Departments of Biotechnology. Academic planner for (ODD semesters) Month I Semester III Semester V Semester

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1 Departments of Biotechnology Academic planner for (ODD semesters) Subject: Biotechnology Month I Semester III Semester V Semester JULY AUGUST Cell as a Basic unit of Living Systems, Discovery of cell, The Cell theory. Ultrastructure of an eukaryotic cell (both plant and animal cell). Structure of plasma membrane- Fluid mosaic model and functions of plasma membrane. Chromosomes:Number and Size. Morphology and structural organization, Ultra structure. Special type of chromosome: Structure of DNA and RNA a brief account, Mendelism. Structural organization and function of plasma membrane and cell wall of eukaryotes. Cell division, Cell senescence and death. Interaction of genes. Mutations, Human genetics. Biochemistry: Introduction for Biomolecules. Amino acids: Introduction, General formula. Enzymes:Introduction,classification,enzyme kinetics. Scope and development of Biophysics, ph and buffer concepts. Chemical bonding. Analytical techniques: a)chromatography-paper,thin layer, Column and GLC. b) RPM and Ultracentrifugation. Aminoacid(contd) Classification and properties. Proteins- Introduction and classification. Enzyme (contd) Carbohydrates: Structures, properties and classification, carbohydrates as a source of energy. Vitamins. Spectroscopic techniques.. Introduction to Genetic Engineering. Tools for genetic engineering: Enzymes.(4hrs) Scope of Animal Tissue Culture.. Culture Media and Simulating natural conditions for growth of animal cells. Primary Culture and Secondary Culture. History and scope Immunology. Types of Immunity, Cell and organs of immune responses and their functions. Tools for Genetic engineering (Contd). In Vitro construction of recombinant DNA molecules. Isolation of passenger & vector DNA, creation of rdna.. Transformation of rdna molecules into target host organism. Screening and selection of recombinant host cells. Expression of cloned DNA in E.coli. Treatment of municipal waste and industrial effluents.

2 SEPT Cellular organelles. Sex determination in plants and animals. Linkage and Crossing over. Chromosomal variations. Cytoplasm inheritance Structural organization of proteins- primary, secondary, tertiary and quaternary. Lipids: structure & properties. Hormones: steroid hormones-structure and importance. X-ray crystallography, NMR, IR, Fluorescence and Atomic absorption, Isotopes-Measure of radioactivity, GM counters, Scintillation counter. Transfection of animal cell lines, Expression of Cloned proteins in animal cell. Production of Vaccines in animal Cells. Production and Applications of monoclonal antibodies. Growth factors. Humoral and cell mediated immunity. Antigens and Factors influencing antigenicity. Antibodies, Production of antibodies. Molecular biology techniques. Application of Genetic engineering in human health. Renewable and Non-Renewable resources of energy, Conventional fuels and their environmental impact. Modern fuels and their environmental impact. Bioremediation. Bio fertilizers. Environmental significance of GMO, Plants and animals. Complement system Antigen Antibody reaction, Hypersensitivity and Allergic reactions.

3 Department of Biotechnology Academic Planner for (Even Semesters) Subject: Biotechnology Month II Semester IV Semester VI Semester December 2014 January Part A- Microbiology: Introduction and Scope of Microbiology. Part B- Biostatistics: Importance and Applications- Tabulation and classification of data. Part A- Microbiology: Contributions of Scientists, Branches of Microbiology. Microscopy: Construction and working principles of different Microscopes. Microbial Techniques: A) Sterilization- Physical and Chemical methods. Microbial Taxonomy: Concept of microbial species and Classification of Bacteria. Part B- Biostatistics: Frequency distribution and Graphical distribution of data. Measures of Central tendencies: Mean, Median and Mode and their properties. Molecular Biology Molecular basis of life- An introduction. DNA Replication: Introduction, Central Dogma. Experimental proof of DNA and RNA as genetic material. Nucleic Acids: Watson and Crick model of DNA and other forms of DNA. DNA replication: General mechanism of replication, Enzymes involved in replication, Replication in Prokaryotes and Eukaryotes. DNA Repair: Causes of NDA damage, Mechanism of repair. Structure of Prokaryotic and Eukaryotic Gene: Genetic code, Wobble hypothesis. Introduction to Plant tissue culture. Paper-VIII Industrial Biotechnology Introduction to Industrial Biotechnology and basic principles of Fermentation technology. Role of tissue culture in Agriculture, horticulture and forestry. In-vitro methods of plant tissue culture. Aseptic techniques, Nutrient media and use of growth regulators. Paper-VIII Industrial Biotechnology Screening and Isolation of Microorgamisms, Maintenance of Strain. Fermentation Media and Sterilization techniques. Fermenters, Types of Fermentation.

