Exploration of Nanoparticle-Mediated Photothermal Effect of. Quantitative Photothermal Immunoassay

Size: px
Start display at page:

Download "Exploration of Nanoparticle-Mediated Photothermal Effect of. Quantitative Photothermal Immunoassay"

Transcription

1 Analytical Chemistry Supporting Information Exploration of Nanoparticle-Mediated Photothermal Effect of TMB-H 2 O 2 Colorimetric System and Its Application in a Visual Quantitative Photothermal Immunoassay Guanglei Fu, 1 Sharma T. Sanjay, 1 Wan Zhou, 1 Rolf A. Brekken, 2 Robert A. Kirken, 3 and XiuJun Li 1,4,5, * 1 Department of Chemistry and Biochemistry, University of Texas at El Paso, 500 West University Ave., El Paso, Texas, 79968, USA 2 Hamon Center for Therapeutic Oncology Research, Departments of Surgery and Pharmacology, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, Texas, 75390, USA 3 Department of Biological Sciences, University of Texas at El Paso, 500 West University Ave., El Paso, Texas, 79968, USA 4 Biomedical Engineering, Border Biomedical Research Center, University of Texas at El Paso, 500 West University Ave., El Paso, Texas, 79968, USA 5 Environmental Science and Engineering, University of Texas at El Paso, 500 West *Corresponding author: xli4@utep.edu University Ave., El Paso, Texas, 79968, USA S-1

2 Table of Contents EXPERIMENTAL SECTION...S-3 Materials and Instruments...S-3 Preparation of Antibody-Conjugated Iron Oxide NPs......S-3 Photothermal Characterization of the Iron Oxide NPs-Mediated TMB-H 2 O 2 Colorimetric System... S-4 Iron Oxide NPs-Mediated TMB-H 2 O 2 Colorimetric Immunoassay... S-5 Specificity of the Iron Oxide NPs-Mediated TMB-H 2 O 2 Photothermal Immunoassay... S-6 RESULTS AND DISCUSSION...S-6 TEM Characterization of Formation of the Sandwich-Type Immunoassay...S-6 REFERENCES...S-7 S-2

3 EXPERIMENTAL SECTION Materials and Instruments. We purchased 3,3,5,5 -tetramethylbenzidine (TMB), prostate-specific antigen (PSA), horseradish peroxidase (HRP), bovine serum albumin (BSA), and serum from normal human (male AB plasma) from Sigma-Aldrich (Burlington, MA, USA). Iron oxide NPs (Carboxyl-functionalized; 30 nm in diameter) were obtained from Ocean NanoTech (San Diego, CA, USA). Hepatitis B surface antigen (HBsAg) was purchased from Fitzgerald Industries International (Acton, MA, USA). Carcino-embryonic antigen (CEA), monoclonal mouse anti-human PSA antibody and polyclonal rabbit anti-human PSA antibody were obtained from Abcam (Cambridge, MA, USA). The measurement of UV-Vis absorption spectra was carried out on a SPECTROstar Nano Microplate Reader (BMG LABTECH, Cary, NC, USA) utilizing a 96-well microplate. A pen-style digital thermometer (model number KT-300 LCD) with a detection range of -50 to +300 o C was obtained from a local supermarket. A diode laser with output power from 0 to 2.5 W was purchased from Opto Engine (Midvale, UT, USA; model number MDL-III-808; wavelength 808 nm; ~7 x 17 x 5 cm in dimension). Preparation of Antibody-Conjugated Iron Oxide NPs. Antibody-conjugated iron oxide nanoparticles (NPs) were prepared using the carbodiimide method according to the literature. 1 Briefly, 1.0 mg iron oxide NPs were suspended in 2.0 ml deionized water under ultrasonication, followed by the addition of 25.0 µl aqueous mixture of S-3

4 N-hydroxysulfosuccinimide (25 mg ml -1 ) and 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (25 mg ml -1 ) for further incubation at room temperature for 30 min. Afterwards, 80.0 µg polyclonal rabbit antihuman PSA antibody was added into the nanoparticle suspension under gentle stirring, followed by further reactions at room temperature for 2.0 h. To collect the antibody-conjugated nanoparticles, the nanoparticle suspension was centrifuged at rpm for 10 min at 4.0 o C. The obtained precipitates were washed with phosphate-buffered saline (PBS; ph = 7.4, 0.01 M) 3 times. Finally, the as-prepared antibody-conjugated iron oxide NPs were suspended in 2.0 ml PBS containing 0.2% BSA. Photothermal Characterization of the Iron Oxide NPs-Mediated TMB-H 2 O 2 Colorimetric System. To monitor the photothermal effect of the Fe 3 O 4 NPs-mediated TMB-H 2 O 2 colorimetric reaction solutions (without immunoassays involved), different concentrations of Fe 3 O 4 NPs were dispersed in 0.15 ml phosphate-citrate buffer solutions (0.2 M, ph = 5.0) containing TMB (0.4 mm) and H 2 O 2 (1.0 M) in a 96-well microplate. After incubation for 40 min, the reaction solutions (0.15 ml) were transferred into PCR tubes (0.2 ml), which were then irradiated vertically with an 808 nm laser for different amounts of time (10-60 s) at a power density of 5.26 W cm -2. A pen-style digital thermometer probe was immediately inserted into the solutions to monitor the temperature after the irradiation. The highest steady vachieved after insertion of the thermometer probe for about 5-8 s was recorded as the photothermal measurement signal. S-4

