special offers from your protein biology resource

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2 special offers from your protein biology resource Pop open your cells, extract your proteins, purify, quantify and express them. Seeking knowledge about proteins with Thermo Scientific Protein Research Products is now more cost effective, so you don t need to let your budget hold you back. Now for a limited time, we have special offers on products from cell lysis and sample preparation to immunoprecipitation and purification. Our expanding line of immunoassay products is included in these offers, with discounts in ELISA kits and custom antibody services. Have a look inside and take advantage of the savings now!

3 January - June 2012 Offers! Great products you depend on with offers you ll love. 30% 30% 50% Free Sample! Cell Lysis Reagents Thermo Scientific Pierce Cell Lysis Reagents with Halt Protease Inhibitor Cocktail C18 Spin Columns Thermo Scientific Pierce C18 Spin Columns Protein Concentrators Thermo Scientific Pierce Protein Concentrators Dialysis Tubing Thermo Scientific SnakeSkin Dialysis Tubing Desalting Columns Thermo Scientific Zeba Desalting Columns Free Sample! Free Sample! Special Discount! 20% IP/Co-IP Kits Thermo Scientific Pierce Immunoprecipitation and Co-Immunoprecipitation Kits Chemiluminescent Substrate Thermo Scientific Pierce ECL 2 Substrate Stained and Unstained Protein Ladders Thermo Scientific Pierce Protein Ladders Custom Antibodies Thermo Scientific Pierce Custom Antibodies Target-specific ELISA Kits Thermo Scientific Pierce Target-specific ELISA Kits

4 Protein Concentrators More = Savings Thermo Scientific Pierce Protein Concentrators Pierce Protein Concentrators effectively combine simplicity, speed, capacity and recovery to yield enhanced concentration, purification and separation of proteins even with dilute protein samples. PI-87750BOGO Protein Concentrators, 20K MWCO, 7mL PI-87751BOGO Protein Concentrators, 20K MWCO, 20mL PI-87748BOGO Protein Concentrators, 9K MWCO, 7mL PI-87749BOGO Protein Concentrators, 9K MWCO, 20mL Superior protein concentration and recovery achieve > 110-fold protein concentration in < 35 minutes with > 90% protein recovery Convenient spin formats for 1-20mL of sample Instrument-compatible universal rotor compatibility 2 x 10 concentrators 2 x 10 concentrators 2 x 10 concentrators 2 x 10 concentrators * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. 50% Off when you buy two Offer applies to select Thermo Scientific Pierce Protein Concentrators listed in at left. Dialysis Tubing Thermo Scientific SnakeSkin Dialysis Tubing SnakeSkin Dialysis Tubing is an easy- and ready-to-use form of traditional dialysis membrane tubing that allows desalting and buffer exchange for 10 to 100mL samples. PI-88242PR SnakeSkin Dialysis Tubing, 3.5K MWCO, 16mm Sufficient for approx. 10mL per 5cm length (plus 2.5cm at each end for closure) yields 100 uses 35 ft. (10.5m) PI-88244PR SnakeSkin Dialysis Tubing, 3.5K MWCO, 35mm 35 ft. (10.5m) Sufficient for approx. 48mL per 5cm length (plus 2.5cm at each end for closure) yields 100 uses PI-88243PR SnakeSkin Dialysis Tubing, 10K MWCO, 16mm 35 ft. (10.5m) Sufficient for approx. 10mL per 5cm length (plus 2.5cm at each end for closure) yields 100 uses PI-88245PR SnakeSkin Dialysis Tubing, 10K MWCO, 35mm 35 ft. (10.5m) Sufficient for approx. 48mL per 5cm length (plus 2.5cm at each end for closure) yields 100 uses * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. Off Limited Time Only Convenient ready to use, pre-wetted, pleated tube High recovery > 90% protein recovery Speed dialysis completed in 4-6 hours Stability compatible with a variety of laboratory solutions

