Standard Operating Procedure for performing a muscle biopsy
|
|
- Victoria Williamson
- 6 years ago
- Views:
Transcription
1 Standard Operating Procedure for performing a muscle biopsy Effective date: Review due date: Original Author Name: Richard Metcalfe and Abdullah Alghannam Date: Position: Ph. D. Students Reviewer Name: Ben Lee Signature: Position: Physiology Lab Technician Date: Approved by Name: Prof Keith Stokes Signature: Position: Deputy Head of Department Date: Amendment Chronology Version number Effective date Reason for amendment Reviewed/Amended by First issue Richard Metcalfe and Abdullah Alghannam No amends Ben Lee Minor changes to procedure and autoclaving. Appendices removed. Mark Thomas Pippa Heath Version: 2 Effective date:
2 Performing a Muscle Biopsy Safety Information: Prior to performing this procedure please refer to the following risk assessment: Purpose: muscle_biopsy The purpose of the muscle biopsy technique is to obtain a sample of muscle tissue for research purposes. Decontamination of biopsy equipment involves the destruction or removal of any micro-organisms present and is necessary to prevent cross-contamination or infection of individuals. Cleaning, disinfection and sterilisation are used in the decontamination process to maintain sterility, integrity and prolong the life-span of the equipment. Contents: Only individuals who have undergone the required training, and read and understood the risk assessment, may perform this procedure. Best practice must be adhered to at all times. 1. Equipment Required 2. Preparation 3. Taking a sample 4. Decontamination and sterilisation 5. Ordering Information Equipment Required: Materials for Performing the Biopsy Scalpel and Blade (Swann morton, stainless steel, size 11) Gauze Converter Sterile Gloves Biopsy (Bergstrom) Needle Anaesthetic (Lidocaine) Iodine (Videne) & Iodine reservoirs Steri Strips 2 x 5ml syringe 100ml syringe Version: 2 Effective date:
3 Catheter tubing Needle (orange) or grey 27 2 x Needle (green) 21 Forceps Sterile Scissors Regular Scissors Coban Tape Sharps bin Hazardous waste bin Liquid nitrogen canister & liquid nitrogen Razors Autoclave bags Spatula Adhesive Island Dressings (Primapore) Eppendorf safe-lock tubes. Medical tweezers (fine with curved tips) Stitches Small metal bowl for liquid nitrogen (optional) Materials for Decontamination Bergstrom Biopsy Needles Metal Scissors Metal Forceps Sterilising Pouch Autoclave Indicator Strip Autoclave Validation Vial (Sterikon Plus Bioindicator VWR Order number ) Ultrasonic Bath (2.5 L capacity) Basket for biopsy equipment Sonozyme Enzymatic Detergent Milton Sterilising Solution Ultra-pure water Wire Brush Preparation: 1. Sterilised biopsy needle in autoclave bag 2. Fill canister with liquid nitrogen 3. Attach catheter to 100ml syringe in preparation for needle. Cut the tube in a sharp angle. 4. With green needle poke 3 holes in top of eppendorf ready for liquid nitrogen + sample Taking a sample: 1. Prepare a sterile tray by placing a sterile sheet. Version: 2 Effective date:
4 2. Place the reservoir and Videne on the sterile tray. 3. Shave the lateral aspect of the thigh to prepare the biopsy area. 4. Request subject to tense their quadriceps to establish the anatomical location for the incision (approximately 45 o from standing). 5. Sanitise hands, put sterile gloves on 6. Apply Videne on the entire visible area of the thigh through an outward circular motion for 30 seconds. Allow to dry naturally and mark site with fingertip 7. Load two 2.5ml syringes with anaesthetic (Lidocaine), expel air bubbles outside bottle. Without touching either needle, place a grey 27 needle and a green 21 needle onto each of the syringes. 8. Insert the grey needled syringe in a shallow angle to penetrate the skin; administer the anaesthetic upwards towards the (circle as a fan shape). 9. Insert the green needled syringe vertically in one motion (relatively quickly) to feel the facia (subtle increase in resistance should be felt); Continue to administer the anaesthetic on the facia (circle as a 2 pence piece). 10. Wait for 2 minutes. 11. Ensure that the subject has no pain sensations before commencing to the next step (confirm with the subject). 12. Insert blade vertically, and cut along skin to reach the fascia. Continue with a backward and forward motion, while twisting the blade in the end of the incision to allow the opening of the facia. 