GRAM STAIN/CULTURE of CSF and OTHER BODY FLUIDS by HEMATOLOGY LAB (11:30pm 07:30am)

Size: px
Start display at page:

Download "GRAM STAIN/CULTURE of CSF and OTHER BODY FLUIDS by HEMATOLOGY LAB (11:30pm 07:30am)"

Transcription

1 Created Rebecca Riemann Clin. Lab 5/22/2012 Tech 1 Revised Eric Spitzer Chief of 5/22/2012 Micro/Assoc. Dir Labs Reviewed Jay Bock M.D. 5/22/2012 Approved Eric Spitzer Chief of Micro/Assoc. Dir Labs mic /22/2012 GRAM STAIN/CULTURE of CSF and OTHER BODY FLUIDS by HEMATOLOGY LAB (11:30pm 07:30am) I. PRINCIPLE To provide for appropriate turnaround time for processing of critical specimens, the Hematology Lab will perform Gram stain and culture on all CSF specimens received from 1130pm-0730am. In addition, the Hematology Lab will perform a Gram stain only on any body fluid received from 1130pm-0730am. (Refer to SPE 3.4) II. REAGENTS AND SUPPLIES A. Media and Reagents 1. BBL 5% sheep blood agar, chocolate agar and THIO Broth 2. BBL Gram stain reagents B. Supplies 1. Cytoslides 2. Methanol, absolute 3. Cytospin 4. Cetrifuge, vortex mixer 5. Sterile snap cap tubes (Falcon 2054), sterile Pasteur pipettes, inoculating loops III. QUALITY CONTROL (Gram Stain Reagents) 1. All new Lot number/shipments are quality controlled by the Microbiology Lab. 2. Weekly quality control will be performed by Hematology and recorded in the appropriate QC log. 3. Expected results: a. Staph aureus ATCC positive (deep violet) b. E. coli ATCC negative (pink or red) Corrective Action: If the above expected results are not obtained, request a new lot number of reagent from the Microbiology Lab. Gram stain for Hematology night shift Page 1 of 7

2 IV. PROCEDURE A. Perform all the initial processing of specimens in a biological safety cabinet (or, if not available, behind a shield). B. Gram Stain: 1. Prepare a smear using the cytospin. 2. Heat fix the smear on an electric slide warmer until dry. Add one drop of Methanol. Methanol fixation prevents lysis of RBCs, results in cleaner background and prevents wash-off of specimen from slide. Allow slide to dry and then cool before staining. 3. Flood the fixed smear with the crystal violet solution. Allow stain to remain for 30 s. 4. Decant crystal violet and rinse slide gently with running tap water (excessive rinsing can cause crystal violet to be washed from gram-positive cells). 5. Rinse off excess water with iodine solution, then flood the slide with fresh iodine solution. Allow iodine to remain for 30 s. 6. Rinse gently with flowing tap water. 7. Decolorize by letting acetone/alcohol reagent flow over smear while slide is held at an angle. Stop when runoff becomes clear. Adjust decolorization time to thickness of smear. 8. Remove excess decolorizer with gentle flow of tap water (excess washing can cause dye-iodine complex to be washed from gram-positive cells). 9. Flood slide with safranin counterstain, allow to remain for 30 s. 10. Remove excess counterstain with gentle flow of tap water. 11. Drain slide and air dry upright or use slide warmer. 12. Examine the smear microscopically using the oil immersion objective. 13. Turnaround Time: CSF = 0-60 minutes Non-CSF Body Fluids = minutes C. Culture: (Culture will only be set up on CSF) 1. If >1 ml is received, centrifuge for 20 min at x g at 20 C. Allow the centrifuge to stop. Do not brake. a. Aspirate the supernatant with a sterile pipette, leaving approximately 0.5 ml of fluid in the specimen tube. Reserve the supernatant in a sterile snap cap tube for antigen studies. b. Vortex the sediment vigorously for at least 30 s to resuspend the pellet. Do not use a pipette to mix the sediment, because the bacteria and cells may adhere to the sides of the tube and cause false-negative findings. c. Use a sterile pipette to inoculate the media by placing 1 or 2 drops of the vortexed sediment onto each plate and into the broth. 2. If ml is received, vortex, then inoculate. 3. Place media in 37 C incubator and Microbiology Lab will pick up first thing in the a.m. Gram stain for Hematology night shift Page 2 of 7

3 V. INTERPRETATION AND REPORTING OF RESULTS 1. Gram reactions Gram positive: deep violet Gram negative: pink or red 2. Evaluate smear Background should be clear or gram-negative. WBCs should be completely Gram negative. If excess precipitated stain is observed, prepare another smear and stain it with freshly filtered crystal violet or decolorize and repeat the stain. 3. Reporting results Examine several areas of smear under oil immersion for the presence of WBCs and microorganisms. a. If no organisms/wbcs are detected report No organisms seen and/or No WBCs as appropriate. b. If organisms/wbcs are observed, report relative numbers Rare (<1 per oil immersion field) Few (1 to 5 per oil immersion field) Moderate (6 to 10 per oil immersion field) Many (>10 per oil immersion field) (Refer to the Entering Gram Stain Results in Misys GUI section of this procedure.) VI. ENTERING GRAM STAIN RESULTS IN MISYS (SUNQUEST) GUI 1) Click on the Laboratory folder in Citrix. 2) Click on the Sunquest Laboratory Icon. 3) Click OK for the work area: Prod-Lab 4) Sunquest Login Screen-Enter your LIS tech code as the User, followed by your password and then change Location from R to HE. Gram stain for Hematology night shift Page 3 of 7