5 Desalting Columns Going fast! Thermo Scientific Zeba Desalting Columns Free! sample pack! Simply complete our short survey at pierceoffers, supply your mailing address and select the columns you would like. The complete line of desalting columns, plates and cartridges, with either 7,000 or 40,000 MWCO, contains the proprietary, high performance Zeba Desalting Resin which offers rapid and exceptional protein desalting with high recovery of protein. High recovery low-binding resin maximizes protein recovery Fast no fraction screening or waiting for protein to emerge by gravity flow Easy-to-use no cumbersome column preparation Flexible available in spin columns, filter spin plates and cartridges IP/Co-IP Kits Tag! You save! Thermo Scientific Pierce Immunoprecipitation and Co-Immunoprecipitation Kits Select Kits PI-26180PR Pierce HA-Tag IP/Co-IP Kit Sufficient for 25 IP/Co-IP reactions, each using 10µL of anti-ha resin PI-23620PR Pierce c-myc-tag IP/Co-IP Kit Sufficient for 25 IP reactions, each using 5µL of anti-c-myc resin (5µg anti-c-myc antibody) PI-26149PR Pierce Co-Immunoprecipitation Kit Sufficient 50 Co-IP reactions, each with 25µL of protein A/G resin These Pierce IP/Co-IP Kits enable isolation of native protein complexes without the risk of antibody contamination. Covalently immobilize your primary antibody to an agarose support to prevent leaching and background signal in Western blotting or mass spec analysis. 25- rxn kit 25- rxn kit 50- rxn kit * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. Clean prevent antibody contamination in your Western blots Sensitive use as little as 2µg of antibody per Co-IP Robust re-use immobilized antibodies multiple times Selective anti-ha and anti-c-myc tags available

6 Chemiluminescent Substrate Thermo Scientific Pierce ECL 2 Substrate Stained and Unstained Protein Ladders Thermo Scientific Pierce Protein Ladders Simply complete our short survey at pierceoffers and supply your mailing address for a free sample. Free! 25mL Sample Pierce ECL 2 Substrate is acridanbased, generating acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce strong and sustained chemiluminescence and a robust fluorescent signal at 440nm that can be captured by CCD and fluorescence imagers. High sensitivity detect targets down to the low-picogram level Long signal duration sustained light output for as long as five hours More imaging options X-ray, CCD or laser-based imagers 25µL Samples Free! Simply complete our short survey at pierceoffers, supply your mailing address and select up to TWO protein ladders you would like. Our protein ladder product line offers advanced quality and convenience for all your gel electrophoresis and Western blotting needs. Cost effective high quality without the high price Performance sharp protein-band resolution and consistent migration patterns provide easy molecule determination Convenient protein ladders are ready to load Reliable exceptional lot-to-lot consistency and reproducibility Select from the following ladders: Thermo Scientific PageRuler Prestained Protein Ladder, 25μL (Product # PI-26618) PageRuler Plus Prestained Protein Ladder, 25μL (Product # PI-26621) PageRuler Broad Range Unstained Protein Ladder, 25μL (Product # PI-26631) SuperSignal Molecular Weight Protein Ladder, 25μL (Product # PI-84785S)

7 Custom Antibodies Thermo Scientific Pierce Custom Antibodies Target-specific ELISA Kits Target savings! Thermo Scientific Pierce Target-specific ELISA Kits The Pierce Custom Antibody Service provides high-quality customized products and services for your protein characterization and detection needs. Generate robust antibodies for the detection of endogenous protein in Western blot, immunoprecipitation (IP), immunohistochemistry (IHC), immunofluorescence (IF) and ELISAs. greater than Off PI-HAB SPF rabbit 90-day Peptide Antibody Includes: Advanced peptide design with Thermo Scientific Antigen Profiler Software Targeted Antigen Display Conjugation to KLH or similar carrier Immunization of two specific pathogen-free rabbits Four boosts per rabbit, including primary boost in CFA Three post-inject bleeds per rabbit for a total of ~60mL serum per rabbit Pre-immune control bleeds from each rabbit 5mg of purified peptide High-performance liquid chromatography (HPLC) and mass spectrometry on peptide ELISA titration on all bleeds upon protocol completion to monitor the immune response Complete online project tracking using Open Project software Optional peptide affinity purification PI-HAB SPF rabbit 70-day Supplied Protein Antibody Includes: Targeted Antigen Display Conjugation to KLH or similar carrier (if needed) Immunization of two specific pathogen-free rabbits Four boosts per rabbit, including primary boost in CFA Three post-inject bleeds per rabbit for a total of ~60mL serum per rabbit Pre-immune control bleeds from each rabbit ELISA titration on all bleeds upon protocol completion to monitor the immune response * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. Off Select Kits Pierce Target-specific ELISA Kits, Screening Sets and MiniKits provide all or some of the reagents for standard 96-well microplate assays for quantitative measurement of individual cytokine, chemokine and other markers using the enzyme-linked immunosorbent assay (ELISA) technique. A variety of immoassay kit configurations are available for numerous sample types. * Offer valid January 1, 2012 through June 30, Not valid with other offers and discounts.

