A simple device for injecting small volume (5-100 micro l) of liquid in small laboratory animals
|
|
- Virgil Curtis
- 6 years ago
- Views:
Transcription
1 A simple device for injecting small volume (5-100 micro l) of liquid in small laboratory animals Jashdeep Bhattacharjee, Barun Das, P Nagarajan, Pramod Upadhyay National Institute of Immunology Aruna Asaf Ali Marg New Delhi , India pkumar@nii.ac.in Key words : Injecting small volume, Small laboratory animal, tail vein injection, retro-orbital venous sinus injection Abstract Intense practice is required to achieve good success rate in injecting small volume (5-100μl) of liquid in small laboratory animals. We have designed a device for making such injections incident free with minimal skills. In this device, the piston of the syringe is pushed by a pushing mechanism based on the cable release of SLR camera. To use this device, the experimenter holds the animal with one of the hands and the other hand holds the syringe with needle mounted on the device. After piercing the target site at blood vessel or the organ the experimenter becomes still and piston of the syringe is either pushed by another person from a distance or by a syringe pump energized through a foot switch. We have evaluated this device for delivering injections in the tail vein and retro-orbital venous sinus in mice. The accuracy of the delivered dose was high and the injection procedure was completed in lesser time when the injector device was used.
2 A device for injecting small volume (5-100 micro liter) of liquid in small laboratory animals Introduction Injecting small volumes of samples (drugs, plasmids, cell suspension etc.) at a precise location (tail vein, retro-orbital venous sinus, spleen, etc.) of small animal (1) such as mice is often challenging. Delivering such injections without any incidents requires intense practice and the success rate is not consistent. There are two critical steps which determine the success of these injections, piercing the needle at a very precise location followed by pushing the piston of the syringe to deliver the liquid (a drug or cell suspension) at the pierced location. Often, even after piercing the tissue at the accurate site incidents of wrong administration might occur due to dislocation of the needle during pushing the piston of the syringe. Normally we use one hand to adjust the animal in order to orient the site of injection towards the needle and the needle is pierced by the other hand. The difficulty comes when the experimenter needs to push the piston; to do this, hand holding the syringe has to be moved (Figure 1A). This movement causes most incident or failure of the injection. Figure 1A. Difficulty experienced when an injection is delivered; one of the hands has to move to push the piston of the syringe. There are a variety of syringe pumps which can hold 1ml syringe in the market and a few of those pumps are portable. But these syringe pumps are mostly infusion pumps and are designed to deliver the dose over a period of time. Most of the portable syringe pumps are sufficiently bulky and cannot be used directly to deliver injections to small animals. Hammond and Bhattacherjee (2) described a very nice design of a micro-capillary injection apparatus which can deliver microliter volume to the anterior chamber or vitreous body of the eye of small animals. Recently, World Precision Instruments, Florida, USA has introduced a microsyringe pump, UMP-III which can deliver small volumes. In these devices, fine size needles (33-36 gauge) are used and these are only suitable for delivering drugs etc. and are not appropriate for delivering cell suspensions.
3 To address this need, we have designed a device to deliver small volume (5-100 l) of liquid in small laboratory animal. In this device conventional 1 ml insulin syringe with 30 gauge needle is used and it can deliver small volumes of liquids ( drug solutions, cell suspensions etc). In order to use this device, the experimenter needs to hold and orient the site of injection of the anaesthetized animal towards the needle with one hand and uses other hand to hold the syringe mounted in the device to pierce the tissue and remains still, the piston of the syringe is pushed through a pushing mechanism (Figure 1B) made of a cable release used in SLR camera. The holding weight of this device with cable release is around 8 grams only which makes it as easy as holding a syringe. Figure 1B. The piston pushing mechanism in which no movement is required to push the piston after piercing the retro-orbital venous sinus. The cable release can also be pushed by mounting it on a typical syringe pump (Figure 1C) which has been adjusted to move the piston at a desired speed and is electrically energized by pressing a foot switch. Figure 1C The cable release mounted on a typical syringe pump.
4 Due to the simple design of this device, it can easily be fabricated for less than 10 USD and modified to meet unique experimental situations. Materials and Methods Construction of the device Items required 1. Threaded cable release used in SLR camera of the type Nikon AR-3 2. Machined pieces of acrylic sheet of the dimensions given in Figure 2A. Figure 2A. Dimensions of the different pieces of device. 3. Cyanoacrylate adhesive to join the pieces of exactly cut acrylic sheet. 4. Plastic tube locker used in blood bags ml Insulin Syringe, 26/30G (Dispo van, India) 6. Syringe Infusion Pump of type PHD 22/2000 (Harvard Apparatus, MA; US) operated by the foot switch of the type RS Stock No (Optional). Get the exact sizes of acrylic sheet cut from a machining shop and carefully join them using the Cyanoacrylate adhesive. Fix the cable release in its hole and the device is ready to use. Assembled device is shown in Figure 2B.