4 5) Double click on the Microbiology Result Entry folder. 6) The Data entry mode will default to Specimen Mode and the Data search will default to Lookup by: Accession Number. Type the accession number of the CSFC or FLDC in the Value Box and click on Search. 7) Double click on the test you want. 8) You will automatically be brought to the Direct Exam Tab and be at the Observations. Gram stain for Hematology night shift Page 4 of 7

5 9) Hit the F8 key to get the Micro Direct Exam Keyboard. 10) Click on the appropriate key on the displayed keyboard for the result you wish to enter or just hit the corresponding key for the result you want to enter. Enter the quantity followed by the result as shown in the example below. (Hitting F8 again will remove the displayed keyboard.) KEY RESULT CODE TRANSLATION 1 RARE RARE 2 FEW FEW 3 MOD MOD 4 MANY MANY Q WBCS WBCS SEEN Y RBS RBCS SEEN A GPC GRAM POSITIVE COCCI Z GNC GRAM NEGATIVE COCCI S GPR GRAM POSITIVE RODS N GNR GRAM NEGATIVE RODS P YEAS YEAST Codes not on keyboard. Use one semicolon (;) to enter the GPCP GRAM POSITIVE COCCI IN PAIRS code. GPCS GRAM POSITIVE COCCI IN CHAINS GPCC GRAM POSITIVE COCCI IN CLUSTERS Gram stain for Hematology night shift Page 5 of 7

6 11) Each element of the result (ie. WBCS, RBCS, GPC) should be entered as a separate observation, along with the quantity, as seen above. If you are entering codes you will have to TAB to get another line within the observation. If you are selecting from the displayed keyboard or just hitting the key you will automatically get another line within the cell. When you are done entering results in the observation, arrow down to get out of the observation. Click on the SAVE button at the bottom of the screen to file the results. 12) If you need to do a look up for a code, enter one semicolon (;) and then click on this box. 13) Enter a key word(s) in the Search value box, then just select the code you want and it will drop it into the observation. Gram stain for Hematology night shift Page 6 of 7

7 Errors in Misys GUI 1) The most frequently encountered errors are moving too quickly from one folder to the next. If you throw an error, the Messages folder will be flashing. Click on the folder and the error message will display at the bottom of the screen. Click CLOSE to remove the message from the Misys screen. Exit out of the culture and also exit out of GUI. You will also have to clear the error in LIS ALAB. 2) To clear the message in LIS ALAB, sign onto ALAB. The error will be displayed with your tech code. The errors from Misys GUI will usually have MyMRE listed as the application. 3) Hit Enter till you get to the Function prompt and enter ER. You will then get the message CONFIRM ERROR REPORT CANCELLATION. Type Y and the message will be cleared. Battery Locked in Misys GUI 1) If after you go back into GUI you find that the battery that you want to enter results on is locked, you will have to exit out of GUI again and go back onto ALAB. 2) Enter LOCK at the FUNCTION prompt. Then select Unlock an Entity. Enter the accession number that you want to unlock. You will then get the message Attempt to stop the process? (Y/N) Type Y. It will take several seconds or longer to clear. 3) Go back into GUI and enter your results. Gram stain for Hematology night shift Page 7 of 7

Isolation & Characterization of Bacteria

Isolation & Characterization of Bacteria PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!

More information

EXPERIMENT 4 STAINING TECHNIQUES

EXPERIMENT 4 STAINING TECHNIQUES Practical Manual Food Microbiology EXPERIMENT 4 STAINING TECHNIQUES Structure 4.0 Objectives 4.1 Introduction 4.2 Principle 4.3 Preparation of smear 4.4 Simple staining 4.4.1 Principle Involved 4.4.2 Materials

More information

Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692

Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Biology 4B Laboratory Bacteriological Examination of Water Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Objectives Carry out

More information

How to perform a Gram Stain. Jasleen Singh

How to perform a Gram Stain. Jasleen Singh How to perform a Gram Stain Jasleen Singh Table of Contents iii Table of Contents Table of Contents... iii Introduction... 5 Terminology... 7 Terms to be familiar with... 7 Gram Staining... 8 What is

More information

Biotechnology Science for the New Millennium by Ellyn Daugherty

Biotechnology Science for the New Millennium by Ellyn Daugherty G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biotechnology Science for the New Millennium by Ellyn Daugherty Characterizing Bacteria Using

