SYLLABUS PRBS 132 BIO SCI

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1 CENTRO ESCOLAR UNIVERSITY Manila*Malolos*Makati DEPARTMENT OF BIOLOGICAL SCIENCES SYLLABUS PRBS 132 BIO SCI 132 Molecular Biology/Biotechnology 5 3 hrs. lec; 6 hrs. lab Subject Code Subject Title Descriptive title Credit Unit (s) Hour(s)/ Week Course Description The course deals with the study of molecular biology as it relates to the current and future practice of the science of biology. It also includes the important topics for molecular biology and their application in biotechnology, human genomic structure, and human genetic diseases and treatments for a better understanding. Emphasis will be placed on recombinant DNA, forensics and molecular genetics, especially as they pertain to medicine, agriculture and the environment. Laboratory activities will be provided to practice creative and critical thinking when observing various experiments, like DNA extraction and purification, bacterial transformation, DNA fingerprinting by PCR,restriction enzyme analysis, micrometry and cell densities, thin layer chromatography, column chromatography, Isolation of chromosomes, protein assay, bacterial plasmid isolation and genetic expression and immune detection. The students taking this course will come away with an intimate conception of the techniques used in today s developing biotechnology industry. Pre-requisites: Botany 11, Zoology12, Bio Sci 11, 12 & 14 Course Objectives At the end of the course, the students 1. discuss the basic concepts in Molecular biology and Biotechnology 2. explain the importance and use of DNA manipulation in today s world 3. describe human genetic structure and function, the basis for diseases of genetic origin, basis of analytical tests developed using biotechnology 4. manifest practical skills in DNA manipulation 5. apply the fundamental biological techniques and skills through practical experiences in the laboratory 6. follow the scientific research project and be able to report its results in a research format, and 7. value the promising biotechnology products, or new agricultural processes. 8. demonstrate behavior that is consistent with the core values of the university and that of the School of Science and Technology. Page 1 of 11

2 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures 1. trace the development of molecular biology 2. enumerate the contributors and discuss their contributions for the development of Molecular biology 3. relate Cell biology, Genetics and Biochemistry to Molecular Biology, and 4. manipulate and be familiarized with the use of the different scientific instruments. Unit I- Introduction to Molecular Biology 1. History of the development of Molecular Biology. 1.1 Evolution and the cell theory 1.2 Classical Biochemistry and Genetics 1.3 The Merging of Genetics and Biochemistry 1.4 The Birth of Molecular Biology Laboratory: Familiarization of the different equipments to be used in the performance of the experiments. Micropipettes, Centrifuge, UV-VIS Spectro - photometer, AGE Agarose Gel Electrophoresis Library and research work Socialized discussion Hands- on usage and operations of the different scientific equipments. 3 hours ( lec) Quizzes Research work (Graded of Rubrics) Recitations Practical Exam 1. discuss the concepts about the molecules of the cell 2. compare and contrast the composition, function and structure of the different biological molecules, and 3. appreciate the importance of the different biomolecules in the cell and to the organism as a whole. Unit II. The BioMolecules in the Cell 2.1 Classification of Molecules by function 2.2 Four families of Biological Molecules Composition, Function and Structure Carbohydrates Composition, structural features, Examples, and functions Power point Presentation Class Discussion with use of Multimedia Instructional materials Reporting 3 hours(lec) Assignments Recitations Quizzes Report Page 2 of 11

3 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures Lipids Composition, structural features, examples, and functions Proteins Composition, structural features, examples, and functions Nucleic Acids: DNA and RNA Power point Presentation Class Discussion with use of Multimedia Instructional materials Reporting 3 hours(lec) 2.3 Enzymes Properties, Functions, Types and Examples Laboratory: 1. Spectrophotometric Analysis of Carbohydrates 2. Spectrophotometric Analysis of Protein 3. DNA / RNA Extraction Performance of the suggested laboratory experimentsreporting of the results Graded reports Assessment of written output Quizzes should be able to: 1. differentiate the roles of the three RNAs 2. explain the rules for the synthesis of proteins and nucleic acids 3. describe the synthesis of proteins and of the nucleic acids 4. illustrate and construct DNA and RNA model, and 5. appreciate the importance of DNA and RNA in relation to the understanding of Molecular Biology. Unit III. Synthesis of Proteins and Nucleic Acids 3. Protein Synthesis 3.1 The three roles of RNA 3.2 Steps in Protein Synthesis 3.3 Rules for the synthesis of proteins 3.4 Rules for the synthesis of nucleic acids PowerPoint presentation Class Discussion with use of Multimedia Instructional materials Reporting Model making of RNA and DNA structure 3 hours (lec) Quizzes Graded recitation Model Presentation Report Page 3 of 11

