VALIDATION GUIDE FOR CHROMOGENIC ASSAY FOR ACTIZYME Anti-FIIa

Size: px
Start display at page:

Download "VALIDATION GUIDE FOR CHROMOGENIC ASSAY FOR ACTIZYME Anti-FIIa"

Transcription

1 VALIDATION GUIDE FOR CHROMOGENIC ASSAY FOR ACTIZYME Anti-FIIa Chromogenic assay for testing heparins (UFH, LMWH and Enoxaparin) in purified systems by measurement of FIIa Inhibition, in compliance with pharmacopoeias (EP/BP) and FDA guidelines. Document History : CodificatIon History Date Version#1.0 VALIDATION DATA OF Heparin (anti-fiia) Cat No # KBBA Approved Quality Approved Approved Operations Control Product Development Head Mihir Parmar Trupti B. Kalpesh Jain

2 Table Contents : 1 Introduction 2 Experimental procedures 2.1 Reagents 2.2 Material to be provided by the End-User 2.3 Storage and Stability Information 2.4 Health Hazard Warning 2.5 Assay Protocol 2.6 Statistical analysis 2.7 Validation of the anti-factor IIa method Linearity Precision Accuracy 3 Result and Discussion 4 Conclusion 1. Introduction: Heparin (anti F-IIa) is a chromogenic assay intended for the quantitative determination of therapeutic heparin in purified solutions by measurement of factor IIa (thrombin) activity. The kit can be used for 200 test reactions as per microtiter plate protocol and 100 test reactions as per test tube protocol. The inhibitory effect of anti-thrombin III (AT-III) on factor IIa (thrombin), factor Xa and other coagulation serine proteases in plasma is increased several thousand-fold by heparin. This inhibition accounts for the anticoagulant effect of heparin. The quantitative determination of heparin levels by the measurement of their anti-factor IIa activity is a necessary tool for monitoring treatment efficacy. Low molecular weight heparin (LMWH) preparations appear to catalyze the reaction between thrombin (factor IIa) and AT-III less readily than the reaction between factor Xa and AT-III. Unfractionated heparin (UFH) catalyzes both reactions equally. In the assay, the rate of factor IIa inhibition is directly proportional to the heparin concentration since both factor IIa and AT-III are in excess. The residual factor IIa activity is inversely proportional to the heparin. 2. Experimental Procedure: 2.1. Reagents 1. Human Thrombin Reagent 2. Human Anti-thrombin III Reagent 3. Chromogenic Substrate 4. Instruction Manual 2.2 Materials to be provided by the End-User: 1. Microplate Reader / Spectrophotometer able to measure absorbance at 405nm 2. Adjustable pipettes to measure volumes ranging from 25μl to 2500μl, duly calibrated 3. Deionized (DI) water 4. Parallel line software for data analysis 5. Plastic tubes or cuvettes or microtiter plates with overflow capacity 350µl/well C water bath or microplate incubator preferred (for microplate method) 7. Timer/Stop watch 8. Glacial Acetic Acid 9. Absorbent paper 10. Tris (hydroxymethyl) aminomethane 11. Sodium Chloride 12. EDTA 13. Bovine Serum Albumin (BSA) 2.3 Storage and Stability Information: Un-reconstituted reagents are stable until the expiration date indicated on the label when stored at 2 to 8 C.

3 1. Human Thrombin Reagent: Reconstituted reagent is stable for 2 weeks at 2 to 8 C and for 4 months at -20 C. 2. Human Anti-thrombin III Reagent: Reconstituted reagent is stable for 2 weeks at 2 to 8 C and for 4 months at -20 C. 3. Chromogenic Substrate: Reconstituted reagent is stable for 2 weeks at 2 to 8 C and for 4 months at -20 C. 4 Dilution Buffer and Acetic acid are to be freshly prepared, prior to use. 2.4 Health Hazard Warnings: 1. The source material for the human anti-thrombin III has been found to be non-reactive for Hepatitis B Surface Antigen (HBsAg), Hepatitis C Virus (HCV) and Human Immunodeficiency Virus Type 1 and Type 2 (HIV-1, HIV-2) using FDA approved methods. 2. The Heparin (anti-fiia) antithromin III reagent contains sodium azide that may react with lead or copper plumbing to form highly explosive azides. Specimen Collection and Handling: Purified Samples: Dilute the heparin preparation with Dilution Buffer in order to bring it at a concentration within the assay working range. Reagent Preparation (all reagents should be diluted immediately prior to use): 1. Human Thrombin Reagent: Human Thrombin Reagent is a lyophilized preparation. Allow reagent to equilibrate to room temperature. Reconstitute with 5 ml of deionized water. Gently mix and leave to stand for 15 mins. 2. Human Anti-thrombin III Reagent: Anti-thrombin III is a lyophilized preparation. Allow reagent to equilibrate to room temperature. Reconstitute with 5 ml of deionized water. Gently mix and leave to stand for 15 mins. 3. Chromogenic Substrate: Chromogenic Substrate is a lyophilized preparation. Allow reagent to equilibrate to room temperature. Reconstitute with 5 ml of deionized water. Gently mix and leave to stand for 15 mins. 4. Dilution Buffer: (Not provided in the kit) to be prepared separately as follows: Dissolve gm of Sodium Chloride, 6.10 gm of tris (hydoxymethyl) amino methane, 2.80 gm of EDTA and 2.0 gm of bovine albumin in 800 ml of water. Adjust the ph 7.4 with hydrochloric acid and dilute to 1000 ml with water. 5. Acetic Acid Solution: (Not provided in the kit) to be prepared separately as follows: Dilute Glacial Acetic Acid with Distilled Water or Deionized Water in the ratio of 42: Assay Protocol Two step protocol for measurement of factor IIa activity in purified systems, in compliance with Pharmacopoeias (EP/ BP) and FDA guidelines. Note: 1. The Standard Dilution Samples (S1, S2, S3, and S4) and Test Dilution Samples (T1, T2, T3, and T4) have to be diluted in same ratios to the nearest third decimal concentrations to assure high accuracy and estimation by extrapolation. 2. The dilution steps prior to the Standard and Test Dilution also should be to the nearest concentration possible to assure high accuracy. 3. Please see an example of the dilution steps followed to get a high degree of accuracy when assaying the Test Samples. 4. Dilute Standard and Test Samples with the Dilution buffer ph 7.4, mentioned in Reagent Preparation. For Example: Preparation of Standard Stock Standard Concentration IU/mg is to be diluted to make Stock Solution strength of IU/ml