More information

LAB NOTES FOR EXAM 1 SECTION

LAB NOTES FOR EXAM 1 SECTION LAB NOTES FOR EXAM 1 SECTION EX. 2-1: DIVERSITY AND UBIQUITY OF MICROOGANISMS Purpose: Microorganisms are found everywhere in the environment around us. To demonstrate this and to get a taste of the different

More information

DNA: Differential Extraction

DNA: Differential Extraction Sample types Prior to the organic or robotic extraction of sexual assault samples containing sperm, both the epithelial and sperm cells must be prepared. Chemicals, reagents, equipment and supplies Refer

More information

Preparing and Gram-staining a bacteriological smear

Preparing and Gram-staining a bacteriological smear College of Life Sciences and Technology Medical Laboratory Science (Applied Learning) AP 84-205-00 (52) Module 4: Practical 2015-17 Preparing and Gram-staining a bacteriological smear Date Time Class Venue

More information

Activity 5.1.4: Gram Staining

Activity 5.1.4: Gram Staining Activity 5.1.4: Gram Staining Introduction In the last activity, you isolated the bacteria that was responsible for Anna s illness and performed a gross examination of the resultant colonies. While gross

More information

Preparing and Gram-staining a bacteriological smear

Preparing and Gram-staining a bacteriological smear College of Life Sciences and Technology Medical Laboratory Science (Applied Learning) AP 84-205-00 (42) Module 4: Practical 2014-16 Preparing and Gram-staining a bacteriological smear Date Time Class Venue

More information

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE Translated English of Chinese Standard: GB4789.11-2014 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD OF THE GB PEOPLE S REPUBLIC OF CHINA GB 4789.11-2014 National Food Safety Standard

More information

Characterizing Phenotypes of Bacteria by Staining Method

Characterizing Phenotypes of Bacteria by Staining Method Experiment 3 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment 3 Characterizing Phenotypes of Bacteria by Staining Method Advisor NN Reading Chapters in BBOM 9

More information

Characterizing Phenotypes of Bacteria by Staining Method

Characterizing Phenotypes of Bacteria by Staining Method Experiment 3 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment Characterizing Phenotypes of Bacteria by Staining Method Advisor Reading NN Chapters 3.1, 3.7, 3.8,

More information

Final Approval: October 2010 Effective: October 2010 Next Review Date: August 2012

Final Approval: October 2010 Effective: October 2010 Next Review Date: August 2012 Clinical Non-gyn Procedure nongyn21.01 Preparation Of Non-Gynecologic Specimens Final Approval: October 2010 Effective: October 2010 Next Review Date: August 2012 List all stakeholder(s) and dates of approval:

More information

Geneaid DNA Isolation Kit (Yeast)

Geneaid DNA Isolation Kit (Yeast) Geneaid DNA Isolation Kit (Yeast) GEY100, GEY300 Advantages Sample: up to 2 10 8 yeast and other fungus species Yield: high yield, high quality DNA (A260/A280 = 1.8-2.0) Format: scalable DNA precipitation

More information

TD-P Revision 3.0 Creation Date: 6/9/2016 Revision Date: 8/16/2018

TD-P Revision 3.0 Creation Date: 6/9/2016 Revision Date: 8/16/2018 Protocol TD-P Revision 3.0 Creation Date: 6/9/2016 Revision Date: 8/16/2018 Electrotransformation of Agrobacterium tumefaciens Modified from Methods in Molecular Biology Vol. 47 Introduction Agrobacterium

More information

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB 4789.

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB 4789. Translated English of Chinese Standard: Translated by: www.chinesestandard.net Email: Wayne@ChineseStandard.net National Standard of the People s Republic of China GB National food safety standard Food

More information

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0

TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Cat. # 9760 For Research Use TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 4 IV. Preparation

More information

Lab 8: Bacterial Transformation with pglo for Protein Production

Lab 8: Bacterial Transformation with pglo for Protein Production OBJECTIVES: Lab 8: Bacterial Transformation with pglo for Protein Production Describe the principles of chromatography. Explain the procedure for the production of engineered proteins. Isolate the Green

More information

SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7

SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7 SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7 Question numbers refer to the applicable experiment. Questions with blanks are multiple true-false questions unless otherwise

More information

Bacterial Transformation and Protein Purification

Bacterial Transformation and Protein Purification Bacterial Transformation and Protein Purification Group 4 Natalie Beale Gregory A. Pate Justin Rousseau Dohee Won Introduction The purpose of this experiment is to perform a genetic transformation and

More information

GET Plasmid DNA 96 Well

GET Plasmid DNA 96 Well 187PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name GET Plasmid DNA 96 Well For High Yield & Quality Plasmid DNA Extraction (Cat. # 786

More information

National food safety standard Food microbiological examination: Listeria monocytogenes

National food safety standard Food microbiological examination: Listeria monocytogenes National Standard of the People s Republic of China GB 4789.30-2010 National food safety standard Food microbiological examination: Listeria monocytogenes Issued on: 2010-03 - 26 Implemented on: 2010-06

