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1 Final Report: Germicidal Spray Test of ZeroMold Plus Page 1 of 18 FINAL REPORT STUDY TITLE Germicidal Spray Test of ZeroMold Plus Disinfectant SOUTHWEST RESEARCH INSTITUTE PROJECT NUMBERS (tebook# ) TESTING FACILITY Southwest Research Institute (SwRI ) Applied Physics Division Applied Power Division 6220 Culebra Road San Antonio, TX , USA Tel: , Fax: STUDY SPONSOR BIOSENTA Inc Finch Ave, West Suite 503 Toronto, Ontario, M3J 3H7, CANADA, PH: TEST GUIDELINE OCSPP TEST ORGANISMS: Staphylococcus aureus (ATCC 6538); Salmonella enterica (ATCC 10708); Listeria monocytogenes (ATCC 43257); Escherichia coli (ATCC 25922) TEST PRODUCT IDENTITY ZeroMold Plus TM (BI#0018-1, Lot #2, >60 days) ZeroMold Plus TM (BI# , Lot #3, Fresh lot) ZeroMold Plus TM (BI# , Lot #4, Fresh lot) STUDY DATES Date Sample Received: 02/17/2014 Study Initiation Date: 02/25/2014 Experimental Start Date: 3/03/2014 Experimental End Date: 05/02/2014 Study Completion Date: 05/02/2014

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5 Final Report: Germicidal Spray Test of ZeroMold Plus Page 5 of 18 TABLE OF CONTENTS TABL OF CONTENTS STUDY TITLE... 1 STATEMENT OF NO DATA CONFIDENTIALITY CLAIMS... 2 GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT... 3 TABL OF CONTENTS OBJECTIVE TEST SUBSTANCE, MATERIALS, EQUIPMENT & PERSONNEL... 6 Test Substance... 6 Materials and Equipment... 7 Personnel PROCEDURES PROTOCOL CHANGE AND STUDY DEVIATION Protocol Amendments Study Deviations: STUDY DESIGN CONTROLS AND ACCEPTANCE CRITERIA STUDY ACCEPTANCE CRITERIA CALCULATION AND STATISTICAL ANALYSIS RESULTS Staphylococcus aureus Salmonella enterica Listeria monocytogenes E. coli STUDY CONCLUSION REFERENCES APPENDICES... 18

6 Final Report: Germicidal Spray Test of ZeroMold Plus Page 6 of OBJECTIVE The purpose of this study is to determine the effectiveness of the Sponsor s product: ZeroMold Plus disinfectant, for hard surface disinfection following the Association of Analytical Communities (AOAC) Germicidal Spray Method in accordance with the Environmental Protection Agency (EPA) 40 CFR 160 Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) Good Laboratory Practice (GLP) Standards. This method is in compliance with the requirements of EPA (OCSPP ) and Health Canada Therapeutic Products Directorate (TPD). AOAC Official Method (2012) is applicable for testing spray and pressurized spray disinfectants to determine effectiveness as disinfectant for contaminated hard, nonporous, inanimate environmental surfaces. Tests to be conducted under this protocol include: GLP AOAC Germicidal Spray test (S. aureus; S. enterica) for Broad-Spectrum claim GLP AOAC Germicidal Spray test (Listeria) for specific Listeria claim GLP AOAC Germicidal Spray test (E. coli) for specific E. coli claim 2. TEST SUBSTANCE, MATERIALS, EQUIPMENT & PERSONNEL Test Substance Test substance information was shown below and active ingredient concentration was shown in Table 1. All ZeroMold Plus tested were previously titrated and diluted according to manufacturer s instructions to ensure comply with EPA guidance on LCL (SwRI GLP-SP-210 study). Name: ZeroMold Plus Active ingredient: NaClO Lower certified limit (LCL): 0.43% active ingredient concentration for efficacy testing: 0.43%-0.44 % (2% above LCL) Storage Conditions: Ambient Temperature, cap closed (all lots tested) Test substance spray condition is as follows: Distance = 6-8" Number of pumps = 4 Contact Time = 10 minutes Exposure Temperature = C (room temperature) Table 1. Test substance active ingredient concentration (LCL to up to 2% above LCL). ZeroMold Plus Active ingredient Concentration/Dilution Tested Test batch/lot information (NaClO) concentration BI#0018-1, Lot #2, >60 days 0.44% BI# , Lot #3, Fresh lot 0.43% Ready-to-use, Trigger Spray BI# , Lot #4, Fresh lot 0.44%

