QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd

Size: px
Start display at page:

Download "QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd"

Transcription

1 QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd 1

2 Our current NGS & Bioinformatics Platform 2

3 Our NGS workflow and applications 3

4 QIAGEN s solution for targeted DNA sequencing QIAGEN can provide solution for targeted DNA sequencing from DNA isolation to data interpretation 4

5 GeneRead DNAseq Targeted panels (V2) Benefits of GeneRead DNAseq Targeted Panels (V2): Single panel for all NGS platforms Well-suited for FFPE samples Use as little as 10 ng starting DNA per PCR pool Rapid turn-around time (3 hours) Clinically and biologically relevant content: GeneRead DNAseq Targeted Panels focus on specific mutations, exons, and genes that are most relevant to a particular disease. The content of these panels was selected from the College of American Pathologists(CAP) guidelines, NCCN guidelines, late-stage clinical trials, The Cancer Genome Atlas (TCGA), and Ingenuity Knowledge Base. GeneRead DNAseq Targeted Panels workflow 5

6 GeneRead DNAseq Targeted panels (V2) The GeneRead DNAseq Targeted Panels(V2) enable enrichment of the coding regions and exon/intron junctions of genes frequently mutated in a particular disease. Using a high-dimension multiplex PCR targeted enrichment approach, DNAseq targeted panels increase the efficiency of sequencing efforts by focusing on the genes most relevant to a specific disease or research area. Multiplex PCR-based targeted enrichment scheme 6

7 GeneRead DNAseq Targeted panels (V2) Type Product name No. of total primer pairs Size (kb) No. of Target Gene Specifi city Uniformity (0.2x mean) Experim ental coverage Solid Tumor Hematologic malignancies Clinically Relevant Tumor Panel % 90% 90% Tumor Actionable Mutations Panel % 91% 91% Human Myeloid Neoplasms Panel % 94% 94% Human Breast Cancer Panel % 91% 91% Human Colorectal Cancer Panel % 95% 95% Human Liver Cancer Panel % 96% 96% Tissue Specific Human Lung Cancer Panel % 90% 90% Human Ovarian Cancer Panel % 96% 96% Human Prostate Cancer Panel % 94% 94% Human Gastric Cancer Panel % 93% 93% Human Cardiomyopathy Panel % 87% 87% Comprehensive Human Cancer Predisposition Panel Human Comprehensive Cancer Panel % 93% 93% % 92% 92% Human Carrier Testing Panel % 91% 91% 7

8 QIAseq Targeted DNAseq panels (V3) Digital DNA sequencing to confidently detect low-frequency variants: Digital sequencing enabled by molecular barcodes to remove PCR duplicates Complete solution streamlines the workflow Compatibility with low-quality DNA enables efficient sequencing of FFPE and cfdna samples Minimal DNA input to preserve precious samples (20 ng input DNA) Optimized buffers and conditions to achieve high coverage of GC-rich regions Applications: SNVs Small indels CNVs Workflow 8

9 QIAseq Targeted DNAseq panels (V3) Molecular barcode is a tag (barcode) to identify unique DNA or RNA molecules Principle of molecular barcodes 9

10 QIAseq Targeted DNAseq panels (V3) Catalog no. Product name No. of genes No. of total primers* DHS-001Z Human Breast Cancer Panel DHS-002Z Human Colorectal Cancer Panel DHS-003Z Human Myeloid Neoplasms Panel DHS-005Z Human Lung Cancer Panel DHS-104Z Human Pharmacogenomics Panel DHS-3011Z Human Inherited Disease Panel DHS-3501Z Human Comprehensive Cancer Panel DHS-101Z Human Actionable Solid Tumor Panel DHS-102Z Human BRCA1 and BRCA2 Panel DHS-103Z Human BRCA1 and BRCA2 Plus Panel DHS-105Z Human Mitochondria Panel Chromosome M Type of Coverage * * Types of coverage: 1. Exonic regions of genes plus 10 bases to cover intron/exon junctions 2. Mix of type of coverage 1 (for tumor suppressor genes) and HotSpots for Oncogenes 3. SNPs 4. Full chromosome 10

11 QIAGEN s solution for RNA-seq QIAseq Targeted RNA Panels and QIAseq targeted RNAscan panels have been developed as a solution for quantitative gene expression and gene fusion profiling using RNA-seq. 11

12 QIAseq Targeted RNA Panels and RNAscan Panels Panels QIAseq Targeted RNA Panels QIAseq Targeted RNAscan Panels Descriptions Digital RNAseq for gene expression profiling Ability to examine hundreds of genes in hundreds of samples simultaneously Requires only 25 ng of total RNA Go from sample to library in one day Accurate and digital gene expression profiling using molecular barcodes Applying digital RNA sequencing to scan for known and novel fusion genes Accurate quantification of a large number of fusion genes Identify new fusion gene partners, no prior knowledge of breakpoint needed Works with low-quality RNA, including RNA from FFPE and liquid biopsy Requires low RNA input, as low as 15 ng of un-enriched RNA Automation-friendly Title, Location, Date 12

13 QIAseq Targeted RNA Panels Applications: Gene expression profiling Biomarker research Confirmation of whole transcriptome sequencing data Confirmation of microarray data 13

14 QIAseq Targeted RNA Panels Digital sequencing(molecular barcodes) principle Different platforms comparison 14

15 Current QIAseq Targeted RNA Panels (Human) Product name No. of genes Human Angiogenesis & Endothelial Cell Biology 340 Human Apoptosis & Cell Death 264 Human Cancer Transcriptome 395 QIAseq Targeted RNA Panels Human Extracellular Matrix & Cell Adhesion Molecules 421 Human Inflammation & Immunity Transcriptome 475 Human Molecular Toxicology Transcriptome 370 Human Signal Transduction PathwayFinder 405 Human Stem Cell & Differentiation Markers 293 QIAseq Targeted RNA Extended Panels QIAseq Targeted RNA Custom Panel QIAseq Targeted RNA Extended Panel QIAseq Targeted RNA Custom Panels Customized Customized 15