More information

PURE CULTURE TECHNIQUES

PURE CULTURE TECHNIQUES PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,

More information

ON SITE SYSTEMS Chemical Safety Assistant

ON SITE SYSTEMS Chemical Safety Assistant ON SITE SYSTEMS Chemical Safety Assistant CS ASSISTANT WEB USERS MANUAL On Site Systems 23 N. Gore Ave. Suite 200 St. Louis, MO 63119 Phone 314-963-9934 Fax 314-963-9281 Table of Contents INTRODUCTION

More information

QIAfilter Plasmid Midi Kit (Cat #: 12243)

QIAfilter Plasmid Midi Kit (Cat #: 12243) QIAfilter Plasmid Midi Kit (Cat #: 12243) Things to do before starting Add the provided RNase A solution to Buffer P1 before use. Use one vial of RNase A (centrifuge briefly before use) per bottle of Buffer

More information

Inoculate: Media. Physical State of Media: Liquid. The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory

Inoculate: Media. Physical State of Media: Liquid. The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory 1. Inoculate 2. Incubate 3. Isolate 4. Inspect 5. Identify The Five I s: Inoculate Inoculate: Media Classified according

More information

Wave and Order Picking

Wave and Order Picking Wave and Order Picking This document will cover two applications. Wave Picking offers multiple order directed picking in one run through warehouse. Order Picking is single directed order at a time. Contents

More information

EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY

EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY a) Autoclave: An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 C for around

More information

Methods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics

Methods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics Chapter 3 Topics Methods of Culturing Microorganisms Microscope (History, Types, Definitions) Staining (Gram s) Methods of Culturing Microorganisms Five basic techniques of culturing Media Microbial growth

More information

ENUMERATION OF LYMPHOCYTES SUBSETS (IMMUNOPHENOTYPING-IPT) (CD3, CD4, CD8, CD19 & CD16/56)

ENUMERATION OF LYMPHOCYTES SUBSETS (IMMUNOPHENOTYPING-IPT) (CD3, CD4, CD8, CD19 & CD16/56) Hospital Universiti Sains Malaysia for ENUMERATION OF LYMPHOCYTES SUBSETS (IMMUNOPHENOTYPING-IPT) (CD3, CD4, CD8, CD19 & CD16/56) Prepared by: Checked by: Approved by: En. Jamaruddin Mat Asan Date: Dr.

More information

Bacterial Transformation: Unlocking the Mysteries of Genetic Material

Bacterial Transformation: Unlocking the Mysteries of Genetic Material PR009 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Bacterial Transformation: Unlocking the Mysteries of Genetic Material Teacher s Guidebook

More information

CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE

CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE Jordan University of Science and Technology Faculty of Applied Medical Sciences Department of Medical Laboratory Sciences CLINICAL MICROBIOLOGY HOSPITAL LABORATORY PRACTICE Course Syllabus Course Title

More information

Wizard Genomic DNA Purification Kit

Wizard Genomic DNA Purification Kit TECHNICAL MANUAL Wizard Genomic DNA Purification Kit Instruc ons for use of Product A1120, A1123, A1125 AND A1620 TM050 Wizard Genomic DNA Purification Kit All technical literature is available on the

More information

Protocol for BelloCell-500AP Operation ver.1.0

Protocol for BelloCell-500AP Operation ver.1.0 Protocol for BelloCell-500AP Operation ver.1.0 Tabel of Content Page I. Culture Initiative and Inoculation 2-4 II. Start the Circulation System 5 III. Sampling of Culture Medium 6 IV. Cell Count by Crystal

More information

PrepSEQ Nucleic Acid Extraction Kit

PrepSEQ Nucleic Acid Extraction Kit Quick Reference Card PrepSEQ Nucleic Acid Extraction Kit Note: For safety and biohazard guidelines, refer to the Safety appendix in the PrepSEQ Nucleic Acid Extraction Kit Protocol (PN 4400739). For all

More information

HiYield TM Genomic DNA Extraction Kit Reagent

HiYield TM Genomic DNA Extraction Kit Reagent HiYield TM Genomic DNA Extraction Kit Reagent CONTENTS Genomic DNA Extraction Kit Blood Cat.No. YGBE1KR // YGBE 100R Blood Protocol..... 3 Genomic DNA Extraction Kit Cultured Cells /Tissue / Bacterial

More information

SER: Seminal Fluid ABAcard p30 Immunoassay Test

SER: Seminal Fluid ABAcard p30 Immunoassay Test Immunoassay Test Principle Equipment Reagents Controls Quality control The ABAcard p30 Immunoassay Test is a one-step test for the detection of p30. The test devices use a conjugated dye labeled antibody

More information

Standard Operating Procedure (SOP) Specimen processing of CSF, lymph nodes and other tissues for Xpert MTB/RIF

Standard Operating Procedure (SOP) Specimen processing of CSF, lymph nodes and other tissues for Xpert MTB/RIF Page: 1 of 7 Content 1. Scope 2. Definitions and abbreviations 3. Procedure 3.1 Principle 3.2 General considerations 3.3 Specimen processing 3.3.1 Lymph nodes and other tissues (Xpert MTB/RIF only) 3.3.2

More information

2 x 0.5ml. STORAGE CONDITIONS The kit is shipped at ambient temperature. Upon arrival, store the kit components as recommended on the reagent label.

