile, with Condensation Rings, sterile 80 lids Chimney well design to overome optial rosstalk and ontamination Redued bakground fluoresene Optimum signal to noise ratios Alphanumeri grid referene Lids with ondensation rings available Suitable for adhesive and heat sealing Free from DNase, RNase and human genomi DNA Baroding (page 54) Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. 94

12 Vision Plates Vision Plates are our best plates for your most advaned appliations. When visualising fluoresent signals from anything between a single moleule to a whole ell, Vision Plates will give aurate and onsistent results. Suitable for ell based high ontent sreening, onfoal mirosopy, FRET and homogeneous assays, results show an optimum signal to noise ratio and a high onsisteny essential for automated high throughput. Using state-of-the-art manufaturing tehnology 4titude have developed a produt whih offers key advantages to the end user. Laser Welding Tehnology 4titude s Vision Plates are assembled using unique patented laser welding tehnology. The use of loalised welding heat dramatially redues base film distortion during prodution. This improves base flatness, whih in turn redues instrument auto-fousing time. Optial Quality of the Polymer Film The lear base omponent of our Vision Plate demonstrates superior properties in terms of optial larity (low absorbane and high transmission), low bakground fluoresene and onsisteny of material thikness. The latest extrusion tehnology is used for manufaturing an ultra lear base of 190µm thik, to provide optimum results with onfoal mirosopy and laser based detetion systems. Variation aross the plate is minimised so the time required for omplex sreening appliations an be redued dramatially. In addition, ell plate types are available with a 700µm moulded base. This feature further improves the plate flatness and is ritial for ertain onfoal readers, suh as the IN Cell Benefits: Optimum signal-to-noise ratios Redued autofluoresene Standard or 700µm polystyrene base options Good ell adhesion Cyto-toxi free Leak free SBS format Baroding options available Wiking and bubble formation eliminated Compatible sealing options Also suitable for homogeneous assays 95

13 Redution of Autofluoresene 4titude s Vision Plates are assembled using unique patented laser welding tehnology whih redues autofluoresene and does not inhibit ell growth. Other manufaturers assemble lear base miroplates by gluing a lear film to the frame or heat-welding the omponents together. Both tehniques an ause problems with ell growth and the subsequent mirosopi or fluorometri analysis. Heat welding of the two plate omponents under high pressure results in autofluoresene at the well edges, alled halo effet. Gluing uses organi solvents within the adhesives, whih an also ause autofluoresene. These solvents an also have ytotoxi effets, whih may lead to inhibition of ell growth or even ell death. Additionally, inomplete glue lines or weld lines often result in well-to-well leakage. Figure 2: Vision Plate 384 showing ripple-free bonding of frame and base Autofluoresene Intensity Fluoresene Intensity (Ex365nm/Em520nm) titude A B Figure 1: Graph to show the intensity of autofluoresene of 4titude s Vision Plate ompared to leading brand ompetitors A, B & C C Figure 3: Competitor s plate showing areas with no adhesive Figure 4: Competitor s plate showing adhesive ingressing into plate wells With optimum signal-to-noise ratios and good ell adherene, Vision Plates are designed for high ontent sreening and are ideal for homogenous assays. 96

14 Improved Cell Adhesion Polymers, suh as polystyrene are very hydrophobi and need to be surfae treated to inrease wettability and to allow for ell adhesion. To introdue the neessary harges to the plasti surfae, sreening plates are ommonly undergoing orona treatment or similar low ost modifiations. The drawbaks of suh treatments are they are partly reversible and the density of the harges varies aross the surfae area, resulting in uneven ell growth and a short shelf-life of the produts. 4titude uses a unique low pressure plasma proess for treating the plasti surfae whih produes a more onsistent and stable surfae. The treatment has been espeially designed to improve ell adhesion under diffiult onditions (e.g. redued serum onditions). It is also useful for growing ells with low adhesion properties suh as transfeted ells overexpressing proteins. 4titude offers oatings with Collagen 1 and Poly D-Lysin. Collagen 1 Collagen 1 is a protein of the extraellular matrix (ECM), an interellular substane whih, in vivo, influenes adhesion, migration and proliferation among other proesses. Collagen 1 is one of the most important ECM proteins for in vitro ell ultures. Diffiult to ultivate ells adhere to Collagen 1 and display positive growth. Collagen 1 an also influene differentiation and morphology. Poly-D-Lysin Poly-D-Lysin is a syntheti moleule that improves the adhesion of different ell types to polystyrene surfaes. It an improve ultivation effiieny of individual ell lines, espeially when serumfree or serum-redued medium is used, or when experiments suh as transfetions are performed. 4titude s Vision Plate Light mirosope image of 3 different ell lines in the 4titude Vision Plate 384 U2-OS (osteosaroma) ells HeLa (ervial epitheloid arinoma) ells HuH (hepatoblastoma) ells Competitior s Sreening Plate Light mirosope image of 3 different ell lines in a ompetitor s 384well sreening plate 2000 ells were seeded in eah well and inubated for 48 hours to ahieve 90% onflueny. They were then fixed in PFA and stained with Hoehst nulear stain, followed by 5 wash steps in a PW384. U2-OS ells stayed adherent in both plates, but the more sensitive HeLa and HuH ells only stayed adherent in all areas of the 4titude Vision Plate. Figures 5: Comparison showing the ell adherene of the 4titude Vision Plate (top) versus a ompetitor s (bottom) 97