5 To monitor the photothermal process of the Fe 3 O 4 NPs-mediated TMB-H 2 O 2 colorimetric reaction solutions, the colorimetric reaction solutions (1.0 ml, mg ml -1 Fe 3 O 4 NPs) were then transferred into glass cuvettes (1.5 ml), which were then irradiated horizontally with the laser for 10 min at a power density of 3.12 W cm -2. A pen-style digital thermometer was inserted into the solutions to monitor the temperature during the irradiation process. Iron Oxide NPs-Mediated TMB-H 2 O 2 Colorimetric Immunoassay. A 120 µl monoclonal mouse anti-human PSA antibody solution (30.0 µg ml -1 ) was pipetted into each polymerase chain reaction (PCR) tube (200 µl), followed by incubation at 4.0 o C overnight. A 200 µl blocking buffer solution containing 5.0% BSA was then used to block each PCR tube. Afterwards, 120 µl normal human serum (3-fold diluted with phosphate-buffered saline) spiked with different concentrations of standard PSA was pipetted into each PCR tube for further incubation at 37.5 o C for 2.0 h. After thorough washing of the PCR tubes, a 120 µl polyclonal anti-psa antibody-conjugated iron oxide NPs dispersion (0.5 mg ml -1 ) was pipetted into each PCR tube for further incubation for 2.0 h at 37.5 o C. The PCR tubes were finally thoroughly washed with phosphate-buffered saline to conduct the Fe 3 O 4 NPs-mediated TMB-H 2 O 2 colorimetric immunoassay described below. To conduct the colorimetric immunoassay, a 150 µl phosphate-citrate buffer solution (0.2 M, ph = 5.0) containing TMB (0.4 mm) and H 2 O 2 (1.0 M) was added in each PCR tube, followed by incubation at room temperature for 40 min. The solutions S-5

6 were thoroughly mixed every 5 min during the incubation period. After incubation for 40 min, the colorimetric reaction solutions were used for photograph collection, UV-Vis absorption spectroscopic characterization, and the photothermal immunoassay. Specificity of the Iron Oxide NPs-Mediated TMB-H 2 O 2 Photothermal Immunoassay. To study the specificity of the developed Fe 3 O 4 NPs-mediated TMB-H 2 O 2 photothermal immunoassay, different interfering substances, including BSA, immunoglobulin G (IgG), HBsAg, and CEA were measured using both the Fe 3 O 4 NPs-mediated TMB-H 2 O 2 colorimetric immunoassay and the photothermal immunoassay. For this purpose of validation, normal human serum samples (3-fold diluted with PBS) spiked with BSA (320 ng ml -1 ), IgG (320 ng ml -1 ), HBsAg (320 ng ml -1 ), CEA (320 ng ml -1 ), and PSA (32.0 ng ml -1 ) were tested with the colorimetric, UV-Vis absorption spectroscopic, and photothermal immunoassays, respectively. RESULTS AND DISCUSSION TEM Characterization of Formation of the Sandwich-Type Immunoassay. Transmission electron microscopy (TEM) was used to characterize iron oxide NPs captured in the sandwich-type immunoassay system, as shown in Figure S-1. After thorough washing, spherical iron oxide NPs were still observed at the substrate surface by TEM after completing the sandwich-type immunoreactions (Figure S-1B), S-6

7 with consistent morphology with that from the manufacturer (Ocean NanoTech, San Diego, CA, USA; Figure S-1A), indirectly indicating the successful completion of the sandwich-type immunoassay and the successful conjugation of antibody onto iron oxide NPs. Otherwise, iron oxide NPs would have been washed away and not imaged by TEM anymore. Figure S-1. (A) Transmission electron microscopic images of iron oxide NPs (from Ocean NanoTech) before antibody conjugation and (B) after the completion of sandwich-type immunoreactions. References 1. Jaetao, J. E.; Butler, K. S.; Adolphi, N. L.; Lovato, D. M.; Bryant, H. C.; Rabinowitz, I.; Winter, S. S.; Tessier, T. E.; Hathaway, H. J.; Bergemann, C.; Flynn, E. R.; Larson, R. S. Enhanced leukemia cell detection using a novel magnetic needle and nanoparticles. Cancer Res. 2009, 69, S-7

Dynamic light scattering (DLS)-based immunoassay for. ultrasensitive detection of tumor marker protein

Dynamic light scattering (DLS)-based immunoassay for. ultrasensitive detection of tumor marker protein Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2016 Dynamic light scattering (DLS)-based immunoassay for ultrasensitive detection of

