NAME OF DEPARTMENT BIOTECHNOLOGY GOVT. V.Y.T.PG. AUTONOMOUS COLLEGE, DURG.

Transcription

1 NAME OF DEPARTMENT BIOTECHNOLOGY GOVT. V.Y.T.PG. AUTONOMOUS COLLEGE, DURG. Approved syllabus for B.Sc. Biotechnology by the members of Board of Studies for Session The proposed syllabus with the paper combinations is as under B.Sc. I Paper I: Biochemistry, Maths and Computers Paper III: Lab Course/ Practical Title: B.Sc. II Paper I: Molecular Biology and Biophysics Paper III: Lab Course/ Practical Title: B.Sc. III Paper I: General Biotechnology Paper III: Lab Course/ Practical Title: Paper II: Cell Biology and Microbiology Paper II: Recombinant DNA Technology Paper II: Immunology The syllabus of B.Sc. Biotechnology is hereby approved for the session

2 Paper No. Syllabus and Marking Scheme for FIRST YEAR Title of the Paper B.Sc. Biotechnology Marks Allotted in Theory Max. Min. I Biochemistry, Maths and Computers 33/50/75 11/17/25 II Cell Biology and Microbiology 33/50/75 11/17/25 III 34/50 11/17 IV Lab Course/Practical /03 Theory Paper - 100/ Practical - 50/100 Total Marks Note: This page should be used as cover page for each year separately

3 BIOTECHNOLOGY B.Sc. Part -I PAPER I BIOCHEMISTRY, MATHS AND COMPUTERS UNIT 1 1. Biochemistry : Introduction, Scope, Development, Definition, Aims and Nature. 2. Carbohydrates : Structure, Classification, and function of mono, oligo and polysaccharide. 3. Proteins : Introduction, Structure, Classification; physical and chemical properties. 4. Amino Acids : Classification, Essential & Non essential, General properties. UNIT Lipids : Structure, Classification and chemical properties. 2. Enzymes : Introduction, Definition, Classification, Co-enzymes and Co-factors, Nomenclature, Mechanism of Enzyme Action, Factors affecting enzyme activity. 3. Hormones : Introduction, Definition, Classification, Structure, Function and Application of plant hormone Auxin and Gibberellins, Animal hormones Pancreas and Thyroid. UNIT 3 1. Carbohydrate metabolism Glycogenesis, Glyconeogenesis, Glycogenolysis, Glycolysis, Krebs cycle. 2. Fat metabolism Introduction, metabolism of glycerol, fatty acid oxidation, Conversion of fat into carbohydrates. 3. Protein metabolism Introduction, conversion of amino acids, decarboxylation, Deamination of amino acids, formation of urea. 4. Biological oxidation oxidation and reduction constituents of electron transport chain mechanism of electron transport in ETC. 5. Enzyme technology introduction, comparison enzyme and catalyst, production, Chemical energetic, enzyme kinetics, immobilization and application, enzyme reactor, biosensor, enzyme engineering. UNIT 4 1. Set theory and its properties, Linear equation. 2. The binomial theorem, Logarithm. 3. Simple Differentiation and Integration. 4. Probability, Calculation, Methods of Sampling. 5. Measurement of Central Tendencies and Deviation. UNIT 5 1. Computers General introduction, Organization of computer, Digital and Analogue.

4 2. Computer algorithm. 3. Computer in online monitoring and automation. 4. Application of computer in co-ordination of solute concentration, ph and temperature of a fermenter in operation. List of Books 1. Nelson and Cox Principles of Biochemistry. 2. Albert L. Lehninger Biochemistry. 3. Todd and Howards Mason Biochemistry. 4. Lubert Stryer and Berg Biochemistry. 5. Diana Rain, Marni Ayers, Barby Textbook of Q level Programming. 6. E.Balaguruswamy Programming in BASIC. 7. Karl Schwartz Guide of Microsoft, Marina Road. DIRECTIVES FOR STUDENTS, FACULTY AND EXAMINERS 1. There shall be three sections (Sections A, B and C) in each theory paper. Section A shall contain very short answer type questions (one or two line answer) or objective type questions (fill in the blank). (No multiple choice questions). Altogether 10 questions will be set from the entire syllabus, and shall be compulsory. (01X10 = 10). 2. Section B shall contain short answer type questions with the maximum limit of 250 words. Altogether 10 questions to be set i.e. two from each unit with the internal choice. The candidates are required to solve one from each unit. (3X5=15). 3. Section C shall contain long answer/ descriptive type questions. The students are required to answer specifically and the answer should not exceed the maximum limit of 450 words. Altogether 10 questions to be set i.e. two from each unit with the internal choice. The candidates are required to solve one from each unit. (5X5=25)