15 Vision Plates NEW UltraVision Plates in 384well and 96well format Blak (384well), lear (96well) WORKING = 120µl 4ti-0214 WORKING = 350µl 4ti-0234 UltraVision plates onsist of a frame and a speial ultralear base with improved transmision at low wavelengths (see graph below). The ultralear base allows DNA measurements at 260/280nm wavelengths in a medium or high throughput. The plates fit most miroplate readers and an be handled easily by roboti systems. Ultralear base improves transmission for low wavelengths Peel-bak film on the base for srath free surfae (384well plate) Optimum signal-to-noise ratios 4ti-0214 Alphanumeri grid referene Low profile lid available b a Well diameter (): ± 0.25 mm ± 0.25 mm ± 0.25 mm 3.70 ± 0.10 mm Suitable for adhesive and heat sealing Free from DNase, RNase and human genomi DNA Distane to entre of A1 from top edge: 8.99 ± 0.25 mm Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm 4.50 mm Code Desription Quantity 4ti-0214 UltraVision Plate 384, non-sterile, UV base 30 plates 4ti-0234 UltraVision Plate TM 96, non-sterile, UV base 30 plates 4ti well Assay Plate lid, low profile 100 lids 4ti well Assay Plate lid, low profile 100 lids Baroding (page 54) Lihttransmission (gemessen an 2mm Obh Platten) UV Film Standard Film Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. Transmission Wavelength 98

16 Vision Plate 384 Vision Plates The Vision Plate assures the neessary auray and onsisteny for automated high throughput systems, generating optimum signalto-noise ratios. Vision Plates are assembled using unique patented laser welding tehnology that redues autofluoresene and does not inhibit ell growth. Available with standard 190µm or 700µm lear base, whih is suitable for use with IN Cell 3000 analysers. b a Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm ± 0.25 mm ± 0.25 mm 3.70 ± 0.10 mm 8.99 ± 0.25 mm ± 0.25 mm 4.50 mm Code Desription Plates/Case 4ti-0201* Vision Plate 384, sterile, TC treated 24 plates with lids 4ti-0202* Vision Plate 384, sterile, TC treated 30 plates 4ti-0203* Vision Plate 384, sterile 30 plates 4ti-0204* Vision Plate 384, non-sterile, untreated 30 plates 4ti-0205 Vision Plate 384, Collagen 1 treated, sterile 100 plates 4ti-0206 Vision Plate 384, Poly-D-Lysin treated, sterile 100 plates 4ti-0280 Vision Plate lid, low profile, no ondensation rings, non-sterile 100 lids 190µm or 700µm lear base Treated options: Poly D Lysin, Collagen 1 or TC Good ell adherene Alphanumeri grid referene Optimum signal-to-noise ratios Low profile lid available Suitable for adhesive and heat sealing RNase, DNase, Human Genomi DNA Free Baroding (page 54) *700µm ultra-lear base ordering ode Add /700 to the ode to order 700µm base version. e.g. 4ti-0201/700 Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. For instrument ompatibility See our website

17 Vision Plates Vision Plate The Vision Plate assures the neessary auray and onsisteny for automated high throughput systems, generating optimum signalto-noise ratios. Vision Plates are assembled using unique patented laser welding tehnology that redues autofluoresene and does not inhibit ell growth. Available with standard 190µm or 700µm lear base, whih is suitable for use with IN Cell 3000 analysers. b a Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): ± 0.25 mm ± 0.25 mm 10.8 ± 0.25 mm 6.3 ± 0.10 mm ± 0.25 mm ± 0.25 mm 9.0 mm Code Desription Plates/Case 4ti-0221* Vision Plate 96, sterile, TC treated 24 plates with lids 4ti-0222* Vision Plate 96, sterile, TC treated 30 plates 4ti-0223* Vision Plate 96, sterile 30 plates 4ti-0224* Vision Plate 96, non-sterile, untreated 30 plates 4ti-0225 Vision Plate 96, Collagen 1 treated, sterile 24 plates with lids 4ti-0226 Vision Plate 96, Poly-D-Lysin treated, sterile 24 plates with lids 4ti-0282 Vision Plate lid, low profile, 96 ondensation rings, non-sterile 45 lids 190µm or 700µm lear base Treated options: Poly D Lysin, Collagen 1 or TC Good ell adherene Alphanumeri grid referene Optimum signal-to-noise ratios Low profile lid available Suitable for adhesive and heat sealing RNase, DNase, Human Genomi DNA Free Baroding (page 54) *700µm ultra-lear base ordering ode Add /700 to the ode to order 700µm base version. e.g. 4ti-0221/700 Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. For instrument ompatibility See our website