4 Specific Objectives Course Content Teaching strategies Time allotment Evaluation Measures should be able to: Unit IV. Principles of Cellular Organization Quizzes 1. compare and contrast the macromolecules of both prokaryotes and eukaryotes 2. identify and describe the differences and amount of DNA per cell of different organisms 4. 1 Macromolecules in Prokaryotic Cells 4.2 Macromolecules in Eukaryotic cells 4.3 Eukaryotic Chromosomes and Genes Chromosomal Proteins: Histones and Nucleosomes The Biological and Molecular Definition of Gene PowerPoint presentation Class Discussion with use of Multimedia Instructional materials Reporting 3 hours (lec) Graded recitation Report 3. explain the organization of DNA in both prokaryotes and eukaryotes, and 4. perform recombination and complementation tests that will improve gene definition. 1. discuss gene expression, structure and replication in Prokaryotes Laboratory: 1. Growth of Microorganisms and Cell Cultures 1.1 Conjugation, Transduction and Transformation of genes in Bacteria 1.2 Plasmid DNA Extraction from Bacterial Cell 1.3 Transformation of Eukaryotic Cells Unit V. RNA Synthesis and Gene Control in Prokaryotes 5.1 Transcription of DNA and Translation of mrna in Prokaryotes 5.2 The Strategy of Prokaryotic Gene Control Performance of the suggested laboratory experiments Reporting of the results Class Discussion Research and Library Work Visual Presentation Individual Reporting 3 hours (lec) Quiz Reports Assessment of written output Quizzes Individual report Page 4 of 11

5 Specific Objectives Course Content Teaching strategies Time allotment Evaluation Measures 2. compare the primary and secondary structures of rrna and mrna for a ribosomal protein 5.3 Negative Control of Transcription: The Lactose Operon 5.4 Positive Control of Transcription 5.5 Compound Control of Transcription in Several Operons 5.6 The Control of Regulatory Proteins 5.7 Control of the Termination of Transcription in Bacteria 5.8 The Stability of Biopolymers in Bacterial Cells should be able to: 1. discuss gene expression in eukaryotes 2. establish comparison of gene expression between prokaryotes and eukaryotes Unit VI. RNA Synthesis and Processing in Eukaryotes 6. 1 The RNA polymerases and General Transcription Factors of Eukaryotic Cells 6.2 The Structure and Biosynthesis of Eukaryotic mrnas 6.3 The Biochemical Signals for the Steps in mrna Biogenesis 6.4 Proteins Associated with hnrna Class Discussion Research and Library Work Visual Presentation Individual Reporting 3 hours (lec) Quiz Individual report Page 5 of 11

6 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures 6.5 Nuclear Pores. The Exit sites of mrna 6.6 The Appearance of mrna in the Cytoplasm and the Initiation of Protein Synthesis 1. discuss the purpose of gene control in unicellular and multicellular organisms, and 2. describe the three components of gene control Unit VII. Gene Control in Eukaryotes 7.1 The purpose of Gene Control in Unicellular and Multicellular Organisms 7.2 The components of Gene Control: Signals, Levels, and Mechanisms Signals for Gene Control in Eukaryotic cells Levels of Regulation in mrna metabolism Transcriptional Control and how it is Demonstrated in Eukaryotes Gene Control in the Cytoplasm Mechanism of Transcriptional Control: Site-Specific DNA Binding and Changes In Chromosomal Topology Class Discussion Research and Library Work Visual Presentation Individual Reporting 6 hours (lec) Quiz Individual report Reflection Journal Page 6 of 11

