USDA Specialty Crops Research Initiative (SCRI)
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1 USDA Specialty Crops Research Initiative (SCRI)
2 USDA Specialty Crops Research Initiative (SCRI) THANKS TO YOU!!! Responding to listening sessions by federal agencies Providing information on specialty crops Help with the required 100% non-federal match Continued communications with congressional delegation
3 USDA Specialty Crops Research Initiative (SCRI) Ensuring US Onion Sustainability: Breeding and Genomics to Control Thrips and Iris Yellow Spot Virus Onion Storage Rot Management (Charlie Li) Onion ipmpipe & Diagnostics (Howard Schwartz)
4 Ensuring US Onion Sustainability: Breeding and Genomics to Control Thrips and Iris Yellow Spot Virus Project Director: Michael Havey, USDA-ARS and University of Wisconsin Project Co-Directors: Christopher Cramer, New Mexico State University Hanu Pappu, Washington State University Howard Schwartz, Colorado State University Christopher Town, The J. Craig Venter Institute Project Collaborators: Whitney Cranshaw, Colorado State University Jerry Hawkes, New Mexico State University
5 Onion Translational Genomics
6 Genomics Relatively cheap sequencing Expressed Regions Large-scale genomic sequencing High through-put analysis of all sequences Identify all putative coding regions in DNA
7 Onion Translational Genomics
8 Onion Translational Genomics Prioritize Target Traits Identify Desirable Germplasms Complete Crossing and Genetic Evaluations Develop Robust High-Throughput Genetic Markers Value-Added Cultivars
9 Onion Translational Genomics Prioritize Target Traits
10 Allium Disease and Pest Survey
11 Allium Disease and Pest Survey
12 Allium Disease and Pest Survey 100+ respondents (>>onion growers) Top Onion Production Problems in NA: Thrips Iris Yellow Spot Virus Bacterial soft rot (Erwinia) Slippery skin (Pseudomonas) Black mold (Aspergillus) Botrytis leaf blight and neck rot Fusarium basal rot Pink root Heat
13 Conference on Onion Translational Genomics
14 Overall Relevance High IYSV Thrips CMS Fusarium RNAi Flavor Carbohydrates White rot Botrytis neck rot Bacterial rots Yellow Nutsedge Flowering Black mold Herbicide tolerance Moderate Botrytis leaf blight Pink root Downy mildew Low Simple Moderate Resistant Germplasm and Inheritance Difficult
15 Thrips
16 Iris Yellow Spot Virus Photos: Fred Crowe & Hanu Pappu
17 Onion Translational Genomics Prioritize Target Traits Identify Desirable Germplasms Understand the Pest Screen the Host
18 Detection of IYSV Hanu Pappu RT-PCR ELISA
19 Mechanical Transmission of IYSV Hanu Pappu
20 Field Evaluations for Thrips and IYSV Tolerance ( ) Chris Cramer and Howard Schwartz
21
22 Field Day at NMSU in 2010
23 Promising Entries for Field Tolerance to IYSV/Thrips 2009 & 2010 (** CO and NM): PI Chile Calderana PI ** Spain PI ** USA San Joaquin PI USA Yellow Sweet Spanish PI USA Yellow Sweet Spanish NMSU USA
24 Onion Translational Genomics Prioritize Target Traits Identify Desirable Germplasms Complete Crossing and Genetic Evaluations
25 Genetic Validation of Tolerance A Line TC Family Selected Plants S 1 Family
26 Onion Translational Genomics Prioritize Target Traits Identify Desirable Germplasms Complete Crossing and Genetic Evaluations Develop Robust High-Throughput Genetic Markers
27 454 Sequencing of cdnas Chris Town and Michael Havey Red DH Yellow OH-1
28 454 Sequencing of cdnas Chris Town and Michael Havey Red DH Yellow OH-1 5 -TAGAAAAAATTGCTAACTGTTGCAGCTTGTGGGAACTGTAGTAGCAGGAAT-3 5 -TAGAAAAAATTGCTAATTGTTGCAGCTTGTGAGAACTGTAGTAGCAGGAAT-3
29 New Haploid Mapping Family Red DH x Yellow OH-1 F1 Haploid Extraction by Borut Bohanec
30 New Haploid Mapping Family Thanks to Kent Welsh
31 High Through-Put SNP Genotyping Assays: Illumina Kaspar TaqMan
32 Mapping of Target Traits
33 Association Mapping of Resistance or Tolerance to Thrips and/or IYSV
34 Ensuring US Onion Sustainability: Breeding and Genomics to Control Thrips and Iris Yellow Spot Virus Improved detection and inoculation of IYSV Tolerance to thrips and/or IYSV SNP genotyping platform and high-density map of onion Genetic tagging of semi-glossy foliage Delivery of germplasms and technologies
35 Onion Translational Genomics Useful Traits DNA Sequences Competitive Cultivars
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