This week. Outline. How to get doubling time from graph. For ex 8:
|
|
- Walter Webster
- 6 years ago
- Views:
Transcription
1 Outline This week s plans Growth curve analysis Thursday Introduction to the food lab Introduction to Kirby Bauer and Antibiotics Group projects--hand back of feedback, revised versions due Thursday 1 This week Tuesday: Bring in food sample and perform food lab (ex. 9) Carry out cross streak with soil microbe Streak Nitrogen-free enrichments Thursday: Analysis of antibiotics using Kirby Bauer Count food lab plates Examine soil/cross streak plates Revised group project due Growth curve lab due (ex 8) 2 For ex 8: 1. Present your results as (1) Table of OD 550 readings at each time; (2) Semi-log plots constructed by hand; (3) Hand-fitted slopes of your plots. Do both plus and minus antibacterial agent (can be on same graph). You must do it by hand, but can also do it by excel. The slope = generation or doubling time 2. Include one-two paragraphs discussing your results that includes the two generation times (both plus and minus antibacterial). Also discuss why you got your specific outcome, including how your antibacterial compound functions. Discuss how you think your antibacterial should affect OD550 and compare that to your actual data. Be sure to include references for where you got your information about the compound. How to get doubling time from graph Culture must be in exponential growth phase to only take the points from that part Plot the log of the cell densities time Slope will give the generation or doubling time Can determine by just examining graph 3. Perform a Pubmed search for your antibacterial, and then do a second one comprised of your antibacterial+a bacterium you are interested in. Select one paper, and summarize the findings in one of the figures or tables. Include your search terms
2 Tuesday we will analyze food for microbes Each group of four needs to bring in a food, don t forget! You need 20 grams. Can drop it off (label the bag) in the classroom fridge Foodborne disease Food-associated infectious agents E. coli O157:H7, Campylobacter, Salmonella Usually proper cooking will kill these agents Agents that multiply in improperly stored food. Some may produce toxins that cause the illness. Listeria, Staphylococcus, Clostridia botulinum 5 6 Many of these agents are covered by FDA testing requirements E.coli O157:H7 Genetically different from lab E. coli but also gramnegative, motile, rod Has extra set of genes Got it s name from a typing scheme 1) In this scheme, researchers use antibodies to highly variable surface antigens. They have a bank of these antibodies, and basically just say that a given strain reacts with, for example, Antibody #26. 2) Two antigens are tested a) one is the O Antigen, aka LPS b) one is the H Antigen, aka Flagellin» O157 means it has O-antigen #157» H7: means it has flagellum #7 Also called EHEC, for Enterohemoragic E. coli 7 8 2
3 EHEC Disease You ingest bacteria in contaminated food Has a very low infectious dose of ~ Bacteria gets to your intestine and multiply. You get abdominal cramping, nausea, bloody diarrhea Other E. coli s cause diarrhea, but usually it is not bloody 3. Bacteria Adhere tightly to your cells The type of adherence looks similar to another E. coli, called EPEC which is better studied (these notes are based on EPEC) Cup-like pedestal under the E. coli. Attaching and Effacing. Adhering bacteria can deliver proteins directly to the host cell 9 EHEC Disease 2 4. EHEC Produces a Toxin called VeroToxin Being able to produce this toxin is the main difference between EPEC and EHEC Highly related to Shiga Toxin (STX) from Shigella Protein Toxin that is made by E. coli in the intestine but gets into the blood, and can travel to the kidneys Cleaves host cell ribosomal RNA and stops protein synthesis Our cells have receptors for Verotoxin, but only intestinal and kidney cells Kidney disease is called Hemoyltic Uremia Syndrome gene for verotoxin is carried by a temperate bacteriophage integrated into the genome. Can be passed readily from strain-strain; EHEC probably was created when an EPEC acquired the phage from a Shigella 10 EHEC is a commensal of cattle Recent EHEC Outbreaks FIG. 2. Mean counts of tissue-associated E. coli O157:H7 on five regions of the rectum defined by distance from the RAJ (in centimeters). Error bars indicate upper and lower 95% confidence intervals. From Infection and Immunity, March 2003, p , Vol. 71, Lymphoid Follicle-Dense Mucosa at the Terminal Rectum Is the Principal Site of Colonization of Enterohemorrhagic Escherichia coli O157:H7 in the Bovine Host. Naylor, et al. Resides in region of intestine called rectoanal junction Is shed in feces, and also ends up on hair Current strategy is to remove coat and clean the carcass in a separate area from subsequent processing Cook meat well 11 Many foods can be contaminated--beef, cookie dough, spinach. On-going Multistate Outbreak of E. coli O157:H7 Infections Associated with Beef from National Steak and Poultry aks.html There are testing standards but it is challenging when infection can be by 10 organisms. Typically PCR-based No major outbreaks recently, although several smaller ones. Links on web page 12 3