5 Figure 2B. The assembled device. Using the device 1. Release the cable by pressing the hold rim. 2. Fill the desired dose (not more than 100 l) in a 1 ml syringe and cap the needle. 3. Place the syringe in its slot on the device and apply the tube locker to firmly place the syringe in its slot. 4. Hold the device with filled syringe in one hand, uncap the needle and hold the animal by other hand. Pierce the desired tissue and become still. Instruct the other person to push the release cable to deliver the dose. Please see Video V1. 5. Alternatively, properly fix the free end of release cable in a typical syringe infusion pump which is wired through a foot switch. On the syringe pump, select the syringe size and flow rate so that the cable release moves at a desired speed. After piercing the tissue and becoming still, press the foot switch to energize the syringe pump so that it pushes the release cable to deliver the dose. Results and Discussion We have evaluated this device for performing injection in the tail vein and retro-orbital venous sinus in mice. These injection procedures have been approved by the Institutional Animal Ethics Committee of the National Institute of immunology, New Delhi, India. We have compared (i) the variability in the delivered dose and (ii) the time required to complete the injection procedure as measured by the time for which animal was held and the time for which the animal was pricked. These timings are very important for maintaining overall wellbeing of the animals during experiments. Figure 3 and 4 show the summary of results when tail vein and retro-orbital venous sinus injections were performed in NOD mice (N=10) with and without using this device. Figure 3 shows the comparison of dose delivered. For retro-orbital venous sinus injections, the variation in injected volume per injection, as indicated by standard deviation, was 10 times less when the injector device was employed. The scenario was comparable for tail vein injections.
6 Figure 3. A comparison of dose delivered when 100 l dose was delivered in retro-orbital venous sinus and tail vein in mice (N=10) with and without injector device. A comparison of the time required for completing the injection procedure is shown in Figure 4. For tail vein injections, in both groups, on average the animals were held for around 25 seconds but the variations in the time durations was 4 times less when the injector device was employed. Similarly, the animal was pricked for around 16 seconds and the standard deviation was half when the injector device was employed. Also, noticeable reductions in the standard deviations of these timings were observed when the injector device was used for delivering retro-orbital venous sinus injections. Figure 4. A comparison of the time required for completing the injection procedure with and without injector device.
7 Overall, when injector device was employed retro-orbital venous sinus injections the accuracy of the delivered dose was high and the injection procedure was completed in less time. The utility of this device is further enhanced when the retro-orbital venous sinus injections are reported to be less stressful than the tail vein injections (3). Further, due to high accuracy of delivered dose in less time, this injector device is very useful for delivering cells or bio-reagents during surgical procedures. The video V1 shows how this device was used for intrasplenic hepatocyte transplantation in mice. Video V1. A still image from the video showing intrasplenic hepatocyte transplantation in mice carried out with injector device. References 1. Turner, P.V., Brabb, T., Pekow, C., et al. Administration of substances to laboratory animals: routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science, 2011, 50, Hammond, B.R. and Bhattacherjee, P., A method for the intraocular injection of micro-volumes in small animal species. Current Eye Research, 1982, 1, Steel, C.D., Stephens, A.L., Hahto, S.M., et al. Comparison of the lateral tail vein and the retroorbital venous sinus as routes of intravenous drug delivery in a transgenic mouse model. Lab Anim (NY), 2008, 37(1),
Policy 19 Fluid Administration and Collection in Rodents Version 1.0 Approval Date: 12/12/12
Policy 19 Fluid Administration and Collection in Rodents Version 1.0 Approval Date: 12/12/12 Purpose This policy was designed to provide investigators with reference values related to the administration
More informationSOP: Mouse Intravenous Injections
SOP: Mouse Intravenous Injections These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to investigators during
More informationSOP: Intravenous Injections in the Rat
SOP: Intravenous Injections in the Rat These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to investigators during
More informationGuidelines for Survival Bleeding of Mice and Rats
Guidelines for Survival Bleeding of Mice and Rats These guidelines have been developed to assist investigators and Institutional Animal Care and Use Committees (IACUC) in their choice and application of
More informationSOP: Blood Collection from the Tail Vein in Rats
SOP: Blood Collection from the Tail Vein in Rats These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to investigators
More informationComplete Solutions for Small Animal Infusion
Complete Solutions for Small Animal Infusion NEW! Full Line of Catheters Swivels and Infusion Cages Vascular Access Products and Tethers Tubing and Connectors Jackets Syringe Pumps Surgical Instruments
More informationDelivery portal. Flow moderator. Impermeable reservoir wall. Osmotic agent. Semipermeable membrane. Reservoir. 3 days. 1 day. 4 weeks. 1 week.