More information

A dual-readout chemiluminescent gold lateral flow test for multiplex. and ultrasensitive detection of disease biomarkers in real samples

A dual-readout chemiluminescent gold lateral flow test for multiplex. and ultrasensitive detection of disease biomarkers in real samples Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Supporting Information for A dual-readout chemiluminescent gold lateral flow test for multiplex

More information

PRODUCT DATA SHEET. Carboxylated Fluorescent Gold Nanoparticles. Description. Characteristics

PRODUCT DATA SHEET. Carboxylated Fluorescent Gold Nanoparticles. Description. Characteristics PRODUCT DATA SHEET Carboxylated Fluorescent Gold Nanoparticles Description Cytodiagnostics carboxylated fluorescent gold nanoparticles is a unique product that combines our Cyto fluorescent dyes and gold

More information

Supplementary Information. Facile fabrication of microsphere-polymer brush hierarchically. three-dimensional (3D) substrates for immunoassays

Supplementary Information. Facile fabrication of microsphere-polymer brush hierarchically. three-dimensional (3D) substrates for immunoassays Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Supplementary Information Facile fabrication of microsphere-polymer brush hierarchically three-dimensional

More information

Supplementary Information

Supplementary Information Supplementary Information A Sensitive and Reliable Detection of Thrombin via Enzyme-Precipitate- Coating-Linked Aptamer Assay Hye-Jin Lee a, Byoung Chan Kim b, Min-Kyu Oh a,*, Jungbae Kim a,* a Department

More information

Plasmonic-driven Thermal Sensing: Ultralow Detection of Cancer Markers. Supporting Information

Plasmonic-driven Thermal Sensing: Ultralow Detection of Cancer Markers. Supporting Information Plasmonic-driven Thermal Sensing: Ultralow Detection of Cancer Markers Ester Polo, a, Pablo del Pino, a, Beatriz Pelaz, a,± Valeria Grazu, a and Jesús M. de la Fuente*,a a Instituto de Nanociencia de Aragon

More information

SCIENTIFIC REPORT. regarding the implementation of the project between December December 2014

SCIENTIFIC REPORT. regarding the implementation of the project between December December 2014 SCIENTIFIC REPORT regarding the implementation of the project between December 013 - December 014 Title of the project: DETECTION AND IDENTIFICATION OF BIOMOLECULES OF MEDICAL INTEREST BY USING MAGNETIC

More information

Manual. Catalog number:

Manual. Catalog number: LLAMA IGG ELISA Quantitation Kit Manual Catalog number: 40-374-130033 Immunoperoxidase Assay for Determination of Llama IgG in Llama serum/plasma. This kit is for research use only, and is not for use

More information

HUMAN MYELOPEROXIDASE (MPO) ELISA KIT

HUMAN MYELOPEROXIDASE (MPO) ELISA KIT HUMAN MYELOPEROXIDASE ELISA KIT Page 1 HUMAN MYELOPEROXIDASE (MPO) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN MYOELOPEROXIDASE CONCENTRATIONS IN CELL CULTURE SUPERNATES, EDTA PLASMA AND SERUM.

More information

PRODUCT DATA SHEET. Carboxylated Gold Nanoparticles. Description. Features. Storage. Applications. Handling. Characteristics

PRODUCT DATA SHEET. Carboxylated Gold Nanoparticles. Description. Features. Storage. Applications. Handling. Characteristics PRODUCT DATA SHEET Carboxylated Gold Nanoparticles Description Cytodiagnostics carboxylated gold nanoparticles are available with two different lengths of PEG surface spacers, i.e. 3000Da and 5000Da offering

More information

Hepatitis B virus Pres1 Antigen ELISA Kit

Hepatitis B virus Pres1 Antigen ELISA Kit Hepatitis B virus Pres1 Antigen ELISA Kit Catalog Number KA2118 96 assays Version: 01 Intended for research use only www.abnova.com Introduction and Background A. Test Principle This kit was based on standard

More information

Human IgG Antigen ELISA Kit

Human IgG Antigen ELISA Kit Human IgG Antigen ELISA Kit Catalog No: IHUIGGKT Lot No: SAMPLE INTENDED USE This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum,

More information

AssayMax Mouse Transferrin ELISA Kit

AssayMax Mouse Transferrin ELISA Kit AssayMax Mouse Transferrin ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Mouse OVA IgG1 ELISA. Catalog Number M046072

Mouse OVA IgG1 ELISA. Catalog Number M046072 Mouse OVA IgG1 ELISA Catalog Number M046072 For the quantitative determination of Ovalbumin specific IgG1 in mouse plasma, serum, tissue and cell culture supernate samples. For research use only. This

More information

Mouse Axl ELISA Pair Set

Mouse Axl ELISA Pair Set Mouse Axl ELISA Pair Set Catalog Number : SEK50126 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