16 QIAseq Targeted RNAscan Panels Applications: Detection of known gene fusions based on well-characterized breakpoints Discovery of novel gene fusions using exon- or gene-based primer designs Workflow 16

17 QIAseq Targeted RNAscan Panels Technical advantage Principle of molecular barcodes The unique combination of our proprietary primer design algorithm and rigorous testing of every primer assay guarantees high specificity and accurate results 17

18 Current QIAseq Targeted RNAscan Panels Panel Product Name No.total primers No.total gene Human Leukemia Panel QIAseq Targeted RNAscan Panels Human Solid Tumor Panel Human Lung Cancer Panel Human Oncology Panel Custom Panel QIAseq Targeted RNAscan Custom Panels Customized Customized 18

19 QIAGEN s solution for Single Cell Sequencing QIAGEN can provide solution for single cell sequencing from single-cell isolation to data interpretation 19

20 Single Cell Genomics by QIAGEN QIAscout For accessible, affordable and efficient isolation and recovery of single cells. REPLI-g Cell WGA & WTA Kit For parallel whole genome and whole transcriptome amplification from cells and limited samples. REPLI-g Single Cell Kit For highly uniform whole genome amplification (WGA) from single cells or limited sample material. REPLI-g WTA Single Cell Kit For whole transcriptome amplification of total RNA or mrna from single cells. REPLI-g Single Cell DNA Library Kit For DNA library construction from single cells for Illumina sequencing applications. REPLI-g Single Cell RNA Library Kit For RNA library construction from single cells for Illumina sequencing applications. 20

21 The QIAseq FX Single Cell DNA/RNA Library Kit QIAseq FX Single Cell DNA Library Kit QIAseq FX Single Cell RNA Library Kit Single-cell whole genome libraries with comprehensive coverage and high sequence fidelity. The kit provides a complete solution for whole genome sequencing from isolated single animal or bacterial cells or low amounts of genomic DNA. The kit includes all reagents required for cell lysis, whole genome amplification, enzymatic DNA fragmentation and PCR-free NGS library preparation. Single cell RNA-seq libraries that provide a deeper view of the transcriptome. The QIAseq FX Single Cell RNA Library kit is an end-to-end library preparation solution for RNAseq from single cells or low amounts of RNA. The kit includes all reagents required for cell lysis, reverse transcription, cdna amplification and PCR-free NGS library preparation. 21

22 The Multiple Displacement Amplification (MDA) technology REPLI-g and QIAseq FX uses isothermal genome amplification, termed MDA, which involves the binding of random hexamers to denatured DNA followed by strand displacement synthesis at a constant temperature using the enzyme Phi 29 polymerase. REPLI-g MDA technology delivers long read lengths with isothermal amplification Unbiased amplification with Phi29 polymerase 22

23 Our powerful Data Analysis and Visualization-Bioinformatics From sample to insight with QIAGEN Bioinformatics End-to-end NGS data analysis solution and from data to biological insights; With our workflows, it enables easy and accurate discovery, verification, and validation of novel disease in cancer and hereditary disease NGS data. 23

24 Tumor Mutation Burden (TMB) - Assay Flowchart (1) 24

25 Tumor Mutation Burden (TMB) - Assay Flowchart (2) 25

26 Product development based on NGS-TMB detection 26

27 Our IP of NGS Computer software copyright registration certificate PubMed mass document format conversion system based on MEDLINE format; High - throughput sequencing data analysis software; Data analysis software based on VCF format for gene variation; RNA-seq analysis software based on PDX model; Gene expression profiling data mining analysis software; 27

28 Contact US Nick Zhang, Ph.D. CEO Address: Suite 901, B5 Building, 218 Xinghu St. Suzhou Industrial Park, China ZIP: Business Contact Wei Zhang (Wesley), Ph.D., Director of Business Development Phone: Mobile: Technical Contact Congmao Wang, Ph.D., Manager of NGS & Bioinformatics 28

Sample to Insight. Dr. Bhagyashree S. Birla NGS Field Application Scientist

Sample to Insight. Dr. Bhagyashree S. Birla NGS Field Application Scientist Dr. Bhagyashree S. Birla NGS Field Application Scientist bhagyashree.birla@qiagen.com NGS spans a broad range of applications DNA Applications Human ID Liquid biopsy Biomarker discovery Inherited and somatic

More information

Welcome to the NGS webinar series

Welcome to the NGS webinar series Welcome to the NGS webinar series Webinar 1 NGS: Introduction to technology, and applications NGS Technology Webinar 2 Targeted NGS for Cancer Research NGS in cancer Webinar 3 NGS: Data analysis for genetic

More information

NGS: Digital RNAseq & Library Prep Seminar. Next-Generation Sequencing Lunch & Learn

NGS: Digital RNAseq & Library Prep Seminar. Next-Generation Sequencing Lunch & Learn NGS: Digital RNAseq & Library Prep Seminar Next-Generation Sequencing Lunch & Learn Samuel Rulli, Ph. D Global Product Manager QIAseq Targeted RNA Panels 1 Targeted sequencing with UMIs QIAseq mirnaseq

More information

Accelerate your NGS performance through Sample to Insight solutions

Accelerate your NGS performance through Sample to Insight solutions Accelerate your NGS performance through Sample to Insight solutions Complete your NGS workflow and save up to 40% Sample to Insight From Sample to Insight let QIAGEN enhance your NGS-based research High-throughput

More information

QIAseq SPE technology for Illumina : Redefining amplicon sequencing

QIAseq SPE technology for Illumina : Redefining amplicon sequencing Application Note QIAseq SPE technology for Illumina : Redefining amplicon sequencing Amplicon-based enrichment and sequencing takes advantage of PCR workflows to turn amplicons that represent regions of

