GENECHECKER Ultra-Fast PCR System

Transcription

1 "Gives You a PCR Result in 11 Minutes." GENECHECKER Ultra-Fast PCR System PCR Innovation Starts Here! Do it Anywhere. Make it Faster. See the Result. Patented Chip Based Design Provides Extremely Rapid Output "30 Cycles in 12 Minutes" No Need for Gel Electrophoresis - Instant Verification of PCR Result with Your Eyes Innovative and Compact Design for Laboratory and POCT Applications Intuitive Control with LED Indicated Jog-Dial and LCD Display Conventional and Reverse Transcriptase(RT) PCR with Single Device

2 WHY GENECHECKER? Dongwoo Science is proud to introduce GENECHECKER Ultra-Fast PCR System. This instrument is an innovative solution for molecular diagnostics research applications.this adopts special polymer chip named Rapi:chip which enables even faster thermal treatment of samples in it than the case of using conventional PCR tubes. In addition, this instrument took the advantage of real-time PCR by integrating high-end fluorescence detection technology in it, which enabled users to visually identify PCR result through the window on the top of instrument. Using key technologies integrated in it, GENECHECKER successfully performs 30 thermal cycles in 12 minutes and lets you to confirm the qualitative PCR result with your eyes.model UF-100 GENECHECKER Ultra-Fast PCR System has compact and portable design and can be carried to any location conveniently. This instrument adopts DC 12V power input and can be connected to general car power source when optional car power connection kit is equipped. Accordingly, user can drive to the site where sample exists and perform on-site PCR tasks in his or her car right after sample collection and necessary preparation process. GENECHECKER Ultra-Fast PCR System Motives of Development Development of this state-of-art instrument was started from several motives. Researchers were spending too much time and efforts to get a PCR result. Typically, researchers had to spend over 40 minutes for thermal cycling and spend another 40 minutes for gel electrophoresis, which was quite time consuming job to get a simple PCR result. Other motive was to prove that chip based PCR technology could sufficiently cover the function of real-time PCR instruments in terms of qualitative analysis. We could get convinced that targeted performance could be achieved when this unique chip based PCR concept meets high-end fluorescence detection technology and it was proven by field evaluations in variety of molecular diagnostics applications. Design Innovation Design of GENECHECKER is unique and modern. Design efforts to eliminate unnecessary features made this instrument look quite simple. Control is intuitively made by jog-dial which enables users to easily perform most of parameter setting. Just handle the jog-dial to set up your own protocol and enjoy innovative design of this instrument in your traditional laboratory environment.

3 GENECHECKER vs. Conventional PCR Thermal Cycler Time of Thermal Cycling (30 Cycles) Result Confirmation Total Time Needed to Get a Result Qualitative Analysis Qualitative Analysis Sample Carrier Number of Sample per Reaction Volumetric Capacity of Well Required Volume of Sample Mixture Analysis on the Site (POCT) This is Right Solution for You. GENECHECKER Less than 12 Minutes Instantly Possible through Well-Viewer Less than 12 Minutes Instantly Possible Possible after Electrophoresis Proprietary Chip (Rapi:chip ) 8 (Suitable for Instant Analysis) Possible through Car Power Jack Conventional PCR Approx. 40 Minutes Possible after Gel Electrophoresis More than 90 Minutes Possible after Gel Electrophoresis Possible after Electrophoresis PCR Tube (Depending on Tube Size) No Less than 20 Not Recommendable GENECHECKER was born from the understanding of researchers challenges. Optimized chip based thermal cycling maximizes heat exchange between sample and heating plate, which dramatically reduces the time required for sufficient amplification of genes. Integrated fluorescence detection interface lets users visually confirm the expressed gene right after thermal cycling. See the difference and experience the new formula for molecular diagnostics researches. Applications of GENECHECKER General Research on Molecular Diagnostics Technologies Laboratory PCR in Life Science and Molecular Biological Study Field Applications for Veterinarian Diagnostics Agriculture(GMO) and Food Analysis Preparative Analysis before High Throughput PCR Task Pharmaceutical or Biological Quality Control On-site Pathogen Detection (POCT) Ordering Information Part Number Description Pack Size Model UF-100 GENECHECKER Ultra-Fast PCR System 1 SET Rapi:chip PCR Chip for GENECHECKER Small Pack Rapi:chip PCR Chip for GENECHECKER Medium Pack Rapi:chip PCR Chip for GENECHECKER Large Pack Car Power Kit for GENECHECKER POCT Kit for GENECHECKER 48 PCS 384 PCS 768 PCS 1 EA 1 SET