7 Final Report: Germicidal Spray Test of ZeroMold Plus Page 7 of 18 Materials and Equipment The materials and major equipment used in the study are indicated in Table 2 and 3. Table 2. Materials used for the project. Applicable COAs were archived in QA department. Materials CAS# Batch/Lot # Supplier Grade Expiry Ethanol Fisher Scientific Anhydrous, Oct/16 Histology Nutrient Broth N/A Becton Dickinson N/A 2016/02/29 Sodium Thiosulfate Sigma-Aldrich Anhydrous v/2018 Letheen Broth N/A Becton Dickinson N/A 2015/10/31 Phosphate Buffer N/A Remel/Fisher Scientific Butterfield 2014/11/27 Phosphate Buffer N/A Remel/Fisher Scientific Butterfield 2015/01/07 HyPure Water N/A AXE34730 HyClone Molecular May/2014 Biology Grade HyPure Water N/A AYF HyClone Molecular Jun/2015 Biology Grade Staphylococcus aureus N/A ATCC #6538 1/31/2015 Salmonella enterica N/A ATCC # /28/2016 Listeria N/A ATCC # /07 monocytogenes Escherichia coli N/A ATCC/Microbiologics # /04 Trypticase Soy Agar N/A Becton Dickinson Individual plates Trypticase Soy Agar Trypticase Soy Agar N/A Becton Dickinson Individual plates N/A Becton Dickinson Individual plates Hydrochloric acid SHBD5057V Sigma-Aldrich %, BioReagent Oct/2015 Sodium hydroxide SLBH8376V Sigma-Aldrich Reagent Oct/2015 grade, 98% pellets ph Buffer Kit Multiple, (see attached CoA) Fisher Scientific N/A Oct/14

8 Final Report: Germicidal Spray Test of ZeroMold Plus Page 8 of 18 Table 3. Major Equipment used in this study. Equipment Supplier /Model# Serial Number Timer Pipette Pipette Pipette Pipette Temp./RH Probe Thermometer Data Logger Fisher Scientific/S Eppendorf Research Plus, P200 Eppendorf Research Plus, P200 Eppendorf Research Plus, P1000 Socorex, Acura 815, P1000 Thermocouple Omega, type K -5 to +105 C Expiry if applicable Location /24/2015 B244, A 2/26/2015 B244, Z 2/26/2015 B244, Z 2/26/2015 B244, /26/2015 B244, Vaisala J /25/2014 B244, Omega, HH /18/2014 B244, Thermocouple Duro-Sense Corp., type K, -80 to +180 Incubator Lab-Line Imperial III, /18/2014 B244, 336 2/24/2015 B244, N/A B244, Autoclave Tuttnauer, 3870M N/A B244, Refrigerator /Freezer Refrigerator /Freezer Frigidaire, FRT17G4BW1 Fisher Scientific, Isotemp, A BA N/A B244, N/A B244, Calibration/ Verification documentation Verified and archived in QA department

9 Final Report: Germicidal Spray Test of ZeroMold Plus Page 9 of 18 Equipment Ultra Low -80C Freezer Scale Scale Check weights ph Meter Data Logging System Supplier /Model# Sanyo, MDF-C8V1 Sartorius, ED2202S Mettler Toledo AB104-S Serial Number Expiry if applicable Location N/A B244, /29/2014 B244, /07/2015 B244, Class N/S /13/2015 B244, Oakton ph/con 510 series N/A B244, N/A N/A N/A B244, Calibration/ Verification documentation Personnel The following persons contributed to the study: Xingguo Cheng, Ph.D., Senior Research Scientist, Study Director Spring Cabiness, M.S., Research Scientist Amy De los Santos, Research Scientist 3. PROCEDURES The following Test and Analytical Procedures (TAPs) were utilized in this study and are shown in Table 4. Table 4. TAPs used in this project and their applications (Appendix 1, archived by QA) TAP Name Number Autoclave Operation and Validation for use in the Microbiology Laboratory Use of the Oakton ph/con Meter in the Microbiology Laboratory Verification and Use of Automatic Pipettes in the Microbiology Lab Application Sterilization ph measurement Pipetting