More information

Development of quantitative targeted RNA-seq methodology for use in differential gene expression

Development of quantitative targeted RNA-seq methodology for use in differential gene expression Development of quantitative targeted RNA-seq methodology for use in differential gene expression Dr. Jens Winter, Market Development Group Biological Biological Research Content EMEA QIAGEN Universal Workflows

More information

Introducing QIAseq. Accelerate your NGS performance through Sample to Insight solutions. Sample to Insight

Introducing QIAseq. Accelerate your NGS performance through Sample to Insight solutions. Sample to Insight Introducing QIAseq Accelerate your NGS performance through Sample to Insight solutions Sample to Insight From Sample to Insight let QIAGEN enhance your NGS-based research High-throughput next-generation

More information

Exploring new frontiers with next-generation sequencing

Exploring new frontiers with next-generation sequencing Spotlight: NGS Exploring new frontiers with next-generation sequencing Pushing the limits of discovery Next-generation sequencing (NGS) is being utilized for numerous new and exciting applications, such

More information

Accessible answers. Targeted sequencing: accelerating and amplifying answers for oncology research

Accessible answers. Targeted sequencing: accelerating and amplifying answers for oncology research Accessible answers Targeted sequencing: accelerating and amplifying answers for oncology research Help advance precision medicine Accelerate results with Ion Torrent NGS Life without cancer. This is our

More information

Next Generation Sequencing. Target Enrichment

Next Generation Sequencing. Target Enrichment Next Generation Sequencing Target Enrichment Next Generation Sequencing Your Partner in Every Step from Sample to Data NGS: Revolutionizing Genetic Analysis with Single-Molecule Resolution Next generation

More information

Your Best Data: Teaming QIAGEN Chemistry & Bioinformatics to Drive Samples to Insight

Your Best Data: Teaming QIAGEN Chemistry & Bioinformatics to Drive Samples to Insight Your Best Data: Teaming QIAGEN Chemistry & Bioinformatics to Drive Samples to Insight Aysel Heckel Director Clinical Solutions Sales Dr. Anne Arens Field Application Scientist Course on Variant Detection

More information

Digital DNA/RNA sequencing enables highly accurate and sensitive biomarker detection and quantification

Digital DNA/RNA sequencing enables highly accurate and sensitive biomarker detection and quantification Digital DNA/RNA sequencing enables highly accurate and sensitive biomarker detection and quantification Erwin Chen ( 陳立德 ) Technical Product Specialist QIAGEN Taiwan Precision medicine: Right drug, right

More information

Get to Know Your DNA. Every Single Fragment.

Get to Know Your DNA. Every Single Fragment. HaloPlex HS NGS Target Enrichment System Get to Know Your DNA. Every Single Fragment. High sensitivity detection of rare variants using molecular barcodes How Does Molecular Barcoding Work? HaloPlex HS

More information

DNA. Clinical Trials. Research RNA. Custom. Reports CLIA CAP GCP. Tumor Genomic Profiling Services for Clinical Trials

DNA. Clinical Trials. Research RNA. Custom. Reports CLIA CAP GCP. Tumor Genomic Profiling Services for Clinical Trials Tumor Genomic Profiling Services for Clinical Trials Custom Reports DNA RNA Focused Gene Sets Clinical Trials Accuracy and Content Enhanced NGS Sequencing Extended Panel, Exomes, Transcriptomes Research

More information

Ion S5 and Ion S5 XL Systems

Ion S5 and Ion S5 XL Systems Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5

More information

Surely Better Target Enrichment from Sample to Sequencer

Surely Better Target Enrichment from Sample to Sequencer sureselect TARGET ENRICHMENT solutions Surely Better Target Enrichment from Sample to Sequencer Agilent s market leading SureSelect platform provides a complete portfolio of catalog to custom products,

More information

QIAGEN s Preanalytic NGS Solutions

QIAGEN s Preanalytic NGS Solutions QIAGEN s Preanalytic NGS Solutions For outstanding DNAseq results Choose QIAGEN for every step of your NGS workflow Sample & Assay Technologies 1. Next-generation sequencing (NGS) Purification and Sample

More information

Ion S5 and Ion S5 XL Systems

Ion S5 and Ion S5 XL Systems Ion S5 and Ion S5 XL Systems Targeted sequencing has never been simpler Explore the Ion S5 and Ion S5 XL Systems Adopting next-generation sequencing (NGS) in your lab is now simpler than ever The Ion S5

More information

SureSelect XT HS. Target Enrichment

SureSelect XT HS. Target Enrichment SureSelect XT HS Target Enrichment What Is It? SureSelect XT HS joins the SureSelect library preparation reagent family as Agilent s highest sensitivity hybrid capture-based library prep and target enrichment

More information

HaloPlex HS. Get to Know Your DNA. Every Single Fragment. Kevin Poon, Ph.D.

HaloPlex HS. Get to Know Your DNA. Every Single Fragment. Kevin Poon, Ph.D. HaloPlex HS Get to Know Your DNA. Every Single Fragment. Kevin Poon, Ph.D. Sr. Global Product Manager Diagnostics & Genomics Group Agilent Technologies For Research Use Only. Not for Use in Diagnostic

More information

CAPTURE-BASED APPROACH FOR COMPREHENSIVE DETECTION OF IMPORTANT ALTERATIONS

CAPTURE-BASED APPROACH FOR COMPREHENSIVE DETECTION OF IMPORTANT ALTERATIONS CAPTURE-BASE APPROACH FOR COMPREHENSIVE ETECTION OF IMPORTANT ALTERATIONS SEQUENCE MUTATIONS MICROSATELLITE INSTABILITY AMPLIFICATIONS GENOMIC REARRANGEMENTS For Research Use Only. Not for iagnostic Purposes.

