Sartobind SingleSep IEX Membrane Adsorber Capsules

Transcription

1 Sartobind SingleSep IEX Membrane Adsorber Capsules

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3 Sartobind Capsules for Economic Production of Therapeutic Proteins High speed polishing Sartobind SingleSep capsules are designed to remove charged contaminants from therapeutic proteins at accelerated flow rates by ion exchange membrane chromatography. The high throughput is a direct result of negligible mass transfer effects and is made possible by the >3 µm macroporous membrane with 4 mm (15 layer) bed height. Approved technology In 2001 Campath * received FDA approval. This monoclonal antibody is polished by application of Sartobind ion exchange Q modules in flow through mode (DNA endotoxin leached protein A virus removal). It was the first time that a Membrane Adsorber has been accepted in the production of a therapeutic protein. This shows not only Sartorius position as the pioneer in adsorptive membrane chromatography but also displays that this technology is proven and tested. * Campath is a trademark of ILEX Pharmaceuticals Efficient Higher throughput (g/h) for trace impurity removal Economical Saves capital No hardware investment & maintenance No column packing, testing, regeneration No re-use validation Less unspecific binding higher yield Less labor Buffer consumption may be decreased 95% Easy to use Disposable Simple and fast set up Handling like a filter capsule Controlled quality Sartobind SingleSep capsules are designed, developed and manufactured in accordance with an ISO 9001 certified Quality Management System. They have passed Plastic Class VI, particles and extractables test according to current United States Pharmacopoeia (USP) and are tested for protein binding capacity prior to release. Each package contains a certificate of quality. A validation guide is available upon request: Order No.: IEX gel column Materials Labour Consumables Hardware costs IEX Membrane Adsorber Capsule 80% less Materials Labour Consumables Fig. 2: Cost comparison of columns and Membrane Adsorbers for polishing application Capsule design The Sartobind SingleSep looks like a standard filter capsule except that the adsorbing membrane is reeled on a core to form a cylinder. The flow is from top into the outside channel and then perpendicular through the membrane layers to the center of the cylinder and leaves the capsule at the outlet. Flow path/ 4 mm bed height Sartobind capsule Fig. 3: Schematic flow pattern and cross section of a 10 SingleSep capsule l Fig. 1: Sartobind 30 inch capsule connected to TC Tech Sartorius disposable bag. 3

4 Application Area In a generic downstream process for the production of a monoclonal antibody, the main capturing steps are followed by the polishing step(s). The later the anion exchanger is applied in the process, the less contaminants have to be removed. Sartobind SingleSep in monoclonal antibody production: Cell removal Clarification Affinity Chromatography Cation Exchange Chromatography Anion Exchange Adsorber Polishing Step (Flow Through): Removal of DNA, HCP, leached Protein A, Endotoxins Virus Clearance Ultra- Diafiltration + The positively charged ligand of the Q adsorbers binds negatively charged contaminants. Neutral or positively + charged product runs through (see Figure). Endotoxin Protein A Host cell protein + DNA Virus MAb + Fig. 4: Isoelectric points and charges of MAbs and impurities ph of buffer Binding capacity on Sartobind strong ion exchanger membranes IEX ligand Type of molecule Binding capacity Binding capacity Ref. chemistry per ml per cm 2 Q BSA >29 mg >0.8 mg 1 Q DNA 5.6 mg 0.16 mg 2 Q Endotoxin EU EU 3 Q Adenovirus up to up to Viral Particles 4 S Lysozyme >29 mg >0.8 mg ml = 36.4 cm 2 IEX membrane 1 Sartorius quality control for minimum static binding capacity 2 J. K. Walter, (Boehringer-Ingelheim Pharma) in: Bioseparation and Bioprocessing, G. Subramanian (ed.) Wiley VCH, 1998, Vol. II p M. Belanich et al., Reduction of Endotoxin in a Protein Mixture Using Strong Anion Exchange Membrane Adsorption, Pharm. Technology 03, 1996, p Measured with Vivapure AdenoPack 500. Order no.: VS-AVPQ501

