CERTIFICATE OF ANALYSIS

Transcription

1 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67600M Lot #: 32L34005 Description: Phosphothreonine (Set of 3 MAbs) Set Includes: Catalog #K67101M Catalog #K67141M Catalog #K67341M Clone BDI101 Clone BDI141 Clone BDI341 Specificity: Clone: Phosphothreonine Detection kit combines 25ug of each of the monoclonal anti-phosphothreonine antibodies. Phosphorylation Patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. SETTHR Host Animal: Mouse Isotype: n/a Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Applications: Tissue culture Phosphothreonine conjugated to KLH Purified, Lyophilized. Reconstitute with 1ml water per vial for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography Not applicable Not determined Lyophilized from 2X PBS, PEG and Sucrose 0.09% Sodium Azide (prior to lyophilization) Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 4000, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

2 Catalog #K67600M Page 2 of 2 Storage: Warning: References: Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored at 2-8 C for up to 3 months. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. The reference listed below is for research purposes only. Peters, G.A., et al., (2006), Phosphorylation of Specific Serine Residues in the PKR Activation Domain of PACT is Essential for its Ability to Mediate Apoptosis, The Journal of Biological Chemistry, 281(46): Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

3 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67101M Lot #: 1E13605 Description: Specificity: Clone: MAb to Phosphothreonine Monoclonal Antibody to Phosphothreonine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on threonine residues is mediated by serine/threonine kinases. Clone BDI101 recognizes a broad range of threonine-phosphorylated proteins in crude cell extracts, providing a valuable tool for non-radioactive phosphothreonine detection. BDI101 Host Animal: Mouse Isotype: IgG 1, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Applications: Tissue culture Phosphopeptide conjugated to KLH Purified, Lyophilized. Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography. Not applicable. Not determined. Lyophilized from 2X PBS, PEG and Sucrose. 0.09% Sodium Azide (prior to lyophilization) Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 4000, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

4 Catalog #K67101M Page 2 of 2 Storage: Warning: Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored at 2-8 C for up to 3 months. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

5 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67141M Lot #: 1E13605 Description: Specificity: Clone: MAb to Phosphothreonine Monoclonal Antibody to Phosphothreonine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on threonine residues is mediated by serine/threonine kinases. Clone BDI141 recognizes a broad range of threonine-phosphorylated proteins in crude cell extracts, providing a valuable tool for non-radioactive phosphothreonine detection. BDI141 Host Animal: Mouse Isotype: IgM, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Applications: Tissue culture Phosphopeptide conjugated to KLH Purified, Lyophilized. Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography. Not applicable. Not determined. Lyophilized from 2X PBS, PEG and Sucrose. 0.09% Sodium Azide (prior to lyophilization) Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 4000, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

6 Catalog #K67141M Page 2 of 2 Storage: Warning: Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored at 2-8 C for up to 3 months. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY

7 5171 Wilfong Road Memphis, TN Telephone: Fax: CERTIFICATE OF ANALYSIS Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Catalog #: K67341M Lot #: 1E13605 Description: Specificity: Clone: MAb to Phosphothreonine Monoclonal Antibody to Phosphothreonine Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on threonine residues is mediated by serine/threonine kinases. Clone BDI341 recognizes a broad range of threonine-phosphorylated proteins in crude cell extracts, providing a valuable tool for non-radioactive phosphothreonine detection. BDI341 Host Animal: Mouse Isotype: IgG 1, kappa Source: Immunogen: Format: Purification: Concentration: Affinity Constant: Buffer: Preservative: Applications: Tissue culture Phosphopeptide conjugated to KLH Purified, Lyophilized. Reconstitute with 1ml water for 15 minutes at room temperature. Thiophilic adsorption and size exclusion chromatography. Not applicable. Not determined. Lyophilized from 2X PBS, PEG and Sucrose. 0.09% Sodium Azide (prior to lyophilization) Western blot: 1ug/ml for HRP/ECL detection. Do not use milk/casein for blocking and dilution. Recommended blocking buffer: 3% BSA, 1% PVP-10 (polyvinylpyrrolidone), 1% PEG 4000, 0.1% Tween 20 in 2X PBS. Immunoprecipitation: use at 1-10ug per 10 6 vanadate treated A431 cells. ELISA: use at 0.05ug/ml Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

8 Catalog #K67341M Page 2 of 2 Storage: Warning: Store lyophilized product at 20 C. After reconstitution, aliquot and store at 80 C. Thawed aliquots may be stored at 2-8 C for up to 3 months. Avoid multiple freeze/thaw cycles. This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. Signature 30 September 2013 Date FOR RESEARCH OR FURTHER MANUFACTURING USE ONLY