2 x 0.5ml. STORAGE CONDITIONS The kit is shipped at ambient temperature. Upon arrival, store the kit components as recommended on the reagent label. 305PR 01 G-Biosciences, St Louis, MO. USA 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name OmniPrep For High Quality Genomic DNA Extraction INTRODUCTION

More information

Alkaline Lysis Large Scale Plasmid Preparation

Alkaline Lysis Large Scale Plasmid Preparation Alkaline Lysis Large Scale Plasmid Preparation 1. Set up 10 ml overnight culture. 2. Add overnight to 500 mls of sterile LB with appropriate selective agent (e.g amp, tet...) 3. Incubate at 37 C with shaking

More information

Exercise 13 DETERMINATION OF MICROBIAL NUMBERS

Exercise 13 DETERMINATION OF MICROBIAL NUMBERS Exercise 13 DETERMINATION OF MICROBIAL NUMBERS Introduction When biologists discuss the growth of microorganisms (microbial growth), they are actually referring to population size rather than to the size

More information

Lentivirus Production

Lentivirus Production Lentivirus Production Day 1 (morning): Plate 2.5X10 6 293LT cells/10cm dish. It is critical that the cells are properly maintained for optimal virus production: Cells must never grow more than 80% confluent;

More information

Warehouse and Bin to Bin Transfers

Warehouse and Bin to Bin Transfers Contents Warehouse and Bin to Bin Transfers Sales.Scanco.com Portal Settings... 2 Accessing the Application... 3 Batch number prompt - Optional... 4 Warehouse Prompts... 5 Item Entry Prompt... 6 Item Lookup...

More information

Finance Manager: Human Resources

Finance Manager: Human Resources : Human Resources Benefit Dependent Assignment This Benefit Dependent Assignment utility will do the following: link any existing dependents to a benefit group (e.g., Health, Dental, Vision, etc.) based

More information

Department of Microbiology, Lab 016 instructions

Department of Microbiology, Lab 016 instructions Protocol for standard FISH and DOPE-FISH for prokaryotes (slightly modified from Amann, 1995, note, for other modifications or other microorganisms like eukaryotes, consult special literature or check

More information

BACMAX DNA Purification Kit

BACMAX DNA Purification Kit Cat. No. BMAX044 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 212 10/2012 1 EPILIT212 Rev. A

More information

Spin Micro DNA Extraction Kit

Spin Micro DNA Extraction Kit Spin Micro DNA Extraction Kit 2-020 20 extractions 18-25 C 1. Lysis Buffer (brown cap) 2x 2 ml R 36 2. Binding Buffer (red cap) 3x 2 ml R 11/36/67 3. Protease (orange cap) lyophilized R 36/37/38 Add 250

More information

RayBio Apoptotic DNA Ladder Extraction Kit

RayBio Apoptotic DNA Ladder Extraction Kit RayBio Apoptotic DNA Ladder Extraction Kit User Manual Version 1.1 March 1, 2016 RayBio Apoptotic DNA Ladder Extraction (Cat#: 68SO-DNAL-S50) RayBiotech, Inc. We Provide You With Excellent Support And

More information

Reagent Kit Selection Guide

Reagent Kit Selection Guide Reagent Kit Selection Guide Introduction The BioChain Nucleicc Acid Preparation Technology Introduction BioChain Institute Inc. specializes in developing advanced, efficient and reliable technologies in

More information

Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces

Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa

More information

BIOSPECIMEN COLLECTION SOP Phenotype, Genotype, and Biomarkers (PGB) in ALS and Related Disorders

BIOSPECIMEN COLLECTION SOP Phenotype, Genotype, and Biomarkers (PGB) in ALS and Related Disorders The purpose of this Standard Operating Procedure (SOP) is to describe the instructions for collection, processing, and storage of collected specimens for the PGB Study. 1. Definitions 1. EDTA An EDTA tube

More information

Overview. A day in the life. 8/31/2012. Walkthrough of a typical run. Training, maintenance and QC. Ah ha! moments

Overview. A day in the life. 8/31/2012. Walkthrough of a typical run. Training, maintenance and QC. Ah ha! moments David Winkler MT(ASCP) Microbiology Technical Specialist St. Louis Children s Hospital No financial disclosures Overview Walkthrough of a typical run - Benefits to patient care, cost savings, changes in

More information

Shift Swapping Quick-Guide

Shift Swapping Quick-Guide Shift Swapping Quick-Guide (v 3.6.0) Shift Swapping Quick-Guide Login to Lawson Workforce Management Self Service: 1. Open Internet Explorer and type in http:// in the address bar to access the Lawson

More information

1 Demonstrate knowledge and understanding related to microbiological techniques. 2 Carry out techniques related to microbiology.