4 Food Lab-Overview Each group of four is bringing in food item You will need 20 g, which is about 20 ml of a wet food, or ~ 1 ounce Try to minimize hand exposure Food Lab-safety Blender food up in the biosafety cabinet Will have sign up sheet to make it all go smoothly After blendering, wait a few minutes before you open up the blender to let aerosols sink We will plate the blended food in two ways: Mix with molten nutrient agar to disperse the bacteria througout the agar Streak some of the food onto EMB agar (same as used for water lab) to look for E. coli Microbiological analysis of food Goal is to calculate the CFU/g g ml ml 99 ml g Food Lab-data analysis Lab Report Due 11/ ml ml 99 ml 0.1 ml 1 ml 0.1 ml The 10 4 plate has: 10 CFU/0.1 ml 100 CFU/ml This sample was diluted by 100: 100 CFU*100 = 10 4 CFU/ml There was 200 ml total in this bo7le 0.1 ml 1 ml 0.1 ml 100 CFU 10 CFU Slide courtesy Chad Saltikov 10-2 plate 10-3 plate 10-4 plate 10 4 CFU/ml * 200ml = 2x10 6 CFU/bottle 20 g of food/bo7le 2x10 6 CFU/bottle*(bottle/20g) = 1x10 5 CFU/g 16 4
5 Soil Lab: Azotobacter enrichment results Soil Lab: clearing-zone colony follow up Examine Azotobacter flask for gummy growth Streak gummy growth onto Azotobacter plates for single colonies Cross streak Tuesday Examine Thursday 17 Kirby-Bauer Analysis of response to antibacterial compounds We need overnight grown cultures for Thursday so Weds before 5 pm: Head to the lab. Plates will be set up on 229, labeled UNKNOWN 1, 2, etc Select two single colonies from two different plates Inoculate each one into a tube of sterile broth, using loops or sterile sticks Place tubes in 37ºC incubator with shaking on (same one as used for Water durham tubes) Each group should have two tubes Kirby Bauer Swab each of your cultures + E. coli control onto Mueller Hinton Agar Add antibiotics to each sterile disk and place disk on plate Template at end of exercise to help with placing discs. Need all 8 on each plate. Incubate until Next day at 37ºC (staff will grab all plates) X / jpg 20 5
6 Antibiotics target crucial bacterial processes Kirby Bauer: Results Measure and record diameter of clearing zone Note whether zones are totally or partially clear, or even contain small colonies that may be resistant mutants 21 New Antibiotics are needed: 1) Resistance 2) More choices Antibiotic Resistance is serious 24 6
7 New Antibiotics Almost all antibiotics come from microbes Streptomyces spp: streptomycin, kanamycin, other mycins, tetracycline Penicllium molds produce penicillins Search for new includes Chemically synthesizing variants/analogs Random screening of chemicals Brock quotes that 7 million chemicals have to be screened to identify one useful drug, lasting years from start to approval. Computer-aided design to predict small molecules that bind to targets HIV protease inhibitors Random screening of natural products Bacteriophages 25 Example: Platensimycin Found in a screen of 250,000 natural product extracts from 83,000 potential antibiotic producing strains at Merck Screened against Staphylococcus aureus, but started with a crippled strain that had a defect in lipid synthesis Goal was to get an antibiotic that targeted this pathway as that would be unique Crippled the strain using anti-sense RNA against a known enzyme in the pathway to basically make the strain more sensitive to a second block on this pathway Review article on web site, in readings portion High throughput screening here at UCSC 26 Example: Daptomycin/Cubicin Found recently by screening soil samples, new antibiotic that makes membranes lose their PMF. Works against gm-positives like Staph. Group projects Specific feedback handed back today; revision due Thursday Thursday revision should include a complete materials lists for everything: Amount of media, recipes Other things like swabs Equipment like shakers, blenders, specs
Outline. This week s plans Introduction to Kirby Bauer and Antibiotics Introduction to Biochemical analysis of strains
Outline This week s plans Introduction to Kirby Bauer and Antibiotics Introduction to Biochemical analysis of strains 1 This week 2 Monday Finish Exercise 10, part C. Run MspI restriction digest on a gel
More informationDiscussion Items. Microbial Indicators of Water Quality
Discussion Items! Announcements! Group Project topics! Discuss previous lab (Microbes in food) Results Lab report Isolates streak isolate by Thursday.! Microbial analysis of water (Tuesday) MPN and MF!
