SDS PAGE with unsurpassed resolution and 2 year shelf life!

Transcription

1 ClearPAGE Precast Gels SDS PAGE with unsurpassed resolution and 2 year shelf life! Choice of 3 Cassette Sizes 10cm(w) x 8cm(h) 10cm(w) x 10cm(h) 15cm(w) x 10cm(h) 12% ClearPAGE SDS precast gel, 15cm(w) x 10cm(l), wells Marker Lanes, Chicken Lysate, Lanes 1, 4, 5, 6, 7, 11, 12, Chicken Lysate, Lanes 2, 10, BSA markers, Lane 8, E.Coli, Lanes 3, 9 BioRad Markers SPECIFICATIONS Cassette Size W x L (cm) COMPATIBLITY Cassette Size W x L (cm) Gel Dimensions W x L (cm) Gel Box Manufacturer 10 x 8 Bio-Rad Bio-Rad C.B.S. Scientific C.B.S. Scientific 10 x 10 C.B.S. Scientific C.B.S. Scientific Invitrogen Lonza 15 x 10 C.B.S. Scientific Invitrogen Gel Thickness Shelf Life Compatible Gel Boxes Mini-Protean II * Mini-Protean Tetra * Mini-Vertical MGV-302 Dual Mini-Vertical MGV-402 Dual Cool DCX-700 Quadra Systems QNX-700, QNC-700 X-cell PAGEr Minigel Chamber DCX-800 Xcell4 Required Buffer 10 x x 1.0mm 2 years Tris-Tricine 10 x x mm 2 years Tris-Tricine 15 x x mm 2 years Tris-Tricine FEATURES Very strong gel makes handling easy. 2 year shelf life - never throw away another gel! Unique acrylamide polymer matrix for exceptional protein separations Sharp, clean, consistently straight bands There are no combs or tape to remove shortening prep time. Reusable lower buffer saves time and money. You can save up to 75% of the buffer cost. All gels have the ability to expand the resolving range of a given gel by running the small proteins off the unsealed bottom of the gel. Long well fingers are fixed and extend above top of cassette for easy loading and to prevent sample crosscontamination. Red dye is used in the loading area for better well visualization in the 8cm x 10cm and 10cm x 10cm sizes. Simple Tris-Tricine buffer system. NOTE: Tris-Tricine buffers MUST be used with these gels. *requires replacement gasket # BRGK-100. COMPATIBLE WITH: C.B.S. Scientific Electrophoresis Systems Dual Vertical Midi-Gel, Cat. No. DCX-800, fits 15 x 10cm gels Quadra Systems, Cat. No. QNX-700 & QNC place Combo Cells fits 10 x 10cm gels Dual Cool, Cat. No. DCX place Combo Cell with freezer blocks fits 10 x 10cm gels Dual Mini-Verticals with internal cooling Cat. No. MGV-202 fits 10 x 10cm gels Cat. No. MGV-402, fits 10 x 8cm gels Mini-Blotter Cat. No. EBU-204 with cooling base, 4-place fits 8cm x 10cm and 10 x 10cm gels Midi-Blotter, Cat. No. EBU-402 cooling base, 2-place fits 10 x 15cm gels ClearPAGE is a trademark of C.B.S. Scientific. PAGEr and Lonza are trademarks of Lonza Corporation. Surelock and XCell are registered trademarks of Life Technology Corporation. Mini-PROTEAN and Tetra are registered trademarks of Bio-Rad Laboratories Corporation.