More information

Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES

Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES Roche Sequencing Solutions CHANGING SCIENCE CHANGING LIVES Roche Sequencing Solutions Roche is helping to shape the future of personalized medicine by integrating best-in-class sequencing technologies

More information

Agilent NGS Solutions : Addressing Today s Challenges

Agilent NGS Solutions : Addressing Today s Challenges Agilent NGS Solutions : Addressing Today s Challenges Charmian Cher, Ph.D Director, Global Marketing Programs 1 10 years of Next-Gen Sequencing 2003 Completion of the Human Genome Project 2004 Pyrosequencing

More information

Enterprise Interest I am an employee of ThermoFisher Scientific.

Enterprise Interest I am an employee of ThermoFisher Scientific. Enterprise Interest I am an employee of ThermoFisher Scientific. Developing a multiplex next-generation sequencing assay to study highly clonal tumor samples Dumitru Brinza, Ph.D Clinical Next-Generation

More information

MULTIPLEXING SIMULTANEOUSLY DETECT MULTIPLE TARGETS IN SINGLE ASSAYS. WhiteSci Whitehead Scientific (Pty) Ltd. Products. Expertise. Support.

MULTIPLEXING SIMULTANEOUSLY DETECT MULTIPLE TARGETS IN SINGLE ASSAYS. WhiteSci Whitehead Scientific (Pty) Ltd. Products. Expertise. Support. MULTIPLEXING SIMULTANEOUSLY DETECT MULTIPLE TARGETS IN SINGLE ASSAYS WhiteSci Whitehead Scientific (Pty) Ltd Products. Expertise. Support. MULTIPLEXING Allowing researchers to gain more insight into precious

More information

resequencing storage SNP ncrna metagenomics private trio de novo exome ncrna RNA DNA bioinformatics RNA-seq comparative genomics

resequencing storage SNP ncrna metagenomics private trio de novo exome ncrna RNA DNA bioinformatics RNA-seq comparative genomics RNA Sequencing T TM variation genetics validation SNP ncrna metagenomics private trio de novo exome mendelian ChIP-seq RNA DNA bioinformatics custom target high-throughput resequencing storage ncrna comparative

More information

Integrated NGS Sample Preparation Solutions for Limiting Amounts of RNA and DNA. March 2, Steven R. Kain, Ph.D. ABRF 2013

Integrated NGS Sample Preparation Solutions for Limiting Amounts of RNA and DNA. March 2, Steven R. Kain, Ph.D. ABRF 2013 Integrated NGS Sample Preparation Solutions for Limiting Amounts of RNA and DNA March 2, 2013 Steven R. Kain, Ph.D. ABRF 2013 NuGEN s Core Technologies Selective Sequence Priming Nucleic Acid Amplification

More information

Titelstijl van model bewerken

Titelstijl van model bewerken Generate Titelstijl van and verify model your bewerken data Solutions for all your genetic analysis needs Sanger Sequencing Microarray technology QuantStudio real-time and digital PCR Ion Torrent NGS systems

More information

A Genomics (R)evolution: Harnessing the Power of Single Cells

A Genomics (R)evolution: Harnessing the Power of Single Cells A Genomics (R)evolution: Harnessing the Power of Single Cells Fundamental Question #1 If Transcriptional Heterogeneity ( Noise ) is so great in single cells What s the Point? Single Cells = True Biology

More information

About Strand NGS. Strand Genomics, Inc All rights reserved.

About Strand NGS. Strand Genomics, Inc All rights reserved. About Strand NGS Strand NGS-formerly known as Avadis NGS, is an integrated platform that provides analysis, management and visualization tools for next-generation sequencing data. It supports extensive

More information

G E N OM I C S S E RV I C ES

G E N OM I C S S E RV I C ES GENOMICS SERVICES ABOUT T H E N E W YOR K G E NOM E C E N T E R NYGC is an independent non-profit implementing advanced genomic research to improve diagnosis and treatment of serious diseases. Through

More information

Introduction into single-cell RNA-seq. Kersti Jääger 19/02/2014

Introduction into single-cell RNA-seq. Kersti Jääger 19/02/2014 Introduction into single-cell RNA-seq Kersti Jääger 19/02/2014 Cell is the smallest functional unit of life Nucleus.ATGC.UACG. A Cell KLTSH. The complexity of biology How many cell types? How many cells?

More information

Isolation of total nucleic acids from FFPE tissues using FormaPure DNA

Isolation of total nucleic acids from FFPE tissues using FormaPure DNA APPLICATION NOTE Isolation of total nucleic acids from FFPE tissues using FormaPure DNA Jung Hoon Doh, Ph.D. Senior Application Scientist Beckman Coulter Life Sciences, Indianapolis, IN USA Summary Extensive

More information

Access Array BRCA1 / BRCA2 / TP53 Target-Specific Panel Build the highest quality amplicon libraries with qualified assays

Access Array BRCA1 / BRCA2 / TP53 Target-Specific Panel Build the highest quality amplicon libraries with qualified assays DATA SHEET PN 100-3489 B1 Access Array BRCA1 / BRCA2 / TP53 Target-Specific Panel Build the highest quality amplicon libraries with qualified assays Covers 100% of the exons within the genes Supported

More information

Increased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit

Increased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit be INSPIRED drive DISCOVERY stay GENUINE TECHNICAL NOTE Increased transcription detection with the NEBNext Single Cell/Low Input RNA Library Prep Kit Highly sensitive, robust generation of high quality

More information

Cancer Genetics Solutions

Cancer Genetics Solutions Cancer Genetics Solutions Cancer Genetics Solutions Pushing the Boundaries in Cancer Genetics Cancer is a formidable foe that presents significant challenges. The complexity of this disease can be daunting

More information

Introduction to RNA-Seq in GeneSpring NGS Software

Introduction to RNA-Seq in GeneSpring NGS Software Introduction to RNA-Seq in GeneSpring NGS Software Dipa Roy Choudhury, Ph.D. Strand Scientific Intelligence and Agilent Technologies Learn more at www.genespring.com Introduction to RNA-Seq In a few years,