4 Standard Dilution Test Sample Dilution Stock Solution strength: IU/ml ml 2 ml IU/ml 0.1 ml 2 ml 5 IU/ml 1.5 ml 2 ml IU/ml 0.2 ml 2 ml 0.5 IU/ml 0.8 ml 2 ml IU/ml ml 2 ml IU/ml 1.5 ml 2 ml IU/ml 0.8 ml 2 ml IU/ml S ml 2 ml IU/ml T ml 2 ml IU/ml S ml 2 ml IU/ml T ml 2 ml IU/ml S ml 2 ml IU/ml T ml 2 ml IU/ml S ml 2 ml IU/ml T ml 2 ml IU/ml Add the reagents into the microwell or plastic test tube, as per following steps: microwell test tube Standard or Test Sample 25µl 50µl Anti-thrombin III 100µl 125µl Mix but do not allow bubbles to form. Incubate at 37⁰C, for 1 minute Human Thrombin Reagent 50µl 100µl Mix and incubate at 37⁰C, for exactly 1 minute Chromogenic Substrate 50µl 175µl Mix and incubate at 37⁰C, for 4 minutes Acetic Acid 125µl 250µl Mix and measure the absorbance at 405nm 2.6. Statistical analysis Statistical analysis of the assay data were carried out according to PLAv28 (Excel based worksheet/software based on DJ Finney, Statistical Method in Biological Assay, 3rd edition by parallel line methods). Analysis of variance was performed for each independent, assay and the assumption of linearity and parallelism of the log dose log response lines was tested. 2.7 Validation of the anti-factor IIa method In this procedure we followed the microwell method and the parameters were evaluated according to the EP/USP guidelines Linearity The Standard of low molecular weight heparins specifying the activity as IIa/ vial, was reconstituted with 5 ml of distilled water. Appropriate amounts of this stock solution were diluted in Tris buffer (0.05 M, ph 7.4) yielding concentrations of , , , , , , and IU/ml. Two independent calibration curves were constructed and the linearity evaluated by linear regression analysis, which was calculated by the least square regression method Precision The precision of the method was determined by repeatability and intermediate precision and was expressed as the relative standard deviation. The repeatability was examined by assaying one samples of Heparin on the same day (intra-day) and under the same experimental conditions, against Standard of low molecular weight heparins. The intermediate precision of the method was evaluated through the performance of the analysis on two different days (inter-days) and by having another analyst performing the analysis in the same laboratory (between-analysts).

5 ACTIZYME Anti-FIIa Intra Assay Run#1 Run#2 Run#3 Run#4 %C.V Sample Concentration (IU/ml) Standard Concentration (IU/ml) Inter Assay Run#1 Run#2 %C.V Sample Concentration IU/ml Standard Concentration IU/ml Accuracy To determine the accuracy of the proposed method, the test was performed over 4 concentration levels covering the specified range, and carrying out assay; each one in duplicate with the results being combined statistically. ACTIZYME Anti-FIIa Intra Assay Inter Assay Standard Standard Concentration Run#1 Run#2 Run#3 Run#4 % CV Concentration Run#1 Run#2 % CV Sample Run#1 Run#2 Run#3 4 % CV Sample Run#1 Run#2 % CV Concentration Concentration Results and Discussion: For each series, we calculate the regression of the absorbance against log concentration of the sample solutions and the standard solutions. The four independent log relative potency estimates are then combined to obtain the final geometric mean. Its confidence limits are calculated. Standard and Test Samples being serial diluted should pass the test for linearity and parallelism as the interpretation is done by extrapolating the data. The kit indicates a precision of <=1% and an accuracy of <=1%. The confidence limits were set at +/- 10%. 4. Conclusion: The Anti-Factor IIa assay was validated and the kit is accurate and complies with the parameters for bioassays as per EP/USP guidelines.