13. Flood with Videne and place pressure with gauze on top 14. Open biopsy needle autoclave bag 15. Attach needle to 100ml tube by the catheter tube. 16. Insert biopsy needle vertically, twisting as you insert and locate notch in facia 17. Apply some pressure by moving laterally from the femur. Ensure that the window in the needle is also facing laterally. 18. Expel air from needle and hold horizontally before performing suction. 19. Pull needle up, pull syringe out and snip into muscle (2-3 times). Rotate 1/8 turn in between each snip. 20. Remove needle and flood wound area with Videne and place pressure with gauze on top. 21. Use one stitch to close the wound. 22. Seal wound area with steri-strip and place Adhesive Island Dressing. 23. Apply pressure with gauze for at least 5 minutes. Wrap with Coban tape for pressure. Version: 2 Effective date:
5 24. Decant muscle into eppendorf of liquid nitrogen. Participant care: Instruct the participant on maintenance of biopsy site and how to prevent infection. The stitch will need to be removed in 5-7 days. Coban tape needs to remain on for a minimum of 2 hours, but ideally 24 hours to reduce bruising risk. Provide them with extra Primapore. Decontamination and Sterilisation: Materials Bergstrom Biopsy Needles Metal Scissors Metal Forceps Sterilising Pouch Autoclave Indicator Strip Autoclave Validation Vial (Sterikon Plus Bioindicator VWR Order number ) Ultrasonic Bath (2.5 L capacity) Basket for biopsy equipment Sonozyme TM Enzymatic Detergent Pre-sept Sterilising Solution Ultra-Pure Water Wire Brush Procedure: 1. Wash all biopsy equipment: Bergstrom needles (all component parts are separated and cleaned individually) in warm water and physically remove any organic matter, blood and other deposits with a soft nylon bristled brush. Ensure the brush is cleaned with detergent and hot water directly after use. 2. Place the items in an ultrasonic bath containing a solution of 0.3% Sonozyme enzymatic soap (6.25mls per 2 litres of water) and sonicate for minutes at 30 o C. If decontaminating a large batch of biopsy equipment, a maximum of five items should be placed in the basket and treated at any one time. Make sure items do not overlap in the Sonicator. 3. Rinse all items in water. 4. Soak the items in a suitable disinfectant i.e Pre-sept sterilising solution for fifteen minutes and rinse in ultrapure water. Version: 2 Effective date:
6 5. Dry items with a blue towel and place individually in sterilising pouches. Seal with autoclave tape and label the tape with the item name, Health, the lab, your initials and the date in permanent ink. 6. Place in Biology autoclave room on dry shelf. End of Document Version: 2 Effective date:
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Biomaterial Hemolytic Assay. Kit Insert
COMPANY WITH QUALITY MANAGEMENT SYSTEM CERTIFIED BY DNV GL = ISO 9001 = Biomaterial Hemolytic Assay Kit Insert Version: April 2017 Summary Hemolytic activity is a requirement to be tested for any blood
More informationTRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE
TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE
More informationLABORATORY WASTE MANAGEMENT PROGRAMMES
LABORATORY WASTE MANAGEMENT PROGRAMMES Sylvia Wanjiru Kamau INTERNATIONAL LIVESTOCK RESEARCH INSTITUTE LABORATORY MANAGEMENT AND EQUIPMENT OPERATIONS WORKSHOP. 17 TH -21 ST JUNE 2012 TRAINING OBJECTIVES
More informationBovine IgG ELISA Kit
Bovine IgG ELISA Kit Cat. No. E11-118 Components Supplied Bovine IgG Pre-Coated 96-well Strip Plate, 1 each Bovine IgG Standard, 500 ng/vial, 2 each Bovine IgG Detection Antibody, 12 ml 20X Dilution Buffer
More informationPROGRAM FOR THE MANAGEMENT AND DISPOSAL OF RADIOACTIVE WASTE
RADIATION SAFETY DEPARTMENT West Virginia University Health Sciences Center WVU Hospitals Jefferson Memorial Hospital G-139 Health Sciences Center PO Box 9006 Morgantown, WV 26506-9006 Phone: 304-293-3413
More informationASEPTIC TRANSFER & PURE CULTURE TECHNIQUES
ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES GENERAL GUIDELINES & REMINDERS: SAFETY: NO EATING OR DRINKING IN THE LAB! Wash your hands with soap both BEFORE and AFTER lab, and, in addition, when you have