1 Demonstrate knowledge and understanding related to microbiological techniques. 2 Carry out techniques related to microbiology. National Unit Specification: general information NUMBER D039 11 COURSE Biotechnology (Intermediate 2) SUMMARY This unit seeks to develop knowledge, understanding and practical skills in the microbiological

More information

Quick Guide. Lesson 1 Cheek Cell DNA Template Preparation

Quick Guide. Lesson 1 Cheek Cell DNA Template Preparation Quick Guide Lesson 1 Cheek Cell DNA Template Preparation 1. Label one 1.5 ml micro test tube with your initials. Label one screwcap tube containing 200 µl of InstaGene matrix with your initials. 2. Obtain

More information

DNA Isolation Reagent for Genomic DNA

DNA Isolation Reagent for Genomic DNA DNA Isolation Reagent for Genomic DNA Phenol-free solution for rapid isolation of genomic DNA Product-No. A3418 Description DNA Isolation Reagent for Genomic DNA is a non-organic and ready-to-use reagent

More information

ProductInformation. Genomic DNA Isolation Kit. Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN

ProductInformation. Genomic DNA Isolation Kit. Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN Genomic DNA Isolation Kit Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN ProductInformation Product Description Sigma s Genomic DNA Isolation Kit isolates genomic DNA from

More information

Cellartis MSC Xeno-Free Culture Medium

Cellartis MSC Xeno-Free Culture Medium Cat. # Y50200 For Research Use Cellartis MSC Xeno-Free Culture Medium Product Manual Table of Contents I. Description... 3 II. Contents... 3 III. Storage... 3 IV. Precautions... 3 V. Materials Required

More information

ENVR 421 Laboratory #1: Basic Bacteriology Techniques

ENVR 421 Laboratory #1: Basic Bacteriology Techniques ENVR 421 Laboratory #1: Basic Bacteriology Techniques Introduction The purpose of this laboratory exercise is to familiarize you with two fundamental bacteriology techniques: the streak plate and the spread

More information

BIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014)

BIOLOGY 163 LABORATORY. THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) BIOLOGY 163 LABORATORY THE EFFECT OF ANTIBIOTICS ON THE GROWTH OF Escherichia coli B (Revised Fall 2014) Bacteria are single-celled prokaryotic organisms. As bacterial cells take in nutrients from their

More information

Silage DNA extraction procedure

Silage DNA extraction procedure Silage DNA extraction procedure Using the Fast DNA SPIN Kit for Soil by MP Biomedicals, LLC This Procedure is accompanied by a Video demonstration that can be downloaded at: http://animalscience.ucdavis.edu/faculty/robinson/videos.html

More information

topshelf2.0 QUICK REFERENCE DEVICE MANUAL

topshelf2.0 QUICK REFERENCE DEVICE MANUAL topshelf2.0 QUICK REFERENCE DEVICE MANUAL Scout topshelf2.0 Quick Reference Device Manual January 2007 2007 Scout Inc. Table of Contents 1) Introduction...6 System Requirements Initial Setup Common to

More information

NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA. National Food Safety Standard

NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA. National Food Safety Standard GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 食品安全国家标准食品微生物学检验大肠埃希氏菌计数

More information

MATERIALS AND METHODS

MATERIALS AND METHODS MATERIALS AND METHODS 2.1. Toys The toys that were used vary in material. This is particularly important when comparing and contrasting the types of bacteria that may grow on different surfaces and therefore

More information

TruSeq Custom Amplicon Low Input Workflow on the MiniSeq System

TruSeq Custom Amplicon Low Input Workflow on the MiniSeq System TruSeq Custom Amplicon Low Input Workflow on the MiniSeq System TruSeq Custom Amplicon Low Input Design Content < 1 day For Custom Panels Only Prepare Library 6.5 hours ~ 24 hours < 2 hours TruSeq Amplicon

More information

FosmidMAX DNA Purification Kit

FosmidMAX DNA Purification Kit Cat. No. FMAX046 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 204 10/2012 1 EPILIT204 Rev. A

More information

EZ-DNA. Instructions for Use. Genomic DNA Isolation Reagent. Product Description. Kit Reagent. Reagent Required But Not Supplied.

EZ-DNA. Instructions for Use. Genomic DNA Isolation Reagent. Product Description. Kit Reagent. Reagent Required But Not Supplied. EZ-DNA Genomic DNA Isolation Reagent Cat. No.: 20-600-50 Store at: Room Temperature Instructions for Use Protocol for Genomic DNA Isolation Tissue Specific Recommendations for the Use of EZ-DNA Assessing

More information

TruSeq Custom Amplicon Low Input Dual Strand Workflow on the MiniSeq System

TruSeq Custom Amplicon Low Input Dual Strand Workflow on the MiniSeq System TruSeq Custom Amplicon Low Input Dual Strand Workflow on the MiniSeq System TruSeq Custom Amplicon Low Input Dual Strand Design Content < 1 day For Custom Panels Only Prepare Library 6.5 hours ~ 24 hours