18 Vision Plate 24 Vision Plates The Vision Plate assures the neessary auray and onsisteny for automated high throughput systems, generating optimum signalto-noise ratios. Vision Plates are assembled using unique patented laser welding tehnology that redues autofluoresene and does not inhibit ell growth. Available with standard 190µm or 700µm lear base, whih is suitable for use with IN Cell 3000 analysers. Code Desription Plates/Case 4ti-0241* Vision Plate 24, sterile, TC treated 24 plates with lids 4ti-0242* Vision Plate 24, sterile, TC treated 30 plates 4ti-0243* Vision Plate 24, sterile 30 plates 4ti-0244* Vision Plate 24, non-sterile, untreated 30 plates 4ti-0245 Vision Plate 24, Collagen 1 treated, sterile 24 plates with lids 4ti-0246 Vision Plate 24, Poly D-Lysin treated, sterile 24 plates with lids 4ti-0282 b a Well diameter (): Distane to entre of A1 from top edge: Distane to entre of A1 from left edge: Pith (distane between A1 and A2): Vision Plate lid, low profile, ± 0.25 mm 15.0 ± 0.25 mm 12.5 ± 0.25 mm 14.5 ± 0.10 mm ± 0.25 mm ± 0.25 mm 18.0 mm 96 ondensation rings, non-sterile 45 lids 190µm or 700µm lear base Treated options: Poly D Lysin, Collagen 1 or TC Good ell adherene Alphanumeri grid referene Optimum signal-to-noise ratios Low profile lid available Suitable for adhesive and heat sealing RNase, DNase, Human Genomi DNA Free Baroding (page 54) *700µm ultra-lear base ordering ode Add /700 to the ode to order 700µm base version. e.g. 4ti-0221/700 Barode ordering odes Please add to ordering ode /SBC for Standard Single Baroding or /DBC for Standard Double Baroding. For instrument ompatibility See our website

19 Mimetix Multi-Well Plate 3D ell ulture in a saffold of polymer fibres 102

20 Mimetix TM saffolds for 3D ell ulture: Mimetix TM saffolds are highly porous and mimi the extraellular matrix, providing an ideal environment to support the growth of ells in 3D. They are reated by eletrospinning the medial grade polymer poly-l-latide (PLLA) into fibres. The Mimetix TM saffold is highly onsistent well-to-well with respet to fibre diameter and pore size, resulting in exellent reproduibility of ell-based assays. Mimetix TM has been validated with a number of primary ells, ell lines and stem ells and is available in a range of ready to use, sterile laboratory onsumable formats. Mimetix TM saffold resembles the extraellular matrix. Why Use 3D Models in Drug Disovery? Cells grown in a 3D miro-environment look and behave more like ells in human tissues than those ultured in 2D. They are in ontat with other ells and produe extraellular matrix, whih influenes tissue speifi gene expression, ell growth, and the uptake and metabolism of drugs. 3D ell-based in vitro assays an provide more realisti data on the effiay of drug andidates against tumour ells or their toxiity, and ould potentially redue the risk of failure in expensive linial trials. Mimetix TM multiwell plate The 96 well plate is based on the suessful Visionplate range with superior optial larity of the base and laser welding tehnology to weld bases to the frame with minimal base distortion and no use of ytotoxi glues. It ontains the Mimetix TM saffold laser-welded into the base, and is designed to be ompatible with industry standard for automated handling and imaging equipment. It is available in fibre diameters of 2 µm and 4 µm, orresponding to pore sizes of μm and μm and at a saffold thikness of 50 μm. It is supplied gamma-irradiated in individually sealed plasti wrapping. Mimetix TM benefits in 3D assays True 3D environment high bath-to-bath onsisteny for reproduible ell-based assays Ready to use, sterile, standard size plates are ompatible with industry-standard automated handling and imaging equipment Minimal protool adaption required to swith from 2D to 3D Material does not degrade or alter over the ourse of an experiment Thin saffold provides benefits of 3D ell morphology and behaviour yet allows fluoresent mirosopi imaging Code Desription Plates/Case 4ti-0250/2 96well plate with fibres of 2 μm diameter 24 4ti-0250/4 96well plate with fibres of 4 μm diameter sterile, lidded 24 MCF7 breast aner ells growing within Mimetix TM saffold