Course Syllabus (Academic Year 2018)

Transcription

1 Course Syllabus (Academic Year 2018) School of Interdisciplinary Studies, Kanchanaburi Campus, Mahidol University 1. Course No. and Title : KACB 304 Basic Cellular and Molecular Biology Credit (study hours) : 4 (3-3-7) 2. Program Name : Bachelor of Science in Conservation biology program 3. Course Module : Gen.Edu. course B.Sc. core course CB core course Elective course Pre/co-requisite : BI124, BI125 and KACB Class Semester : 1 st Semester 2 nd Semester Academic Year Class Schedule & Venue : Tuesday, 09:00 12:00, Room 2313, Lecture building, ; Room L-201/L301, Laboratory building 6. Class Coordinator : Lect. Supatra Chunchob Contact No. : supatra.chn@mahidol.edu, supatra191@yahoo.com 7. Course Description Principles of the structure, function, of organelles, physiology of the cell as well as concept of molecular biology of the cell, characteristic and function of biomolecule, protein synthesis, mutation, gene and genome, mechanism of gene expression, cell cycle and regulation, molecular biology and immune systems, genetic engineering, molecular techniques, microtechniques and application for conservation biology. 8. Course Objectives / Course Learning Outcomes (CLOs) No. Objectives / CLOs Expected Skills / Knowledge Specific Generic Knowledge 8.1 To describe important structure and S5 G1 Scientific literacy function of organelles skill To describe important characteristics of G2 Numerical skill S5 each biomolecule and its function in G3 Information literacy 1 skill biological processes G4 Communication 8.3 To describe relationship between Skills organelles, biological process and cell S6, S8 G8 Personal 8.4 response To do the laboratory practices in S16 responsibility G10 Teaming and 4 PLOs

2 molecular biology including DNA extraction gene cloning genetic engineering and genome analysis 8.5 To identify the application of suitable molecular techniques for conservation biology collaboration skill S15, S16 4 NOTE: *PLOs = Program Learning Outcomes PLO 1: Apply skills and knowledge of fundamental and biological sciences for explaining biodiversity. PLO 2: Evaluate functions, value, status, trend, and threats to address biodiversity problems. PLO 4: Evaluate functions, value, status, trend, and threats to address biodiversity problems. S = Specific skills S5 Explain mechanism of genetic variations in organisms. S6 Explain interactions between species, population, community and its environments. S8 Explain functions and value of biodiversity S15 Identify stakeholders and constraints relate to biodiversity conservation S16 Compare techniques, research methodology, and practices appropriate to biodiversity conservation 9. Class Instructor List Name Contact no Supatra Chunchob () supatra.chu@mahidol.ac.th, supatra191@yahoo.com 9.2 Chetsada Phaenark (CP) jetsada2004@hotmail.com,chetsada.pha@mahidol.ac.th 9.3 Special guest 10. Course Outline Lecture Week Date Contents CLOs 1 15 Jan 2019 Course orientation Biomolecules and their function in living cells 2 22 Jan 2019 Basic techniques in molecular biology Overview of organelles and their functions 3 29 Jan 2019 Principle of cytology CP 4 5 Feb 2019 Basic techniques in cytology, 5 CP 5 12 Feb 2019 Mitochondria chloroplast and cell metabolism 2, 3 *6 19 Feb 2019 No class: Makha Bucha Day Nucleus 23 Feb 2019 Class makeup: Nucleus 2, Feb 2019 Endoplasmic reticulum Golgi Lysosome and Protein 2, 3 transportation 8 5 Mar 2019 Cell wall, cell membrane and molecular transportation 2, 3

3 Week Date Contents CLOs 9 Midterm Examination (11 15 Mar 2019) Mar 2019 Cytoskeleton and Cell movement 2, Mar 2019 Cell signaling and regulation 2, Apr 2019 Cell cycle and Program cell death 2, 3 Biology of cancer cell 13 9 Apr 2019 Proteomics and Application Apr 2019 Bioinformatics and Application 5 *Special guest Apr 2019 Field Trip 5, CP 16 7 May 2019 Application of Molecular Biology for Conservation Biology 5 17 Final Examination (13 24 May 2019) Note Lecturer: = Supatra Chunchob, CP = Chetsada Phaenark, KI = Kuntida Ittiporn Laboratory Week Date Contents CLOs Scientist and lab assistant 1 15 Jan 2019 Biosafety and ethics in Molecular biology 4 KI General laboratory equipment 2 22 Jan 2019 Sample collection and stock solution preparation 4 KI 3 29 Jan 2019 Study of cell structure using light microscope 4 CP KI 4 5 Feb 2019 Cell Staining Techniques 4 CP KI 5 12 Feb 2019 Animal Genomic DNA extraction 4 KI 6 19 Feb 2019 Plant Genomic DNA extraction 4 KI 7 26 Feb 2019 Plasmid DNA extraction 4 KI 8 5 Mar 2019 Restriction enzyme analysis and Agarose gel electrophoresis 9 Midterm Examination (11 15 Mar 2019) 4 KI Mar 2019 Polymerase Chain Reaction (PCR) 4 KI Mar 2019 Recombinant plasmid DNA construction and 4 KI Bacteria cell transformation 12 2 Apr 2019 Selection of recombinant bacteria cell 4 KI

4 Week Date Contents CLOs 13 9 Apr 2019 Protein extraction 4 KI Apr 2019 Protein analysis by SDS-PAGE 5 KI Apr 2019 Field trip 4, CP KI 16 7 May 2019 Lab discussion and Conclusion 4 KI Scientist and lab assistant ** Final exam (Laboratory) 4, CP KI 17 Final Examination (13 24 May 2019) Note Lecturer: = Supatra Chunchob, CP = Chetsada Phaenark, KI = Kuntida Ittiporn ** Final exam (Laboratory) : After Hours Study 11. Course Assessment No. Methods / Activities Regulations CLOs Week Mid-term exam (Lecture) Final exam (Lecture) 3 hours exam (other regulations will be announced in the class later) 3 hours exam (other regulations will be announced in the class later) Weight Distribution (%) Final exam 1 hour practical exam 4 (Laboratory) Quiz Every week Field trip Report For Field trip Lab Reports Every week Class participation On time class Total 100

5 12. Grading System Criterion-referenced evaluation Grade Score Grade Score Grade Score Grade Score A 80 % B % C % D % B % C % D % F < 50 % Norm-referenced evaluation *If use both criterion and norm-referenced evaluation, please tick two boxes. 13. References Alberts Bray et al., Essential Cell Biology. 3th edition. Garland Science Cooper, G.M. and Hausman, R.F The Cell: A Molecular Approach. 4th edition. Sinauer Associates, Inc Sunderland, Massachusettes.