Mouse Factor XII Total ELISA Kit

Mouse Factor XII Total ELISA Kit Mouse Factor XII Total ELISA Kit Catalog No: IMFXIIKT-TOT Lot No: SAMPLE INTENDED USE This mouse coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII

More information

Human IgG Antigen ELISA Kit

Human IgG Antigen ELISA Kit Human IgG Antigen ELISA Kit Catalog No: IHUIGGKT Lot No: SAMPLE INTENDED USE This human immunoglobulin G antigen assay is intended for the quantitative determination of total human IgG antigen in serum,

More information

AssayMax Human Antithrombin III ELISA Kit

AssayMax Human Antithrombin III ELISA Kit AssayMax Human Antithrombin III ELISA Kit Catalog # EA3302-1 Introduction The serine-protease-inhibitor antithrombin III (AT III), the most important natural inhibitor of thrombin activity, has been shown

More information

Human Myostatin, ELISA Kit (MSTN)

Human Myostatin, ELISA Kit (MSTN) Human Myostatin, ELISA Kit (MSTN) 96 Tests Catalog Number: MBS733837 Store all reagents at 2-8 C Valid Period: six months For samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood

More information

Human PAI-1 Activity ELISA Kit

Human PAI-1 Activity ELISA Kit Human PAI-1 Activity ELISA Kit Catalog No: IHPAIKT Lot No: SAMPLE INTENDED USE This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1)

More information

Human IgM Ready-SET-Go!

Human IgM Ready-SET-Go! PRODUCT INFORMATION & MANUAL Human IgM Ready-SET-Go! 88-50620 Ready-SET-Go! Enzyme-linked Immunosorbent Assay for quantitative detection of human IgM. For research use only. Human IgM Ready-SET-Go! ELISA

More information

Human alpha-1-antitrypsin ELISA Kit

Human alpha-1-antitrypsin ELISA Kit AssayMax Human alpha-1-antitrypsin ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or

More information

ImmuLux Human IL-6 Fluorescent ELISA Kit

ImmuLux Human IL-6 Fluorescent ELISA Kit INSTRUCTIONS ImmuLux Human IL- Fluorescent ELISA Kit 3747 N. Meridian Road P.O. Box 7 Rockford, IL 05 840 53w Product Description Number Description 840 ImmuLux Human IL- Fluorescent ELISA Kit The Pierce

More information

Human Protein Z ELISA Kit

Human Protein Z ELISA Kit AssayMax Human Protein Z ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Dopamine Research ELISA

Dopamine Research ELISA v Instructions for use Research ELISA BA E-5300 Research ELISA 1. Intended use and principle of the test Enzyme Immunoassay for the quantitative determination of. Flexible test system for various biological

More information

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests

Human IgG ELISA Kit. Strip well format. Reagents for up to 96 tests Human IgG ELISA Kit Strip well format. Reagents for up to 96 tests Catalog No. CS222A Quantity: 1 x 96 tests CS222B 5 x 96 tests Intended Use: Background: Assay Principle: This human immunoglobulin G antigen

More information

Human Alpha-2-Macroglobulin ELISA

Human Alpha-2-Macroglobulin ELISA Human Alpha-2-Macroglobulin ELISA Catalog Number M046034 For the quantitative determination of alpha-2-macroglobulin in human serum and plasma samples. For research use only. This product insert must be

More information

AssayMax Human Albumin ELISA Kit

AssayMax Human Albumin ELISA Kit AssayMax Human Albumin ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Human Plasmin Activity Assay ELISA Kit

Human Plasmin Activity Assay ELISA Kit Human Plasmin Activity Assay ELISA Kit Catalog No. CSI12527A 1 x 96 tests CSI12527B 5 x 96 tests Intended Use: Background: Assay Principle: Reagents Provided: This human plasmin activity assay is for the

More information

Mouse Collagen Type III ELISA

Mouse Collagen Type III ELISA Mouse Collagen Type III ELISA Catalog Number M046062 For the quantitative determination of Collagen Type III in mouse plasma and serum samples. For research use only. This product insert must be read in

More information

Human Angiotensin 2 (Ang2) ELISA

Human Angiotensin 2 (Ang2) ELISA Human Angiotensin 2 (Ang2) ELISA For the quantitative determination of human Ang2 in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-52748 For Research Use Only. Not for use in

More information

Human IL-10 ELISA MAX Set Deluxe

Human IL-10 ELISA MAX Set Deluxe Human IL-10 ELISA MAX Set Deluxe Cat. No. 430604 (5 plates) 430605 (10 plates) 430606 (20 plates) ELISA Set for Accurate Cytokine Quantification from Cell Culture Supernatant, Serum, Plasma or Other Body

More information

AssayMax Human Factor XIII ELISA Kit

AssayMax Human Factor XIII ELISA Kit AssayMax Human Factor XIII ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Mouse Gonadotropin Releasing Hormone (GnRH) ELISA