More information

THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY. STANDARD OPERATING PROCEDURE PROCEDURE NO: GLP 146 MOD: 1st Issue Page: 1 of 7

THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY. STANDARD OPERATING PROCEDURE PROCEDURE NO: GLP 146 MOD: 1st Issue Page: 1 of 7 THE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY STANDARD OPERATING PROCEDURE PROCEDURE NO: GLP 146 MOD: 1st Issue Page: 1 of 7 Procedure Type: General Laboratory Procedure 1. Risk Assessment:

More information

Prepared by Date Adopted Supersedes Procedure # Adapted from HPTN Policy. Review Date Revision Date Signature

Prepared by Date Adopted Supersedes Procedure # Adapted from HPTN Policy. Review Date Revision Date Signature APPENDIX IV: LABORATORY QUALITY CONTROL POLICY Prepared by Date Adopted Supersedes Procedure # Adapted from HPTN Policy N/A Review Date Revision Date Signature Distributed to # of Copies Distributed to

More information

Bacterial Plate Preparation. ~ Using aseptic techniques ~

Bacterial Plate Preparation. ~ Using aseptic techniques ~ Bacterial Plate Preparation ~ Using aseptic techniques ~ Bacterial Plates Laboratory and research scientists have to prepare nutrient media to grow specific strains of bacteria for their research. To do

More information

UltraTime Student Supervisor User Guide

UltraTime Student Supervisor User Guide Name: User ID: UltraTime Student Supervisor User Guide This guide will explain how to use the WebTime supervisor view of UltraTime to approve student employee hours. 8/22/2012L:\PAYROLL\Student Resources

More information

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE

More information

MACROPHAGE KILLING ASSAY

MACROPHAGE KILLING ASSAY MACROPHAGE KILLING ASSAY Updated by: Joseph Chon Date: July 2018 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND This protocol is used to determine a macrophage population

More information

Propidium Iodide. Catalog Number: Structure: Molecular Formula: C 27H 34I 2N 4. Molecular Weight: CAS #

Propidium Iodide. Catalog Number: Structure: Molecular Formula: C 27H 34I 2N 4. Molecular Weight: CAS # Catalog Number: 195458 Propidium Iodide Structure: Molecular Formula: C 27H 34I 2N 4 Molecular Weight: 668.45 CAS # 25535-16-4 Physical Description: Dark red crystals Description: Reagent used for the

More information

Preparation thin blood films and Giemsa staining

Preparation thin blood films and Giemsa staining TLM_PRO_026 Version No.001 Page 1 of 5 Department: Clinical sciences Unit: Tropical Laboratory Medicine Preparation thin blood films and Giemsa staining Project/study: Not applicable 1. Scope and application

More information

Automatic Gram staining by a

Automatic Gram staining by a Technical methods Automatic Gram staining by a linear conveyor system G. V. HEIMER AND D. A. MCSWIGGAN Public Health Laboratory, Department of Microbiology, Central Middlesex Hospital, London NWJO 7NS,

More information

Automatic Gram staining by a

Automatic Gram staining by a Technical methods Automatic Gram staining by a linear conveyor system G. V. HEIMER AND D. A. MCSWIGGAN Public Health Laboratory, Department of Microbiology, Central Middlesex Hospital, London NWJO 7NS,

More information

Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03

Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03 RECOMBINANT DNA: DUAL ANTIBIOTIC-RESISTANCE GENES Prepared by the Office of Biotechnology, Iowa State University DRAFT 4/03 ** Portions of this protocol were adapted from DNA Science: A First Course in

More information

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella

foodproof StarPrep Two Kit Order No. S Quick Reference Procedure for Legionella foodproof StarPrep Two Kit Order No. S 400 08 Quick Reference Procedure for Legionella Version 1, November 2017 A. Kit Contents / Storage and Stability Product Content Storage S 400 08 1 container with

More information

XIT Genomic DNA from Cells

XIT Genomic DNA from Cells 464PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name XIT Genomic DNA from Cells For the Isolation of Genomic DNA from Cultured Cells (Cat.

More information

Microbiological diagnosis of infectious keratitis

Microbiological diagnosis of infectious keratitis Microbiological diagnosis of infectious keratitis Pierre'e Melin Microbiology, University of Liege Medical Microbiology, University Hospital of Liege 1 Infectious keratitis Inflammation of underlying corneal

More information

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB4789.