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationToday s Lecture. Review of events for this week Background material so you can understand DNA extrac?on and PCR Quiz
Today s Lecture Review of events for this week Background material so you can understand DNA extrac?on and PCR Quiz This week Con?nue purifying colonies and start a liquid overnight culture Extract DNA
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationSYNTHETIC BIOLOGY AND THE HIGH SCHOOL CURRICULUM: LAB 1 _Lab_1
SYNTHETIC BIOLOGY AND THE HIGH SCHOOL CURRICULUM: LAB 1 http://openwetware.org/wiki/synthetic_biology_and_the_high_school_curriculum: _Lab_1 LAB 1: Eau that smell Comparing 2 competing designs to optimize
More informationNATIONAL FOOD SAFETY FOUNDATION
NATIONAL FOOD SAFETY FOUNDATION RAPID TESTING KITS DETECTION E.COLI 0157 IN FOOD MATICES AND LIQUIDS USING E.COLI RAPID TESTING KIT INTRODUCTION E. coli serotype O157 is a gram-negative rod-shaped bacterium.
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationLab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic
Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative
More informationLAB 1: Eau that smell
LAB 1: Eau that smell Compare 2 competing designs to optimize system performance Acknowledgements: This lab was developed with materials and guidance from the MIT 2006 igem team, as well as technical insights
More information01/08/2018. Control of Microbial Growth. Methods. Terminology. Disinfectants and Antiseptics. Three approaches. Cleaning. Chemical.
Control of Microbial Growth Disinfectants and Antiseptics 1 Methods 2 Three approaches Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical elimination Cleaning Filtration
More informationProject 5: Urine Cultures and Identification
Project 5: Urine Cultures and Identification Readings: http://www.webmd.com/a-to-z-guides/urine-culture http://www.medscape.com/viewarticle/558845 (Listen to the two lectures by Dr. Robert A. Weinstein.)
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationAdapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692
Biology 4B Laboratory Bacteriological Examination of Water Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Objectives Carry out
More informationExercise 23-C BACTERIOPHAGE REPRODUCTION AND PLAQUE FORMATION
Exercise 23-C BACTERIOPHAGE REPRODUCTION AND PLAQUE FORMATION Introduction The reproductive cycle of a cytolytic bacteriophage called T2 begins with its adsorption onto a sensitive host cell. The phage
More informationTransduction of an Antibiotic Resistance Gene. Background
I Student Guide 21-1128 Name------------ Date Transduction of an Antibiotic Resistance Gene Background Transduction is a natural method of gene transfer that occurs in bacteria. The key player in transduction
More informationLab Three :. Sensitivity test:
Lab Three :. Sensitivity test: Or Diffusion Test: Antibiotic sensitivity test: is a laboratory method for determining the susceptibility of organisms to therapy with antibiotics, Antibiotic susceptibility
More informationBiology (Microbiology): Exam #3
NAME: PLEDGE: Biology 50-384 (Microbiology): Exam #3 1. You have isolated a series of mutants that have altered patterns of ß-galactosidase and lactose permease activity (i.e. they are different that the
More informationBiology 322 Fall 2010 Transfer of genetic information in the bacterium Escherichia coli: Part I
Biology 322 Fall 2010 Transfer of genetic information in the bacterium Escherichia coli: Part I REQUIRED Reading Assignments: Superbugs on the Hoof http://fire.biol.wwu.edu/trent/trent/superbugs.pdf Triple
More informationPenicillin Streptomycin
BTEC 4200 Name Fall 2005 Exam 2 A. Multiple choice (2 pt each) The following choices are used for questions 1 5. Trypan red Arspheniamine (Salvarsan) Sulfonamide Penicillin Streptomycin 1. This substance,
More informationShehab. Yousef... Omar. Yousef Omar. Anas
3 Shehab Yousef Omar Yousef... Omar Anas Bacterial Growth and Survival After discussing the structure of a Bacteria, we must know how it survive and grow in a specific media. Firstly, the survival of any
More informationPr oject Summar y. Rapid quantification of culturable and viable-but-nonculturable Escherichia coli O157:H7 in beef products using EMA-Real Time PCR
Pr oject Summar y Rapid quantification of culturable and viable-but-nonculturable Escherichia coli O17:H7 in beef products using EMA-Real Time PCR Principal Investigator: Azlin Mustapha University of Missouri
More informationENVIRONMENTAL PARAMETERS OF GROWTH