7 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures Unit VIII. Gene Regulation 1. explain gene regulation 2. differentiate gene regulation of prokaryotes to eukaryotes 3. compare and contrast transcriptional and translational regulation, 4. cite examples of gene regulation, and 5. explain the role of gene regulation in development and in evolution 8.1 Molecular Definition of Gene Regulation Gene Regulation in eukaryotes Gene Regulation in prokaryotes Stages of Gene Regulation 8.2 Epigenetic regulation (Genomic imprinting) 8.3 Transcriptional regulation post-transcriptional regulation (sequestration, alternative splicing,mirna) Class Discussion Research and Library Work Visual Presentation Concept mapping 6 hours (lec) Quiz Research work (Critic a journal; Graded with Rubrics) Concept maps 1. describe the synthesis of DNA 2. differentiate semiconservative DNA replication from bidirectional replication 8.4 Ttranslational regulation post-translational regulation (reversible,irreversible) Examples of Gene Regulation Gene Regulation in Development and Evolution Unit IX. DNA Synthesis, Repair and Recombination 9.1 DNA Synthesis Semiconservative DNA Replication Bidirectional DNA Replication 9.2 Properties of DNA Polymerases and DNA in Vitro Class Discussion Research and Library Work Visual Presentation Individual Reporting 6 hours (lec) Quiz Individual report Page 7 of 11

8 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures 3. explain the importance of topoisomerases, and 4. explain how repair is done in DNA 5. describe how recombinants DNA are formed 9.3 DNA Topoisomerases 9.4 Mechanism and Sites of Initiation in DNA Synthesis 9.5 The Assembly of DNA into Nucleosomes 9.6 Repair and Recombination of DNA 1. describe the molecular definition of gene mutation 2. differentiate the possible ways on how gene mutation occurs 3. explain the possible causes of mutation, and 4. compare and contrast the different types of mutation Unit X. Gene Mutation 10.1 Molecular Definition of Gene Mutation 10.2 Gene Mutation occurs in four ways: Hereditary Mutation Acquired Mutation New(de Novo mutations) Class Discussion Research and Library Work Visual Presentation Concept Mapping 6 hours (lec) Quiz Reflection Journal Concept Map Mosaism 10.3 Causes of Mutation 10.4 Classification of the types of Mutation Page 8 of 11

9 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures Unit XI- Experimental Manipulation of Macromolecules 1. describe the experimental manipulation of the different recombinant DNA 2. illustrate the different processes in Recombinant DNA 3. Discuss the detection of recombinant DNA molecules and PCR primers discuss the in relation of Molecular Biology. 4. Appreciate the importance of the roles of genetic engineers 1I.1 Recombinant DNA 1I.1.1 Restriction enzymes function as for pinking shear 1I.I.2 Genetic Interlopers: Vectors function as vehicle for transferring genes 1I.2. Detection of Recombinant DNA molecules 1I.3 Applications of genetic engineering/ recombinant DNA technology 1I.3.1 Uses of recombinant technology 1I.3.2 Isolation 1I.3.3 Insertion 1I.3.4 Transfer of the vector 1I.3.5 Transformation 1I.3.6 Selection of the genetically modified organism (GMO) Laboratory 1. Bacterial plasmid Isolation 2. DNA Isolation 3. UV-VIS Spectrophotometric Analysis 4. Agarose Gel Electrophoresis Reporting Discussion Video presentation Performance of the suggested laboratory experiments Reporting of the results 6 hours (lec) Quiz Individual report Reflection Journal Graded reports Assessment of written output Quizzes Page 9 of 11

10 Specific Objectives Course Content Teaching strategies Time allotment Evaluative Measures 1. express understanding of the applications of biotechnology in our lives 2. appreciate and instill acceptance that progress is tied up with the innovations in Biotechnology Unit XII. Application of Biotechnology 12.1 In Medicine 12.2 Pharmacogenomics 12.3 Pharmaceutical products 12.4 Gene testing 12.5 Gene Therapy 12.6 Human Genome Project Reporting Discussion Video presentation 3 hours (lec) Quiz Individual report Research work (Critic a journal; Graded with Rubrics) 12.7 Cloning Page 10 of 11