More information

Product selection guide Ion GeneStudio S5 Series

Product selection guide Ion GeneStudio S5 Series Cancer genomics research Molecular profiling Ion AmpliSeq Comprehensive Cancer Panel Cat. No. 4477685 Ion AmpliSeq Made-to-Order Panels (Customize your own or browse redesigned community panels at ampliseq.com)

More information

TREE CODE PRODUCT BROCHURE

TREE CODE PRODUCT BROCHURE TREE CODE PRODUCT BROCHURE Single Molecule, Real-Time (SMRT) Sequencing technology offers: Long read sequencing ~10 Gb with 20 kb average read lengths for WGS ~20 Gb with 40 kb average read length for

More information

The Expanded Illumina Sequencing Portfolio New Sample Prep Solutions and Workflow

The Expanded Illumina Sequencing Portfolio New Sample Prep Solutions and Workflow The Expanded Illumina Sequencing Portfolio New Sample Prep Solutions and Workflow Marcus Hausch, Ph.D. 2010 Illumina, Inc. All rights reserved. Illumina, illuminadx, Solexa, Making Sense Out of Life, Oligator,

More information

QPCR ASSAYS FOR MIRNA EXPRESSION PROFILING

QPCR ASSAYS FOR MIRNA EXPRESSION PROFILING TECH NOTE 4320 Forest Park Ave Suite 303 Saint Louis, MO 63108 +1 (314) 833-9764 mirna qpcr ASSAYS - powered by NAWGEN Our mirna qpcr Assays were developed by mirna experts at Nawgen to improve upon previously

More information

MICROARRAYS+SEQUENCING

MICROARRAYS+SEQUENCING MICROARRAYS+SEQUENCING The most efficient way to advance genomics research Down to a Science. www.affymetrix.com/downtoascience Affymetrix GeneChip Expression Technology Complementing your Next-Generation

More information

The MiniSeq System. Explore the possibilities. Discover demonstrated NGS workflows for molecular biology applications.

The MiniSeq System. Explore the possibilities. Discover demonstrated NGS workflows for molecular biology applications. The MiniSeq System. Explore the possibilities. Discover demonstrated NGS workflows for molecular biology applications. Let your work flow with Illumina NGS. The MiniSeq System delivers powerful and cost-effective

More information

Almac Diagnostics. NGS Panels: From Patient Selection to CDx. Dr Katarina Wikstrom Head of US Operations Almac Diagnostics

Almac Diagnostics. NGS Panels: From Patient Selection to CDx. Dr Katarina Wikstrom Head of US Operations Almac Diagnostics Almac Diagnostics NGS Panels: From Patient Selection to CDx Dr Katarina Wikstrom Head of US Operations Almac Diagnostics Overview Almac Diagnostics Overview Benefits and Challenges of NGS Panels for Subject

More information

Simultaneous genome and transcriptome sequencing

Simultaneous genome and transcriptome sequencing Simultaneous genome and transcriptome sequencing Technical Journal Club 31.01.2017 Christina Müller Introduction Rapid growth of single cell genomics www.biomarkerinsights.qiagen.com Introduction Single

More information

Molecular analysis via next-generation sequencing

Molecular analysis via next-generation sequencing Molecular analysis via next-generation sequencing NMG Annual Fall Meeting, Toronto, Canada Raed Samara, PhD, PMP Global Product Manager October 21, 2013 Legal Disclaimer QIAGEN products shown here are

More information

Surely Better Target Enrichment from Sample to Sequencer and Analysis

Surely Better Target Enrichment from Sample to Sequencer and Analysis sureselect TARGET ENRIChment solutions Surely Better Target Enrichment from Sample to Sequencer and Analysis Agilent s market leading SureSelect platform provides a complete portfolio of catalog to custom

More information

Next Generation Oncology Sequencing in your Laboratory. Built by pioneers in cancer genomics and liquid biopsy approaches PROGENEUS

Next Generation Oncology Sequencing in your Laboratory. Built by pioneers in cancer genomics and liquid biopsy approaches PROGENEUS PROGENEUS Next Generation Oncology Sequencing in your Laboratory Built by pioneers in cancer genomics and liquid biopsy approaches For Research Use Only. Not for iagnostic Purposes. personalgenome.com/progeneus

More information

FFPE in your NGS Study

FFPE in your NGS Study FFPE in your NGS Study Richard Corbett Canada s Michael Smith Genome Sciences Centre Vancouver, British Columbia Dec 6, 2017 Our mandate is to advance knowledge about cancer and other diseases and to use

More information

Novel methods for RNA and DNA- Seq analysis using SMART Technology. Andrew Farmer, D. Phil. Vice President, R&D Clontech Laboratories, Inc.

Novel methods for RNA and DNA- Seq analysis using SMART Technology. Andrew Farmer, D. Phil. Vice President, R&D Clontech Laboratories, Inc. Novel methods for RNA and DNA- Seq analysis using SMART Technology Andrew Farmer, D. Phil. Vice President, R&D Clontech Laboratories, Inc. Agenda Enabling Single Cell RNA-Seq using SMART Technology SMART

More information

SureSelect Target Enrichment for the Ion Proton TM Next Generation Sequencing System

SureSelect Target Enrichment for the Ion Proton TM Next Generation Sequencing System SureSelect Target Enrichment for the Ion Proton TM Next Generation Sequencing System Demonstrated performance you can count on Christina Chiu Product Manager, SureSelect Kyeong Jeong Ph.D. R&D Scientist

More information

Incorporating Molecular ID Technology. Accel-NGS 2S MID Indexing Kits

Incorporating Molecular ID Technology. Accel-NGS 2S MID Indexing Kits Incorporating Molecular ID Technology Accel-NGS 2S MID Indexing Kits Molecular Identifiers (MIDs) MIDs are indices used to label unique library molecules MIDs can assess duplicate molecules in sequencing