1 Delivery portal Flow moderator Impermeable reservoir wall Osmotic agent Semipermeable membrane Reservoir 1 day 3 days 1 week 2 weeks 4 weeks 6 weeks 2 Dimensions, Components, and Materials of ALZET Osmotic
More informationCole-Parmer Pro-Spense Disposable Syringe Pump User s Manual. Model # (100ml), # (260ml) Publication REV-A
Cole-Parmer Pro-Spense Disposable Syringe Pump User s Manual Model #74905-46 (100ml), #74905-47 (260ml) Publication 5607-008-REV-A Contents Caution...2 Product Purpose and Assembly...2 Specifications...2
More informationFamily Secrets. Part 4 Testing for the HD Gene
Family Secrets Part 4 Testing for the HD Gene Narrator: Jenny, Jeremy, and their father have decided to undergo genetic testing for Huntington s disease. Today, our lab groups will act as gene testing
More informationRodent restrainers. Cylindrical restrainers: Broome Restrainers. Pastry bag restrainer: DecapiCone
Clinical Techniques Rodent restrainers Cylindrical restrainers: Broome Restrainers Pastry bag restrainer: DecapiCone Rabbit restrainers Needles Gauge refers to diameter Smaller diameter is the larger number
More informationCalcium-EDTA Tutorial for the MicroCal ITC200
Calcium-EDTA Tutorial for the MicroCal ITC200 Getting Started 1. The computer and the ITC200 are normally left on. If they have been switched off for any reason makes sure that you boot them in the order
More informationWater-Water Titration for the MicroCal ITC200
Water-Water Titration for the MicroCal ITC200 Goal: Water-Water titrations are a good way to check for possible problems, especially the cleanliness of the ITC and your skills in cell filling. If a water-water
More informationSyringe Pumps. Please contact us for help in selecting the best pump for your application and a free quote.
Syringe Pumps Microfluidic Pumps! We are proud to offer select Harvard Apparatus syringe pumps for use with SynVivo biochips. These high quality pumps have been extensively tested and offer a feature rich
More informationPRODUCT DATA SHEET. SikaFuko Eco-1 PRODUCT- DESCRIPTION. Re-injectable hose for sealing construction joints in watertight structures
PRODUCT DATA SHEET SikaFuko Eco-1 Re-injectable hose for sealing construction joints in watertight structures PRODUCT- DESCRIPTION USES PRODUCT CHARACTERISTICS TESTING & APPROVALS Re-injectable hose system
More informationINSTITUTIONAL ANIMAL USER TRAINING PROGRAM Online Courses and Hands-On Workshops Outline
INSTITUTIONAL ANIMAL USER TRAINING PROGRAM Online Courses and Hands-On Workshops Outline - 2018 Table of Contents I. Institutional Animal User Training Program Steps... 3 II. Governing Institutional Policies
More informationN Scale Double Track Lift Bridge v1.2
N Scale Double Track Lift Bridge v1.2 3.25"W x 16"L x 7.5"H Plus two 5" long approach bridges Instructions for assembly of the Lift Bridge Included in this kit: Instructions and part diagrams 138ea. 1/16"
More informationBlow-Fill-Seal Machine
Blow-Fill-Seal Machine 3 processes combined in one fully automatic machine, which is simply referred to as Blow-Fill-Seal. Many refer to it as Form-Fill-Seal. Life-Saving Medications: Aseptic Blow-Fill-Seal
More informationContract Manufacturing Services for Pre-filled Syringes
Contract Manufacturing Services for Pre-filled Syringes From Standard Solutions to Complex Products Stefan Czvitkovich, PhD Director Product Partnering Sterile Pharmaceuticals Central Europe CPhI woldwide
More informationSteam Quality Test Kit
Steam Quality Test Kit Page 1 The Steam Quality Test Kit is equipped with all the component parts that are necessary to perform the required tests and report the results in accordance with ISO EN 285,
More informationEQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY
EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY a) Autoclave: An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 C for around
More informationCIA Induction in DBA/1 Mice
Recommended protocol for use with: or Hooke Kit Chicken Collagen/CFA Emulsion (EK-0210) and Hooke Kit Chicken Collagen/IFA Emulsion (EK-0211) Hooke Kit Bovine Collagen/CFA Emulsion (EK-0220) and Hooke
More informationDesigning Auto-Injectors for Multiple Drug Viscosities
Medical device OEM s and contract manufacturers: Designing Auto-Injectors for Multiple Drug Viscosities David Philbrick Business Development Manager Components specifically springs used for device activation
More informationSafer, easier and faster drug delivery Weibel CDS AG develops and produces innovative, user friendly, application oriented Injections Systems and