Mouse Gonadotropin Releasing Hormone (GnRH) ELISA KAMIYA BIOMEDICAL COMPANY Mouse Gonadotropin Releasing Hormone (GnRH) ELISA For the quantitative determination of mouse GnRH in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-58182

More information

Human IL-1 alpha ELISA Kit

Human IL-1 alpha ELISA Kit Human IL-1 alpha ELISA Kit Catalog No: CDK025B Quantity: 2 x 96 tests SPECIFICITY : RANGE : SENSITIVITY : INCUBATION : SAMPLE TYPES : Recognizes both natural and recombinant human IL-1 alpha 31.2 pg /

More information

Human Haptoglobin ELISA Quantitation Kit. Manual

Human Haptoglobin ELISA Quantitation Kit. Manual Human Haptoglobin ELISA Quantitation Kit Manual Catalog number: 40-288-20080F For the quantitative determination of human Haptoglobin levels in serum or other biological samples GenWay Biotech, Inc. 6777

More information

Human IL-6 ELISA Set

Human IL-6 ELISA Set Human IL-6 ELISA Set Catalog No. CDK082B Quantity: 10 x 96 tests PRODUCT SPECIFICATIONS : Specificity: Recognizes both natural and recombinant human IL-6 Range: 6.25 pg / ml - 200 pg / ml Sensitivity:

More information

Human Calprotectin ELISA Kit

Human Calprotectin ELISA Kit AssayMax Human Calprotectin ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Human IgG Rubella ELISA Kit

Human IgG Rubella ELISA Kit Human IgG Rubella ELISA Kit Catalog No: IRAPKT2020 Lot No: SAMPLE INTENDED USE The Rubella IgG ELISA is intended for use in evaluating a patient s serologic status to the rubella virus infection. It is

More information

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT PAGE 1 HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN CTGF CONCENTRATIONS IN SERUM AND PLASMA ALWAYS REFER TO LOT SPECIFIC PROTCOL PROVIDED WITH EACH

More information

Mouse Peptide YY (PYY) ELISA

Mouse Peptide YY (PYY) ELISA Mouse Peptide YY (PYY) ELISA For the quantitative determination of mouse PYY in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-58705 For Research Use Only. Not for use in diagnostic

More information

Human Prekallikrein ELISA Kit

Human Prekallikrein ELISA Kit AssayMax Human Prekallikrein ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

IgG Antibodies TO Rubella Virus ELISA Kit Protocol

IgG Antibodies TO Rubella Virus ELISA Kit Protocol IgG Antibodies TO Rubella Virus ELISA Kit Protocol (Cat. No.:EK-310-81) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED

More information

Fibrinogen ELISA. For the quantitative determination of fibrinogen in biological fluids, serum, and plasma.

Fibrinogen ELISA. For the quantitative determination of fibrinogen in biological fluids, serum, and plasma. Fibrinogen ELISA For the quantitative determination of fibrinogen in biological fluids, serum, and plasma. Please read carefully due to Critical Changes, e.g., Changes to preparation of standard volume

More information

Human vascular endothelial cell growth factor A (VEGF-A) ELISA Kit

Human vascular endothelial cell growth factor A (VEGF-A) ELISA Kit Human vascular endothelial cell growth factor A (VEGF-A) ELISA Kit For the quantitative determination of human vascular endothelial cell growth factor A (VEGF-A) concentrations in serum, plasma, tissue

More information

Human Factor XII ELISA Kit

Human Factor XII ELISA Kit Human Factor XII ELISA Kit Assaypro LLC 30 Triad South Drive St. Charles, MO 63304 T (636) 447-9175 F (636) 447-9475 www.assaypro.com For any questions regarding troubleshooting or performing the assay,

More information

IgG Antibodies To Toxoplasma. Gondii ELISA Kit

IgG Antibodies To Toxoplasma. Gondii ELISA Kit IgG Antibodies To Toxoplasma Catalog No: IRAPKT1405 Gondii ELISA Kit Lot No: SAMPLE INTENDED USE The Toxoplasma IgG ELISA is intended for use in evaluating a patient s serologic status to Toxoplasma gondii

More information

Bovine prolactin/luteotropic hormone (PRL/LTH) ELISA Kit

Bovine prolactin/luteotropic hormone (PRL/LTH) ELISA Kit Bovine prolactin/luteotropic hormone (PRL/LTH) ELISA Kit Catalog Number. MBS703224 For the quantitative determination of bovine prolactin/luteotropic hormone (PRL/LTH) concentrations in serum, plasma.

More information

ab46070 TNF alpha Rat ELISA Kit

ab46070 TNF alpha Rat ELISA Kit Version 5 Last updated 29 September 2017 ab46070 TNF alpha Rat ELISA Kit For the quantitative measurement of Tumor Necrosis Factor alpha (TNF alpha) in rat cell culture supernatants, serum and plasma samples.

More information

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT PAGE 1 HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN CTGF CONCENTRATIONS IN SERUM AND PLASMA PURCHASE INFORMATION: ELISA NAME Catalog No. Lot No. Formulation

More information

AssayMax Human Apo-B ELISA Kit

AssayMax Human Apo-B ELISA Kit AssayMax Human Apo-B ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing the

More information

Mouse Luteinizing Hormone (LH) ELISA

Mouse Luteinizing Hormone (LH) ELISA Mouse Luteinizing Hormone (LH) ELISA For the quantitative determination of mouse LH in serum, plasma and tissue homogenates Cat. No. KU-222 For Research Use Only. Not for use in diagnostic procedures.