ENVIRONMENTAL PARAMETERS OF GROWTH The growth and survival of microorganisms are affected by the chemical and physical conditions of the external environment. Environmental factors which have significant
More informationBIOLOGY. Bacteria Growth Lab. Bacterial Growth. Slide 2 / 61. Slide 1 / 61. Slide 4 / 61. Slide 3 / 61. Slide 5 / 61. Slide 6 / 61
Slide 1 / 61 Slide 2 / 61 New Jersey Center for Teaching and Learning Progressive Science Initiative This material is made freely available at www.njctl.org and is intended for the non-commercial use of
More informationMicrobial Survival. Created on 2/25/ :05 AM
Microbial Survival Introduction The main objectives of this practical are: Verify, or otherwise, the Department of Health recommendation that food be cooked to 70 o C for 2 minutes or an equivalent time-temperature
More informationE. coli O157 Test Strip Kit Catalog #: 1062
BIOO FOOD AND FEED SAFETY E. coli O157 Test Strip Kit Catalog #: 1062 BIOO Scientific Corp. 2011 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview... 1 Kit Contents,
More informationKEY. Biology Baseline Cornerstone Assessment: Part A. Experimental Design
Biology Baseline Cornerstone Assessment: Part A. Experimental Design Directions: Read the paragraph below and then respond to the questions. Students in a biology class were discussing outbreaks of food-borne
More information1. Procedure for Antibiotic susceptibility test by disc diffusion analysis
Nanoparticles Functionalized with Ampicillin Destroy Multiple Antibiotic Resistant Isolates of Pseudomonas aeruginosa, Enterobacter aerogenes and Methicillin Resistant Staphylococcus aureus Ashley Brown
More informationAntibiotic Susceptibility Testing (ABST/AST)
Antibiotic Susceptibility Testing (ABST/AST) Goal Offer guidance to physicians in selecting effective antibacterial therapy for a pathogen in a specific body site. Performed on bacteria isolated from clinical
More informationEscherichia coli O157:H7 (E.coli O157:H7) ELISA
Product information User s Manual Escherichia coli O157:H7 (E.coli O157:H7) ELISA Enzyme-linked immune-sorbent assay for the determination of E.coli O157:H7 in food or water. Catalog No.: BE69221 96 Storage:
More informationAdvances in detecting pathogens in foods. Roy Betts Head of Microbiology
Advances in detecting pathogens in foods Roy Betts Head of Microbiology Test Methods - why are they important? Everything we know about the microbiology of our food is based on testing We make very big
More informationBacterial Plate Preparation. ~ Using aseptic techniques ~
Bacterial Plate Preparation ~ Using aseptic techniques ~ Bacterial Plates Laboratory and research scientists have to prepare nutrient media to grow specific strains of bacteria for their research. To do
More informationLAB NOTES FOR EXAM 1 SECTION
LAB NOTES FOR EXAM 1 SECTION EX. 2-1: DIVERSITY AND UBIQUITY OF MICROOGANISMS Purpose: Microorganisms are found everywhere in the environment around us. To demonstrate this and to get a taste of the different
More informationINTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants
INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly
More informationMICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA
MICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA When we receive a sample (ex. Urine sample) for detection, we cannot gram stain it right away if it requires to be inoculated because when
More informationHeat Shock Proteins in Yeast (2012)
MASSACHUSETTS INSTITUTE OF Technology Department of Biology Heat Shock Proteins in Yeast (2012) Summary Lydia Breen (Stoneham High School) Mary Brunson (Brookline High School) Yeast is a single-celled
More informationMiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3. Introduction
MiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3 Introduction This unit uses the development of resistance to antimicrobials as an example of natural selection. The students
More informationA NEW METHOD FOR THE DEVELOPMENT OF ESCHERICHIA COLI RESISITANT AGAINST ANTIBIOTIC AMPICILLIN
A NEW METHOD FOR THE DEVELOPMENT OF ESCHERICHIA COLI RESISITANT AGAINST ANTIBIOTIC AMPICILLIN Richa Thakre*, Shahbaz Eqbal, Abhishek Dwivedi and Shilpa Jain 1 NRI Institute of Pharmaceutical Science, Bhopal,
More informationChapter 10 Microbial Genetics: New Genes for Old Germs
Chapter 10 Microbial Genetics: New Genes for Old Germs Objectives: After reading Chapter Ten, you should understand The structure and complexity of the bacterial chromosome and the significance of plasmids.