5 Virus clearance This study 5 also demonstrates excellent virus log reduction values with Sartobind Q. This enables membrane adsorber technology to replace a Q column without compromising critical viral clearance capabilities. Fig. 5: Noize exclusion effects for viruses Murine Pseudorabies Reo Virus Minute Virus Leukemia Virus type 3 of Mice Virus (MuLV) (PRV) (Reo-3) (MVM) 50 ml virus > 5.57 ± 0.25 > 5.67 ± 0.17 > 7.28 ± 0.30 > 6.77 ± 0.24 spiked protein sample 500 ml virus 6.29 ± 0.25 > 5.67 ± 0.23 > 7.53 ± ± 0.37 spiked protein buffer solution 5 R. Zhang (Abgenix Inc.) et al, Viral Clearance Feasibility Study with Sartobind Q Membrane Adsorber for Human Antibody Purification, BioProduction 2004, Munich Germany October Capsules vs. Columns The use of conventional chromatography columns for flow through (FT) anion exchange chromatography requires high flow rates. Optimized production columns need a certain diameter and bed height to achieve a large throughput and therefore have a large bed volume. That is the reason why columns are typically oversized. reduced by about 80% and buffer reduction can reach 95%. Protein loading in FT can be two orders of magnitude higher than with columns 6, 7. Fig. 6: Oversized column compared to Sartobind SingleSep for polishing application Due to the cylindrical construction of the adsorber the high flow rate of the membrane is combined with a large frontal surface resulting in very high throughput and keeping the bed volume small. Typical process data show that operation time can be Comparison of Q packed bed chromatography and Q Membrane Adsorber 6 Q Resin Q Membrane Adsorber Flow rate cm/h cm/h Protein loading (flow through) g/l >3,000 g/m 2 or >10.9 kg/l Buffer used 100% 5% Cleaning validation Yes No 6 J.X. Zhou, T. Tressel (Amgen Inc.), Membrane Chromatography as a Robust Purification System for Large-Scale Antibody Production, BioProcess Int. 09, 2005, p H. Knudsen et al. Membrane Ion Exchange Chromatography for Process-Scale Antibody Purification, J. Chromatography A 907, 2001,

6 Technical data Membrane Base material Membrane thickness 275 µm Stabilized reinforced cellulose Pore size (nominal) >3 µm Ion exchanger types strong anion Q (quaternary amine) R-CH 2 -N + (CH 3 ) 3 - strong cation S (sulfonic acid) R-CH 2 -SO 3 Fig. 7: SingleSep mini and SingleSep 5 Capsule materials Outer cage, inner core, end caps, capsule housing, nonwovens O-ring in vent valve* Bed height * only with 10, 20 and 30 capsules Polypropylene Silicone 4 mm (15 membrane layers) Operation Depyrogenation 1 N NaOH one hour Autoclaving 121 C for 30 minutes for one cycle only Maximum operating pressure 4 bar, 0.4 MPa, 58 psi Maximum operating temperature 80 C, 176 F Dimensions and connections Type of capsule SingleSep mini SingleSep 5 SingleSep 10, 20, 30 Sanitary flange version Capsule (height+width) mm mm mm (10 ) mm (20 ) mm (30 ) Inlet connector 25 mm outer d 50.5 mm outer d 50.5 mm outer d 10 mm inner d 15 mm inner d 36 mm inner d Outlet connector as above 50.5 mm outer d as above 19 mm inner d Hose barb version Capsule (height+width) mm mm n. a. Hose barb connectors 6 12 mm 14.5 mm n. a. Recommended internal diameter 6 10 mm 12 mm n. a. of flexible tube Related products are Sartobind MA units for laboratory use and the Sartobind MultiSep reusable modules. n.a. = not available 6

7 Ordering Information Sartobind Q SingleSep capsules Order no. Chemistry Description Quantity Bed Bed Nominal Frontal Protein Recomadapter inlet volume height membrane surface* binding mended and outlet [ml] [mm] area area capacity** flow rate [cm 2 ] [g] [l/min] Sartobind mini 92IEXQ42D4-OO--A Q hose barb cm IEXQ42D4-SS--A Q sanitary flange cm Sartobind 5 inch 92IEXQ42D9-OO--A Q hose barb ,500 cm IEXQ42D9-SS--A Q sanitary flange ,500 cm Sartobind 10 inch 92IEXQ42D1-SS Q sanitary flange ,600 cm Sartobind 20 inch 92IEXQ42D2-SS Q sanitary flange m Sartobind 30 inch 92IEXQ42D3-SS Q sanitary flange m 2 1, Sartobind S SingleSep capsules Sartobind mini 92IEXS42D4-OO--A S hose barb cm IEXS42D4-SS--A S sanitary flange cm Sartobind 5 inch 92IEXS42D9-OO--A S hose barb ,500 cm IEXS42D9-SS--A S sanitary flange ,500 cm Sartobind 10 inch 92IEXS42D1-SS S sanitary flange ,600 cm * Frontal surface area = average cylinder surface ** Binding capacity was measured with BSA, for Q and lysozyme for S chemistry Accessories Order no. 5ZGI ZGLG ZALB-0002 Description Stainless steel holder for one 10, 20 or 30 inch capsule, 3 legs Stainless steel holder for three 10, 20 or 30 inch capsules, 3 legs Stainless steel distribution adapter for three 10, 20 or 30 inch capsules Fig. 8: A SingleSep 20 capsule on stailess steel holder 7

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