More information

Human placenta lactogen (HPL) ELISA Kit

Human placenta lactogen (HPL) ELISA Kit Human placenta lactogen (HPL) ELISA Kit Catalog Number. CSB-E09665h For the quantitative determination of human placenta lactogen (HPL) concentrations in serum, plasma. This package insert must be read

More information

****** Competition ELISA Kit Instruction

****** Competition ELISA Kit Instruction FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC PURPOSES ****** Competition ELISA Kit Instruction Kit name and catalog number Your analyte ELISA Kit, Catalog#: ***** Intended use The kit is used to detect the

More information

Human cross linked N-telopeptide of type I collagen (NTX) ELISA Kit

Human cross linked N-telopeptide of type I collagen (NTX) ELISA Kit Human cross linked N-telopeptide of type I collagen (NTX) ELISA Kit Cat.No: DEIA4005 Lot. No. (See product label) Size 96T Intended use For the quantitative determination of human cross linked N-telopeptide

More information

Human Factor IX (FIX)

Human Factor IX (FIX) Human Factor IX (FIX) ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO USA 63301 T: +1 (636) 447-9175 F: +1 (636) 395-7419 www.assaypro.com Emergo Europe Molenstraat 15 2513 BH The Hague

More information

Human Corticosterone ELISA Kit

Human Corticosterone ELISA Kit Human Corticosterone ELISA Kit Catalog No: IRAPKT3002 Lot No: SAMPLE INTRODUCTION Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid

More information

Human IL-6 ELISA. For the quantitative determination of IL-6 in human cell culture supernates, serum and plasma (heparin, EDTA, citrate)

Human IL-6 ELISA. For the quantitative determination of IL-6 in human cell culture supernates, serum and plasma (heparin, EDTA, citrate) K-ASSAY Human IL-6 ELISA For the quantitative determination of IL-6 in human cell culture supernates, serum and plasma (heparin, EDTA, citrate) Cat. No. KT-1348 For Research Use Only. Not for diagnostic

More information

Human Amyloid Beta Peptide 1-42 (Aβ1-42) ELISA Kit

Human Amyloid Beta Peptide 1-42 (Aβ1-42) ELISA Kit Human Amyloid Beta Peptide 1-42 (Aβ1-42) ELISA Kit Catalog Number. For the quantitative determination of human amyloid beta peptide 1-42 (Aβ1-42) concentrations in serum, plasma, tissue homogenates, cerebrospinal

More information

AssayMax TM. Human IgG ELISA Kit. Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO T (636) F (636)

AssayMax TM. Human IgG ELISA Kit. Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO T (636) F (636) AssayMax TM Human IgG ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

AssayMax Human IL-6 ELISA Kit

AssayMax Human IL-6 ELISA Kit AssayMax Human IL-6 ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing the

More information

Herpes Simplex Virus Type 1 (HSV-1) IgM ELISA Kit Protocol

Herpes Simplex Virus Type 1 (HSV-1) IgM ELISA Kit Protocol Herpes Simplex Virus Type 1 (HSV-1) IgM ELISA Kit Protocol (Cat. No.:EK-310-93) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com

More information

Product datasheet. Storage recommendations Store the kit at 2-8 C. The kit is stable for a period of up to 3 months from the date of receipt.

Product datasheet. Storage recommendations Store the kit at 2-8 C. The kit is stable for a period of up to 3 months from the date of receipt. Product datasheet Human VEGF-A ELISA Kit Product #: 0028 Storage recommendations Store the kit at 2-8 C. The kit is stable for a period of up to 3 months from the date of receipt. Description This human

More information

Human Collagen Type III (COL3) ELISA

Human Collagen Type III (COL3) ELISA Human Collagen Type III (COL3) ELISA For the quantitative determination of human COL3 in serum, plasma, cell culture fluid and other biological fluids Cat. No. KT-61018 For Research Use Only. Not for use

More information

AssayMax Human Factor XI ELISA Kit

AssayMax Human Factor XI ELISA Kit AssayMax Human Factor XI ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT HUMAN CTGF ELISA KIT PAGE 1 HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT PURCHASE INFORMATION: ELISA NAME HUMAN CTGF ELISA FOR THE QUANTITATIVE DETERMINATION OF HUMAN CTGF CONCENTRATIONS IN SERUM

More information

Human alpha-1-acid Glycoprotein ELISA Kit

Human alpha-1-acid Glycoprotein ELISA Kit AssayMax TM Human alpha-1-acid Glycoprotein ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting

More information

Cyfra 21-1 IRMA. Product information Information about other products is available at: Userś Manual DE52100

Cyfra 21-1 IRMA. Product information Information about other products is available at:  Userś Manual DE52100 Product information Information about other products is available at: www.demeditec.com Userś Manual Cyfra 21-1 IRMA The CYFRA 21.1 IRMA system provides a direct in vitro quantitative determination of

More information

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT

HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT HUMAN CTGF ELISA KIT Page 1 HUMAN CONNECTIVE TISSUE GROWTH FACTOR (CTGF) ELISA KIT PURCHASE INFORMATION: FOR THE QUANTITATIVE DETERMINATION OF HUMAN CTGF CONCENTRATIONS IN SERUM AND PLASMA ELISA NAME Catalog

More information

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VEGF in cell culture supernates, serum, and plasma (heparin, EDTA, citrate).