More informationRegulated Medical Waste Management and Proper Waste Segregation. State-Specific Information New Jersey
Regulated Medical Waste Management and Proper Waste Segregation State-Specific Information New Jersey Identifying & Segregating Regulated Medical Waste RMW in New Jersey Six Categories of Regulated Medical
More informationPRODUCT CATALOGUE. for gke Steri-Record and gke Clean-Record Chemical indicators, PCDs and accessories for cleaning and sterilization
PRODUCT CATALOGUE for gke Steri-Record and gke Clean-Record Chemical indicators, PCDs and accessories for cleaning and sterilization Bowie-Dick Simulation Test Batch and Process Monitoring Systems, Documentation
More informationL'ISOLANTE K-FLEX. LNG applications Manual
L'ISOLANTE K-FLEX LNG applications Manual LNG applications Manual The insulation LNG plants may forsee a multilayer design according to the specific characteristics and the working temperatures of the
More informationGuide for Cleaning, Sterilization and Storage of Introducers for Caldera Medical Implants
Guide for Cleaning, Sterilization and Storage of Introducers for Caldera Medical Implants M Manufactured by: Caldera Medical, Inc. 5171 Clareton Drive Agoura Hills, CA 91301 U.S. Toll Free: 866-4-CALDERA
More informationUNIVERSITY OF TOLEDO HEALTH SCIENCE CAMPUS
UNIVERSITY OF TOLEDO HEALTH SCIENCE CAMPUS SUBJECT: SPECIMEN TRANSPORT IN Procedure No: S-08-011 COMPUTERIZED TUBE SYSTEM PROCEDURE STATEMENT All laboratory specimens will be transported in a manner consistent
More informationBiomedical Waste Management Plan
Biomedical Waste Management Plan USF Biomedical Waste Management Plan for: Facility Address: Facility Phone Number: Facility Contact: Division of Environmental Health and Safety 4202 E. Fowler Ave, OPM
More informationCAUTION: FEDERAL LAW (USA) RESTRICTS THESE DEVICES TO SALE BY OR ON THE ORDER OF A PHYSICIAN.
Manufacturer Silony Medical GmbH Leinfelder Strasse 60 70771 Leinfelden-Echterdingen Germany US Representative: Silony Medical Corp. 8200 NW 27TH STR, STE#104, DORAL, FL 33122 USA Phone: +1 305 916 0016
More informationInstructions for use. Reusable surgical instruments. Hersteller:
Hersteller: ROLAN Instruments GmbH Ernst-Thälmann-Str. 50 15859 Storkow / Germany Tel.: 0049 33678 449433 E-Mail: info@rolan-instruments.de Item codes 01-02- 03-04- 05-06- 07-08- 09-10- 11-12- 13-14- 15-16-
More informationPolicies Concerning Survival Surgery of Mice
Policies Concerning Survival Surgery of Mice Date approved: October 25, 2016 Purpose This document details the minimum standards for survival (recovery) surgery in all laboratory rodent species. Responsibility
More informationHealth Care Waste Management Training. Resource Guide
Training Resource Guide 5 August 2011 Training Resource Guide 5 August 2011 Scott Ackerson SCMS sackerson@pfscm.org Acknowledgements The authors of this report would like to acknowledge the assistance,
More informationMaterial List by Procedure. The following lists are provided to ensure that the correct supplies are in place prior to the procedure.
Material List by Procedure Purpose: Policy: The following lists are provided to ensure that the correct supplies are in place prior to the procedure. Changes to material lists must be approved by the medical
More informationOne such problem is blood residual inside the channels of a robotic arm.
SUBJECT: Robotic Arm Testing for residue blood DEPARTMENT: Central Service APPROVED BY: EFFECTIVE: REVISED: 07/2016 PURPOSE: To test for detection of blood residue inside and outside of a robotic arm and
More informationBest Practices for Reprocessing Endoscopes
Best Practices for Reprocessing Endoscopes Reprocessing of Endoscopes Reprocessing Room Standards The resources referenced include: Canadian Standards Association Provincial Infectious Disease Advisory
More informationYou are required to complete this course with a passing score of 85% or higher if you:
OHS Biosafety BIO301L Medical Waste Management for Labs Training You are required to complete this course with a passing score of 85% or higher if you: Offer medical waste for transport from a UAB campus
More information4.1 DETERMINATION OF SPECIFIC GRAVITY
TESTS ON CEMENT 94 4.1 DETERMINATION OF SPECIFIC GRAVITY STANDARD IS: 4031-1988. DEFINITION Specific Gravity is defined as the ratio of the mass of the cement to the mass of an equal volume of kerosene.