Safer, easier and faster drug delivery Weibel CDS AG develops and produces innovative, user friendly, application oriented Injections Systems and Devices. upgrades your vial practically to a syringe Vials
More informationInsulin Syringes ACCU-SHOT TM ACCU-SHOT U40 & U100. (with Ultra Thin & Short Needle) Sizes. Colour Code of Top Cap ml U G 6 mm Red
Be aware be safe... SPM Medicare Pvt. Ltd. (An EN ISO 13485:2012 Certified Company) Corp. & Mfd. Address: B-40, Phase-II, NOIDA, U.P., INDIA Customer Care: +91-120-4202413 Insulin Syringes ACCU-SHOT U40
More informationGen Bio 1 Lab #9: CSI and Strawberry DNA Analysis
Name: Date: Gen Bio 1 Lab #9: CSI and Strawberry DNA Analysis Pre-Lab Reading: Page 412-416 in the 10 th edition of Campbell Biology Pre-Lab Vocabulary: 1. Agarose DNA gel 2. Gel Electrophoresis 3. Endonuclease
More informationHydro-wind Education Kit ASSEMBLY GUIDE
Hydro-wind Education Kit ASSEMBLY GUIDE Battery operation instructions: Model No.: FCJJ-56 Warning To avoid the risk of property damage, serious injury or death: This kit should only be used by persons
More informationImaging Guide. 2D Target-Ready Contrast Agent imaging using the Vevo 3100 System
Imaging Guide 2D Target-Ready Contrast Agent imaging using the Vevo 3100 System System Compatibility: This guide contains instructions and suggestions for work on the Vevo 3100 and transducers from the
More informationMicroFluidics. Product Overview MASS FLOW CONTROLLERS SOLENOID CONTROL VALVES SOLENOID VALVES PROCESS VALVES PNEUMATICS SENSORS
Product Overview MicroFluidics SOLENOID VALVES 01 PROCESS VALVES 894450 Version 04/2015 Print 04/2015 www.scanner-gmbh.de Bürkert Fluid Control Systems Christian-Bürkert-Straße 13-17 74653 Ingelfingen
More informationRolling, Adhesion and Migration Assay Using SynRAM Idealized Network Kits and Chips Technical Manual Catalog #s , , SR
Rolling, Adhesion and Migration Assay Using SynRAM Idealized Network Kits and Chips Technical Manual Catalog #s 401002, 401001, 102008-SR Schematic of the SynRAM model Chip. Vascular channels are for culture
More information3D Blood Brain Impedance Assay Using SynBBB Idealized Network (TEER configuration) Kits and Chips Technical Manual Catalog #s 402004, 402003, 102015-SB Schematic of the chip used for the SynBBB Model and
More information06 Vascular Access Safety Products 07 Vascular Access Conventional. p24 OPTIVA I.V. Catheters. p24 JELCO Striped I.V. Catheters
Sharps Safety Safety Devices for Hypodermic Injections Safety Devices for Insulin and TB Injections Safety Devices for Prefilled Medication Injections Safety Devices for Venous Blood Collection Safety
More informationTransplantation of Axol TM hnpcs into neonatal rat cerebral cortex
Experts Protocol Series Transplantation of Axol TM hnpcs into neonatal rat cerebral cortex Application Protocol Kindly provided by Melissa R Andrews, University of St Andrews Appli catio n Prot Table of
More informationRolling, Adhesion and Migration Assay Using SynRAM Microvascular Network Kits and Chips Technical Manual Catalog #s , , SR
Rolling, Adhesion and Migration Assay Using SynRAM Microvascular Network Kits and Chips Technical Manual Catalog #s 401004, 401003, 105001-SR Tissue Compartment Vascular Channel Overview of Assay Immune
More informationSubstation #9 Instructions for Assembly of the HO scale kit. v1.1
Substation #9 Instructions for Assembly of the HO scale kit. v1.1 Kit Contents: 26 each.090" Acrylic parts 2 each.060" Acrylic parts 1 each.060" Acrylic parts sheet 1 each.020" Adhesive backed styrene
More informationBehaviour, housing and care of rodents and rabbits
Kurs i Forsøksdyrlære, Universitetet i Bergen, Lars Friis Mikkelsen 19 March 2010 Slide no 1 Behaviour, housing and care of rodents and rabbits Lars Friis Mikkelsen, DVM, MLAS Laboratory Animal Veterinarian
More informationGuideline on the label design for Injections (Draft for Comments)
Guideline on the label design for Injections (Draft for Comments) 2018.3 Content Foreword... 3 1. Scope... 4 2. Terms and definitions... 4 3. Basic requirements for labels... 4 4. Design principles of
More informationBD PhaSeal System. Product Catalog
BD PhaSeal System Product Catalog BD PhaSeal is a clinically proven closed-system drug transfer device that prevents hazardous drug exposure. This product catalog contains information regarding the different
More informationImproving intravenous injection in black mice
Improving intravenous injection in black mice Juerg Messer Roche Pharma Research & Early Development, Neuroscience, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, 4070
More informationTechniques for Biotechnology!