Mouse IFNAR1 ELISA Pair Set

Mouse IFNAR1 ELISA Pair Set Mouse IFNAR1 ELISA Pair Set Catalog Number : SEK50469 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

Human BMP-2 ELISA Pair Set

Human BMP-2 ELISA Pair Set Human BMP-2 ELISA Pair Set Catalog Number : SEK10426 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

PACKAGE INSERT Applied BioCode, Inc. - Beads. CARBOXYL BARCODED MAGNETIC BEADS (BMBs) 128-Plex - Part Number 44-B Plex - Part Number 44-B0302

PACKAGE INSERT Applied BioCode, Inc. - Beads. CARBOXYL BARCODED MAGNETIC BEADS (BMBs) 128-Plex - Part Number 44-B Plex - Part Number 44-B0302 For Coupling Nucleic Acids: PACKAGE INSERT Applied BioCode, Inc. - Beads CARBOXYL BARCODED MAGNETIC BEADS (BMBs) 128-Plex - Part Number 44-B0102 4096-Plex - Part Number 44-B0302 For Coupling Proteins:

More information

Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set

Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set Human Junctional Adhesion Molecule A / JAM-A ELISA Pair Set Catalog Number : SEKA10198 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run

More information

Human Serum Albumin (HSA) ELISA Quantitation Kit. Manual

Human Serum Albumin (HSA) ELISA Quantitation Kit. Manual Human Serum Albumin (HSA) ELISA Quantitation Kit Manual Catalog number: 40-288-10067F For the quantitative determination of human serum albumin levels in plasma or other biological samples. This kit is

More information

Human Transferrin / TF ELISA Pair Set

Human Transferrin / TF ELISA Pair Set Human Transferrin / TF ELISA Pair Set Catalog Number : SEK11019 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the

More information

Anti-Asian Sea bass (Lates calcarifer) IgM monoclonal antibody labelled with horseradish peroxidase. Product no: C2-HRP

Anti-Asian Sea bass (Lates calcarifer) IgM monoclonal antibody labelled with horseradish peroxidase. Product no: C2-HRP Anti-Asian Sea bass (Lates calcarifer) IgM monoclonal antibody labelled with horseradish peroxidase Product no: C2-HRP Product Description This monoclonal antibody (Mab) reacts with Asian Sea bass (Lates

More information

HUMAN SOLUBLE NEUROPILIN 2 (snrp2) ELISA KIT

HUMAN SOLUBLE NEUROPILIN 2 (snrp2) ELISA KIT Page 1 HUMAN SOLUBLE NEUROPILIN 2 (snrp2) ELISA KIT PURCHASE INFORMATION: FOR THE QUANTITATIVE DETERMINATION OF HUMAN SOLUBLE NEUROPILIN 2 CONCENTRATIONS IN PLASMA AND SERUM. ELISA NAME Catalog No. Lot

More information

Human C-Reactive Protein / CRP ELISA Pair Set

Human C-Reactive Protein / CRP ELISA Pair Set Human C-Reactive Protein / CRP ELISA Pair Set Catalog Number : SEK11250 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized

More information

Mouse CD34 ELISA Pair Set

Mouse CD34 ELISA Pair Set Mouse CD34 ELISA Pair Set Catalog Number : SEK50589 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

RayBio Mouse Insulin ELISA Kit

RayBio Mouse Insulin ELISA Kit RayBio Mouse Insulin ELISA Kit Catalog #: ELM-Insulin User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

Human IL10RB ELISA Pair Set ( CRFB4 )

Human IL10RB ELISA Pair Set ( CRFB4 ) Human IL10RB ELISA Pair Set ( CRFB4 ) Catalog Number : SEK10945 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the

More information

Cyno Monkey IgG Antigen ELISA Kit

Cyno Monkey IgG Antigen ELISA Kit Cyno Monkey IgG Antigen ELISA Kit Catalog No: ICYIGGKT Lot No: SAMPLE INTENDED USE This cynomolgus macaque (Macaca fascicularis) monkey Immunoglobulin G (IgG) antigen assay is intended for the quantitative

More information

Mouse TNF-α ELISA MAX Set Deluxe

Mouse TNF-α ELISA MAX Set Deluxe Mouse TNF-α ELISA MAX Set Deluxe Cat. No. 430904 (5 plates) 430905 (10 plates) 430906 (20 plates) ELISA Set for Accurate Cytokine Quantification from Cell Culture Supernatant, Serum, Plasma or Other Body

More information

TNF (Pig) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only.

TNF (Pig) ELISA Kit. Catalog Number KA assays Version: 05. Intended for research use only. TNF (Pig) ELISA Kit Catalog Number KA1823 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3 General Information...