More informationBIOMEDICAL WASTE MANAGEMENT POLICY
BIOMEDICAL WASTE MANAGEMENT POLICY Managing biomedical waste in a safe and environmentally responsible manner is a key focus of the Environmental Compliance and Sustainability office. 1.0 PURPOSE In an
More informationSTERILIZATION ON MSF EMERGENGY SETTING. Pascale CHOQUENET Médecins Sans Frontières Paris France Milan 5th June 2008
STERILIZATION ON MSF EMERGENGY SETTING Pascale CHOQUENET Médecins Sans Frontières Paris France Milan 5th June 2008 MSF = Doctors Without Borders International Humanitarian Organisation Medical NGO (Non
More informationHemoglobin HemoCue Hb method
TLM_PRO_027 and Version No. 001 Page 1 of 5 Department: Clinical Sciences Unit: Tropical Laboratory Medicine Hemoglobin HemoCue Hb 201 + method Project/study: Not applicable 1. Scope and application This
More informationRevised 27 Aug rm (Vers. 4.1)
Please use only the valid version of the package insert provided with the kit. INTENDED USE Vitamin Folic Acid Test is a microtiter plate test kit based on a microbiological assay which measures the total
More informationSEB Autoclave Training
SEB Autoclave Training 1 Autoclave Details 3 Autoclaves located in SEB, one on each upper floor. Expensive pieces of equipment ($70,000 each). Essential for several groups research. Most malfunctions are
More informationAseptic Techniques. A. Objectives. B. Before coming to lab
Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note
More informationUniversity Health Services Health and Safety
ADVISORY NO. 10.2: MANAGEMENT OF BIOLOGICAL AND INFECTIOUS WASTES The following recommendations are based on the Ohio Revised Code (ORC), Ohio Administrative Code (OAC) and EPA guidelines regarding biological
More informationTitle: Hemoglobin HemoCue Hb 301 method
TLM_PRO_028 and Version No. 001 Page 1 of 5 Department: Clinical Sciences Unit: Tropical Laboratory Medicine Title: Hemoglobin HemoCue Hb 301 method Project/study: not applicable 1. Scope and application
More informationDisposal of biological waste from containment level 2 laboratories in the WIMM
Disposal of biological waste from containment level 2 laboratories in the WIMM This document describes how biological waste from containment level 2 laboratories in the WIMM is managed. Written by Malcolm
More informationMethods for obtaining Batrachochytrium dendrobatidis (Bd) samples for PCR testing
Methods for obtaining Batrachochytrium dendrobatidis (Bd) samples for PCR testing By Lauren J. Livo Department of Integrative Physiology, University of Colorado, Boulder, CO 80309-0354 General considerations
More information32 Sq ft Kit Technical Data Sheet
HYDROCHROME 32 SQ FT KIT 32 Sq ft Kit Technical Data Sheet INTRODUCTION Hydrochrome is a very versatile product that, once applied, produces a 100% mirror effect which, will not dull down under a 2K topcoat.