More information

RIPTIDE HIGH THROUGHPUT RAPID LIBRARY PREP (HT-RLP)

RIPTIDE HIGH THROUGHPUT RAPID LIBRARY PREP (HT-RLP) Application Note: RIPTIDE HIGH THROUGHPUT RAPID LIBRARY PREP (HT-RLP) Introduction: Innovations in DNA sequencing during the 21st century have revolutionized our ability to obtain nucleotide information

More information

Supplementary Information for:

Supplementary Information for: Supplementary Information for: A streamlined and high-throughput targeting approach for human germline and cancer genomes using Oligonucleotide-Selective Sequencing Samuel Myllykangas 1, Jason D. Buenrostro

More information

Automation of Lexogen s QuantSeq 3 mrna-seq Library Prep Kits on the Biomek FX p NGS Workstation

Automation of Lexogen s QuantSeq 3 mrna-seq Library Prep Kits on the Biomek FX p NGS Workstation Automation of Lexogen s QuantSeq 3 mrna-seq Library Prep Kits on the Biomek FX p NGS Workstation The Lexogen QuantSeq 3 mrna-seq Library Prep Kits for Illumina (FWD and REV) produce ready-to-sequence libraries

More information

Functional DNA Quality Analysis Improves the Accuracy of Next Generation Sequencing from Clinical Specimens

Functional DNA Quality Analysis Improves the Accuracy of Next Generation Sequencing from Clinical Specimens Functional DNA Quality Analysis Improves the Accuracy of Next Generation Sequencing from Clinical Specimens Overview We have developed a novel QC, the SuraSeq DNA Quantitative Functional Index (QFI ).

More information

Gene Regulation Solutions. Microarrays and Next-Generation Sequencing

Gene Regulation Solutions. Microarrays and Next-Generation Sequencing Gene Regulation Solutions Microarrays and Next-Generation Sequencing Gene Regulation Solutions The Microarrays Advantage Microarrays Lead the Industry in: Comprehensive Content SurePrint G3 Human Gene

More information

SMARTer Ultra Low RNA Kit for Illumina Sequencing Two powerful technologies combine to enable sequencing with ultra-low levels of RNA

SMARTer Ultra Low RNA Kit for Illumina Sequencing Two powerful technologies combine to enable sequencing with ultra-low levels of RNA SMARTer Ultra Low RNA Kit for Illumina Sequencing Two powerful technologies combine to enable sequencing with ultra-low levels of RNA The most sensitive cdna synthesis technology, combined with next-generation

More information

Product selection guide Ion S5 and Ion S5 XL Systems

Product selection guide Ion S5 and Ion S5 XL Systems Product selection guide Ion S5 and s Cancer genomics research Molecular profiling Ion AmpliSeq Ready-to-Use Panels Ion AmpliSeq Comprehensive Cancer Panel Cat. No. 4477685 Ion AmpliSeq Made-to-Order Panels

More information

Developing an Accurate and Precise Companion Diagnostic Assay for Targeted Therapies in DLBCL

Developing an Accurate and Precise Companion Diagnostic Assay for Targeted Therapies in DLBCL Developing an Accurate and Precise Companion Diagnostic Assay for Targeted Therapies in DLBCL James Storhoff, Ph.D. Senior Manager, Diagnostic Test Development World Cdx, Boston, Sep. 10th Molecules That

More information

Target Enrichment Strategies for Next Generation Sequencing

Target Enrichment Strategies for Next Generation Sequencing Target Enrichment Strategies for Next Generation Sequencing Anuj Gupta, PhD Agilent Technologies, New Delhi Genotypic Conference, Sept 2014 NGS Timeline Information burst Nearly 30,000 human genomes sequenced

More information

Gene Expression Analysis with Pathway-Centric DNA Microarrays

Gene Expression Analysis with Pathway-Centric DNA Microarrays Gene Expression Analysis with Pathway-Centric DNA Microarrays SuperArray Bioscience Corporation George J. Quellhorst, Jr. Ph.D. Manager, Customer Education Topics to be Covered Introduction to DNA Microarrays

More information

Bioinformatics Advice on Experimental Design

Bioinformatics Advice on Experimental Design Bioinformatics Advice on Experimental Design Where do I start? Please refer to the following guide to better plan your experiments for good statistical analysis, best suited for your research needs. Statistics

More information

APPLICATION NOTE. Abstract. Introduction

APPLICATION NOTE. Abstract. Introduction From minuscule amounts to magnificent results: reliable ChIP-seq data from 1, cells with the True MicroChIP and the MicroPlex Library Preparation kits Abstract Diagenode has developed groundbreaking solutions

More information

Oncomine cfdna Assays Part III: Variant Analysis

Oncomine cfdna Assays Part III: Variant Analysis Oncomine cfdna Assays Part III: Variant Analysis USER GUIDE for use with: Oncomine Lung cfdna Assay Oncomine Colon cfdna Assay Oncomine Breast cfdna Assay Catalog Numbers A31149, A31182, A31183 Publication

More information

Personalized CAR-T Immunotherapy Platform

Personalized CAR-T Immunotherapy Platform GLP, GMP, and CLIA-Certified Lab Personalized CAR-T Immunotherapy Platform Accelerate your cancer research and drug discovery Platform Overview 1500 Existing Hybridomas and Antibody Engineering Custom

More information

lncrna Gene Expression Simplified Long Noncoding RNA Discovery

lncrna Gene Expression Simplified Long Noncoding RNA Discovery lncrna Gene Expression Simplified Long Noncoding RNA Discovery Innovative Tools to Simplify lncrna Discovery Begin exploring the long noncoding transcriptome with sensitive and simple-to-use lncrna assays

More information

Gene expression microarrays and assays. Because your results can t wait

Gene expression microarrays and assays. Because your results can t wait Gene expression microarrays and assays Because your results can t wait A simple path from data to decision-making The power of expression microarrays Transcriptome-wide analysis can be complex. Matching

More information

SURESELECTXT LOW INPUT TARGET ENRICHMENT

SURESELECTXT LOW INPUT TARGET ENRICHMENT SURESELECTXT LOW INPUT TARGET ENRICHMENT Low Input FFPE Optimized Streamlined Workflow SureSelect XT Low Input What is it? SureSelect XT Low Input is a low-input, FFPE-optimized library preparation kit.