--> Buy True-PDF --> Auto-delivered in 0~10 minutes. GB Translated English of Chinese Standard: GB4789. Translated English of Chinese Standard: GB4789.36-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD OF THE GB PEOPLE S REPUBLIC OF CHINA National Food Safety Standard - Microbiological

More information

Version 4 Reference Guide. Mobile Inspections. January 2013

Version 4 Reference Guide. Mobile Inspections. January 2013 Version 4 Reference Guide Mobile Inspections January 2013 The information in this document is subject to change without notice. The most recent version of this content may be accessed through the help

More information

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE

GB Translated English of Chinese Standard: GB NATIONAL STANDARD OF THE Translated English of Chinese Standard: GB4789.36-2016 www.chinesestandard.net Sales@ChineseStandard.net NATIONAL STANDARD OF THE GB PEOPLE S REPUBLIC OF CHINA GB 4789.36-2016 National Food Safety Standard

More information

Physical Count by Bin User Manual Table of Contents

Physical Count by Bin User Manual Table of Contents Physical Count by Bin User Manual Table of Contents Scanco Portal setting... 2 Main Applications Physical Count Icon... 3 Count by Bin... 4 Warehouse Prompt... 5 Warehouse Lookup... 6 Starting Bin Prompt...

More information

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector

ITS Sequencing in Millepora. 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector Page 1 of 5 10/09 Subcloning DNA Fragments into pbluescript Preparation of pbluescript Vector 1. Digest 1 µg of pbluescript with Eco RI 2. Following digestion, add 0.1 volumes of 3M sodium acetate (ph

More information

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No for isolation of total RNA from 50 samples Cat. No. 1 88 665 Principle A single reagent lyses the sample lysis and inactivates RNase. In the presence of a chaotropic salt (guanidine HCl), the released

More information

Instructions For Use

Instructions For Use Beer Spoilage Micro Test Product Code: PM-02 Instructions For Use Introduction Beer spoilage caused by wild yeasts and bacteria is a major concern to both commercial and home brewers. Microbial spoilage

More information

Applicable To Employees of the Gundersen Tri-County Hospital clinical laboratory and Gundersen Palmer Lutheran Hospitals and Clinics laboratories.

Applicable To Employees of the Gundersen Tri-County Hospital clinical laboratory and Gundersen Palmer Lutheran Hospitals and Clinics laboratories. Subject BACTEC 9050 Index Number Lab-8681 Section Laboratory Subsection Regional Clinic/Affiliate Hospital Laboratories Category Departmental Contact Blum, Rachel Last Revised 2/13/2017 References Required

More information

Product # Specifications. Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 700 µl

Product # Specifications. Kit Specifications Column Binding Capacity 50 µg Maximum Column Loading Volume 700 µl 344 Merritt Street St. Catharines, ON, Canada L2T 1K6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Leukocyte RNA Purification Kit Product # 21200 Product Insert

More information

Create New Pallet \ Add Items to existing Pallet

Create New Pallet \ Add Items to existing Pallet Create New Pallet \ Add Items to existing Pallet Contents Sales.Scanco.com Portal Settings... 2 Accessing the Application... 3 Multibin Pallet Type Entry... 4 Pallet Type Lookup... 4 Pallet ID prompt...

More information

Microorganisms In Our Environment

Microorganisms In Our Environment PR015 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Microorganisms In Our Environment Teacher s Guidebook (Cat. # BE 106) think proteins!

More information

INVITATION TO BID. (This is not an order.)

INVITATION TO BID. (This is not an order.) Page 1 of 10 SCHEDULE OF EVENTS Event Description 1 / 1 Roche Diagnostics POC Chemstrip Urine Test Strips 3.00000 / Event Date For Use With (Application): Urinalysis CLIA Complexity: Waived Product Type:

More information

Blood and Tissue Parasites Issued by: LABORATORY MANAGER Original Date: March 13, 2000 Approved by: Laboratory Director Review Date:

Blood and Tissue Parasites Issued by: LABORATORY MANAGER Original Date: March 13, 2000 Approved by: Laboratory Director Review Date: Section: Policy # MI\PAR\11\01\v01 Page 1 of 5 Subject Title: Laboratory Procedures for Blood and Tissue Parasites Issued by: LABORATORY MANAGER Original Date: March 13, 2000 Approved by: Laboratory Director

More information

GB Translated English of Chinese Standard: GB

GB Translated English of Chinese Standard: GB Translated English of Chinese Standard: GB4789.35-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.35-2016 National food safety standard

More information

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only.

I-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only. Instruction Manual Ver. 05.11.17 For Research Use Only I-Blue Midi Plasmid Kit & I-Blue Midi Plasmid Kit (Endotoxin Free) IB47180, IB47190 (2 Preparation Sample Kit) IB47181, IB47191 (25 Preparation Kit)

More information

Bacterial Transformation Protocol 2

Bacterial Transformation Protocol 2 26 BACTERIAL TRANSFORMATION USING FLUORESCENT PROTEIN Bacterial Transformation Protocol 2 Group # Role in Group Materials Reader Timer Technician Student Name Materials checklist (1) ScienceBridge Transformation

More information

Presto 96 Well gdna Bacteria Kit

Presto 96 Well gdna Bacteria Kit Presto 96 Well gdna Bacteria Kit 96GBB02 (2 x 96 well plates/kit) 96GBB04 (4 x 96 well plates/kit) 96GBB10 (10 x 96 well plates/kit) Instruction Manual Ver. 05.04.17 For Research Use Only Advantages Sample:

More information