Molecular characterization, detection & quantitation of biological products Purin Charoensuksai, PhD

Transcription

1 Molecular characterization, detection & quantitation of biological products Purin Charoensuksai, PhD Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University

2 Example of critical checkpoints influencing biological activity and safety of biological products Vector construction Expression Purification Storage until usage Coding sequence Modification of proteins PTMs Cleavages/truncation Aggregation Host cell proteins/ DNA Residual growth factors Adventitious agents Virus Micoplasma Stability of cell lines Location of insert Copy number of insert Ability to concentrate target species/clear up contaminants Leachables from columns Re-usage of columns Stability Storage conditions Picture in cover slide taken from: /biologics-world-taiwan-2016/the-concept/

3 Various laboratory techniques are routinely used for the characterization of biological products Nucleic acid AGE Hybridization Sequencing PCR, qpcr Microarray Protein Amino acid analysis Edman degradation Peptide mapping: HPLC, MS, MS-MS SDS-PAGE & protein staining IEF 2D-gel Immunological assays Precipitation/ agglutination Western blot ELISA Picture taken from:

4 Molecular assays for biological products Our main focuses: Brief review of the principle of each assay Example of their usage in biological product registration Multiple levels: nucleic acids, protein, immunological assays Common types of assays: Qualitative: detect the presence of something Quantitative: determine the exact (?) amount of something Limit test (semi-quantitative): check if the level of something exceeds certain amount

5 Molecular analysis of nucleic acids DNA agarose gel electrophoresis Nucleic acid hybridization DNA sequencing PCR, qpcr Microarray

6 DNA agarose gel electrophoresis (AGE) Separate fragments of DNA based on size 6

7 Porous structure of molecular sieve used to resolve DNA/proteins Picture taken from: ocw.mit.edu/courses/biological-engineering/ laboratory-fundamentals-in-biological-engineering-spring-2010/labs/module-1-day-2-purify-aptamer-encoding-dna/

8 DNA in agarose gels can be visualized by various staining methods Ethidium bromide Ethidium bromide Fluorescent DNA dye

9 Picture taken from Picture taken from:

10 Hybridization of nucleic acids Southern blot & Northern blot Southern blot > DNA Northern blot > RNA AGE 2. Transfer to solid support (blotting) 3. Hybridization with probe specific for sequence of interest 4. Detection Picture taken from: 10

11 Applications of nucleic acid hybridization DNA gel electrophoresis Southern Blotting TaqI & SmaI Ladder Uncut TaqI SmaI Ladder Uncut TaqI SmaI TaqI & SmaI Example: roughly map the location of DNA insert (infer genetic stability of cell line) Picture taken from:

12 DNA sequencing: Sanger method 12

13 Sanger sequencing is also known as a chain-termination method 13

14 DNA sequencing: Sanger method Chain-termination method 5 main components: DNA template Primer dntps fluorescent labelled ddntps DNA polymerase Sequence 1 fragment (read bps) at a time Example: check DNA sequence of insert (DNA or mrna) 14

15 Polymerase chain reaction (PCR) Amplification of target DNA 15

16 Quantitative Polymerase Chain Reaction (qpcr) Picture taken from:

17 Quantitative Polymerase Chain Reaction (qpcr) A B Picture taken from:

18 Some examples of PCR/qPCR applications for the characterization of biological products PCR Amplify desired gene from suitable host for expression Detection of adventitious agents e.g. virus or micoplasma Detection of host cell DNA qpcr Determine the copy number of insert in master cell, working cell or cell at the end of production

19 DNA microarray By Squidonius Based on hybridization between probes & DNA of interest A Large number of probes are fixed on a solid support (CHIP) enabling the interrogation of multiple targets simultaneously

20 Picture taken from: DNA microarray

21 Molecular analysis of proteins Polyacrylamide gel electrophoresis Edman degradation: N-terminal sequencing Peptide mapping: analysis of proteolytic cleavage pattern Mass-spectrometry: MS or MS-MS

22 Polyacrylamide gel electrophoresis (PAGE) Separate proteins based on size Usually performed in a denaturing condition (SDS-PAGE) Can be adapted to resolve proteins in their native conformation (native gel) 22

23 Proteins in polyacrylamide gels can be visualized by various staining methods Coomassie brilliant blue Silver stain Fluorescent stain e.g. Sypro Ruby

24 Edman degradation Phenyl isothiocyanate Phenylthiohydantoin (PTH)- amino acid derivatives are identified through chromatography N-terminal sequencing, up to 30 amino acids Will not work if N-terminal amino group is modified or buried within protein

25 Peptide mapping 1. Fragmentation of protein e.g. proteolytic cleavage by enzymes which cleave specific bond 2. Resolve peptides with appropriate methods e.g. SDS-PAGE, HPLC, MS, etc.

26 Peptide mapping Lys-C = K / X Arg-C = R /X

27 Some examples of peptide mapping applications in the registration of biological products Peptide mapping/finger printing reflects the identity of the parent protein Usage: Identity of drug substance Purity: detect modified forms of drug substance e.g. some PTMs

28 Protein mass-spectrometry (MS) analysis

29 2 types of protein mass-spectrometry (MS) analysis: Top-down and Bottom-up

30 Applications of protein MS analysis Mass fingerprint: MS Protein quantitation: quantitative MS Protein sequencing: MS-MS Post-tranlational modification characterization: MS-MS Example of MS application for the registration of biological products: analysis of amino acid variants e.g. deamidation, oxidation, glycation or glycosylation profile of drug substance

31 Immunological assays Exploit antigen-antibody interaction Examples: Precipitation/agglutination reactions Western blot ELISA

32 Precipitation/Agglutination

33 Precipitation/Agglutination Antibody/antigen interaction Similarity: antibody crosslink antigen and form precipitate Difference: nature of antigen Precipitation = soluble antigen Agglutination = insoluble antigen e.g. RBC, bacteria, antigen fixed on beads (HCG, bacterial toxins, etc.) Picture taken from:

34 Example of precipitation/agglutination reaction Hemaglutination assay

35 Western blot Resolve proteins by SDS-PAGE 35

36 Western blot 1. Transfer proteins in gel onto other solid membrane Nitrocellulose PVDF (Polyvinylidene difluoride) 2. Stain with antibody specific to the protein of interest 3. Detect with appropriate methods Colorimetric Chemiluminescent Fluorescent, IR etc. 36

37 Diversity of amino acid

38 Isoelectric focusing

39 2D-SDS-PAGE

40 ELISA Enzyme-Linked ImmunoSorbent Assay Comparison of test samples with standard antigen with known concentration yield semi-quantitative/quantitative measurement of antigen in test sample

41 Example of ELISA application for the registration of biological products Host cell protein Leachable protein A Antibiotics Insulin Some small molecules: HEPES, resin components, etc.

42 Various laboratory techniques are routinely used for the characterization of biological products Nucleic acid AGE Hybridization Sequencing PCR, qpcr Microarray Protein Amino acid analysis Edman degradation Peptide mapping: HPLC, MS, MS-MS SDS-PAGE & protein staining IEF 2D-gel Immunological assays Precipitation/ agglutination Western blot ELISA Picture taken from:

43 Thank you!