More informationMick Bosilevac 1, Brandon Leudtke 1 Rong Wang 1 and Yemi Ogunrinola 2. US Meat Animal Research Center, Clay Center NE
Development and Application of a Green Fluorescent Protein (GFP) Expressing E. coli O103 Surrogate for Tracking Contamination Through Grinding and Identifying Persistent Points of Contamination Mick Bosilevac
More informationBACTERIAL GENETICS: Labs I & II
BACTERIAL GENETICS: Labs I & II The Bacterial Genetics Labs will extend over two laboratory periods. During the first lab, you will set up two different experiments using the bacterium Escherichia coli.
More informationnumber Done by Corrected by Doctor
L number Lab 2 Done by حسام أبو عوض Corrected by Mahdi sharawi Doctor In many cases we need to identify the type of bacteria causing an infection in order to be able to choose the right medication (antibiotic).
More informationMicrobiology sheet (6)
Microbiology sheet (6) Made by marah marahleh corrected by : abd. Salman DATE :9/10/2016 Microbial growth / control of microbial growth 1 The method of counting bacteria is divided into: 1) direct 2) indirect
More informationCell Growth and DNA Extraction- Technion igem HS
Growing Cells and DNA Extraction Goals 1. Become familiar with the process of growing bacteria 2. Get to know the DNA extraction process 3. Perform miniprep in the lab Keywords 1. Growth stages 6. Techniques
More informationAseptic Techniques. A. Objectives. B. Before coming to lab
Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note
More informationPuritan Environmental Sampling Kit (ESK )
Puritan Environmental Sampling Kit (ESK ) Table of Contents: Introduction Pg.2 Description..Pg.2 Production Information.... Pg.2-4 Attributes and Intended Uses... Pg.4-5 Common Food and Water Borne Pathogens...Pg.5-6
More informationCarriage and control of verocytotoxigenic E. coli in cattle. Geraldine Duffy, Teagasc Food Research Centre
Carriage and control of verocytotoxigenic E. coli in cattle Geraldine Duffy, Teagasc Food Research Centre Overview Verocytoxigenic E. coli : an evolving pathogen VTEC carriage in cattle and shedding patterns
More informationBACTERIAL CONJUGATION. To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another.
BACTERIAL CONJUGATION I. OBJECTIVES To demonstrate the technical procedure to monitor the conjugational transfer of genetic material from one cell to another. To learn about the various genetic elements
More informationRIDA Anreicherungsbouillon
RIDA Anreicherungsbouillon mtsb for the enrichment of shigatoxin-producing E. coli bacteria Article no: Z1000 Article no: Z1003 R-Biopharm AG, An der neuen Bergstraße 17, D-64297 Darmstadt, Germany Tel.:
More informationSection A: Prokaryotes Types and Structure 1. What is microbiology?
