Product Datasheet. Nrf2 Antibody NBP Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet Nrf2 Antibody NBP Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 3 Publications: 7 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/17/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP Nrf2 Antibody Product Information Unit Size Concentration Storage Clonality 0.1 ml Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Polyclonal Preservative 0.025% Proclin 300 Isotype Purity Buffer Target Molecular Weight Product Description Host IgG Immunogen affinity purified PBS (ph 7), 1% BSA, 20% Glycerol 68 kda Rabbit Gene ID 4780 Gene Symbol Species NFE2L2 Human, Mouse, Rat, Avian, C. elegans, Plant Reactivity Notes C. elegans reactivity reported in scientific literature (PMID: ). Bovine (84%). Immunogen Product Application Details Applications Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary. Western Blot, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP) Recommended Dilutions Western Blot 1:500-1:3000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:100-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:100-1:500, Immunohistochemistry-Paraffin 1:100-1:1000, Chromatin Immunoprecipitation (ChIP) Images Western Blot: Nrf2 Antibody [NBP ] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP ) was used to detect the primary antibody. Page 1 of 6 v.20.1 Updated 2/17/2019

3 Western Blot: Nrf2 Antibody [NBP ] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRPconjugated anti-rabbit IgG antibody NBP ) was used to detect the primary antibody, and the signal was developed with Trident ECL plus- Enhanced. Page 2 of 6 v.20.1 Updated 2/17/2019 Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP ] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Western Blot: Nrf2 Antibody [NBP ] - Untreated (-) and treated (+) RAW264.7 whole cell extracts (30 ug) were separated by 5% SDS- PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP ) was used to detect the primary antibody. Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP ] - Human breast carcinoma. NRF2 stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, ph 6.0, 15 min

4 Western Blot: Nrf2 Antibody [NBP ] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. The HRPconjugated anti-rabbit IgG antibody (NBP ) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-enhanced. Page 3 of 6 v.20.1 Updated 2/17/2019 Western Blot: Nrf2 Antibody [NBP ] - Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody diluted by 1:1000. Immunocytochemistry/Immunofluorescence: Nrf2 Antibody [NBP ] - NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal diluted at 1:500. Blue: Hoechst staining. Scale bar = 10 um. Chromatin Immunoprecipitation: Nrf2 Antibody [NBP ] - ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-nrf2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

5 Western Blot: Nrf2 Antibody [NBP ] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal diluted at 1:500. Page 4 of 6 v.20.1 Updated 2/17/2019 Western Blot: Nrf2 Antibody [NBP ] - Untreated (-) and treated (+) MDA-MB-231 nuclear extracts (30 ug) were separated by 7.5% SDS- PAGE, and the membrane was blotted with NRF2 antibody. The observed M.W. is based on the publication: PMID: Immunoprecipitation: Nrf2 Antibody [NBP ] - Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ug of NRF2 antibody [N2C2], Internal Western blot analysis was performed using NRF2 antibody [N2C2], Internal.. EasyBlot anti-rabbit IgG was used as a secondary reagent.

6 Publications Page 5 of 6 v.20.1 Updated 2/17/2019 Choo J, Heo G, Kim SJ et al. Senescence marker protein 30 protects intestinal epithelial cells against inflammationinduced cell death by enhancing Nrf2 activity. Biochim Biophys Acta Mol Basis Dis Sep :00AM [PMID: ] (IP, Human) Tumer TB, Yilmaz B, OzleYen A et al. GR24, a synthetic analog of Strigolactones, alleviates inflammation and promotes Nrf2 cytoprotective response: In vitro and in silico evidences. Comput Biol Chem. Jul :00AM [PMID: ] (WB, Mouse) Rungratanawanich W, Abate G, Serafini MM et al. Characterization of the Antioxidant Effects of g-oryzanol: Involvement of the Nrf2 Pathway Oxid Med Cell Longev Mar :00AM [PMID: ] (WB, Human) Dehghan E, Zhang Y, Saremi B et al. Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun 2017 Dec 20 [PMID: ] (WB, C. elegans) Chung E, Joiner HE, Skelton T et al. Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts. Physiol Rep. Mar :00AM [PMID: ] (WB, Mouse) Li G, Zhang Z, Quan Q et al. Discovery, Synthesis, and Functional Characterization of a Novel Neuroprotective Natural Product from the Fruit of Alpinia oxyphylla for use in Parkinson's Disease Through LC/MS-Based Multivariate Data Analysis-Guided Fractionation. J. Proteome Res. Jun :00AM [PMID: ] Sullivan LB, Martinez-Garcia E, Nguyen H et al. The proto-oncometabolite fumarate binds glutathione to amplify ROS-dependent signaling. Mol Cell Jul 25 [PMID: ]

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