More informationIntravitreal and sub-retinal injections of plasmid DNA and electroporation in P0 pups
Intravitreal and sub-retinal injections of plasmid DNA and electroporation in P0 pups Protocol modified from: Retinal Gene Delivery by raav and DNA Electroporation, Aditya Venkatesh et all, Current Protocols
More informationDepartment of Microbiology, Lab 016 instructions
Protocol for standard FISH and DOPE-FISH for prokaryotes (slightly modified from Amann, 1995, note, for other modifications or other microorganisms like eukaryotes, consult special literature or check
More informationMarine Fire Wrap General Installation Instructions Cable Penetrations through A0-A60 and B0-B30 Bulkheads and Decks
Marine Fire Wrap General Installation Instructions Cable Penetrations through A0-A60 and B0-B0 Bulkheads and Decks Reference(s) MED 1050201, EC Type Examination Module B (Lloyd s Register, LR) F-20427
More informationTWO-DIMENSIONAL GEL ELECTROPHORESIS THE FIRST DIMENSION
TWO-DIMENSIONAL GEL ELECTROPHORESIS THE FIRST DIMENSION This procedure has been developed for the Hoefer DE 102 series tube gel electrophoresis apparatus, which can run up to 12 tube gels at a time. The
More informationHistological preparation of embryonic and adult zebrafish eyes
Histological preparation of embryonic and adult zebrafish eyes Richard J. Nuckels 1 and Jeffrey M. Gross 1,2,3 1 Section of Molecular Cell and Developmental Biology 2 Institute of Cell and Molecular Biology
More informationAnti- Tissue Transglutaminase TTG IgG ELISA Kit
Anti- Tissue Transglutaminase TTG IgG ELISA Kit Catalog number: NR-R10142 (96 wells) The kit is designed to quantitatively detect TTG IgG in Human serum or plasma. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC
More informationProcedure for Health Care Risk Waste Management. Procedure No. 403
Procedure for Health Care Risk Waste Management Procedure No. 403 Print Name Title Date Prepared by J.G. MacNamara T.S.O. 01/11/04 Reviewed by J. Hoare CATSO 01/11/04 Corporate Authorisation J.G. MacNamara
More informationGuidelines for QualiTru Line Sampling
Guidelines for QualiTru Line Sampling QUALITRU GUIDELINES FOR LINE SAMPLING 1. Purpose: Following these Standard Operating Procedures, farms and processing plants may use the QualiTru aseptic sampling
More informationProducts and Guidelines for Enhanced Office Safety
infection prevention IMS WRAP SporeCheck STERILIZATION solutions Products and Guidelines for Enhanced Office Safety Bagette pouches sterilization monitoring CDC recommends placing an indicator inside each
More informationThermo Scientific. Fiberlite Rotors. for the Thermo Scientific Sorvall LYNX Centrifuge. Instruction Manual a 09 / 2014 Superspeed Centrifuge
Thermo Scientific Fiberlite Rotors for the Thermo Scientific Sorvall LYNX Centrifuge Instruction Manual 50141693-a 09 / 2014 Superspeed Centrifuge WEEE Compliance This product is required to comply with
More informationSafe Operating Procedure
Safe Operating Procedure (Revised 3/17) AUTOCLAVE OPERATION AND PERFORMANCE TESTING Autoclaves are used in many areas to sterilize materials. Due to the high heat and pressure created in autoclaves during
More information****** Competition ELISA Kit Instruction
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC PURPOSES ****** Competition ELISA Kit Instruction Kit name and catalog number Your analyte ELISA Kit, Catalog#: ***** Intended use The kit is used to detect the
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationLAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA
LAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA I. Objectives The purpose of today s lab is to learn how to set up and run an agarose gel, separate DNA fragments on the gel, and
More informationHuman DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure
Human DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure *Polymerase Chain Reaction is covered by patents owned by Hoffmann-La Roche, Inc. This experiment was adapted from Laboratory
More informationGeneration of Mouse Spinal Cord Injury Oneil G. Bhalala *, Liuliu Pan, Hilary North, Tammy McGuire and John A. Kessler
Generation of Mouse Spinal Cord Injury Oneil G. Bhalala *, Liuliu Pan, Hilary North, Tammy McGuire and John A. Kessler Department of Neurology, Northwestern University, Chicago, USA *For correspondence:
More information3M TM Decorative Radiant Film 76929SR
SR Product Data Sheet Product Description Date: May 2010 Supercedes : New 3M Decorative Radiant Film 76929SR is a transparent decorative pressure sensitive adhesive film incorporating 3M Radiant Light
More informationOPERATING INSTRUCTIONS
Water Sampler # 15010 OPERATING INSTRUCTIONS Purpose: Contents: For the collection of water samples at varying depths or distances away from a shoreline. One (1) Assembled Water Sampler One (1) Cord (15
More informationSTANDARD OPERATING PROCEDURE
Effective Date: 12/1/2011 Page 1 of 12 Approval Name/Title Signature Date Authors: Jessica Landis Trial Implementation Manager Immune Tolerance Network Mary C. Philogene Associate Director, QA And Cell
More informationExperiment 3: The Chromatography of Organic Compounds
Experiment 3: The Chromatography of Organic Compounds INTRODUCTION Very often, in an organic synthesis, a reaction will proceed to produce multiple products or perhaps will only partially form the desired
More informationTo whom it may concern, February 21, 2011
To whom it may concern, February 21, 2011 The following is an evaluation of the GEMS grant that I received to start my own C-MORE lab kits, specifically ocean acidification, plankton, marine debris and
More informationHuman immunoglobulin G(IgG) ELISA Kit
Human immunoglobulin G(IgG) ELISA Kit For the quantitative determination of human immunoglobulin G (IgG) concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates.