More information

Human IGFBP7 ELISA Pair Set

Human IGFBP7 ELISA Pair Set Human IGFBP7 ELISA Pair Set Catalog Number : SEK13100 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

Human SLAMF6 / Ly108 ELISA Pair Set

Human SLAMF6 / Ly108 ELISA Pair Set Human SLAMF6 / Ly108 ELISA Pair Set Catalog Number : SEK11945 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General

More information

AssayMax Human IgD ELISA Kit

AssayMax Human IgD ELISA Kit AssayMax Human IgD ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing the

More information

AssayMax Human Cystatin-A (CSTA) ELISA Kit

AssayMax Human Cystatin-A (CSTA) ELISA Kit AssayMax Human Cystatin-A (CSTA) ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or

More information

Human soluble TREM-2 ELISA Kit

Human soluble TREM-2 ELISA Kit Human soluble TREM-2 ELISA Kit Cat.No: DEIA-SY005 Lot. No. (See product label) Size 96T Intended use For the quantitative measurement of human soluble TREM-2 concentrations in serum, plasma. General Description

More information

Human Basic Fibroblast growth factor (bfgf) ELISA

Human Basic Fibroblast growth factor (bfgf) ELISA Human Basic Fibroblast growth factor (bfgf) ELISA For the quantitative determination of human bfgf in serum, plasma, cell culture supernatants, body fluid and tissue homogenate Cat. No. KT-52774 For Research

More information

Human Soluble LDLR ELISA Kit

Human Soluble LDLR ELISA Kit Human Soluble LDLR ELISA Kit Cat.No: DEIA-XY2221 Lot. No. (See product label) Size 96T Intended Use This Human Soluble LDLR ELISA Kit contains the necessary components required for the quantitative measurement

More information

Armenian Hamster IgG Antigen ELISA Kit

Armenian Hamster IgG Antigen ELISA Kit Armenian Hamster IgG Antigen ELISA Kit Catalog No: IAHTIGGKT Lot No: SAMPLE INTENDED USE This Armenian hamster Immunoglobulin G (IgG) antigen assay is intended for the quantitative determination of total

More information

Rhesus CD16 / FCGR3 ELISA Pair Set

Rhesus CD16 / FCGR3 ELISA Pair Set Rhesus CD16 / FCGR3 ELISA Pair Set Catalog Number : SEK90013 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General

More information

MULTISPECIES LBP Quantification ELISA

MULTISPECIES LBP Quantification ELISA MULTISPECIES LBP Quantification ELISA For the quantitative determination of natural and recombinant human, bovine, pig, rabbit, dog LBP in serum, plasma and culture medium Cat. No. KT-1005 For Research

More information

For the quantitative measurement of Human IgM concentrations in cell culture supernatants, plasma, serum, saliva, urine and milk.

For the quantitative measurement of Human IgM concentrations in cell culture supernatants, plasma, serum, saliva, urine and milk. ab137982 IgM Human ELISA Kit Instructions for Use For the quantitative measurement of Human IgM concentrations in cell culture supernatants, plasma, serum, saliva, urine and milk. This product is for research

More information

Mouse Anti-SRBC IgG ELISA Kit

Mouse Anti-SRBC IgG ELISA Kit Mouse Anti-SRBC IgG ELISA Kit CATALOG NO: IRKTAH3033 LOT NO: SAMPLE INTENDED USE The mouse anti-srbc IgG test kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses detergent

More information

CytoGLOW. IKK-α/β. Colorimetric Cell-Based ELISA Kit. Catalog #: CB5358

CytoGLOW. IKK-α/β. Colorimetric Cell-Based ELISA Kit. Catalog #: CB5358 CytoGLOW IKK-α/β Colorimetric Cell-Based ELISA Kit Catalog #: CB5358 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only.

More information

ELISA MAX Set Deluxe. Human IL-32α

ELISA MAX Set Deluxe. Human IL-32α ELISA MAX Set Deluxe Human IL-32α Cat. No. 433504 (5 plates) 433505 (10 plates) ELISA Set for Accurate Cytokine Quantitation from Cell Culture Supernatant, Serum, Plasma or Other Body Fluids BioLegend,

More information

Human AFP / alpha-fetoprotein ELISA Pair Set

Human AFP / alpha-fetoprotein ELISA Pair Set Human AFP / alpha-fetoprotein ELISA Pair Set Catalog Number : SEK12177 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized

More information

Human IL-10 ELISA MAX Set Deluxe

Human IL-10 ELISA MAX Set Deluxe Human IL-10 ELISA MAX Set Deluxe Cat. No. 430604 (5 plates) 430605 (10 plates) 430606 (20 plates) ELISA Set for Accurate Cytokine Quantification from Cell Culture Supernatant, Serum, Plasma or Other Body

More information

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests Human IgG ELISA Kit Strip well format. Reagents for up to 96 tests Catalog No. CS222A Quantity: 1 x 96 tests CS222B 5 x 96 tests Intended Use: Background: Assay Principle: This human immunoglobulin G antigen

More information

AssayMax Human Transferrin ELISA Kit

AssayMax Human Transferrin ELISA Kit AssayMax Human Transferrin ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