More information

Next Generation Sequencing: Data analysis for genetic profiling

Next Generation Sequencing: Data analysis for genetic profiling Next Generation Sequencing: Data analysis for genetic profiling Raed Samara, Ph.D. Global Product Manager Raed.Samara@QIAGEN.com Welcome to the NGS webinar series - 2015 NGS Technology Webinar 1 NGS: Introduction

More information

to precision medicine

to precision medicine QIAGEN at the AMP 2018 Annual Meeting yourpath Discover to precision medicine Sample to Insight Discover your path to precision medicine Lead the way with QIAGEN, from Sample to Insight Every day, data

More information

The Agilent Technologies SureSelect Platform for Target Enrichment

The Agilent Technologies SureSelect Platform for Target Enrichment The Agilent Technologies SureSelect Platform for Target Enrichment Focus your next-gen sequencing on DNA that matters Kimberly Troutman Field Applications Scientist January 27 th, 2011 Agenda 1 Introduction:

More information

Lab methods: Exome / Genome. Ewart de Bruijn

Lab methods: Exome / Genome. Ewart de Bruijn Lab methods: Exome / Genome 27 06 2013 Ewart de Bruijn Library prep is only a small part of the complete DNA analysis workflow DNA isolation library prep enrichment flowchip prep sequencing bioinformatics

More information

High Cross-Platform Genotyping Concordance of Axiom High-Density Microarrays and Eureka Low-Density Targeted NGS Assays

High Cross-Platform Genotyping Concordance of Axiom High-Density Microarrays and Eureka Low-Density Targeted NGS Assays High Cross-Platform Genotyping Concordance of Axiom High-Density Microarrays and Eureka Low-Density Targeted NGS Assays Ali Pirani and Mohini A Patil ISAG July 2017 The world leader in serving science

More information

Human Genome Sequencing Over the Decades The capacity to sequence all 3.2 billion bases of the human genome (at 30X coverage) has increased

Human Genome Sequencing Over the Decades The capacity to sequence all 3.2 billion bases of the human genome (at 30X coverage) has increased Human Genome Sequencing Over the Decades The capacity to sequence all 3.2 billion bases of the human genome (at 30X coverage) has increased exponentially since the 1990s. In 2005, with the introduction

More information

High Throughput Sequencing the Multi-Tool of Life Sciences. Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center

High Throughput Sequencing the Multi-Tool of Life Sciences. Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center High Throughput Sequencing the Multi-Tool of Life Sciences Lutz Froenicke DNA Technologies and Expression Analysis Cores UCD Genome Center Complementary Approaches Illumina Still-imaging of clusters (~1000

More information

Non-Organic-Based Isolation of Mammalian microrna using Norgen s microrna Purification Kit

Non-Organic-Based Isolation of Mammalian microrna using Norgen s microrna Purification Kit Application Note 13 RNA Sample Preparation Non-Organic-Based Isolation of Mammalian microrna using Norgen s microrna Purification Kit B. Lam, PhD 1, P. Roberts, MSc 1 Y. Haj-Ahmad, M.Sc., Ph.D 1,2 1 Norgen

More information

MassARRAY Genetic Analysis System. Genotyping Methylation Analysis Molecular Typing Somatic Mutation Profiling Quantitative Gene Expression (QGE)

MassARRAY Genetic Analysis System. Genotyping Methylation Analysis Molecular Typing Somatic Mutation Profiling Quantitative Gene Expression (QGE) MassARRAY Genetic Analysis System Genotyping Methylation Analysis Molecular Typing Somatic Mutation Profiling Quantitative Gene Expression (QGE) MassARRAY Genetic Analysis System * Overview Next-generation

More information

Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina be INSPIRED drive DISCOVERY stay GENINE TECHNICAL NOTE Directional rrna depletion Obtain superior NGS library performance with lower input amounts using the NEBNext ltra II Directional RNA Library Prep

More information

Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina

Obtain superior NGS library performance with lower input amounts using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina be INSPIRED drive DISCOVERY stay GENINE TECHNICAL NOTE Directional rrna depletion Obtain superior NGS library performance with lower input amounts using the NEBNext ltra II Directional RNA Library Prep

More information

Basics of RNA-Seq. (With a Focus on Application to Single Cell RNA-Seq) Michael Kelly, PhD Team Lead, NCI Single Cell Analysis Facility

Basics of RNA-Seq. (With a Focus on Application to Single Cell RNA-Seq) Michael Kelly, PhD Team Lead, NCI Single Cell Analysis Facility 2018 ABRF Meeting Satellite Workshop 4 Bridging the Gap: Isolation to Translation (Single Cell RNA-Seq) Sunday, April 22 Basics of RNA-Seq (With a Focus on Application to Single Cell RNA-Seq) Michael Kelly,

More information

DNA concentration and purity were initially measured by NanoDrop 2000 and verified on Qubit 2.0 Fluorometer.

DNA concentration and purity were initially measured by NanoDrop 2000 and verified on Qubit 2.0 Fluorometer. DNA Preparation and QC Extraction DNA was extracted from whole blood or flash frozen post-mortem tissue using a DNA mini kit (QIAmp #51104 and QIAmp#51404, respectively) following the manufacturer s recommendations.

More information

Performance Characteristics drmid Dx for Illumina NGS systems

Performance Characteristics drmid Dx for Illumina NGS systems Performance Characteristics drmid Dx for Illumina NGS systems MANUFACTURER Multiplicom N.V. Galileïlaan 18 2845 Niel BELGIUM Revision date: August, 2017 Page 1 of 7 TABLE OF CONTENTS 1. TEST PRINCIPLE...