Section A: Prokaryotes Types and Structure 1. What is microbiology? 2. Compare and contrast characteristics of each bacterial type: Eubacteria and Archaebacteria. Eubacteria Both Archaebacteria 3. Label
More informationAntibiotics and alternative strategies to control infections
Antibiotics and alternative strategies to control infections http://blog.microbiologics.com/wpcontent/uploads/2015/12/acinetob acter-baumannii-232x300.jpg www.biochemj.org/bj/330/0581/bj3300581.htm ciss.blog.olemiss.edu
More informationActero Salmonella/STEC Enrichment Media Product Information
Actero Salmonella/STEC Enrichment Media Product Information INTENDED USE: Actero Salmonella/STEC Enrichment Media is a selective medium optimized for an improved enrichment of Salmonella spp. from food
More informationBIMM 121 Letter Grade by Practicum. Student Information Sheet
BIMM 121 Letter Grade by Practicum Student Information Sheet BIMM 121 Laboratory in Microbiology is a course that combines intensive training in microbiology and physiology content with training and practice
More informationInside the Burch Lab: E. Coli and Triclosan Resistance. By: Pamela Lammonds
Inside the Burch Lab: E. Coli and Triclosan Resistance By: Pamela Lammonds Purpose and Goals of Research Concerns over infectious disease have risen in the past few years. In response to this concern,
More information7.17: Writing Materials & Methods Spring A Methods Section Exercise
7.17: Writing Materials & Methods Spring 2006 Neal Lerner, nlerner@mit.edu, x2-2939 A Methods Section Exercise 1. Draw a relatively simple picture. 2. Write an account of how you drew that picture. 3.
More informationLab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl)
Lab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl) Some of the diseases that humans can contract from drinking polluted water include typhoid, dysentery,
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationE. coli O26 Latex Test Kit
Importance of STEC Determination Shiga toxin-producing Escherichia coli strains (non-o157 STEC) have become an increasing public health concern. Some of the non-o157 STEC possess the same range of virulence
More informationLab Date Experiment Reports, Midterms, Reminders
Lab Date Experiment Reports, Midterms, Reminders Lab 1 Lab 2 Jan 5-6 Jan 7-8 o Registration, introductory remarks, safety lecture, etc. Sterile technique. o Expt 1: Microbes in the environment o Expt 2:
More informationStudy Title Antibacterial Activity and Efficacy of KHG FiteBac Technology Test Substance Using a Suspension Time-Kill Procedure
Study Title Antibacterial Activity and Efficacy of KHG FiteBac Technology Test Substance Using a Suspension Time-Kill Procedure Test Method ASTM International Method E2315 Assessment of Antimicrobial Activity
More informationPrevalence of Shiga-Toxin Producing Escherichia Coli in Two Cohorts of Beef Cattle is Associated with Diversity of Microflora and Animal Age
Prevalence of Shiga-Toxin Producing Escherichia Coli in Two Cohorts of Beef Cattle is Associated with Diversity of Microflora and Animal Age Raies A. Mir 1,2, Sarah M. Markland 1,2, Mauricio Elzo 1, Soohyoun
More informationENVIRONMENTAL PARAMETERS OF GROWTH
ENVIRONMENTAL PARAMETERS OF GROWTH The growth and survival of microorganisms are affected by the chemical and physical conditions of the external environment. Environmental factors which have significant
More information01/08/2018. Counting Microorganisms. Counting microorganisms. Turbidity Measurements. Relative abundance. Direct counts.
Counting Microorganisms 1 Counting microorganisms 2 Relative abundance Turbidity measurements Direct counts Absolute counts Viable counts Absolute number of growing bacteria Most probale number (MPN) Probable
More informationExploring STEAM with Transformation
Exploring STEAM with Transformation Thomas Cynkar Edvotek www.edvotek.com Follow @Edvotek EDVOTEK Biotech The Biotechnology Education Company Celebrating 30 years of science education! EDVOTEK Educatio
More informationComparison of Detection Methods of non-o157 STEC
Comparison of Detection Methods of non-o157 STEC USDA is an equal opportunity provider and employer Mick Bosilevac, PhD USDA -Agricultural Research Service U.S. Meat Animal Research Center Meat Safety
More informationGROWTH AND SURVIVAL OF PATHOGENIC E. COLI DURING CURDLING OF MILK
Int. J. LifeSc. Bt & Pharm. Res. 2014 Aryya Mitra and Sanjib Ghoshal, 2014 Research Paper ISSN 2250-3137 www.ijlbpr.com Vol. 3, No. 1, January 2014 2014 IJLBPR. All Rights Reserved GROWTH AND SURVIVAL
More informationMACROPHAGE KILLING ASSAY
MACROPHAGE KILLING ASSAY Updated by: Joseph Chon Date: July 2018 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND This protocol is used to determine a macrophage population
More informationReport BerryMeat. Antimicrobial effect for different preparations from 8 plants during storage at 18 C for 1½ year. Flemming Hansen.