More informationSpecialty Needles and Surgical Products Range Hill-Rom is a leading global medical technology company with more than 10,000 employees in over 100 countries. We partner with health care providers by focusing
More information1 Testing Equipment. Laboratory General Sampling Sample Reduction Sieve Analysis Decantation Deleterious and Chert
1 Testing Equipment Laboratory General Sampling Sample Reduction Sieve Analysis Decantation Deleterious and Chert Test Equipment Verification Laboratory Set-Up 1 CHAPTER ONE: TESTING EQUIPMENT LABORATORY
More informationSterilization of Schweickhardt Instruments
Sterilization of Schweickhardt Instruments INTRODUCTION l Most dental instruments are made of corrosion-resistant highgrade steels. The requirements for grades of steel are defined in national and international
More informationFinal Approval: October 2010 Effective: October 2010 Next Review Date: August 2012
Clinical Non-gyn Procedure nongyn21.01 Preparation Of Non-Gynecologic Specimens Final Approval: October 2010 Effective: October 2010 Next Review Date: August 2012 List all stakeholder(s) and dates of approval:
More informationIDENTIFYING UNKNOWN SUBSTANCES
IDENTIFYING UNKNOWN SUBSTANCES LAB 15 EXPERIMENT STUDENT BOOK Chapter 1, page 25 TOOLBOX Page 4 and 36 Goal Identify unknown substances with the help of different tests. 1. What is the independent variable
More informationHuman Myostatin, ELISA Kit (MSTN)
Human Myostatin, ELISA Kit (MSTN) 96 Tests Catalog Number: MBS733837 Store all reagents at 2-8 C Valid Period: six months For samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood
More informationNPTII ELISA Qualitative User Guide neomycin phosphotransferase II Catalog number: PSP 73000
List of contents Lot number Item 96 wells 288 wells 480 wells 4800 wells Antibody-coated 96-well microtiter plates 1 3 5 50 Peroxidase enzyme conjugate (bottles A and B) 0.150 ml 0.350 ml 0.550 ml 5.0
More informationAQUATITE. Also designed as: Crack/gap filler for masonry and unglazed ceramics Sealants against leaks Repairer for roof cracks and gutters
AQUATITE Revision Date: september 2007 Description Bondall AquaTite is a grey, brushable, waterproofing coating that forms a flexible yet tough, seamless membrane. This single pack product exceeds the
More informationOLD BRICK ORIGINALS KILN-FIRED THIN BRICK VENEER Installation Guide. RobinsonBrick.com
OLD BRICK ORIGINALS KILN-FIRED THIN BRICK VENEER Installation Guide RobinsonBrick.com OLD BRICK ORIGINALS THIN BRICK VENEER Page 2 INTRODUCTION Check local building codes for proper installation guidelines
More informationCranio-caudal Fixation Unit CC-320-Plus
Cranio-caudal Fixation Unit CC-320-Plus 118341 for immobilization combined with the NORAS Breast Biopsy 4-Ch Coil, the Siemens Breast Biopsy 4-Ch Coil, the Breast Biopsy 4-Ch Coil for GE or the NORAS Patient
More informationsuccessful sampling SAMPLING S Y S T E M S. Issue E.
successful sampling 2009-10 SAMPLING www.sampling.co.uk Issue E Sampling Systems SAMPLING Successful Sampling Starts Here Welcome to the new Sampling Systems catalogue. We hope that you will find the brochure
More informationProducts made in Switzerland
Products made in Switzerland HYGIENIC AND MEDICALLY CLEAR DIFFERENT PRODUCTS BUT THE SAME QUALITY LABEL HYGIENIC AND MEDICALLY CLEAR 2 MDD TECHNOLOGIES FOR: 1. Cleaning 2. Disinfection 3. High Level Disinfection
More informationDRAFT. Apollo 324 System. Protocol. PrepX-32i DNA Library
Apollo 324 System PrepX-32i DNA Library Protocol Copyright 2012, IntegenX Inc. All rights reserved. Information in this document is subject to change without notice. IntegenX assumes no responsibility
More informationBiomedical Waste Management Guide
Biomedical Waste Management Guide Yale University Office of Environmental Health & Safety 135 College Street, Suite 100, New Haven, CT 06510 Updated April 2017 Telephone: 203-785-3550 / Fax: 203-785-7588
More informationApplication of Avery Dennison SW 900 Series Films Instructional Bulletin #4.08 (Revision 2) Dated: 01/17/17
Application of Avery Dennison SW 900 Series Films #4.08 (Revision 2) Dated: 01/17/17 Contents 1.0 Overview 2.0 Benefits 3.0 Surface Preparation 4.0 Tools & Techniques 5.0 Surface Preparation 6.0 Application
More informationDEPARTMENT OF THE ARMY PYROTECHNICS BRANCH ABERDEEN PROVING GROUND, MARYLAND MEMORANDUM FOR Commander, Naval Sea System Command,
SMCCR-MUS-P DEPARTMENT OF THE ARMY PYROTECHNICS BRANCH ABERDEEN PROVING GROUND, MARYLAND 21020-5423 MEMORANDUM FOR Commander, Naval Sea System Command, SUBJECT: Report of Observations Nat Center Bldg.