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human VEGF in cell culture supernates, serum, and plasma (heparin, EDTA, citrate). GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Human VEGF ELISA Kit Catalog Number: Size: GWB-ZZK029

More information

Rat α-melanocyte stimulating hormone (α-msh) ELISA Kit

Rat α-melanocyte stimulating hormone (α-msh) ELISA Kit Rat α-melanocyte stimulating hormone (α-msh) ELISA Kit For the quantitative determination of rat α-melanocyte stimulating hormone (α-msh) concentrations in serum, plasma, tissue homogenates. This package

More information

ab46042 IL-6 (Interleukin-6) High Sensitivity Human ELISA Kit

ab46042 IL-6 (Interleukin-6) High Sensitivity Human ELISA Kit Version 5 Last updated 8 September 2017 ab46042 IL-6 (Interleukin-6) High Sensitivity Human ELISA Kit For the quantitative measurement of IL-6 (Interleukin-6) in supernatants, buffered solutions, serum,

More information

Human Serum Albumin Assay

Human Serum Albumin Assay Human Serum Albumin Assay Immunoenzymetric Assay for the Measurement of Human Serum Albumin Catalog # F055 Intended Use This kit is intended for use in quantitating human albumin (HSA). The kit is for

More information

Porcine IgM (Immunoglobulin M) ELISA Kit

Porcine IgM (Immunoglobulin M) ELISA Kit Porcine IgM (Immunoglobulin M) ELISA Kit Catalogue No: EP0085 Size: 48T/96T Reactivity: Porcine Detection Range: 0.156-10ng/ml Sensitivity:

More information

PeliClass human IgG subclass ELISA kit Enzyme-linked immunosorbent assay

PeliClass human IgG subclass ELISA kit Enzyme-linked immunosorbent assay PeliClass human IgG subclass ELISA kit Enzyme-linked immunosorbent assay Catalog No: M1551 Size: six pre-coated 8-well strips for each of the four IgG subclasses Test description The PeliClass human subclass

More information

Rat Creatinine (Cr) ELISA Kit

Rat Creatinine (Cr) ELISA Kit Rat Creatinine (Cr) ELISA Kit Catalog Number. CSB-E16687r For the quantitative determination of endogenic rat creatinine (Cr) concentrations in serum, plasma, cell culture supernates, tissue homogenates

More information

Human immunoglobulin G(IgG) ELISA Kit

Human immunoglobulin G(IgG) ELISA Kit Human immunoglobulin G(IgG) ELISA Kit For the quantitative determination of human immunoglobulin G (IgG) concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates, cell lysates.

More information

MOUSE ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT

MOUSE ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT PAGE 1 MOUSE ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF MOUSE ESM-1 CONCENTRATIONS IN CELL CULTURE SUPERNATES, SERUM AND EDTA PLASMA ALWAYS REFER TO

More information

Bovine Prostaglandin E2 (PG-E2) ELISA Kit

Bovine Prostaglandin E2 (PG-E2) ELISA Kit Bovine Prostaglandin E2 (PG-E2) ELISA Kit Catalog Number. CSB-E14237B For the quantitative determination of endogenic bovine prostaglandin E2 (PG-E2) concentrations in serum, plasma, tissue homogenates.

More information

Guinea Pig IgA ELISA Kit

Guinea Pig IgA ELISA Kit CATALOG NO: IGPIGAKT Guinea Pig IgA ELISA Kit LOT NO: SAMPLE INTENDED USE The IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in biological samples of

More information

Human IL-1 alpha ELISA Kit

Human IL-1 alpha ELISA Kit AssayMax TM Human IL-1 alpha ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Mouse TNF alpha ELISA Kit

Mouse TNF alpha ELISA Kit Mouse TNF alpha ELISA Kit Catalog No. GWB-ZZD049 Size 96 wells/kit Sandwich ELISA kit for quantitative detection of mouse TNF alpha in cell culture supernates, serum and plasma(heparin, EDTA). Typical

More information

Global Headquarters 86 Cummings Park Woburn, MA Tel:

Global Headquarters 86 Cummings Park Woburn, MA Tel: Human beta-defensin 1 ELISA Kit Cat:RK00211 This ELISA kit used for quantitation of human Defensin Beta 1 (BD-1) concentration in cell culture supernate, serum and plasma. For research use only, and it

More information

COLORIMETRIC SANDWICH ELISA KIT INSTRUCTION MANUAL

COLORIMETRIC SANDWICH ELISA KIT INSTRUCTION MANUAL Page 1 of 7 COLORIMETRIC SANDWICH ELISA KIT INSTRUCTION MANUAL This product is for research use ONLY and not for human or animal therapeutic or diagnostic use. Page 2 of 7 Contents Page 3 I. Supplied Materials:

More information

1 MICROTITER PLATE 96 wells 2 ENZYME CONJUGATE 10.0 ml 1 vial 3 STANDARD.1 0 pg/ml 1 vial 4 STANDARD pg/ml 1 vial 5 STANDARD.