Techniques for Biotechnology! Quantities for Molecular Biology: unlike other labs where we might measure volume in milliliters and mass in grams, in molecular biology / biotech labs, we use MUCH smaller
More informationAccessories / Screwlocks
3401-022 Accessories / Screwlocks Product Features Male or female screwlocks Residual magnetism level - Brass parts: NMB (200 Gamma max) - Stainless Steel parts: NMB (200 Gamma max) 1 part number for 1
More informationAdoptive Transfer of Isolated Bone Marrow Neutrophils Jimena Tosello Boari 1 and Eva Acosta Rodríguez 2*
Adoptive Transfer of Isolated Bone Marrow Neutrophils Jimena Tosello Boari 1 and Eva Acosta Rodríguez 2* 1 Bioquímica Clínica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina
More informationSOP: Blood Collection in the Mouse, Tail Vein
SOP: Blood Collection in the Mouse, Tail Vein These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to investigators
More informationCHARTING THE DEVELOPMENT OF A TRUE DIFFERENTIATED PORTFOLIO OF PARENTERAL DELIVERY SYSTEMS
CHARTING THE DEVELOPMENT OF A TRUE DIFFERENTIATED PORTFOLIO OF PARENTERAL DELIVERY SYSTEMS In this article, Alan Shortall, Chief Executive Officer, Unilife Corporation, describes how the company has developed
More informationSUBJECT: INTRAVENOUS SOLUTIONS AND ADMIXTURE I.V.SOLUTIONS
COALINGA STATE HOSPITAL NURSING POLICY AND PROCEDURE MANUAL SECTION - Medications POLICY NUMBER: 519 Effective Date: August 31, 2006 SUBJECT: INTRAVENOUS SOLUTIONS AND ADMIXTURE I.V.SOLUTIONS 1. DEFINITION:
More informationTREE I.V. Micro-Infusion System
TREE I.V. Micro-Infusion System Training Manual Revision Date: 07/24/15 Subject Headings Table of Contents Pages Introduction: TREE I.V. Micro-infusion 3 Parts of the TREE I.V. Kit 4 3 Easy Steps for the
More informationThe neov endovascular
The neov endovascular Surgical power was never so small The neov surgical laser platform with your choice of 1470 nm or 980 nm - The smallest, lightest weight, and most portable laser system of its power
More informationConstruction. Injectable Hose for Sealing Construction Joints in Watertight Structures. Product Description. Tests. Product Data
Construction System Product Data Sheet Edition: 03/09/2013 Identification no: 02 07 03 07 010 2 000099 Version: GCC Injectable Hose for Sealing Construction Joints in Watertight Structures Product Description
More informationPlastCoat PC 15. The compact one. Interior and exterior plaster spraying with easy to use technology.
PlastCoat PC 15 The compact one Interior and exterior plaster spraying with easy to use technology. With their plaster spraying units Wagner have made it easier to get into the machine-assisted application
More informationDEVELOPMENT AND PRODUCTION OF DIAGNOSTIC CONSUMABLES
OF DIAGNOSTIC CONSUMABLES facilities in the USA, UK, Czech Republic, India and China. clean and well organised, operating to industry certifications with classified clean rooms and bioburden controls available
More informationTSK LABORATORY EUROPE B.V. Gemullehoekenweg CD, Oisterwijk The Netherlands. +31 (0)
TSK LABORATORY EUROPE B.V. Gemullehoekenweg 42 5062 CD, Oisterwijk The Netherlands +31 (0) 85 760 7630 europe@tsklab.com www.tsklab.com Needle Innovations brochure 2016. All rights reserved. Copyright
More informationOrbital welding (Closed head)
5 Orbital welding (Closed head) Our range of closed welding heads (SATF series) allows you to make very high quality welds in a minimum space. These heads have been studied to meet the most stringent welding
More informationEBI 100 Precision Data Loggers
36 EBI 100 Precision Data Loggers With the EBI 100 data loggers, ebro offers reliable measurement devices for routine control according to German guidelines and ISO 15883. The precision data loggers are
More information+91-8048762858 Servo Enterprisess http://www.servoenterprisess.com/ Servo Enterprisess is a leading manufacturer & exporter of Industrial & Scientific Laboratory Equipments. We offer a wide range of products
More informationMid-term review. Recitation5 03/31/2014. Stem Cell Biology and Function W4193 1
Mid-term review Recitation5 03/31/2014 Stem Cell Biology and Function W4193 1 What to expect in the midterm2 It will be all so far but mainly whatever was NOT covered by the previous mid-term- not a huge
More informationInstitutional Animal Ethics Committee Animal House, School of Biological Sciences, NISER, Bhubaneswar. Summary Sheet (To be filled by Investigator)
