Integrated Milk Testing TM Top quality milk through individual bacteria counting BactoScan FC

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1 Integrated Milk Testing TM Top quality milk through individual bacteria counting BactoScan FC FOSS Slangerupgade 69 DK-3400 Hilleroed Denmark Tel.: Fax: P/N , Issue 7GB, November 2011 Dedicated Analytical Solutions

2 Obtain and sustain top quality raw milk Improve on-farm hygiene Bacterial contaminations may go undetected for days, due to the time needed with plate count methods, making trouble shooting more difficult for the farmer. But thanks to the unique BactoScan method, reliable results can now be reported quickly, at the next milk collection, or even on-line to the farmer immediately after testing. This has made the BactoScan method a valuable tool for monitoring on-farm hygienic status, and this is one reason why the BactoScan method is used for payment purposes on more than 90 % of all milk within the European Union. Better payment schemes Methods based on the traditional plate count method require days of incubation, making bacterial counts a bottle neck when reporting payment parameters to dairies and farmers. On top of this, increased attention is paid to the lower repeatability and reproducibility of these methods. The BactoScan method on the other hand is designed to give you instant and reliable results, with high accuracy, repeatability and repeatability, so a full set of parameters can be reported faster than ever. This new generation BactoScan FC which runs up to 150 samples per hour will make life much easier for you, offering unique precision and accuracy which ensures an even more reliable grading. Dairy benefits Serious contaminations can go undetected right through the dairy production when using traditional methods, so dairies use the rapid and reliable BactoScan method to monitor incoming raw milk. This in order to reject it or to channel it to the production for which it is best suited. The BactoScan method is the only worldwide recognised method that gives all the time you need to take appropriate action. The longer durability of high-quality raw milk, which can be sustained by the quick BactoScan response to farmers, gives dairies a direct economic benefit by enabling higher flexibility in collection, transportation and production. Worldwide recognition The BactoScan method has become the industrial standard for counting bacteria in more than 40 countries and has obtained numerous approvals. Hundreds of BactoScan instruments are in use worldwide, and more than 90 % of all milk within the EU is paid for based on BactoScan results. User organisations have seen a drastic improvement in milk quality shortly after introducing BactoScan. This development will be further speeded-up by the introduction of the BactoScan FC.

3 BactoScan FC for automa ted total bacteria count BactoScan FC gives You the benefits of modern technology: Member of the Integrated Milk Testing Family, offering: Easier reagent handling! Every customer has special reasons for choosing our equipment, and we do our best to offer many good reasons. Compared to traditional breeding methods, the cost efficient BactoScan FC will offer you: - Tremendous reductions in the amounts of waste to be disposed of - Simplified laboratory procedures, no Petri dishes, no agar, no heating/incubation units - Large reductions in manpower per sample, and in addition less operator dependency - A network of competent factory-trained service engineers at your disposal whenever needed - Full automation and few moving parts assures operator independency and maximum uptime - New measuring technique reduces the waste tremendously compared to earlier methods - Minimum time needed for start-up/shut-down - Choice of capacities to suit all laboratory needs (50, 100, 150 samples per hour) - Two sensitivity levels allow high quality results on all milk qualities - Reagents are mixed and supplied to the instrument in just a few minutes - Measure cold or warm samples without any pre-treatment - Few or many samples per day - we supply reagents to suit your needs Analytical quality assurance! - Advanced error handling detects mechanical malfunctions before any faulty results are measured - A Bacterial Control assures and documents correct performance - Quality Assurance Application software offers current information on performance and helps trouble shooting if needed Correct analysis for payment at the lowest possible price! - High labour force flexibility by using the same software and sample handling as the Fossomatic and MilkoScan instruments for raw milk analysis - Improved data handling and safety in results - Full up gradability and a number of options in common with Fossomatic and MilkoScan With BactoScan FC you will: - Be running your first batch within 15 minutes - Finish mixing reagents in 5 minutes - Be able to shut down the instrument in less than 5 minutes - Get maximum economic benefits by maximising analysis capacity and minimising operating costs - all compared to traditional methods and earlier BactoScan versions And running on cold samples allows one operator to handle 2 or 3 instruments, even at 150 samples per hour. Compared to all other alternatives, this means better business for you.