8 Cell Lysis Reagents High-yield savings Thermo Scientific Pierce Cell Lysis Reagents with Halt Protease Inhibitor Cocktail 30% Kit PI-78248BNDL B-PER Plus Halt Protease Inhibitor Cocktail Kit Includes: B-PER Reagent Halt Protease Inhibitor PI-78501BNDL M-PER Plus Halt Protease Inhibitor Cocktail Kit Includes: M-PER Reagent Halt Protease Inhibitor PI-78510BNDL T-PER Plus Halt Protease Kit Inhibitor Cocktail Kit Includes: T-PER Reagent Halt Protease Inhibitor 500mL 1mL Kit 250mL 1mL 500mL 1mL * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. Pierce Cell Lysis Reagents provide gentle whole cell lysis buffers for high-yield protein extraction and solubilization. Combined with the ready-to-use Halt Protease Inhibitors that contain six broad-spectrum inhibitors, you have everything needed to protect your proteins. Kits available: Thermo Scientific B-PER Plus Halt Protease Inhibitor Cocktail For E. coli and other bacterial cells Thermo Scientific M-PER Plus Halt Protease Inhibitor Cocktail Kit For cultured mammalian cells Thermo Scientific T-PER Plus Halt Protease Inhibitor Cocktail Kit For soft tissue samples C18 Spin Columns Savings you can C Thermo Scientific Pierce C18 Spin Columns Pierce C18 columns help clean up and concentrate multiple peptide samples for mass spectrometry in less than 30 minutes. 30% PI-89870PR Pierce C18 Spin Columns Kit 25 columns each containing 8mg of a porous C18 reversed-phase resin. * Offer valid January 1, 2012 through June 30, Must use special Product # listed here to receive this price. Not valid with other offers and discounts. Removes MS-interfering contaminants significantly reduces signal suppression and improves signal-to-noise ratios Robust works with a wide variety of load volumes and concentrations Convenient requires no special equipment for processing multiple samples simultaneously Sensitive special C18 resin allows excellent recovery percentages, even at sub-picomole sample loads

9 Select from these popular Pierce Target-specific ELISA Kits, s and MiniKits and receive off. Product # ESS0026BPR ESS0017PR EHIFNGPR EHIFNG2PR EHIFNG5PR ESS0002PR KHIFNGPR PI-84776PR EH3TNFAPR EH3TNFA2PR EH3TNFA5PR Description Bovine IFN gamma Equine TNF alpha Human IFN gamma Colorimetric ELISA Human IFN gamma Colorimetric ELISA Human IFN gamma Colorimetric ELISA Human IFN gamma Human IFN gamma ELISA MiniKit 40 plate Chemiluminescent ELISA Kit Product # EHVEGFPR ESS0001PR KHTNFAPR KMTNFAPR EHVEGF2PR EHVEGF5PR EPIFNGPR EP2TNFAPR ESS0003PR ERIFNGPR ERIFNG2PR Description Human VEGF Colorimetric ELISA Kit ELISA MiniKit Mouse TNF alpha ELISA MiniKit Human VEGF Colorimetric ELISA Kit Human VEGF Colorimetric ELISA Kit Porcine IFN gamma Porcine TNF alpha Porcine TNF alpha Rat IFN gamma Rat IFN gamma 40 plate 40 plate Product # ER3TNFAPR ER3TNFA2PR ER3TNFA5PR EM1001PR EM10012PR EM10015PR EMTNFAPR EMTNFA2PR EMTNFA5PR ESS0020PR KMIFNGPR Description Rat TNF alpha Rat TNF alpha Rat TNF alpha Mouse IFN gamma Mouse IFN gamma Mouse IFN gamma Mouse TNF alpha Mouse TNF alpha Mouse TNF alpha Mouse IFN gamma Mouse IFN gamma ELISA MiniKit 40 plate Special Offer!

10 Speak up! share your knowledge write a review! go to our website Thermo Scientific Pierce Product Blog New online repository for product news and technical articles The Pierce Product Blog contains recent new products and journal-style articles covering Thermo Scientific Protein Products methods, claims and applications. The new format provides increased functionality, including immediate access to related product pages and the opportunity to interact with authors via comments. Visit today and subscribe to an RSS feed. See what people are saying about our products and tell us what you think. We'd like to hear about your experiences with Thermo Scientific Pierce Protein Research Products. Writing a review gives you a chance to voice your opinion, share your knowledge and helps other researchers make informed choices. You can also help make our products better and influence the design of future products. For more special offers, visit In the United States: For customer service, call To fax an order, use To order online: In Canada: For customer service, call To fax an order, use To order online: BN /12 Printed in the U.S.