Institutional Animal Ethics Committee Animal House, School of Biological Sciences, NISER, Bhubaneswar Name of the Principal Investigator: Designation: Summary Sheet (To be filled by Investigator) Contact
More informationterminal blocks
E B Y E L E C T R O terminal blocks S I N C E 1 9 4 5 The EBY Connection Over 60 Years EBY Electro, Inc. has answered OEM production and service requirements since 1945 by supplying specialized components
More informationImaging Requirements (RTOG 1106/ACRIN 6697) Adam Opanowski, CNMT, PET, NCT, R.T.(N)(ARRT) Imaging Analyst ACR Core Laboratory
Imaging Requirements (RTOG 1106/ACRIN 6697) Adam Opanowski, CNMT, PET, NCT, R.T.(N)(ARRT) Imaging Analyst ACR Core Laboratory Imaging Qualification and Quality Control Patients must be scanned on PET/CT
More informationMERCAPTANS, METHYL-, ETHYL-, and n-butyl- 2542
MERCAPTANS, METHYL-, ETHYL-, and n-butyl- 2542 SH MW: 48.11 CAS: 74-93-1 RTECS: PB4375000 SH 62.13 75-08-1 KI9625000 SH 90.19 109-79-5 EK6300000 METHOD: 2542, Issue 1 EVALUATION: PARTIAL Issue 1: 15 August
More information510(k) Summary. 510(k) Number: K Summary Prepared: August 31, 2017
510(k) Summary 510(k) Number: K162613 Summary Prepared: August 31, 2017 Submitter: Dr. Fred Ma Repro Med System, Inc., D/B/A RMS Medical Products 24 Carpenter Road, Chester, NY 10918 Tel: 845-469-2042
More informationMICHAEL Y. CHEN, M.D., RUSSELL R. LONSER, M.D., PAUL F. MORRISON, PH.D., LANCE S. GOVERNALE, AND EDWARD H. OLDFIELD, M.D.
J Neurosurg 90:315 320, 1999 Variables affecting convection-enhanced delivery to the striatum: a systematic examination of rate of infusion, cannula size, infusate concentration, and tissue cannula sealing
More informationCOLLECTION OF SMALL AMOUNTS OF BLOOD FROM TAIL TIP MICROSAMPLING IN MICE
UBC Animal Care Guidelines SOP: ACC 2014 Tech12 microsampling in mice. Submitted by: Shelly McErlane Last date revised: Date approved: June 4, 2014 COLLECTION OF SMALL AMOUNTS OF BLOOD FROM TAIL TIP MICROSAMPLING
More informationPlastCoat PC 25/ PC 35
PlastCoat PC 25/ PC 35 The powerful ones Plaster spraying indoors and out with equipment geared to practical use. Quality Offensive! With their plaster spraying units WAGNGER have made it easier to get
More informationValveless, Ceramic, Chemical Metering Pumps for Precision Low & Micro-Volume Fluid Control
25 September 2012 Pumps & Systems Magazine Herb Werner By Herb Werner Marketing Manager at Fluid Metering, Inc. Valveless, Ceramic, Chemical Metering Pumps for Precision Low & Micro-Volume Fluid Control
More informationWide Range Portfolio. Innovation. Product catalog. Customization. Manufacturing & Assembly
Wide Range Portfolio Product catalog Innovation Customization Manufacturing & Assembly About us E3D specializes in developing and manufacturing auto-injectors and other drug delivery devices for conventional
More informationSEMPEO SQA Unit Code H Carrying out pipe fitting activities
Overview This standard covers a range of basic pipe fitting competences that will prepare you for entry into the engineering or manufacturing sectors, creating a progression between education and employment,
More informationProtocol Form Update 2016
Protocol Form Update 2016 Updated Protocol Form 2016 Updated forms footer: 2016 Minor word changes/layout changes for clarification Major updates to Assurances/Narrative and Training & Qualifications documentation
More informationMill, Burr STANDARD OPERATING PROCEDURE
STANDARD OPERATING PROCEDURE Mill, Burr Model: DK-15LS Manufacturer: Mahlkonig Gmbh & Company KG/Mahlkonig USA Location: Dry Processing Pilot Plant, 1851 Food Sciences Building Publication Date: 04/30/2015
More informationSupporting Information
Supporting Information hoi et al. 1.173/pnas.1187918 SI Materials and Methods Histology. Mice were killed under deep anesthesia by transcardial perfusion with PS, followed by fixation with 4% formalin
More informationPERFUSION TECHNOLOFISTS OF GREATER CHICAGO, INC. POLICY AND PROCEDURE MEDTRONIC HEMOTEC ACTIVATED CLOTTING TIME (ACT) OPERATING PROCEDURE
DATE OF REVISION Page 1 of 13 (ACT) OPERATING PROCEDURE PRINCIPLE: The ACT test is a definitive test used to monitor the anticoagulant effect of heparin. It measures the clotting time of fresh whole blood
More information2.1 This procedure provides steps and conditions for the determination of Vapor Space Organic Compounds in ground waters, and industrial waste waters.