10 Final Report: Germicidal Spray Test of ZeroMold Plus Page 10 of Calibration and Verification of Analytical Balances Weighing Calibration of Thermocouples and Relative Humidity Sensors Germicidal Spray Product: Disinfectant Testing Procedure Temperature Disinfectant testing 4. PROTOCOL CHANGE AND STUDY DEVIATION Protocol Amendments On 16 April 2014, amendment #1 was approved to change E. coli strain from ATCC 4157 to ATCC On 28 th April 2014, amendment #2 was approved to extend the study completion date to May 2 nd, Study Deviations: There are two study deviations (2014-NCR-0086 and 2014-NCR-0150) in this project: GLP protocol GLP-SP-214, TAP , Section Enumeration of viable bacteria from carriers, subsection Performed additional dilutions on the carrier counts to ensure the colony forming units per carrier for testing were captured. Performed 10-1 through 10-5 dilutions plated, whereas the protocol states dilutions 10-1 through 10-3 dilution should be plated. GLP protocol GLP-SP-214, TAP , Section 2, Table 1 and Section 7, Table 2. Client approved change in one of the test organisms from Escherichia coli (ATCC# 4157) to Escherichia coli (ATCC# 25922) an EPA reference control strain that is also used in susceptibility testing. The change of E. coli strain is acceptable since the previous bacteria strain cannot reach sufficient high density onto the carriers. The client agreed via and signed the GLP protocol amendment. 5. STUDY DESIGN The brief study design is shown in Figure 1 below. The detailed test procedure is shown in TAP in appendix 1. Brief test parameters are shown below: Type: Glass slides (25 mm 25 mm) Contact Time: 10 Minutes Test Temperature: C (room temperature) Growth Medium: Nutrient Media

11 Final Report: Germicidal Spray Test of ZeroMold Plus Page 11 of 18 Sub-culture Medium: (Modified) Letheen Broth Diluent Type: N/A Organic Soil Load: ne Dry Time: 30 minutes Dry Temperature: 36 ± 2 C Incubation Temperature: 36 ± 2 C Incubation Time: 48 ± 2 hours Test substance received at SwRI Preparation of s and Test organisms Inoculation of s (control and test) Exposure (Spray) of test substance onto carriers Test System Recovery after contact time Incubation and Observation for Study controls confirmation Figure 1. Schematic diagram showing the study design of this GLP study.

12 Final Report: Germicidal Spray Test of ZeroMold Plus Page 12 of CONTROLS AND ACCEPTANCE CRITERIA Purity A streak plate for isolation was performed on each organism culture and following incubation examined in order to confirm the presence of a pure culture. The acceptance criterion for this study control is a pure culture demonstrating colony morphology typical of the test organism. Sterility On the day of testing, a sterile, un-inoculated carrier will be placed into a tube of neutralizing subculture media. This subculture tube will be incubated as said test carrier tubes and examined for. The acceptance criterion for this study control is lack of to confirm the sterility of the carriers prior to test inoculation. Viability On the day of testing, two dried inoculated carriers will be placed into individual tubes of neutralizing subculture media. These subculture tubes will be incubated as said test carrier tubes and examined for. The acceptance criterion for this study control is which confirms exposure to and viability of the test organism. Neutralizing Subculture Medium Sterility A representative sample of un-inoculated neutralizing subculture medium will be incubated with said test carrier tubes and other controls and examined. The acceptance criterion for this study control is lack of which confirms sterility of prepared neutralizing subculture media. Neutralization Confirmation Effective neutralization of the test substance will be confirmed by exposing sterile carriers to the test substance and transferring them to subculture tubes containing the neutralizing media. The subculture tubes are inoculated with CFU of the test organism, incubated under test conditions and visually examined for the presence of. This control will be performed with multiple replicates using different dilutions of the test organism and performed for each test organism the test substance is tested against. A standardized spread plate will be run concurrently in order to enumerate the number of CFU actually added. The acceptance criterion for this study control is in the subculture tube, minimally, following inoculation with 100 CFU. population control (Enumeration of Viable Bacteria on ) Three inoculated carriers will be individually placed into a tube containing the subculture media. Each carrier tube will be vortexed to remove the bacteria from the carrier surface and then a series of 10-fold dilutions will be generated using phosphate-buffered dilution water. A sub-set of the dilution series will be plated, incubated, and the resulting colonies enumerated to determine the CFU per carrier. The log 10 density (LD) for each carrier will be determined and