4 BactoScan FC, a succ ess made even better! We asked experienced BactoScan 8000 users where they saw major areas of improvement in a new generation BactoScan. The 5 most important points were: Analytical quality assurance with a stable control sample Rapid start-up procedure Flexibility in kit sizes Low running and service costs Environmental protection These have been major focus areas for improvement throughout the development of BactoScan FC. So now we introduce: Reagent mixing in minutes and increased security - Only one active reagent to be mixed, add the enzyme to the staining stock solution - and that's it! - All liquids are filtered by the instrument just before use to avoid contamination from reagents - Filters are replaced in seconds or at service overhauls Flexibility when purchasing reagents The very simple reagent concept allows much more flexibility when purchasing reagents: - All reagents can be supplied in packages for approx. 25,000 samples (150 samples/hour) - In addition, the enzyme stock solution is supplied in smaller volumes to comply with varying needs - All reagents are ordered separately This leads to the most competitive analysis system when compared to earlier BactoScans and in particular to traditional plate count methods. Environmental protection BactoScans produce only a minor amount of waste compared to traditional methods. Compared to the earlier generations the BactoScan FC will even further reduce the environmental load: - by a 50% reduction in the total amount of waste per sample - by a 90% reduction in the amount of staining medium - by an 85% reduction in packing materials Open the lid, - and find a new world! The cabinet of the BactoScan 8000 was a revolution when it was introduced, easing surveillance and service tremendously. In the BactoScan FC, the wet area with reagents and the dry area with the electronics are separated from each other, with two lids for easy access. This means very easy service and maintenance, and higher reliability. FOSS offers an instrument that is easy to maintain and keep dry and clean, and with the fewer moving parts, you get a robust instrument that does not need daily adjustments. Modern electronics An external PC controls the whole instrument and stores the results automatically. A CD contains all software and user documentation and will guide the operator through the use of the instrument. Remote diagnostic facilities will help the operator monitor instrument performance on-line together with a remote service engineer when needed.

5 Instant bacteria counting based on flowcytometry The principle: By incubating the milk with a unique incubation liquid and by employing mechanical treatment, all components in the milk are broken down except for the bacteria. The bacteria clusters are also separated into single bacteria. At the measuring point, the sample material is exposed to a light beam from a laser source, forcing the stained bacteria to emit red light, - one light pulse for every bacteria passing the beam. The electronics count the pulses and display them in a Pulse Height Analysis diagram on the PC monitor. Electronics During the incubation period, the bacteria are stained with a DNA-specific staining medium. A very precise syringe is used to pass the whole sample through a flowcell, presenting the bacteria one by one. Technological innovation The entire history of the BactoScan is about outstanding technological innovation. And with the BactoScan FC this is again certainly the case. The unique combination of technology and biochemistry, combined with optimum data processing, makes the BactoScan FC a winner. The new reagent concept, together with the two sample homogenisers, allows the entire sample treatment to take place in the incubation unit, no sample separation is required. A specifically designed flow cell and laser technology are the final elements that allow the BactoScan FC to run up to 150 samples per hour! Instant results In contrast to other traditional methods of bacteria counting, the BactoScan counts the bacteria directly, without the need for incubating the sample to grow the bacteria colonies. Complies well with the reference method Thanks to the high precision and the counting of only viable bacteria, the accuracy of the Bacto- Scan FC is excellent compared to the reference method. The 100% automated BactoScan specifically counts the individual bacteria cells (IBC), offering repeatable and robust results. This gives a high accuracy compared to a method which is strongly influenced by the operator and by the number of single bacteria in a bacteria cluster. Experiments have shown large variation in plate count results depending on the treatment of the milk sample on its way to the laboratory. With plate count, you may count 3 on this sample,.. or 7 if the sample has been shaken a bit, Unique precision The unique reagent concept and a highly efficient stirrer enables direct test of samples - without prior heating or dilution that sets back precision. The fact that BactoScan FC counts bacteria as single cells rather than clusters lifts its repeatability to a higher level. And reproducibility is extremely good as well, due to a combination of our careful instrument design, FOSS Quality Control and the Bacterial Control that ensures instrument standardisation worldwide. This, plus a sequence of fully automated operations, lies behind the analysis time of less than 10 minutes, and a total throughput of 150 samples per hour... but BactoScan FC will count the correct number which in this case is 12!