Report BerryMeat Antimicrobial effect for different preparations from 8 plants during storage at 18 C for 1½ year. 19. marts 2014 Project 2000248-13 FH/ Flemming Hansen Summary Introduction In the project,
More informationIsolation & Characterization of Bacteria
PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!
More informationInoculate: Media. Physical State of Media: Liquid. The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory
The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory 1. Inoculate 2. Incubate 3. Isolate 4. Inspect 5. Identify The Five I s: Inoculate Inoculate: Media Classified according
More informationGENUS STAPHYLOCOCCUS: Isolation and Identification
GENUS STAPHYLOCOCCUS: Isolation and Identification Staphylococcus is a genus of Gram +, nonspore-forming cocci belonging to the family Micrococcaceae that are often found as normal human microbiota of
More informationLab 5/5a Transformation of E. coli with a Recombinant Plasmid
Lab 5/5a Transformation of E. coli with a Recombinant Plasmid Lab 2 Pre Lab Readiness Familiarity and Proper use of micropipettes Remember the 1 st and 2 nd stops Aseptic Technique Antibiotic Resistance
More informationLab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab
Lab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab OBJECTIVES 1. Understand the use of MPN to determine likely fecal water contamination. 2. Understand the use of MUG,
More informationCHAPTER 24. Immunology
CHAPTER 24 Diagnostic i Microbiology and Immunology Growth-Dependent Diagnostic Methods Isolation of Pathogens from Clinical Specimens Proper sampling and culture of a suspected pathogen is the most reliable
More information2/25/2013. Psychrotrophs Grow between 0 C and C Cause food spoilage Food Preservation Temperatures
3 4 5 6 7 8 9 0 Chapter 6 Microbial Growth Microbial Growth Increase in number of cells, not cell size Populations Colonies The Requirements for Growth Physical requirements Temperature ph Osmotic pressure
More informationPreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about
BIO101Lab weeks 4 and 5 PreLab Activity: Read through the entire lab! Come prepared to explain to your group what this lab is about The Evolution of the bacterium, Pseudomonas fluorescens Introduction
More information6/28/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics
Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical
More informationTransformation: Theory. Day 2: Transformation Relevant Book Sections
Day 2: Transformation Relevant Book Sections We will follow the protocols provided in various industry-standard kits, instead of the protocols described in these chapters, but the chapters provide good
More informationLab Exercise 13: Growth Curve
Lab Exercise 13: Growth Curve OBJECTIVES 1. Know the different phases of a standard growth curve. 2. Understand and perform direct measurement of bacterial growth through serial dilutions and standard
More informationBIMM 121 Learning Goals, Outcomes, Assessments, Practice
BIMM 121 Learning Goals, Outcomes, Assessments, Practice Learning Goals: tudents will: A. General cientific/lab kills and Competencies 1. Learn the importance of designing and using the right controls
More informationBYA3. HUMAN BIOLOGY (SPECIFICATION A) Unit 3 Pathogens and Disease. General Certificate of Education June 2005 Advanced Subsidiary Examination
Surname Other Names Leave blank Centre Number Candidate Number Candidate Signature General Certificate of Education June 2005 Advanced Subsidiary Examination HUMAN BIOLOGY (SPECIFICATION A) Unit 3 Pathogens
More informationFigure 1. Map of cloning vector pgem T-Easy (bacterial plasmid DNA)
Texas A&M University-Corpus Christi CHEM4402 Biochemistry II Laboratory Laboratory 6: Ligation & Bacterial Transformation (Bring your text and laptop to class if you wish to work on your assignment during
More informationConfirming the Phenotypes of E. coli Strains
Confirming the Phenotypes of E. coli Strains INTRODUCTION Before undertaking any experiments, we need to confirm that the phenotypes of the E. coli strains we intend to use in the planned experiments correspond
More informationRequirements for Growth
Requirements for Growth Definition: Bacterial growth defined as an increase in the number of cells. Physical Requirements: temperature, ph, tonicity Temperature: On the basis of growth range of temperature