1. TITLE: MSD Analytical Method for Vapor Space Organics 2. PURPOSE: 2.1 This procedure provides steps and conditions for the determination of Vapor Space Organic Compounds in ground waters, and industrial
More informationANIMAL ETHICS APPLICATION GUIDANCE FOR FASTER ANIMAL ETHICS APPROVAL
ANIMAL ETHICS ANIMAL ETHICS APPLICATION GUIDANCE FOR FASTER ANIMAL ETHICS APPROVAL This Animal Ethics Application Guidance document should be used as a companion document whilst completing an Animal Ethics
More informationIsolation of Resting B Lymphocytes from Sixteen Mouse Spleens AfCS Procedure Protocol PP Version 1, 02/19/02
AfCS Procedure Protocol PP00000016 Version 1, 02/19/02 This procedure describes the isolation of resting B lymphocytes (B cells) from mouse spleens by using negative selection with anti-cd43 and anti-mac-1/cd11b
More informationASE SYSTEMS. EPOXY RESIN FLOOR Prep / REPAIR SYSTEM For Air Caster Load Moving Equipment
ASE SYSTEMS The Ergonomics & Productivity Specialists! 5611 Guhn Road Ste: A1 * Houston Texas 77040 *al@asesystems.com 713-462-3072 * fax: 713-462-3540 * www.asesystems.com The following Epoxy Resins &
More information168 West Main Street, #922, New Market, Md Revision 5.2 6/6/2017
QUICK START GUIDE 168 West Main Street, #922, New Market, Md. 21774 TABLE OF CONTENTS Introduction... 2 General Culture Guidelines... 3 Reservoir Bottle and Cap Assembly Sterilization... 4 Pre-culture...
More informationFast Preparative Column Liquid Chromatography (PCLC)
Fast Preparative Column Liquid Chromatography (PCLC) Application Note 224 Joan Stevens, Ph.D. and Gary Scharrer Introduction Preparative HPLC is recognized as a prime method for obtaining pure compounds
More informationSECTION D.3 CHLOROPHYLL AND PHEOPHYTIN
SECTION D.3 CHLOROPHYLL AND PHEOPHYTIN 1.1 Scope and Application 1.1.1 This spectrophotometric method is used in the determination of chlorophyll a, b, and c, and pheophytin a. This method can be used
More informationEliminating Infusion Confusion
Eliminating Infusion Confusion (Drug Administrations in Non-facility Settings) Maryann C. Palmeter, CPC, CENTC Agenda Review of CPT codes (added in 2009) Documentation principles Key definitions What s
More informationDIFFERENTIATION WITHOUT DISRUPTION
DIFFERENTIATION WITHOUT DISRUPTION In this article, Alan Shortall, Chief Executive Officer, Unilife Corporation, describes a new paradigm now driving the pharmaceutical market for advanced drug delivery
More informationRevision 5.1 4/17/17
QUICK START GUIDE 905 West 7th Street # 334, Frederick, MD 21701 TABLE OF CONTENTS Introduction... 2 General Culture Guidelines... 3 Reservoir Bottle and Cap Assembly Sterilization... 4 Pre-culture...
More informationReducing Pre-analytical Errors
Reducing Pre-analytical Errors Christopher R. McCudden, Ph.D., FACB, FCACB, DABCC University of Ottawa The Ottawa Hospital Eastern Ontario Regional Laboratory Association Ontario, Canada Objectives List
More informationS3A:Note for guidance on toxicokinetics: The assessment of systemic exposure in toxicity studies. Q&A: FOCUS ON MICROSAMPLING
S3A:Note for guidance on toxicokinetics: The assessment of systemic exposure in toxicity studies. Q&A: FOCUS ON MICROSAMPLING (Step 2b draft) International Council for Harmonisation of Technical Requirements
More informationMETHOD 310A - DETERMINATION OF RESIDUAL HEXANE THROUGH GAS CHROMATOGRAPHY
METHOD 310A - DETERMINATION OF RESIDUAL HEXANE THROUGH GAS CHROMATOGRAPHY 1.0 SCOPE AND APPLICATION 1.1 This method is used to analyze any crumb rubber or water samples for residual hexane content. 1.2
More informationDetecting Bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets Using Plug-based Microfluidics
Supplemental Material for Detecting Bacteria and Determining Their Susceptibility to Antibiotics by Stochastic Confinement in Nanoliter Droplets Using Plug-based Microfluidics James Q. Boedicker, Liang
More informationSOP 2 Solid Tumor Processing for Cell Culture and Xenografts
Texas Cancer Cell Repository Cell Culture and Xenograft Repository http://cancer.ttuhsc.edu www.txccr.org www.cogcell.org SOP 2 Solid Tumor Processing for Cell Culture and Xenografts Receiving the Sample:
More informationBio Reactor Components from
Bio Reactor Components from Fully stocked and ready to ship! Available in 2, 3, 5, 7 and 15L Sizes Like-for-like replacement parts for Applikon Biotechnology and Broadley-James brands of bio reactors.