6 For Integrated Milk Testi ng, choose BactoScan FC The BactoScan FC is a true member of the Integrated Milk TestingTM family from FOSS. This implies that a number of options available on our CombiFoss, MilkoScan and Fossomatic units are also available on the new BactoScan FC. The great advantage of this from a laboratory point of view is that the same software, the same conveyors, barcode readers etc. can be used throughout the laboratory. This eases training of laboratory staff and facilitates data transfer and -handling. The external PC with Windows Operating System and the CD-ROM supplied from FOSS eases the introduction of new PC technology. As we guarantee: - full compatibility and upgradeability you can rest assured that your FOSS equipped laboratory will be in the front-line for many years to come. 10 good reasons for investing in the new BactoScan FC: 1 2 Fully reliable results within 10 minutes The fully automated instrument is basically 100% operator independant 3 Choose between three capacities from 50 to 150 samples per hour 4 Two different sensitivities are offered 5 Control samples allow complete surveillance of instrument performance 6 Simple, quick and safe reagent handling 7 IMT System 4000 software in common with other FOSS milk analysers 8 Remote diagnostics 9 Service overhaul once a year/2000 h ours 10 Spare parts supply guaranteed for at least 7 years after production is stopped TM - All adding up to large reductions in total costs per sample and with superior laboratory performance on top! Flexibility through modularity: Free Fatty Acids Casein ph Freezing Point Urea Citric Acid Solids Lactose True Protein 500 Crude Protein 400 Fat 300 IMT Options: Software Bar Code Reading Graphical Monitoring Advanced Network Options: High Sensitivity Low Sensitivity 500 Quality Check System Quality Check System Remote Diagnostics Precision Setup Ny tegning fra MSC FT 6000!!! MilkoScan FT 6000 Basic Module 200 Fossomatic 5000 Basic Module Capacity 200 BactoScan FC Basic Module Capacity 50 Capacity Pipette Pipette BSC FC Output Unit Unit Pipette Buffer Extension Table Bar Code Scanner Conveyor Bottle Rotate Unit Table Ext. PC Extension Printer FOSS is dedicated to the dairy industry Todays' Central Milk Testing Laboratories are highly automated, and these laboratories often run thousands of samples every day. FOSS has grown up together with these Laboratories, your industry being our major business area, and FOSS is by far the largest supplier of milk analysis equipment. This, we believe, assures a common interest in optimising our mutual business. Our challenge is to fulfill your needs in a simple and cost efficient manner. Your instruments must work year in and year out, all over the world. Sofar we have succeeded, as 9 out of 10 choose We work together with our customers our solutions, and we have more than 40 years of experience in milk analysis. Whereever your laboratory is situated, we are not far away. We have our own Sales and Service Companies in 21 countries worldwide and representatives in more than 50 other countries, all employing competent, factory-trained sales and service staff. FOSS works in close cooperation with our customers when planning, designing and introducing new solutions for the dairy industry. Through individual meetings, expert committees and industrial seminars, we maintain a close dialogue in order to get the best possible background for our development projects, and to ensure that you have influence on your future analysis equipment.