5 UNIT 1 PAPER II CELL BIOLOGY, GENETICS AND MICROBIOLOGY 1. Cell theory and the cell : Idea of cell theory, Shape and Size. 2. Cell wall and Plasma membrane. 3. The nucleus Significance, Structure and Nucleolus. 4. Chromosome Morphology, Chemical Composition, Ultra structure and Special type of chromosome. 5. Mitochondria - Morphology, Chemical Composition, Ultra structure, Origin and Function. 6. Plastids Chloroplasts, ultra structure and function. UNIT 2 1. Cytoskeleton Microtubules : Structure, Chemical Composition, Microtubules in cilia and flagella, role in cell division, Microfilaments in muscle cells and non muscle cells, Muscle contraction. 2. Cytoplasm Structure and Functions of ER, Ribosomes. 3. Golgi complex, Lysosome, Centrosome. 4. Cell division Amitosis, Mitosis, Meiosis, Comparison : Mitosis and Meiosis. 5. Mendel s law of inheritance. 6. Linkage and Crossing over. UNIT 3 1. Structural changes in chromosome : Deletion, Duplication, Translocation, Inversion. 2. Numerical changes in chromosome : Aneuploidy and Euploidy and its importance. 3. Mutation History, Physical and Chemical Mutagens, Detection of mutation in Drosophila and Plants. 4. Human Genetics 5. Structure and synthesis of Nucleic acids. UNIT 4 1. Microbiology Introduction and History. 2. Bacteria Size, Shape and Structure. 3. Classification Bergey s manual. 4. Microbial growth and Nutrition. 5. Reproduction Conjugation, Transformation and Transduction. 6. Genetics of Bacteria, Plasmids, Transposons and Retroposons. UNIT 5 1. Viruses Basic features, Structure, Classification, Multiplication, Bacteriophages : Morphology, Life cycle, Infection and Medicinal importance.

6 2. Mycoplasma History, Classification, Structure, Infection and Disease. 3. Food and Dairy Microbiology Food production, Spoilage and Preservation. 4. Soil Microbiology Soil and Micro-organisms, Biogeochemical cycles (Carbon Sulphur, Nitrogen and Phosphorus cycle). List of Books 1. C.B. Powar Cell Biology, Himalaya Publishing House. 2. Gerald Karp Cell & Molecular Biology. 3. Lewis J. Klein, Smith & Valerie, M. Kish Principles of Cell and Molecular Biology. 4. P.K. Gupta Cell and Molecular Biology. 5. Tortora, Funke and Case Microbiology An Introduction. 6. Prescott, Harley and Klein Microbiology. 7. P. Chakraborthy Textbook of Microbiology. 8. S.S. Purohit Microbiology, Fundamentals and Applications. 9. R.C. Dubey and D.K. Maheshwari Microbiology. 10. R.C. Dubey and D.K. Maheshwari Practical Microbiology. 11. B.R. Vashishtha, A.K. Sinha and V.P. Singh Botany for Degree Students, Part I, S. Chand. 12. C.J. Alexopoulus Introductory Mycology. DIRECTIVES FOR STUDENTS, FACULTY AND EXAMINERS 1. There shall be three sections (Sections A, B and C) in each theory paper. Section A shall contain very short answer type questions (one or two line answer) or objective type questions (fill in the blank). (No multiple choice questions). Altogether 10 questions will be set from the entire syllabus, and shall be compulsory. (01X10 = 10). 2. Section B shall contain short answer type questions with the maximum limit of 250 words. Altogether 10 questions to be set i.e. two from each unit with the internal choice. The candidates are required to solve one from each unit. (3X5=15). 3. Section C shall contain long answer/ descriptive type questions. The students are required to answer specifically and the answer should not exceed the maximum limit of 450 words. Altogether 10 questions to be set i.e. two from each unit with the internal choice. The candidates are required to solve one from each unit. (5X5=25)

7 PRACTICAL LIST EXPERIMENTS 1. Laboratory rules, tools, equipment and other requirements in Microbiological laboratory. 2. Micrometry Use of ocular and stage micrometer. 3. Counting of bacteria by counting chamber, by plate count. 4. Microscopic examination of living micro organisms (a) Temporary wet mount (b) Hanging drop technique. 5. Smears and staining methods (a) Preparation of bacterial smear (b) Simple staining of bacteria (c) Acid fast staining (d) Negative and positive gram staining. 6. Preparation of media and cultivation techniques (a) Basic liquid media (broth) (b) Basic solid media (agar slants and deep tubes) (c) Demonstration of selective and differential media (d) Isolation and enumeration of micro organisms (e) Isolation from air (f) Isolation from soil. 7. Methods of obtaining pure cultures (a) Streak plate method (b) Pour plate method (c) Spread plate method (d) Broth cultures. 8. Growth and Biochemical techniques (a) Determination of bacterial growth (b) Amylase production test (c) Cellulase production test (d) Estimation of sugar in given solution (e) Extraction and separation of lipids (f) Estimation of proteins (g) Isolation and purification of protein (h) Kinetic studies on enzymes (i) Mitosis and Meiosis (j) Biostatistics : By manual and by computer 1. Problems on chi-square text 2. Problems on mean, mode and median.

8 SCHEME OF PRACTICAL EXAMINATION Time : 4 hrs. M.M. : Instrument based experiment (two) Experiment based on culture of micro-organisms Bacterial growth Biochemical techniques Biostatistics Viva voce Record / Sessional Total 50