1 MICROTITER PLATE 96 wells 2 ENZYME CONJUGATE 10.0 ml 1 vial 3 STANDARD.1 0 pg/ml 1 vial 4 STANDARD pg/ml 1 vial 5 STANDARD. Rabbit Thyrotropin Releasing Hormone (TRH) Elisa Kit 96 Tests Catalog Number: MBS725153 Store all reagents at 2-8 C Valid Period:six months FOR LABORATORY RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC

More information

HUMAN ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT

HUMAN ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT PAGE 1 HUMAN ENDOTHELIAL- CELL SPECIFIC MOLECULE- 1 (ESM-1) ELISA KIT FOR THE QUANTITATIVE DETERMINATION OF HUMAN ESM-1 CONCENTRATIONS IN SERUM AND EDTA PLASMA ALWAYS REFER TO LOT SPECIFIC PROTCOL PROVIDED

More information

Human connective tissue growth factor (CTGF) ELISA Kit. MyBioSource.com. This package insert must be read in its entirety before using this product.

Human connective tissue growth factor (CTGF) ELISA Kit. MyBioSource.com. This package insert must be read in its entirety before using this product. Human connective tissue growth factor (CTGF) ELISA Kit Catalog Number. For the quantitative determination of human connective tissue growth factor (CTGF) concentrations in serum, plasma, tissue homogenates.

More information

ab Thrombin (Factor II) Human ELISA Kit

ab Thrombin (Factor II) Human ELISA Kit ab108909 Thrombin (Factor II) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thrombin (Factor II) in cell culture supernatants. This product is for research use only and

More information

Rat TNF-alpha ELISA Kit

Rat TNF-alpha ELISA Kit AssayMax TM Rat TNF-alpha ELISA Kit Assaypro LLC 3400 Harry S Truman Blvd St. Charles, MO 63301 T (636) 447-9175 F (636) 395-7419 www.assaypro.com For any questions regarding troubleshooting or performing

More information

Human protein kinase C beta II (PKC-bII) ELISA Kit

Human protein kinase C beta II (PKC-bII) ELISA Kit Human protein kinase C beta II (PKC-bII) ELISA Kit Catalog Number. CSB-E15925h For the quantitative determination of human protein kinase C beta II (PKC-bII) concentrations in serum, plasma, tissue homogenates,

More information

Corticosterone ELISA Kit

Corticosterone ELISA Kit Corticosterone ELISA Kit Catalog Number KA0468 96 assays Version: 31 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General

More information

RayBio Human Caspase-3 ELISA Kit

RayBio Human Caspase-3 ELISA Kit RayBio Human Caspase-3 ELISA Kit Catalog #: ELH-CASP3 User Manual Last revised July 21, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,

More information

Rat TNF-alpha ELISA Kit(KT20661) User Manual. For research use only. Not intended for diagnostic testing.

Rat TNF-alpha ELISA Kit(KT20661) User Manual. For research use only. Not intended for diagnostic testing. Rat TNF-alpha ELISA Kit(KT20661) User Manual For research use only. Not intended for diagnostic testing. www.abgent.com TABLE OF CONTENTS I. Introduction...2 II. Reagents.... 2 III. Storage.. 3 IV. Additional

More information

Your Analyte ELISA Kit Instruction

Your Analyte ELISA Kit Instruction NovaTeinBio FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC PURPOSES Your Analyte ELISA Kit Instruction Intended use The kit is used to detect the level of Your analyte in cell culture, serum blood plasma and

More information

General protocol for ENZYME IMMUNOASSAY KIT (range: 0-25ng/ml)

General protocol for ENZYME IMMUNOASSAY KIT (range: 0-25ng/ml) General protocol for ENZYME IMMUNOASSAY KIT (range: 0-25ng/ml) PHOENIX PHARMACEUTICALS, INC. INTRODUCTION This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based

More information

Human Total tpa ELISA Kit

Human Total tpa ELISA Kit Human Total tpa ELISA Kit Vertrieb: L O X O GmbH Immunbiologie Biochemie, Produkte und Systeme Postfach 11 30 69215 Dossenheim Telefon +49 (0) 62 21-86 80 23 FAX +49 (0) 62 21-86 80 255 E-Mail: info@loxo.de

More information

Rat Tumor Necrosis Factor Alpha (TNF-α) ELISA

Rat Tumor Necrosis Factor Alpha (TNF-α) ELISA Rat Tumor Necrosis Factor Alpha (TNF-α) ELISA Catalog Number M046039 For the quantitative determination of TNF-α in rat cell culture supernate samples. For research use only. This product insert must be

More information

Human myelin basic protein(mbp) antibody ELISA Kit

Human myelin basic protein(mbp) antibody ELISA Kit Human myelin basic protein(mbp) antibody ELISA Kit Catalog Number.... For the quantitative determination of human myelin basic protein (MBP) antibody concentrations in serum, cerebrospinal fluid (CSF).