13 Final Report: Germicidal Spray Test of ZeroMold Plus Page 13 of 18 used to calculate the average LD for the test. The mean LD acceptance criteria for this study control are organism dependent as shown in Table 5. Table 5. values of mean density, required per organism, when performing the Germicidal Spray Product test. Organism Average LD Corresponding Mean Density Staphylococcus aureus x 10 6 Salmonella enterica 4.0 to x x 10 5 Escherichia coli x 10 4 Listeria monocytogenes x STUDY ACCEPTANCE CRITERIA Test Substance Performance Criteria The U.S. EPA efficacy performance requirements for label claims of Broad-Spectrum Disinfectant requires 59 out of 60 carriers be negative for each batch tested and each organism. For Additional Bacteria label claims, 10 out of 10 carriers must be negative for of the test organism. Acceptance Criteria The study controls must perform according to the criteria detailed in the study controls description section above. If any of the control acceptance criteria are not met, the test may be repeated under the current protocol number. 8. CALCULATION AND STATISTICAL ANALYSIS Neutralization Inoculum Enumeration [(Plate Count 1 + Plate Count 2/2) / (dilution factor x plated volume)] = Neutralization control inoculum (CFU) Enumeration Population Calculation: CFU/ = ( average# ofcolonies / plate@ dilution) neutralizervolume ( volumeplated) x( dilutionfactor) Average Log 10 Population = Log10 X1+ Log10X 2 + Log10XN N

14 Final Report: Germicidal Spray Test of ZeroMold Plus Page 14 of 18 X= CFU/carrier N= number of control carriers 9. RESULTS Staphylococcus aureus and Neutralization Results and neutralization results are in compliance with the aforementioned study acceptance criteria. The neutralization control tube for S. aureus was inoculated with 39.5 CFU, which met the acceptance criteria. ZeroMold Plus BI# , Lot #4 BI# , Lot #3 BI#0018-1, Lot #2 Purity Sterility control Viability Neutralizing Subculture Medium Sterility Neutralization Confirmation population control SwRI test # Growth Growth 2.89 x Growth Growth 1.7 x Growth Growth 2.96 x Test Results ZeroMold Plus demonstrated the following results at a 10 minute contact time with no soil load. ZeroMold Plus at BioSenta Batch/Lot Test organism Contact time Exposure temperature* Soil load Number of s positive negative LCL number (ºC) carriers tested Fresh lot BI# min and , Lot #4 Fresh lot BI# , Lot #3 60 days BI#0018-1, old lot Lot #2 S. aureus # min and 21.2 ne min and * NOTE: There are 2 exposure temperatures for the tests, because the 60 carriers were processed in 2 groups of 30 to ensure all were able to be processed within 10 minute contact window

