Selection of microalgae from native strains in Suriname and process development for optimal lipid productivity

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emphasis in Bioprocesstechnology Institute for

1 Selection of microalgae from native strains in Suriname and process development for optimal lipid productivity Prof. dr. R. Mohan Academic Chair: Biotechnology with specific emphasis in Bioprocesstechnology Institute for Graduate Studies and Research-Anton de Kom Universiteit van Suriname (IGSR/AdeKUS) 1

2 Table of content Introduction Statement of the problem Research goal Overall objective Specific objective Justification Intermediate results Possible tryouts Further planning Source: 2

3 Introduction Microalgae in general Freshwater and marine species Fast growers (20-30 times faster than traditional food crops) All algal species produce Proteins, Carbohydrates, Fats and Nucleic Acids (varying proportions) Possibility of product accumulate with environmental changes (e.g. temperature, illumination, ph, CO2 supply) Over 15,000 novel compounds originating from algal biomass have been chemically determined The fatty acid (oil) can be extracted and converted into biodiesel 3/13/2013 3

4 Introduction Possible applications of microalgae Species Chlorella vulgaris Dunaliella salina Arthrospira maxima Chlorella vulgaris Rhodella sp. Skeletonema sp. Neochloris oleabundans Application Source of natural oxidants Produce caretenoids High protein content/nutritional supplement Colorant for cosmetics Aquaculture Suitable renewable lipid source

5 Statement of the problem Research proved that biofuels can be generated from algal oil (lipids) Research proved that algae produce times more oil than any other oil crop Most feasibility studies have been done in Europe and Northern America (with native species) For the tropical countries it is still the challenge to find out the suitable techniques and strains 3/13/2013 5

microalgae species Source: www.biotechnology.

6 Research goal Explore techniques to enhance lipid and protein production in selected native microalgae species Source: Source: 3/13/2013 6

taking into account the logistics for supply of nutrients

7 Overall objective Design a production system and analyze the economical and technological feasibility for Suriname, taking into account the logistics for supply of nutrients and CO₂. Source: Source: 3/13/2013 7

production Studying a bioreactor and process conditions to develop an optimal

8 Specific objectives Screening the lipid production of microalgae strains collected from nature in Suriname Selecting the microalgae strain with the highest lipid production Studying a bioreactor and process conditions to develop an optimal process of lipid production Source: Source: 3/13/2013 8

conditions Environmental and physiological conditions can be easily simulated and dominated in

9 Why native algae? Native species are preferable, because: Already adapted to the local environment and conditions Environmental and physiological conditions can be easily simulated and dominated in laboratory Limited risk for invasive species Ideal strains can easily be improve by breeding manipulation Source: Source: 3/13/2013 9

10 Intermediate results Draft research proposal prepared Two draft review articles prepared Laboratory established and equipment arranged Basic Algal overview document prepared (WLA data) Some essential courses are attended at the home institute (writing skills, GLP and QMS, HPLC and AAS training) 3/13/

Intermediate results WLA data : Water Monitoring department of Ministry of Public Works Starting point and the incentive for this research Freshwater microalgae collected in the

11 Intermediate results WLA data : Water Monitoring department of Ministry of Public Works Starting point and the incentive for this research Freshwater microalgae collected in the inland waters in and near Paramaribo Concerns identifications on behalf of water quality management research ( , ones/month) Source: 3/13/

12 Intermediate results WLA sampling areas Area Code Year Saramacca kanaal Sk 1999 Groot Paramaribo GP4/GP5 2004/2005 Bigi-Pan BP 2004 Staatsolie -area Tambaredjo ST 2006 Para river PR Iam Golg IG /13/

13 Intermediate results Number of species found and identified by WLA Area Number of species found Saramacca kanaal 159 Groot paramaribo 101 Bigi-Pan 36 Staatsolie-area Tambaredjo 114 Para river 115 Iam Gold- Rosebel Mines 28 About 79 genera and 381 species are identified (Source: WLA, Ministerie van Openbare Werken en Verkeer) 3/13/

14 Possible tryouts 9 genera Based on literature study and available information about lipid, protein content and biomass production Native species should be tryout, because no detailed information is available about our native species! Source: 3/13/

15 Possible tryouts Genus Information about Where Ankistrodesmus Lipid content 28-40% WUR Cyclotella Lipid content 35-42% WUR Nitzchia Lipid content 28-50% WUR Skeletonema Lipid content 21-32% WUR Scenedesmus Lipid content % WUR Spirullina Protein content 46-71% Oilgae.com Scenedesmus Protein content 8-56% Oligae.com Spirogyra Protein content 6-20% Oligae.com Euglena Protein content 39-61% Oligae.com Anabaena Protein content 43-56% Oligae.com 3/13/

Project activity Review in Algae research (bibliographic and desktop research) Acquire and/or refreshment of basic skills to screen, grow and analyze algae Fieldwork: collection of native strains in

16 Project activity Review in Algae research (bibliographic and desktop research) Acquire and/or refreshment of basic skills to screen, grow and analyze algae Fieldwork: collection of native strains in nature Lab activities: identification, screening lipid production, characterizing growth rate and lipid profile, and selection of the two most promising strains Activities and time schedule Implementation period 01 December February 2013 ( 2 months) 01 February- 01 June 2013 (5 months) 01 June May 2014 (12 months) Responsible Applicant; PhD student; Research assistant 1; Research assistant 2. PhD student; Researchers of WUR; Research assistant 1. Applicant; Researchers of WUR; PhD student; Research assistant 1; Research assistant 2. Type of evaluation Location Review report and chapter summaries Report of elaborated operational and methodological protocols Quarter reports elaborated, review papers, Abstracts for participation in congress AdeKUS, Suriname Wageningen University and Research, Netherland AdeKUS, Suriname Specific locations for collections; Physical, chemical and bioprocess laboratories 3/13/

17 Photo-bioreactor development for laboratory experiments Optimization of cultivation conditions for continuous lipid production form selected strains Data processing (verification, validation, justification) and results communication Activities and time schedule (cont.) 01 May 2013 t/m November 2013 (6 months) 01 November June 2014 (07 months) 01 June 31 September 2014 (04 months) Applicant; Researchers of WUR; PhD student; Research assistant 1; Research assistant 2. Applicant; Researchers of WUR; PhD student; Research assistant 1; Research assistant 2. Applicant; Researchers of WUR; PhD student; Research assistant 1; Research assistant 2. Semester reports elaborated, Design layouts and descriptions, Abstracts for participation in congress Partial reports elaborated, concept papers, Abstracts for participation in congress Paper publications; Final project report; Congress participation abstracts and posters; thesis Wageningen University and Research, Netherland and AdeKUS, Suriname Wageningen University and Research, Netherland and AdeKUS, Suriname Wageningen University and Research, Netherland and AdeKUS, Suriname 3/13/

18 Thank you. Questions? 3/13/

Managing Microalgae at the Pilot Scale

Managing Microalgae at the Pilot Scale 7 th Algae Biomass Summit October 1 st, 2013 4:30 PM Schonna R. Manning, Ph.D. Research Associate Introduction Applied Phycology *water proteins lipids + CO 2 carbs