2 CLEARPAGE GEL SELECTION CHART ClearPAGE SDS gels- TEO, packages of 10 Acrylamide % Gel Size (cm) W x H 1 well 2D well trough + 1 marker lane 12 well 12 well + 2 marker lanes 17 well 20 well 8% 10 x BK BK x FK FK % 10 x BK BK x10 FK * - FK FK % 10 x BK BK x10 FK * - FK FK well 15 x GK GK GK % 10 x BK BK x FK FK % 10 x BK BK x FK FK % 10 x BK BK x FK FK x GK GK GK % 10 x BK BK *Minimum order of 30 gels 10 x10 FK41-10* FK42-10* FK FK x GK GK GK Gel type, expiration date, and lot number CASSETTE FEATURES Finger-locking cassettes Snap-fit cassette opens easily Comb Load Volumes Well Type Cassette Size (cm) 12-well 8 x x 10 Maximum Load Volume 35 µl 12-well + 2 marker lanes 10 x µl + 17µl (marker) Long numbered well fingers extend above top of cassette to prevent sample carryover. Red dye is used in the loading area for better well visualization. This dye runs ahead of the bromphenol blue marker. 17-well 8 x x µl 20-well 10 x µl 26-well 10 x µl 2-D well trough + 1 marker lane 10 x µl + 17µl (marker) Prep-Well (1 well) 10 x µl Separation Range Polyacrylamide Concentraion Size Separation Range 8% - kda 10% 15- kda 12% 10- kda 16% 5-60 kda 4-8% kda 4-12% kda 4-20% 5- kda phone: (858) Toll Free: (800)

3 ClearPAGE Precast Gels ClearPAGE GEL FEATURES Long Shelf Life: Never throw away expired gels again! These TEO gels use a neutral ph gel buffer to reduce alkaline hydrolysis. Combined with reduced polymer swelling, all gels, including high percentage gels, carry a Best By Date of 2 years from the date of manufacture. They show no change in the separation when stored at 4 C during that period, and will give useful results for years afterward. The gels also may be stored at room temperature (up to 25 C) for 3 months after receipt. Incredibly strong & elastic gels: New polymer and polymerization technology are used to make highresolution, cross-linked polyacrylamide gels combined with a small amount of agarose in plastic cassettes without a coating. These gels are about 10 times stronger than the equivalent polyacrylamide gel without agarose, so they can be handled, stained, and dried without tearing or cracking, and blotted without sticking to your blotting membrane. Buffer Systems: The Tricine SDS reducing Buffer (cat. # FB60500) is the most popular based on migration and speed. This buffer is also recommended for antibody separations. Use ClearPAGE Turbo SDS Running Buffer (catalog #FB14053 and FB100) to shift the gel separation range so as to increase spacing of smaller proteins. Gels run about 40% faster with Turbo running buffer. This buffer contains SDS, tris and MOPS, with 5mM sodium bisulfite. Better resolution and sensitivity: The gel technology in ClearPAGE products provide robust separations superior to other products on the market. Improved stacking allows large sample volumes so that 1mm ClearPAGE cassette gels have the equivalent sensitivity to 1.5mm gels. With the ClearPAGE team members broad experience developing and making commercial pre-cast gels and our commitment to quality, you can be assured of high-quality, consistent performance every time. Gasket Replacement on Bio-Rad Units: To use ClearPAGE Precast gels with Bio-Rad Mini-Protean Gel Boxes the green gasket is easily replaced with a white gasket (cat. # BRGK-100) provided by C.B.S. Scientific. 1) Pull green clamps away from sides of reservoir to access green gasket. 2) Pull green gasket out of groove. 3) Place white gasket into groove so that tapered side faces out. Your Mini-Protean II or Mini-Protean Tetra is now ready to accept ClearPAGE 8x10cm precast gels Instant Blue A fast one-step stain Easy to Use: Remove SDS, fix and stain in ONE STEP. No destain is needed. Fast: Results in 10 to 30 minutes Sensitive: 5-25ng of protein can be detected with low background Non-Hazardous: Contains no methanol, suited for regular use and disposal DESCRIPTION Instant Blue stain is a ready-to-use, proprietary Coomassie G-250 stain that is optimal for obtaining well defined protein bands in a single step. Protein gel staining takes only minutes because there is no need to wash, fix, microwave or destain. Instant Blue stain contains a proprietary SDS-sequestering agent (patent pending) that makes it so fast and easy. Since SDS binds stain, it normally must be removed to get good sensitivity. Usually some remains in the gel, which contributes to background and requires destaining. The SDS-sequestering agent removes the SDS from the gel, so no washes or destaining is necessary. Its special formulation has the benefits of high sensitivity (approximately 5-25ng of protein detected per band) and low background interference-improving the overall gel resolution. Additionally it contains no methanol and is ideally suited for regular use and disposal. Two-Color SDS Marker An orange/blue prestained marker for SDS-PAGE Stable and Sharp Recombinant Proteins Economical - Use in place of unstained markers DESCRIPTION The recombinant proteins range in size form 7.6 kda to 195 kda. The orange markers are 28 kda and 71 kda, the other 8 bands are blue. The ClearPAGE Two-Color SDS Marker is sold ready to use in a 1x LDS sample buffer with 600 µl per vial (catalog # HM05160). The purity and band sharpness mean the markers can be used for visualizing the separation while running the gel, checking effectiveness on your blot, or as a routine marker in place of other unstained markers. Natural kda Instant Blue Stained Comparison of Marker on ClearPAGE 12%, 12 well SDS gel with or with Instant Blue stain. Molecular weights are the apparent weights in the ClearPAGE SDS gel system.