4 February March April Part A- Microbiology Microbial Techniques: A) Radiation methods. B) Stains and Staining Techniques. General account of Viruses and Bacteria: A) Viruses Eukaryotic Microorganisms: Salient features. Part B- Biostatistics Measures of Dispersion, Hypothesis Testing. Part A- Microbiology General account of Viruses and Bacteria: B) Bacteria. Eukaryotic Microorganisms: Classification and reproduction of fungi, mycoplasma and algae. Pathogenic Microorganisms: A) Bacterial diseases of Man. Microbial Metabolism: A) Respiration. Part B- Biostatistics Probability and Distribution. Part A- Microbiology Pathogenic Microorganisms: B) Viral diseases. Microbial Metabolism: B) Bacterial Photosynthesis. Part B- Biostatistics Models of data presentation. Structure of RNA, Functions of DNA and RNA. Recombination in Prokaryotes: Transformation. Transcription in Prokaryotes and Eukaryotes, Translation in Prokaryotes and Eukaryotes: Mechanism of Translation. Recombination in Prokaryotes: Conjugation and Transduction. Translation in Prokaryotes and Eukaryotes: Post translational modifications of proteins. Regulation of Gene expression in Prokaryotes and Eukaryotes. Insertional elements and Transposons. Gene organization and expression in Mitochnondria and Chloroplast. In-vitro fertilization, Clonal propagation of elite species. Transgenic Plants: Techniques of transformation, Applications of transgenic plants. Edible vaccines from plants. Paper-VIII Industrial Biotechnology Process development and Downstream processing (DSP). Production of Microbial products. Plant cell suspension culture for the production of Food additives. Organ culture: Culture of anther, embryo and endosperm and their applications. Organogenesis and Somatic embryogenesis. Protoplast culture. Biotechnology and Intellectual property rights. Paper-VIII Industrial Biotechnology Enzyme Biotechnology. Fermented Foods. Technique of mass culture of Algae. Microbial Polysaccharides and polyesters. Somaclonal variation and significance. In-vitro production of secondary metabolites.

5 Departments of Biotechnology Academic planner for -16 (ODD semesters) Subject: Biotechnology Month I Semester III Semester V Semester JJUNE & JULY AUGUST Cell as a Basic unit of Living Systems, Discovery of cell, The Cell theory. Ultrastructure of an eukaryotic cell (both plant and animal cell). Structure of plasma membrane- Fluid mosaic model and functions of plasma membrane. Cell wall of eukaryotes. Structure of DNA and RNA a brief account. Mendelism- Mendel and his works. Cellular organelles. Chromosomes: Number and Size. Morphology and structural organization, Ultra structure. Special type of chromosome Interaction of genes. Mutations, Human genetics. Biochemistry: Introduction for Biomolecules. Amino acids: Introduction, General formula. Classification and properties. Proteins-Introduction and classification. Carbohydrates: Structures, properties and classification, carbohydrates as a source of energy. Lipids. Scope and development of Biophysics, Structural organization of proteinsprimary, secondary, tertiary and quaternary. Lipids(contd.), Enzymes: Introduction, classification, enzyme kinetics. Vitamins. ph and buffer concepts. Chemical bonding. Introduction to Genetic Engineering. Tools for genetic engineering: Environmental Biotechnology: Renewable and non renewable sources of energy, Conventional fuels, Modern fuels and their impact. History and scope Immunology. Types of Immunity, Cells and organs of immune responses and their functions. Antigens, Antibodies. In Vitro construction of recombinant DNA molecules. Isolation of passenger & vector DNA, creation of rdna. Transformation of rdna molecules into target host organism. Screening and selection of recombinant host cells. Expression of cloned DNA in E.coli. Bioremediation. Treatment of municipal waste and industrial effluents.

6 Complement system Antigen-Antibody reactions. SEPT Cellular organelles. Cell division, Cell senescence and death. Sex determination in plants and animals. Linkage and Crossing over. Chromosomal variations. Cytoplasm inheritance Hormones: steroid hormones-structure and importance. Analytical techniques: a)chromatography-paper, Thin layer, Column and GLC. b) RPM and Ultracentrifugation. Spectroscopic techniques: X-ray crystallography, NMR, IR, Fluorescence and Atomic absorption, Isotopes-Measure of radioactivity, GM counters, Scintillation counter. Molecular biology techniques. Application of Genetic engineering in human health. Bio fertilizers. Environmental significance of GMO. Hypersensitivity and Allergic reactions. Blood cell components, Vaccines and Immunization. Scope of Animal Tissue Culture. Culture Media and Simulating natural conditions for growth of animal cells. Primary Culture and Secondary Culture. Transfection of animal cell lines, Expression of Cloned proteins in animal cell. Production of Vaccines in animal Cells. Production and Applications of monoclonal antibodies. Growth factors. Trnasgenic animals.

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