4 1_ LCD Display 4line text LCD offers clear identification while protocol setting and status monitoring 2_Jog-Dial Rotating jog-dial in clockwise direction moves cursor on the display down (right) or increases set valus. Rotating jog-dial in counter-clockwise direction moves cursor on the display up (left) and decreases set valus. Pressing jog -dial selects the menu where cursor is located. 3_LED Indicator LED indicator is illuminated in two different colors - blue and red. Blue LED indicates that the instrument is idle and ready for use. Red LED indicates that the instrument is in use, i.e.pcr cycle is being performed. LED is turned off when prompted PCR cycles are finished and redult is available for observation through Well-Viewer. 4_MENU button Main menu screen is displayed when this button is pressed from initial screen. Displayed screen moves to previous step when this button is pressed. 5_RUN/STOP button Selected PCR protocol is executed when this button is pressed. Currently performed PCR cycle is immediately stopped when this button is pressed while the instrument is running. 6_Well-Viewer Window After PCR cycles are completely performed,users can identify the change of well colors with their eyes through this window. Patent pending fluorescence detection technology is integrated inside this part of instrument. Wells Expressed Wells Not Expressed 7_PUSH bar Chip drawer is gently opened and upper sealing cap on the heating plate is automatically moves up when this bar is softly pressed. 8_Chip Presser Automatically presses upper surface of chip when the drawer is closed. General Specification of GENECHECKER Operating Mechanism Control Temperature Accuracy ± 0.5 Temperature Uniformity ± 0.5 (Well to Well) Temperature Stability ± 0.5 9_Heating Plate This is the point where the chip is loaded for PCR cycles. Flat surface is heated and cooled during thermal cycling. 10_Groove for Easy Handling This part is to enhance portable characteristic of instrument. 11_Air Ventilation Holes Holes which air flows into the instrument through. 12_Drawer This is the module transporting chip into the instrument. Mechanically stable and robust design offers soft movement and maintenance-free features of this frequently used part. Ramp up Speed Ramp down Speed Range of Temperature Setting Type of Sample Carrier Max. Samples per Reaction Required Sample Volume Verified PCR Time Necessity of Gel Electrophoresis Display Integrated Memory Power Power Consumption Dimension / Weight 8.0 / second 8.0 / second 35 ~ 65 (1.0 Increment) for RT Step 20 ~ 99 (1.0 Increment) for PCR Polymer Based 3-Dimensional Microfluidic Chip 8 Less than 12 Minutes for 30 Cycles (without RT Step) No (Instant Qualitative Analysis) Text LCD (4 Line) Saves up to 12 Customized PCR Protocols AC 110/230V and 50-60Hz, 1.5A Input DC 12V, 10A Output 70W 200(w) x 200(d) x 125(h) mm / 3.0kg

5 What is Rapi:chip? Rapi:chip is an essential component to get an ultra-fast PCR result using GENECHECKER. This is designed from long-time experience of designing lab-on-a-chip devices and enables GENECHECKER to perform its extremely fast thermal cycling in efficient way. This unique chip has 8 wells and each well accommodates of sample mixture which is still less amount than the case of using typical PCR tubes. Bottom surface of this chip has only 150μm of thickness, which facilitates efficient heat exchange between sample and heat block. Printed Well Numbers Well number is printed on the upper surface of chip for easy identification. Wings These parts are used for easy handlingwith fingers or tweezers. Apertures Pipette tip fits in these apertures so that sample and reagent could be loaded or collected. Key features of Rapi:chip Optimized to Reduce PCR Time (Thin and Wide Surface for Thermal Treatment) Require Minimized Volume of Sample and Reagent (Well Volume : ) Compact and Flat Design Makes Easy-to-Handle Contamination Free and Transformation Free (Freezable) High Transparency Polymer Material for Easy Observation of Well Unique 3-Dimensional Chip Structure Maximizes Luminous Efficiency General Specification of Rapi:chip Size (W x D x H) 38mm x 25mm x 6mm Weight 3.56 g Number of Wells 8 Wells Volume of Each Well Aperture Diameter Channel Height 0.5 mm Channel Length 8 mm Channel Width 2 mm Material Transparent Acryl (Transparency : 95% +)

6 CONTACT Dongwoo Science Co., Ltd. 747 ITECO, 762 Deokpung-dong, Hanam-si, Gyeonggi-do, , Korea phone fax web Elchrom Scientific AG Gewerbestrasse 8, CH- 6330, Cham phone fax web