More informationSouthern Blot Protocol with Digoxigenin (DIG) probe JAX Mice strain: C57BL/6J Tg(C9orf72_i3)112Lutzy/J
Southern Blot Protocol with Digoxigenin (DIG) probe JAX Mice strain: 023099 C57BL/6J Tg(C9orf72_i3)112Lutzy/J Tail Genomic DNA extraction with QIAGEN DNeasy Blood & Tissue Kit (QIAGEN, cat. no.69504 or
More informationAcetone is flammable; refer to the manufacturer s instructions for complete safety information.
Installing UPP FRP Entry Seals on Fiberglass Sumps Introduction This document is meant as an installation overview for UPP FRP entry seals with fiberglass sumps and should be used in conjunction with the
More informationThese conditions must be maintained during transport to the laboratory.
A successful laboratory analysis starts in the field. The result of an analysis is as good as the representativeness of the soil sample used. The sampling procedure is basically determined by the analyses
More informationDETERMINATION of the EMPIRICAL FORMULA
DETERMINATION of the EMPIRICAL FORMULA One of the fundamental statements of the atomic theory is that elements combine in simple whole number ratios. This observation gives support to the theory of atoms,
More informationPorcine IgM (Immunoglobulin M) ELISA Kit
Porcine IgM (Immunoglobulin M) ELISA Kit Catalogue No: EP0085 Size: 48T/96T Reactivity: Porcine Detection Range: 0.156-10ng/ml Sensitivity:
More informationCompetency Guide: Care and Handling of Rigid Endoscopes
Competency Guide: Care and Handling of Rigid Endoscopes This document details the basic steps to correctly process and maintain a rigid endoscope. This document can be used to perform a competency review
More informationPrinciples and Practice of Agarose Gel Electrophoresis
Edvo-Kit #101 Principles and Practice of Agarose Gel Electrophoresis Experiment Objective: The objective of this experiment is to develop a basic understanding of electrophoretic theory, and to gain "hands-on"
More informationQUALITY ASSURANCE IN AN MDRD
QUALITY ASSURANCE IN AN MDRD MAINTENANCE AND PREVENTATIVE MAINTENANCE MDRD is a machine dependent department washers, pasteurizers, cart washer, sterilizer, ultrasonic, heat sealers, RO water systems All
More informationEntry Level Assessment Blueprint Biotechnology
Entry Level Assessment Blueprint Biotechnology Test Code: 4075 / Version: 01 Specific Competencies and Skills Tested in this Assessment: Work Habits Demonstrate professional work habits Demonstrate the
More informationEH&S. Sheet. Fact. Safe and Effective Use of Autoclaves. What are autoclaves? Factors for effective sterilization. Dry heat cycle - when to use
Please post or circulate Fact heet nvironment, ealth and afety Information for the Berkeley Campus No. 33 Revised 04/04/11 afe and ffective Use of Autoclaves Autoclaves are easy to use but can pose a safety
More informationHuman placenta lactogen (HPL) ELISA Kit
Human placenta lactogen (HPL) ELISA Kit Catalog Number. CSB-E09665h For the quantitative determination of human placenta lactogen (HPL) concentrations in serum, plasma. This package insert must be read
More informationPreparation thin blood films and Giemsa staining
TLM_PRO_026 Version No.001 Page 1 of 5 Department: Clinical sciences Unit: Tropical Laboratory Medicine Preparation thin blood films and Giemsa staining Project/study: Not applicable 1. Scope and application
More informationNeural Stem Cells (ipsc from Blood Cells; Male)
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Neural Stem Cells (ipsc from Blood Cells; Male) Product Information Catalog Number ASE-9234 (Male) Description Applied StemCell's
More informationRepliform. Tissue Regeneration Matrix. Instructions for Use. Distributed by:
Repliform Tissue Regeneration Matrix Instructions for Use Distributed by: Boston Scientific Corporation 300 Boston Scientific Way Marlborough, MA 01752 Processed from Donated Human Tissue by: LifeCell
More informationStandard Operating Procedure
Standard Operating Procedure Section: Laboratory Version No. # 1.0 Initials: Revision Date: 20 April 2011 Title: 2.1B Processing of Blood Cultures_BacT/ALERT 1. Definitions 1.1. CRF- Case Report Form 2.