RayBio Phospho- Stat 3 (Tyr705) ELISA Kit

RayBio Phospho- Stat 3 (Tyr705) ELISA Kit RayBio Phospho- Stat 3 (Tyr705) ELISA Kit For Measuring Phosphorylated Stat3 (Tyr705) in Human, Mouse and Rat Cell Lysates User Manual (Revised Mar 1, 2012) RayBio Stat3 (Tyr705) ELISA Kit Protocol (Cat#:

More information

Human Anti-platelet antibody ELISA kit

Human Anti-platelet antibody ELISA kit Human Anti-platelet antibody ELISA kit Cat.No: DEIA-BJ111 Lot. No. (See product label) Size 96T Intended Use This CD Anti-platelet antibody ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative

More information

EGFR (Phospho-Ser695)

EGFR (Phospho-Ser695) Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02090 Please read the provided manual entirely

More information

Human PTH / PTH1 / Parathyroid Hormone ELISA Pair Set

Human PTH / PTH1 / Parathyroid Hormone ELISA Pair Set Human PTH / PTH1 / Parathyroid Hormone ELISA Pair Set Catalog Number : SEK13192 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized

More information

RayBio Human Hydroxylated-HIF-1alpha (Pro402) and HIF-1alpha ELISA Kit

RayBio Human Hydroxylated-HIF-1alpha (Pro402) and HIF-1alpha ELISA Kit RayBio Human Hydroxylated-HIF-1alpha (Pro402) and HIF-1alpha ELISA Kit For Measuring Hydroxylated HIF-1alpha (Pro402) and HIF-1alpha in Human Cell Lysates User Manual (Revised Aug 26 th, 2016) RayBio Hydroxylated-HIF-1alpha

More information

Mouse Neurofilament light polypeptide ELISA kit

Mouse Neurofilament light polypeptide ELISA kit Mouse Neurofilament light polypeptide ELISA kit Cat.No: DEIABL-M1 Lot. No. (See product label) Size 96T Intended use This CD Neurofilament light polypeptide ELISA kit is a 1.5 hour solid-phase ELISA designed

More information

Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set

Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set Human TNF-alpha / TNFA / TNFSF2 ELISA Pair Set Catalog Number : SEKA10602 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized

More information

Cancer Antigen CA125 Human ELISA Kit

Cancer Antigen CA125 Human ELISA Kit ab108653 Cancer Antigen CA125 Human ELISA Kit Instructions for Use For the quantitative measurement of Human Cancer Antigen CA125 concentrations in serum This product is for research use only and is not

More information

Mouse helicobacter pylori antibody (IgG) ELISA Kit

Mouse helicobacter pylori antibody (IgG) ELISA Kit Mouse helicobacter pylori antibody (IgG) ELISA Kit Catalog Number. CSB-E14132m For the qualitative determination of mouse helicobacter pylori antibody (IgG) concentrations in serum, plasma. This package

More information

Bovine Serum Albumin (BSA) Assay

Bovine Serum Albumin (BSA) Assay Bovine Serum Albumin (BSA) Assay Immunoenzymetric Assay for the Measurement of BSA Catalog # F030 Intended Use This kit is intended for use in quantitating bovine serum albumin (BSA). The kit is for Research

More information

Supplementary information

Supplementary information Supplementary information Vertical flow immunoassay (VFA) biosensor for a rapid one step immunoassay Young Kyoung Oh, a Hyou-Arm Joung, b Sanghyo Kim c and Min-Gon Kim* a,b a Advanced Photonics Research

More information

SRBC IgG (Mouse) ELISA Kit

SRBC IgG (Mouse) ELISA Kit SRBC IgG (Mouse) ELISA Kit Catalog Number KA2462 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the

More information

PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair

PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair Pack Size: 96 tests / 480 tests Catalog Number:EP-101 IMPORTANT: Please carefully read this manual before performing your experiment. For

More information

Human ECM1 ELISA Pair Set

Human ECM1 ELISA Pair Set Human ECM1 ELISA Pair Set Catalog Number : SEK10362 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

Nanoscale. Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer COMMUNICATION

Nanoscale. Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer COMMUNICATION COMMUNICATION Cite this: Nanoscale, 2016, 8, 5422 Received 20th December 2015, Accepted 18th January 2016 DOI: 10.1039/c5nr09051b www.rsc.org/nanoscale Nanoparticle-mediated photothermal effect enables

More information

SensoLyte Anti-alpha-Synuclein Quantitative ELISA Kit (Rat) *Colorimetric*

SensoLyte Anti-alpha-Synuclein Quantitative ELISA Kit (Rat) *Colorimetric* SensoLyte Anti-alpha-Synuclein Quantitative ELISA Kit (Rat) *Colorimetric* Revision number: 1.3 Last updated: 1/15/18 Catalog # AS-55550-R Kit Size One 96-well strip plate This kit is optimized to detect

More information

Mouse SerpinF2 ELISA Pair Set

Mouse SerpinF2 ELISA Pair Set Mouse SerpinF2 ELISA Pair Set Catalog Number : SEK50167 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General