More informationMicrobiology for Oral and Topical Products - The basics Scott Colbourne Business Manager NSW ALS Food & Pharmaceutical
Microbiology for Oral and Topical Products - The basics Scott Colbourne Business Manager NSW ALS Food & Pharmaceutical RIGHT S O L U T I O N S RIGHT PARTNER Contents TGO 77 - Introduction Tests Performed
More informationEnterohaemorrhagic E. coli assay
Enterohaemorrhagic E. coli assay For the detection of EHEC stx1, EHEC stx2, EHEC eaea and EHEC hlya genes using the BD MAX TM system. Instructions for use (Version 3.0 February 2018) 1 Contents Introduction
More informationSyllabus for Biology 421L and 422L General information Biology 422 has an optional lab. There are two lab sections: a two credit section on Tuesday
Syllabus for Biology 421L and 422L General information Biology 422 has an optional lab. There are two lab sections: a two credit section on Tuesday afternoon taught as Biol 421L and a one credit section
More informationChapter 6: Microbial Growth
Chapter 6: Microbial Growth 1. Requirements for Growth 2. Culturing Microorganisms 3. Patterns of Microbial Growth 1. Requirements for Growth Factors that affect Microbial Growth Microbial growth depends
More informationONTARIO SCIENCE CENTRE. Teacher Guide. Way to Glow Program
ONTARIO SCIENCE CENTRE Teacher Guide Way to Glow Program Table of Contents Bacterial transformation background information 3 Experimental procedure 5 Expected results 7 Post-program activity sheet 8 Post-program
More information10/2/2016. Control of Microbial Growth. Method. Terminology. Disinfectants and Antiseptics
Control of Microbial Growth Disinfectants and Antiseptics 1 Method Three approaches for the control of microbial growth Chemical Disinfectants and antiseptics Physical Heat Ultraviolet Irradiations Mechanical
More informationTest Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002
Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 FTTS-FA-002 Antibacterial Textiles for Medical Use Antibacterial Textiles suppress and even kill harmful bacteria
More informationBACTERIOPHAGES: STRUCTURE AND PROPERTIES OF BACTERIAL VIRUSES
BACTERIOPHAGES: STRUCTURE AND PROPERTIES OF BACTERIAL VIRUSES Bacteriophage (phage) are obligate intracellular parasites that multiply inside bacteria by making use of some or all of the host biosynthetic
More informationTransmission of genetic variation: conjugation. Transmission of genetic variation: conjugation. Transmission of genetic variation: F+ conjugation
Transmission of genetic variation: conjugation Transmission of genetic variation: conjugation Direct transfer of DNA from one strain to another mediated by fertility factor (F). Best studied in E. coli,
More informationMICROBIAL PLATE COUNT USING IMAGE PROCESSING
MICROBIAL PLATE COUNT USING IMAGE PROCESSING By PAWANKUMAR FAKATKAR Department of Electronics & Telecommunication Engineering Sardar Patel Institute of Technology Munshi Nagar, Andheri(W), Mumbai-400058
More informationGenetic Basis of Variation in Bacteria
Mechanisms of Infectious Disease Fall 2009 Lecture 2 Jonathan Dworkin, PhD Department of Microbiology jonathan.dworkin@columbia.edu Genetic Basis of Variation in Bacteria I. Organization of genetic material
More informationCh 6. Microbial Growth
Ch 6 Microbial Growth Student Learning Outcomes: Classify microbes into five groups on the basis of preferred temperature range. Explain the importance of osmotic pressure to microbial growth. Provide
More informationCOMPARITIVE STUDY OF THE EFFECT OF SINGLE WALLED CARBON NANOTUBES ON Escherichia coli IN CULTURES AND BIOFILMS. A write-up on the proposed Study
COMPARITIVE STUDY OF THE EFFECT OF SINGLE WALLED CARBON NANOTUBES ON Escherichia coli IN CULTURES AND BIOFILMS A write-up on the proposed Study Prepared by: Indumathy Jayamani For: Ene 806 Laboratory Feasibility
More informationMMG 301, Lec. 25 Mutations and Bacteriophage
MMG 301, Lec. 25 Mutations and Bacteriophage Questions for today: 1. What are mutations and how do they form? 2. How are mutant bacteria used in research? 3. What are the general properties of bacteriophage
More informationLab 1: Eau that smell
Lab 1: Eau that smell Teacher Considerations This lab provides a valuable opportunity to teach microbiology techniques, population growth dynamics, molecular genetics and basic synthetic biology concepts
More information