More informationPage 1 of 5. Product Name Label Quantity Product No. Cy 3 10 µg (~0.75 nmol) MIR Cy µg (~7.5 nmol) MIR 7901
Page 1 of 5 Label IT RNAi Delivery Control Product Name Label Quantity Product No. Cy 3 10 µg (~0.75 nmol) MIR 7900 Label IT RNAi Delivery Control Cy 3 100 µg (~7.5 nmol) MIR 7901 Fluorescein 10 µg (~0.75
More informationCompany : Medica Middle East Medica Middle East Vision : Medica Middle East Mission : Medica Middle East Medica Middle East Medica Middle East
MME Company Profile Company : Medica Middle East is licensed as a Privet Free Zone Entity to facilitate its importing and exporting activities. Medica Middle East is a subsidiary of THIMAR Holding Group
More information2000-SG STAINLESS STEEL SLIDE GATES
2000-SG STAINLESS STEEL SLIDE GATES 1. GENERAL CONDITIONS 1.1. SCOPE. This section covers Stainless Steel Flow Control Slide Gates and operators. 1.2. GENERAL. Slide gates, including actuation devices,
More informationLeaf-inspired Artificial Microvascular Networks (LIAMN) for Threedimensional
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Supporting Information Leaf-inspired Artificial Microvascular Networks (LIAMN) for Threedimensional
More informationConstruction. Injectable hose for sealing construction joints in watertight structures. Product Description. Product Data. Tests.
System Product Data Sheet Edition 09/01/2009 Identification no: 02 07 03 07 010 2 000099 ( SikaFuko Eco 1 Injectable hose for sealing construction joints in watertight structures Construction Product Description
More informationHuman DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure
Human DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure *Polymerase Chain Reaction is covered by patents owned by Hoffmann-La Roche, Inc. This experiment was adapted from Laboratory
More informationSampling Instructions for Active Sampling
Sampling Instructions for Active Sampling Please read carefully before sampling CASSEN CASSEN Testing Laboratories Telephone: 416 679 9663 Toll Free: 1 866 423 3001 www.cassen.ca CASSEN Testing Laboratories
More informationIntravitreal and sub-retinal injections of plasmid DNA and electroporation in P0 pups
Intravitreal and sub-retinal injections of plasmid DNA and electroporation in P0 pups Protocol modified from: Retinal Gene Delivery by raav and DNA Electroporation, Aditya Venkatesh et all, Current Protocols
More informationSupplementary Information
Supplementary Information Table S1: Microfabrication process overview Layer Height Resist RPM P, 65 S, 95 Exposure H, 95 DW channels 15µm 2015 3300 1 min 3 min 150 sec 4 min chaotic mixers 5µm 2005 3000
More informationSDS-PAGE GEL. Bio-Rad EDU Cell scraper TPP PBS Life Technologies Protein Standard Ladder Bio-Rad
Institut für HIV Forschung SOP#11-00 (Aug 2017 - BS) SDS-PAGE GEL Reagent Manufacturer Order Number 5M NaCl Solution Sigma S5150 Dithiothreitol (DTT) Sigma 10197777001 HEPES Sigma H0887-100ml Sodium dodecyl
More informationDetermining the Density of Unknown Substances
Determining the Density of Unknown Substances Introduction: Most of the elements on the periodic table are metals and solids. These elements have observable properties that make it possible to identify
More informationBt-Cry1F ELISA Kit Qualitative DAS ELISA for the detection of the Bt-Cry1F transgenic protein Catalog number: PSP 10301
List of contents Lot number Item 96 wells 288 wells 480 wells 4800 wells Antibody-coated 96-well microtiter plates 1 3 5 50 solid Peroxidase enzyme conjugate (1x, ready to use) 11 ml 33 ml 55 ml 550 ml
More informationSOP: Blood Collection in the Mouse, Retro-orbital Bleed
SOP: Blood Collection in the Mouse, Retro-orbital Bleed These SOPs were developed by the Office of the University Veterinarian and reviewed by Virginia Tech IACUC to provide a reference and guidance to
More informationPart 4: Prefilled syringes
INTERNATIONAL STANDARD ISO 11040-4 Third edition 2015-04-01 Prefilled syringes Part 4: Glass barrels for injectables and sterilized subassembled syringes ready for filling Seringues préremplies Partie
More informationPrepFiler Express and PrepFiler Express BTA Forensic DNA Extraction Kits
QUICK REFERENCE CARD PrepFiler Express and PrepFiler Express BTA Forensic DNA Extraction Kits Note: For safety and biohazard guidelines, refer to the Safety section in the PrepFiler Express and PrepFiler
More information