4 ClearPAGE 10cm x 10cm Precast Gel Migration Chart in kda Turbo Buffer - FB100 SDS Single % Gels SDS Gradient Gels 8% 10% 12% 16% 4-8% 4-12% 4-20% ClearPAGE 10cm x 10cm Precast Gel Migration Chart in kda Tricine Buffer - FB60500 SDS Single % Gels SDS Gradient Gels 8% 10% 12% 16% 4-8% 4-12% 4-20% ClearPAGE 8cm(h) x 10cm(w) Precast Gel Migration Chart in kda Turbo Buffer - FB100 SDS Single % Gels SDS Gradient Gels 8% 10% 12% 16% 4-8% 4-12% 4-20% 1.00 phone: (858) Toll Free: (800)

5 ClearPAGE Precast Gels ORDERING INFORMATION ClearPAGE SDS gels- TEO, 10cm(w) x 8cm(h), packages of 10 If using BioRad Mini-Protean order gasket adaptor kit BRGK-100. Acrylamide % # of Wells 8% 12 BK % 17 BK % 12 BK % 17 BK % 12 BK % 17 BK % 12 BK % 17 BK % 12 BK % 17 BK % 12 BK % 17 BK % 12 BK % 17 BK ClearPAGE SDS gels- TEO, 10cm(w) x 10cm(h), packages of 10 Acrylamide % # of Wells 8% 12 FK % 17 FK % 1 FK % 12 FK % 17 FK % 1 FK * 12% 12 FK % 17 FK % 12 FK % 17 FK % 12 FK % 17 FK % 12 FK % 17 FK % 1 FK41-10 * 4-20% 2D + 1 marker lane FK42-10* 4-20% 12 FK % 17 FK ClearPAGE SDS gels- TEO, 15cm(w) x 10cm(h), packages of 10 Acrylamide % # of Wells 12% GK % 20 GK % 26 GK % GK % 20 GK % 26 GK % GK % 20 GK % 26 GK ClearPAGE Buffers, Stains and Markers Description Quantity LDS Sample Buffer 2 ml FB002 LDS Sample Buffer 10 ml FB010 DTT reducer, 10X 1 ml FB SDS-non-reducing running buffer 50 ml FB50053 SDS-non-reducing running buffer 0.5L FB50500 Tricine SDS reducing running buffer 50 ml FB60053 Tricine SDS reducing running buffer 0.5 L FB60500 Turbo buffer SDS reducing running buffer 50 ml FB14053 Turbo buffer SDS reducing running buffer 0.5 L FB100 Transfer buffer, with SDS 0.5 L FB82500 Transfer buffer, without SDS 0.5 L FB82600 Instant Blue stain 1 L HG73010 Instant Blue stain, trial size 50 ml HG color marker 0.6 ml HM color marker, trial size 30 µl HM05103 ClearPAGE Blotting and Drying Description Quantity TGS transfer buffer, 10x 500 ml FB82500 Blot sandwich NC 90 x 85mm, 0.2µl nitrocellulose/1mm blotting paper Blot sandwich NC 90 x 85mm, 0.2µl nitrocellulose/1mm blotting paper Blot sandwich PVDF 90 x 85mm, 0.2µl PVDF/1mm blotting paper Blot sandwich PVDF 90 x 85mm, 0.2µl PVDF/1mm blotting paper 2 pack HP pack HP pack HP pack HP29320 Gel drying solution 1 L HB010 Precut cellophane 20 sheets HB02520 Drying frame with 8 clamps with cellophane ClearPAGE Sampler Kits 20 pieces of cellophane HB90001 Description ClearPAGE Sampler Kit, Includes 2 each 10cm x 10cm SDS gels of customer s choice, trial buffers and instructions. ClearPAGE Sampler Kit, Includes 2 each 10cm x 10cm DNA/Native gels of customer s choice, trial buffers and instructions. ClearPAGE Super Sampler Kit, Includes 2 each 10cm x 10cm SDS gels of customer s choice, trial buffers, trial size 2-color marker, trial size Instant Blue stain and instructions. ClearPAGE Super Sampler Kit, Includes 2 each 10cm x 8cm SDS gels of customer s choice, trial buffers, trial size 2-color marker, trial size Instant Blue stain and instructions. ClearPAGE Super Sampler Kit, Includes 2 each 15cm x 10cm SDS gels of customer s choice, trial buffers, trial size 2-color marker, trial size Instant Blue stain and instructions. FM90002 FM90003 FM90005 FM90006 FM90007 * minimum order of 30 gels