More information

ELISA PRODUCT INFORMATION & MANUAL

ELISA PRODUCT INFORMATION & MANUAL ELISA PRODUCT INFORMATION & MANUAL Human Transthyretin/ Prealbumin ELISA Kit NBP2-60516 Enzyme-linked Immunosorbent Assay for quantitative detection of Human Prealbumin. For research use only. Not for

More information

RayBio Mouse IgE ELISA Kit

RayBio Mouse IgE ELISA Kit RayBio Mouse IgE ELISA Kit Cat#: ELM-IgE User Manual Last revised June 14, 2014 Please read the entire manual carefully before starting your experiment RayBiotech, Inc. ISO 13485 Certified Tel: 1-888-494-8555

More information

HUMAN LBP Quantification ELISA

HUMAN LBP Quantification ELISA HUMAN LBP Quantification ELISA For the quantitative determination of natural and recombinant human LBP in serum, plasma and culture medium Cat. No. KT-1004 For Research Use Only. Not for use in diagnostic

More information

Human ICAM-2 / CD102 ELISA Pair Set

Human ICAM-2 / CD102 ELISA Pair Set Human ICAM-2 / CD102 ELISA Pair Set Catalog Number : SEK10332 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General

More information

AssayMax Human IL-6 ELISA Kit

AssayMax Human IL-6 ELISA Kit AssayMax Human IL-6 ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing the

More information

RayBio Human Caspase-3 ELISA Kit

RayBio Human Caspase-3 ELISA Kit RayBio Human Caspase-3 ELISA Kit Catalog #: ELH-CASP3 User Manual Last revised July 21, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

NAG-1 (GDF-15, MIC-1) Prostate Cancer ELISA kit

NAG-1 (GDF-15, MIC-1) Prostate Cancer ELISA kit NAG-1 (GDF-15, MIC-1) Prostate Cancer ELISA kit Catalog Number: NG1 Store at -0 C. FOR RESEARCH USE ONLY v. 1081 Introduction This sandwich ELISA kit is for determination of NAG-1 (GDF-15, MIC-1) levels

More information

Human IGFBP3 ELISA Pair Set

Human IGFBP3 ELISA Pair Set Human IGFBP3 ELISA Pair Set Catalog Number : SEK10430 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

Human 25 Hydroxy Vitamin D3 ELISA kit

Human 25 Hydroxy Vitamin D3 ELISA kit Human 25 Hydroxy Vitamin D3 ELISA kit Cat.No: DEIA-BJ648 Lot. No. (See product label) Size 96T Intended use This CD 25 Hydroxy Vitamin D3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative

More information

BoLISA BoNT Sandwich ELISA Protocol

BoLISA BoNT Sandwich ELISA Protocol BoLISA BoNT Sandwich ELISA Protocol 55 S. Rosa Road, Suite 5 Madison, WI 5379-68-44-874 info@biosentinelpharma.com BioSentinel Part No: L7, Release Date: May, 7 BoLISA A BoNT/A Sandwich ELISA Detection

More information

INOS. Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG00807

INOS. Colorimetric Cell-Based ELISA Kit. Catalog #: OKAG00807 INOS Colorimetric Cell-Based ELISA Kit Catalog #: OKAG00807 Please read the provided manual entirely prior to use as suggested experimental protocols may have changed. Research Purposes Only. Not Intended

More information

Ferritin Human ELISA Kit

Ferritin Human ELISA Kit ab108837 Ferritin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Ferritin concentrations in cell culture supernatants, milk, serum and plasma This product is for research

More information

RayBio Human and Mouse Cleaved-Caspase-7 (Asp198) and Caspase-7 ELISA Kit

RayBio Human and Mouse Cleaved-Caspase-7 (Asp198) and Caspase-7 ELISA Kit RayBio Human and Mouse Cleaved-Caspase-7 (Asp198) and Caspase-7 ELISA Kit For Measuring Cleaved Caspase-7 (Asp198) and Caspase-7 in Human and Mouse Cell Lysates User Manual (Revised Aug 26 th, 2016) RayBio

More information

Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit. MyBioSource.com

Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit. MyBioSource.com Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit For the qualitative determination of human glutamic acid decarboxylase 65(GAD65) antibody (IgG) concentrations in serum. This package

More information

Rat Cardiac Troponin T (ctnt) ELISA

Rat Cardiac Troponin T (ctnt) ELISA Rat Cardiac Troponin T (ctnt) ELISA For the quantitative determination of rat ctnt in serum, plasma, cell culture supernates, tissue homogenates and other biological fluids Cat. No. KT-60845 For Research

More information

Human ADAM15 ELISA Pair Set

Human ADAM15 ELISA Pair Set Human ADAM15 ELISA Pair Set Catalog Number : SEK10517 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in the General ELISA

More information

RayBio Human IgG1 ELISA Kit

RayBio Human IgG1 ELISA Kit RayBio Human IgG1 ELISA Kit Catalog #: ELH-IGG1 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA

More information

HUMAN TRANSFORMING GROWTH FACTOR BETA 1 (TGF- 2) ELISA KIT

HUMAN TRANSFORMING GROWTH FACTOR BETA 1 (TGF- 2) ELISA KIT P a g e 1 HUMAN TRANSFORMING GROWTH FACTOR BETA 1 (TGF- 2) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN TGF- 2 CONCENTRATIONS IN CELL CULTURE SUPERNATES, SERUM, AND PLASMA. Purchase Information:

More information

MyBioSource.com. Rat 25 Hydroxy Vitamin D3 (25-OHVD3) Elisa kit (Sandwich ELISA)

MyBioSource.com. Rat 25 Hydroxy Vitamin D3 (25-OHVD3) Elisa kit (Sandwich ELISA) Rat 25 Hydroxy Vitamin D3 (25-OHVD3) Elisa kit (Sandwich ELISA) 96 Tests Catalog Number: MBS720917 Store all reagents at 2-8 C Valid Period: six months For samples: Serum, plasma, cell culture supernatants,

More information

RayBio Phospho- Akt (Ser473) ELISA Kit

RayBio Phospho- Akt (Ser473) ELISA Kit RayBio Phospho- Akt (Ser473) ELISA Kit For Measuring Phosphorylated Akt (Ser473) in Human, Mouse and Rat Cell Lysates User Manual (Revised Mar 1, 2012) RayBio Akt (Ser473) ELISA Kit Protocol (Cat#: PEL-Akt-S473-001)

More information

Mouse hepatitis E virus (HEV) antibody (IgG) ELISA Kit

Mouse hepatitis E virus (HEV) antibody (IgG) ELISA Kit Mouse hepatitis E virus (HEV) antibody (IgG) ELISA Kit For the qualitative determination of mouse hepatitis E virus (HEV) antibody (IgG) concentrations in serum. This package insert must be read in its

More information

Mouse Coagulation Factor X (F10) ELISA

Mouse Coagulation Factor X (F10) ELISA Mouse Coagulation Factor X (F10) ELISA For the quantitative determination of mouse F10 in biological samples. Cat. No. KT-52133 For Research Use Only. Not for use in diagnostic procedures. Page 1 of 5

More information

Mouse Sclerostin ELISA

Mouse Sclerostin ELISA Mouse Sclerostin ELISA For the quantitative determination of sclerostin in mouse serum and plasma. For Research use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 41-SCLMS-E01 96 Wells

More information

MyBioSource.com. Human α Melanocyte Stimulating. Hormone (αmsh) Elisa kit (Sandwich ELISA)

MyBioSource.com. Human α Melanocyte Stimulating. Hormone (αmsh) Elisa kit (Sandwich ELISA) Human α Melanocyte Stimulating Hormone (αmsh) Elisa kit (Sandwich ELISA) 96 Tests Catalog Number: MBS724629 Store all reagents at 2-8 C Valid Period: six months For samples: Serum, plasma, cell culture

More information

For the quantitative measurement of human, mouse and rat phosphorylated STAT3 (Tyr705) concentrations in cell lysates.

For the quantitative measurement of human, mouse and rat phosphorylated STAT3 (Tyr705) concentrations in cell lysates. ab126458 - STAT3 (py705) ELISA Kit Instructions for Use For the quantitative measurement of human, mouse and rat phosphorylated STAT3 (Tyr705) concentrations in cell lysates. This product is for research

More information

MyBioSource.com. Rat 1,25 Hydroxy Vitamin D3 (1,25OHVD3) Elisa kit(sandwich ELISA)

MyBioSource.com. Rat 1,25 Hydroxy Vitamin D3 (1,25OHVD3) Elisa kit(sandwich ELISA) Rat 1,25 Hydroxy Vitamin D3 (1,25OHVD3) Elisa kit(sandwich ELISA) 96 Tests Catalog Number: MBS733576 Store all reagents at 2-8 C Valid Period: six months For samples: Serum, plasma, cell culture supernatants,

More information

Fecal Helicobacter pylori-antigen ELISA

Fecal Helicobacter pylori-antigen ELISA Instructions for Use Fecal Helicobacter pylori-antigen ELISA Enzyme immunoassay for the quantitative and qualitative determination of Helicobacter pylori antigen in feces. RE58891 96 2-8 C I B L I N T

More information

Human Peptide YY EIA

Human Peptide YY EIA KAMIYA BIOMEDICAL COMPANY Human Peptide YY EIA For the quantitative determination of Peptide YY in human serum and plasma. Cat. No. KT-378 For Research Use Only. 1 Rev. 9822378 PRODUCT INFORMATION Human

More information

Electronic Supplementary Information. Enzyme-free catalytic DNA circuit for amplified detection of aflatoxin B1 using

Electronic Supplementary Information. Enzyme-free catalytic DNA circuit for amplified detection of aflatoxin B1 using Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2016 Electronic Supplementary Information Enzyme-free catalytic DNA circuit for amplified detection

More information