More informationBIOLOGY 163 LABORATORY. RESTRICTION MAPPING OF PLASMID DNA (Revised Fall 2017)
BIOLOGY 163 LABORATORY RESTRICTION MAPPING OF PLASMID DNA (Revised Fall 2017) Physical mapping of genomes is an important part of modern molecular genetics. As it's name implies, physical mapping seeks
More informationEmployee Environmental, Health & Safety Manual. INFECTIOUS WASTE MANAGEMENT PROGRAM Latest Revision: 05/27/08
Note: Print document as needed for MATC Departmental purposes; it is user responsibility to ensure use of latest revision by CONTENT checking online at: http://matcmadison.edu/ad/facilities/ehs/ under
More information2. Be sure to wear gloves and safety goggles, especially when using the disinfectant.
Mold Control INTRODUCTION In this activity, your class will test three different cleaners and then will determine which cleaner is most effective for controlling mold. You will analyze a series of dilutions
More informationSECTION SILOXANE WATER REPELLENTS
Eldorado Stone, LLC 1370 Grand Ave., Bldg., B San Marcos, CA 92069 Tel: (760) 736-3232 Fax: (760) 736-8890 e-mail: customerservice@eldoradostone.com website: www.eldoradostone.com SECTION 07 19 23 SILOXANE
More informationHuman Bordetella Pertussis IgG ELISA kit
Human Bordetella Pertussis IgG ELISA kit Catalog number: NR-R10157 (96 wells) The kit is designed to qualitatively detect Bordetella Pertussis IgG in Human serum or plasma. FOR RESEARCH USE ONLY. NOT FOR
More informationAdvanced FEA Stress Analysis of Medical Instruments and Devices using Femap, NX Nastran and LS-DYNA
Advanced FEA Stress Analysis of Medical Instruments and Devices Title: Medical device (aspiration tip) for bone marrow extraction for endoscopic surgery Keywords: Endoscopic surgical anvil, powder metallurgical
More informationHuman immunoglobulin G(IgG) ELISA Kit
Human immunoglobulin G(IgG) ELISA Kit Catalog Number. CSB-E07979h For the quantitative determination of human immunoglobulin G (IgG) concentrations in serum, plasma, cell culture supernates, urine, tissue
More informationMouse ALP (Alkaline Phosphatase) ELISA Kit
Mouse ALP (Alkaline Phosphatase) ELISA Kit Catalogue No.:MBS765655 Size:48T/96T Reactivity:Mouse Range:0.313-20ng/ml Sensitivity:
More informationDeciding how to handle waste produced from manufacturing
27 Reclaiming the Metal l a b o r at o ry Deciding how to handle waste produced from manufacturing is challenging. It can be diluted or incinerated, as you saw in previous activities. In some cases, it
More informationHealth Sciences Campus Biomedical Waste Management Standard Operating Procedure (SOP)
Health Sciences Campus Biomedical Waste Management Standard Operating Procedure (SOP) NOTE: This SOP for biological waste management does not supersede the requirements for radioactive and/or hazardous
More informationProbe Manual EL Rev D. Handling, cleaning and sterilization of the Medistim EL Imaging Probe
Handling, cleaning and sterilization of the Medistim EL 100015 Imaging Probe The purpose of this procedure is to guarantee that the probe performance is in accordance with the specifications during the
More informationCranio-caudal Fixation Unit CC-GE MR10063-CC-GE
Cranio-caudal Fixation Unit CC-GE MR10063-CC-GE Cranio caudal Fixation Unit CC-GE for Immobilization with the GE 8 channel breast coil Operator s Manual Revision 04 Issued Date: August 2016 Technichal
More information