11 Textbooks/References: Alberts, Bruce et al. Essential Cell Biology, 3 rd ed. Garland Science Alberts, Bruce, et.al. Molecular Biology of the Cell.. 5 Th ed Garland Science, New York, USA Chandar, Nalini. Lippincott s Illustrated Reviews: Cell and Molecular Biology. Lippincott Williams & Wilkins,2010 Copper, Geoffrey M. The Cell: A Molecular Approach 5 th ed. Sinauer Associates, Inc Karp,Gerald. Cell and Molecular Biology: Concepts and Experiments. 6 th ed. USA: John Wiley & Sons,Inc.,2008. Lodish, Harvey et al. Molecular Cell Biology, 7 th ed. W.H. Freeman, Rastogi, Veer Bala. Fundamentalas of Molecular Biology. ANE Books Weaver, Robert. Molecular Biology 4 th ed, McGraw-Hill Science/Engineering/Math Electronic references: Prepared by: Approved by:. Sgd. Dr. Julieta Z. Dungca Sgd. Dr. Zenaida DR. Los Baños Chair, Faculty Committee on Preparation/Review of Syllabus Head, Biological Sciences Department (Molecular Biology) April 2014 Biological Sciences Department Page 11 of 11

12 RUBRIC FOR MODEL PRESENTATION EXCELLENT VERY GOOD GOOD POOR CRITERIA Model Displays all relevant information Missing of 1-2 important information Missing 2-3 relevant information Missing of 4 or more relevant information Accuracy/Content Materials used are appropriate and presentation can easily be understand All members of the group participated Submitted on or before the set deadline Materials used are appropriate by cannot easily be understand 1 group member did not participate Submitted 1 day after the set deadline Materials used are less appropriate and presentation can not be well understood 2 group member did not participate Submitted 2 days after the set deadline Materials are appropriate and presentation can not be understood 3 group member did not participate Submitted 3 days after the set deadline Materials Used Group Presentation Submission RUBRIC FOR INDIVIDUAL ORAL REPORT: (20/20) EXCELLENT VERY GOOD GOOD POOR GRADE Topic is 100% relevant. Topic is 95% relevant Topic is relevant Topic is not relevant at all. Topic is very relevant (5) Delivery of report is 100% Delivery of report is 95% Delivery of report is 90% Delivery of report is 100% Delivery of report (5) organized, systematic organized, systematic organized, systematic disorganized. Language used and Grammar 100% correct. 1-2 Taglish, and grammar errors. 3-4 Taglish and grammar errors 5 or more Taglish and grammar errors. Language (5) 100% voice sound is audible, articulate, projection 1-2 voice sound inaudible, articulate, lost projection 3-4 voice sound inaudible, projection 5-6 voice sound inaudible, projection Voice projection (5) More than 5 students asked questions 4 students asked questions 3 students asked questions 2 or no students asked questions Interactive (5) Page 12 of 13

13 RUBRIC FOR REACTION/REFLECTION PAPER: (20/20) EXCELLENT VERY GOOD GOOD POOR GRADE CRITERIA Content is 100% related on the Content 98% related on the Content 90% related on the Content is not related at all. Content (5) assigned topic. assigned topic. assigned topic. Exposition is coherent and Exposition is coherent but not Exposition is not coherent, Exposition is with not Exposition (5) with no flaws. systematic. with missing point and disarray. coherent, disarray and with no sense. No error on grammar 1-2 errors in grammar 3-4 errors in grammar 5 or more errors in grammar. Grammar (5) Date of submission ahead of the deadline. Submitted on the date of submission 1 day late on the deadline 2 days late on the deadline Date of submission (5) RUBRIC FOR RESEARCH PAPER: (100/100) EXCELLENT VERY GOOD GOOD POOR GRADE CRITERIA Research output for the subject Research output for the subject Research output for the subject Research output for the subject Value of the research (20) is 100% relevant, feasible for the good of humanity. is 98% relevant, feasible for the good of humanity. is 94% relevant, feasible for the good of humanity. has no relevance at all. Research output is 100% Research output is 95% Research output is duplicative, Research output has no Originalitiy (20) original. Research paper is 100% in grammar and presentation, follows the standard being used by the research community, APA format. Research output is publishable in the University Journal Research output is 100% competitive original. 1-2 errors in grammar, deviating in APA format Research output is 95% publishable in the University Journal. Research output is 95% competitive. validating. 3-4 errors in grammar, deviating in APA format Research output is not putblishable. Research output is not competitive. originality. 5 or more errors in grammar, deviating in APA format. Research output is not publishable. Research output is not competitive. Paper write-up (20) Publication (20) Presentation (20) Page 13 of 13

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