Protein Synthesis. DNA to RNA to Protein
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1 Protein Synthesis DNA to RNA to Protein
2 From Genes to Proteins Processing the information contained in DNA into proteins involves a sequence of events known as gene expression and results in protein synthesis. Proteins are not built directly from genes; hereditary information is transferred from DNA to a working set of directions - RNA
3 From Genes to Proteins Gene expression occurs in two steps: transcription translation
4 RNA In eukaryotes, the genes directing protein production are in the nucleus and the enzymes and amino acid building blocks for proteins are found at the ribosomes in the cytoplasm. Before proteins are made from these genes, an RNA molecule must be made RNA, ribonucleic acid, is responsible for taking the genetic information from the DNA in the nucleus to the site of protein synthesis.
5 Structure of RNA RNA differs from DNA in three main ways: RNA is single stranded RNA contains ribose not deoxyribose RNA contains uracil (U) not thymine (bonds with adenine)
6 Types of RNA Three types of RNA are essential for gene expression: mrna - carries information from DNA to ribosomes (working copy of DNA) trna - carries amino acids to ribosomes rrna - plays a structural role in ribosomes
7 Overview: Cell assembles a molecule of RNA using DNA as a template. In eukaryotic organisms this takes place in the nucleus Steps: Transcription begins when RNA polymerase binds to a region of DNA called a promoter - a start signal on the DNA Transcription
8 Steps of Transcription DNA unwinds and unzips - exposing nitrogen bases; one strand serves as template RNA polymerase moves down strand, reads each nucleotide and pairs it with a complementary RNA base single stranded RNA 60 nucleotides/sec RNA polymerase continues until it reaches a stop signal - terminator DNA rezips after polymerase passes by
9 Transcription
10 Protein Synthesis Proteins are made up of one or more polypeptides, each of which consists of a specific sequence of amino acids linked together by peptide bonds. There are 20 types of amino acids that make up proteins. The sequence of these amino acids (originally determined in the DNA code) determines the structure and function of the proteins.
11 Genetic code All organisms have a genetic code made of three-nucleotide sequences called codons. The codon is located on the mrna. Each of the 64 mrna codons corresponds to a specific amino acid, a start or a stop signal The genetic code is nearly universal. Sequences code for the same amino acids in different organisms
12 Genetic code
13 Translation Overview: Cell produces a protein Occurs at the ribosome in the cytoplasm where the cell keeps its supply of trna Tools of translation (in addition to mrna) trna Ribosomes (rrna)
14 trna trna is a single stranded RNA molecule that is folded into a cloverleaf One of the loops has a three nucleotide sequence called an anticodon which complements the mrna codon Opposite the anticodon is a site for attachment of a.a. which corresponds to a particular codon
15 Ribosomes Ribosome are composed of rrna and proteins. Ribosomes have three binding sites: one site to hold mrna with open codon A site - holds trna molecule carrying a.a. P site - also holds t RNA and attaches a.a. to growing protein chain
16 Translation: Begins when mrna binds to the smaller ribosomal subunit. Large subunit attaches (ribosome is complete). A start codon on mrna (usually AUG) signals beginning of protein chain trna molecule with anticodon UAC and methionine attached will bind to start codon in P site
17 Translation continued Codon in the vacant A site accepts the next trna Both P site and A site are now holding trnas with attached a.a. Peptide bond forms between the two a.a.
18 Translation continued The trna in the P site detaches and moves away The trna in the A site moves into the P site The new codon in the A site attracts the complementary trna with a.a.
19 Translation continued The sequence continues until a stop codon (for which there is no anticodon)is reached With nothing left to fit empty A site, ribosome complex falls apart Newly made protein is released into cell
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