6 ClearPAGE Precast DNA/Native Gels DNA/Native ClearPAGE Precast Gels with unsurpassed resolution and 2 year shelf life Choice of 2 Cassette Sizes 10cm(w) x 8cm(h) 10cm(w) x 10cm(h) ClearPAGE 10cm(h) x 10cm(w) DNA/Native Precast Gel Migration Chart in BP DNA/Native Buffer - GB61500 Gel Percentage 2-8% 10% 3-20% 3000 bp Superior DNA Separations: Precast DNA and Native PAGE gels also have a long shelf life. These gels are a neutral ph and utilize a stacking system. Larger sample volumes (such as 35 µl/12 well and 17 µl/17 well) will still produce sharp bands bp 0 bp 1500 bp 1 bp 1000 bp 0 bp 1500 bp 1 bp 1000 bp 700 bp 500 bp 400 bp 3000 bp 0 bp 1500 bp 1 bp 800 bp 700 bp 500 bp 400 bp 300 bp FEATURES Unique acrylamide polymer matrix for exceptional separations Sharp, clean, consistently straight bands There are no combs or tape to remove shortening prep time. Super-strong gel makes handling easy. Reusable lower buffer saves time and money. You can save up to 75% of the buffer cost. TEO gels have a neutral ph for a 2 year shelf life - never throw away another gel Long well fingers extend above top of cassette and to prevent sample cross-contamination. Green dye is used in the loading area for better well visualization. Simple Tris-Tricine buffer system. NOTE: Tris-Tricine buffers MUST be used with these gels. SPECIFICATIONS Gel type Shelf life Overall Cassette Dimensions Gel Dimenions DNA/Native gels 2 years 8(h) x 10(w) x 5mm(d) 10(h) x 10(w) x 5mm(d) cm(h) x 8.5cm(w) x 1mm(d) 8.0cm(h) x 8.5cm(w) x 1mm(d) Number of Gels / Box 10 Color of Gel Loading Area Gel Thickness green 1mm ORDERING INFORMATION ClearPAGE Precast DNA/Native Gel Selection Chart, packages of 10 acrylamide % 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp gel size (cm) h x w 300 bp bp 100 bp 80 bp 60 bp 12 well 17 well 10% 8 x 10 BN BN x 10 GN GN % 10 x10 GN GN % 8 x 10 BN BN x10 GN GN ClearPAGE Precast DNA/Native Gel Buffers bp 100 bp 80 bp 60 bp 40 bp 20 bp description amount GB33002 DNA/Native sample buffer, 4 x 2 ml GB33010 DNA/Native sample buffer, 4 x 10 ml GB610 DNA/Native running buffer, 20 x ml GB61500 DNA/Native running buffer, 20 x 0.5 L FB82500 Transfer buffer 0.5 L phone: (858) Toll Free: (800)