More information

ab Albumin Human ELISA Kit

ab Albumin Human ELISA Kit ab108787 Albumin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Albumin in plasma and serum. This product is for research use only and is not intended for diagnostic use.

More information

Canine Fibrinogen ELISA

Canine Fibrinogen ELISA Canine Fibrinogen ELISA For the quantitative determination of Fibrinogen in canine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 41-FIBCA-E01 Size: 96 wells

More information

Human TGF-beta1 ELISA

Human TGF-beta1 ELISA K-ASSAY Human TGF-beta1 ELISA For the quantitative determination of TGF-beta1 in human cell culture supernates, serum, plasma (EDTA) and urine Cat. No. KT-1471 For Research Use Only. Not for diagnostic

More information

Human immunoglobulin G(IgG) ELISA Kit

Human immunoglobulin G(IgG) ELISA Kit Human immunoglobulin G(IgG) ELISA Kit Catalog Number. CSB-E07979h For the quantitative determination of human immunoglobulin G (IgG) concentrations in serum, plasma, cell culture supernates, urine, tissue

More information

ab Porcine VEGF-A ELISA Kit

ab Porcine VEGF-A ELISA Kit Version 1 Last updated 27 January 2017 ab218298 Porcine VEGF-A ELISA Kit For the quantitative measurement of porcine VEGF-A in serum, plasma and cell culture supernatants. This product is for research

More information

Human CNTF ELISA Kit

Human CNTF ELISA Kit Human CNTF ELISA Kit Catalog No. K0331254 Lot No. 31202 Quantity 96 tests Storage 4 C Standard Range 31.25 2000 pg/ml [Important Notice] Please read this User Manual carefully prior to performing the assay.

More information

Protocol for Catalog # FEK BNP-45 (Mouse) FLUORESCENT IMMUNOASSAY KIT (range: 0-10,000pg/ml)

Protocol for Catalog # FEK BNP-45 (Mouse) FLUORESCENT IMMUNOASSAY KIT (range: 0-10,000pg/ml) Protocol for Catalog # FEK-011-23 BNP-45 (Mouse) FLUORESCENT IMMUNOASSAY KIT (range: 0-10,000) PHOENIX PHARMACEUTICALS, I NC. INTRODUCTION This Fluorescent Immunoassay kit is designed to detect a specific

More information

Bovine Serum Albumin (BSA) Assay

Bovine Serum Albumin (BSA) Assay Bovine Serum Albumin (BSA) Assay Immunoenzymetric Assay for the Measurement of BSA Catalog # F030 Intended Use This kit is intended for use in quantitating bovine serum albumin (BSA). The kit is for Research

More information

HBeAg and HBeAg Ab ELISA Kit

HBeAg and HBeAg Ab ELISA Kit HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General

More information

Corticosterone ELISA Kit

Corticosterone ELISA Kit Corticosterone ELISA Kit Catalog Number KA0468 96 assays Version: 40 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General

More information

Human Peptide YY EIA

Human Peptide YY EIA KAMIYA BIOMEDICAL COMPANY Human Peptide YY EIA For the quantitative determination of Peptide YY in human serum and plasma. Cat. No. KT-378 For Research Use Only. 1 Rev. 9822378 PRODUCT INFORMATION Human

More information

Porcine Transferrin Receptor(TFR) ELISA Kit

Porcine Transferrin Receptor(TFR) ELISA Kit Porcine Transferrin Receptor(TFR) ELISA Kit Catalog No. CSB-E13481p (96T) This immunoassay kit allows for the in vitro quantitative determination of porcine TFR concentrations in serum, plasma. Expiration

More information

Human alpha-2-hs- Glycoprotein ELISA Kit

Human alpha-2-hs- Glycoprotein ELISA Kit Vertrieb: L O X O GmbH Immunbiologie Biochemie, Produkte und Systeme Postfach 11 30 69215 Dossenheim Telefon +49 (0) 62 21-86 80 23 FAX +49 (0) 62 21-86 80 255 E-Mail: info@loxo.de Internet: www.loxo.de

More information

IDTox Lactate Dehydrogenase (LDH) Enzyme Cytotoxicity Kit For Cell Culture Supernatant Samples

IDTox Lactate Dehydrogenase (LDH) Enzyme Cytotoxicity Kit For Cell Culture Supernatant Samples Rev Date: Mar 26 2015 Rev: 1 1 of 1 IDTox Lactate Dehydrogenase (LDH) Enzyme Cytotoxicity Kit For Cell Culture Supernatant Samples 192 Wells Enzyme Immunoassay for the determination of the Lactate Dehydrogenase

More information

Human MMP-1 ELISA Kit

Human MMP-1 ELISA Kit INSTRUCTIONS Human MMP-1 ELISA Kit EHMMP1 Number EHMMP1 Description Human MMP-1 ELISA Kit, sufficient reagents for 96 determinations Table of Contents Kit Contents Anti-Human MMP-1 Precoated 96-well Strip

More information