15 Final Report: Germicidal Spray Test of ZeroMold Plus Page 15 of 18 Results Salmonella enterica and Neutralization Results and neutralization results are in compliance with the aforementioned study acceptance criteria. The neutralization control tube for Salmonella enterica was inoculated with 44 CFU, which met the acceptance criteria. ZeroMold Plus BI# , Lot #4 BI# , Lot #3 BI#0018-1, Lot #2 Purity Sterility control Viability Neutralizing Subculture Medium Sterility Neutralization Confirmation population control SwRI test # Growth Growth 2.66 x Growth Growth 1.68 x Growth Growth 9.2 x Test Results ZeroMold Plus demonstrated the following results at a 10 minute contact time with no soil load. ZeroMold Plus at BioSenta Batch/Lot Test organism Contact time Exposure temperature* Soil load Number of s positive negative LCL number (ºC) carriers tested Fresh lot BI# min and 20.7 ne , Lot #4 Fresh lot BI# , Lot #3 60 days BI#0018-1, old lot Lot #2 Salmonella enterica # min and 20.4 ne min and 21.3 ne * NOTE: There are 2 exposure temperatures for the tests, because the 60 carriers were processed in 2 groups of 30 to ensure all were able to be processed within 10 minute contact window

16 Final Report: Germicidal Spray Test of ZeroMold Plus Page 16 of 18 Results Listeria monocytogenes and Neutralization Results and neutralization results are in compliance with the aforementioned study acceptance criteria. The neutralization control tube for Listeria monocytogenes was inoculated with 18 CFU, which met the acceptance criteria. ZeroMold Plus BI# , Lot #4 BI# , Lot #3 Purity Sterility control Viability Neutralizing Subculture Medium Sterility Neutralization Confirmation population control SwRI test # Growth Growth 3.9 x Growth Growth 3.77 x Test Results ZeroMold Plus demonstrated the following results at a 10 minute contact time with no soil load. ZeroMold BioSenta Test Contact Exposure Soil Number s Plus at Batch/Lot organism time temperature load of positive negative LCL number (ºC) carriers tested Fresh lot BI# Fresh lot 6, Lot #4 BI# , Lot #3 Listeria monocytogenes # min min ne ne

17 Final Report: Germicidal Spray Test of ZeroMold Plus Page 17 of 18 Results E. coli and Neutralization Results and neutralization results are in compliance with the aforementioned study acceptance criteria. The neutralization control tube for E. coli was inoculated with 56 CFU, which met the acceptance criteria. ZeroMold Plus BI# , Lot #4 BI# , Lot #3 Purity Sterility control Viability Neutralizing Subculture Medium Sterility Neutralization Confirmation population control SwRI test # Growth Growth 1.8 x Growth Growth 1.27 x Test Results ZeroMold Plus demonstrated the following results at a 10 minute contact time with no soil load. ZeroMold BioSenta Test Contact Exposure Soil Number s Plus at Batch/Lot organism time temperature load of positive LCL number (ºC) carriers tested Fresh lot BI# , Lot #4 Fresh lot BI# , Lot #3 E. coli # min min ne ne negative 10. STUDY CONCLUSION In conclusion, our GLP testing results below indicated that ZeroMold Plus at LCL passed all the microorganisms tested: Staphylococcus aureus (#6538), Salmonella enterica (#10708), Listeria monocytogenes (ATCC 43257); Escherichia coli (ATCC 25922). ZeroMold Plus at LCL using the three batches tested met the product performance guidelines for hard surface disinfectants.

18 Final Report: Germicidal Spray Test of ZeroMold Plus Page 18 of RECORD AND SAMPLE RETENTION The study for a research or marketing permit approved by EPA shall be maintained for the period during which the sponsor holds that research or marketing permit to which the study is pertinent unless all originals are provided to the client. For samples remaining at the conclusion of the study SwRI will either have samples returned to the sponsor or dispose the sample after five years from market permit submission to EPA. Materials that degrade will not be maintained. REFERENCES U.S. Environmental Protection Agency, Office of Chemical Safety and Pollution Prevention, Product Performance Test Guidelines, OCSPP : General Considerations for Public Health Uses of Antimicrobial Agents, March 12, EPA Product Performance Test Guidelines; OCSPP : Disinfectants for Use on Hard Surfaces-Efficacy Data Recommendations AOAC Official Method Germicidal Spray Products as Disinfectants (2012) US Environmental Protection Agency, Office of Pesticide Programs, Lower Certified Limit Testing Guidance, APPENDICES Appendix 1. TAPs (summarized in Table 4) used in this study.

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