More information

Transcriptomics analysis with RNA seq: an overview Frederik Coppens

Transcriptomics analysis with RNA seq: an overview Frederik Coppens Transcriptomics analysis with RNA seq: an overview Frederik Coppens Platforms Applications Analysis Quantification RNA content Platforms Platforms Short (few hundred bases) Long reads (multiple kilobases)

More information

Germline Genotyping and Highly Sensitive Mutation Detection on the MassARRAY System

Germline Genotyping and Highly Sensitive Mutation Detection on the MassARRAY System Sensitivity Across the Spectrum Results Reporting iplex and UltraSEEK chemistries are compatible with a broad range of nucleic acid biomarkers, from standard germline genotypes to rare somatic variants.

More information

Illumina Genome Analyzer. Progenika Experience. - Susana Catarino -

Illumina Genome Analyzer. Progenika Experience. - Susana Catarino - Illumina Genome Analyzer Progenika Experience - Susana Catarino - Who are we? 2000 PROGENIKA BIOPHARMA Development, production and commercialization of new genomic tools for diagnosis, prognosis and drug-response

More information

DNA. bioinformatics. genomics. personalized. variation NGS. trio. custom. assembly gene. tumor-normal. de novo. structural variation indel.

DNA. bioinformatics. genomics. personalized. variation NGS. trio. custom. assembly gene. tumor-normal. de novo. structural variation indel. DNA Sequencing T TM variation DNA amplicon mendelian trio genomics NGS bioinformatics tumor-normal custom SNP resequencing target validation de novo prediction personalized comparative genomics exome private

More information

an innovation in high throughput single cell profiling

an innovation in high throughput single cell profiling an innovation in high throughput single cell profiling www.dolomite-bio.com Why use high throughput single cell profiling? Techniques such as high throughput scrna-seq (single cell RNA sequencing) offer

More information

Deep Sequencing technologies

Deep Sequencing technologies Deep Sequencing technologies Gabriela Salinas 30 October 2017 Transcriptome and Genome Analysis Laboratory http://www.uni-bc.gwdg.de/index.php?id=709 Microarray and Deep-Sequencing Core Facility University

More information

Pioneering Clinical Omics

Pioneering Clinical Omics Pioneering Clinical Omics Clinical Genomics Strand NGS An analysis tool for data generated by cutting-edge Next Generation Sequencing(NGS) instruments. Strand NGS enables read alignment and analysis of

More information

Detection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System

Detection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System Detection of Rare Variants in Degraded FFPE Samples Using the HaloPlex Target Enrichment System Application Note Author Linus Forsmark Henrik Johansson Agilent Technologies Inc. Santa Clara, CA USA Abstract

More information

ACCEL-NGS 2S DNA LIBRARY KITS

ACCEL-NGS 2S DNA LIBRARY KITS ACCEL-NGS 2S DNA LIBRARY KITS Accel-NGS 2S DNA Library Kits produce high quality libraries with an all-inclusive, easy-to-use format. The kits contain all reagents necessary to build high complexity libraries

More information

RAPID, ROBUST & RELIABLE

RAPID, ROBUST & RELIABLE Roche Sample Prep Solutions for RNA-Seq Sequence what matters RAPID, ROBUST & RELIABLE Sample P le Samp Quant ifi /QC tion ca As the first step in the NGS workflow continuum, sample prep holds the key

More information

Next-generation sequencing technologies

Next-generation sequencing technologies Next-generation sequencing technologies Illumina: Summary https://www.youtube.com/watch?v=fcd6b5hraz8 Illumina platforms: Benchtop sequencers https://www.illumina.com/systems/sequencing-platforms.html

More information

ImmunoID NeXT. Precision Genomics for Immuno-Oncology. ImmunoID NeXT. The Universal Cancer Immunogenomics Platform

ImmunoID NeXT. Precision Genomics for Immuno-Oncology. ImmunoID NeXT. The Universal Cancer Immunogenomics Platform ImmunoID NeXT Precision Genomics for Immuno-Oncology ImmunoID NeXT The Universal Cancer Immunogenomics Platform Personalis, Inc. Advancing Modern Precision Oncology The Universal Cancer Immunogenomics

More information

Corporate Medical Policy

Corporate Medical Policy Corporate Medical Policy Proteogenomic Testing for Patients with Cancer (GPS Cancer Test) File Name: Origination: Last CAP Review: Next CAP Review: Last Review: proteogenomic_testing_for_patients_with_cancer_gps_cancer_test

More information

Next-generation sequencing technologies

Next-generation sequencing technologies Next-generation sequencing technologies NGS applications Illumina sequencing workflow Overview Sequencing by ligation Short-read NGS Sequencing by synthesis Illumina NGS Single-molecule approach Long-read

More information

Single Cell Genomics (SCG): Market Size, Segmentation, Growth, Competition and Trends ( )

Single Cell Genomics (SCG): Market Size, Segmentation, Growth, Competition and Trends ( ) Single Cell Genomics (SCG): Market Size, Segmentation, Growth, Competition and Trends (2013-2021) May, 2017 3 rd Edition Information contained in this market report is believed to be reliable at the time

More information

Satellite Education Workshop (SW4): Epigenomics: Design, Implementation and Analysis for RNA-seq and Methyl-seq Experiments

Satellite Education Workshop (SW4): Epigenomics: Design, Implementation and Analysis for RNA-seq and Methyl-seq Experiments Satellite Education Workshop (SW4): Epigenomics: Design, Implementation and Analysis for RNA-seq and Methyl-seq Experiments Saturday March 17, 2012 Orlando, Florida Workshop Description: This full day

More information

working with scientists to advance single cell research

working with scientists to advance single cell research working with scientists to advance single cell research 4 introduction Why choose Nadia? 6 nadia instrument Nadia Instrument features Why use high throughput single cell profiling? Working with scrna-seq

More information