The Study of the Effect of Drugs of Abuse on Protein Kinase A Activity in Mouse Brain and Spinal Cord
|
|
- Penelope Anna Wright
- 6 years ago
- Views:
Transcription
1 Virginia Commonwealth University VCU Scholars Compass Theses and Dissertations Graduate School 2005 The Study of the Effect of Drugs of Abuse on Protein Kinase A Activity in Mouse Brain and Spinal Cord George D. Dalton Virginia Commonwealth University Follow this and additional works at: Part of the Medical Pharmacology Commons The Author Downloaded from This Dissertation is brought to you for free and open access by the Graduate School at VCU Scholars Compass. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of VCU Scholars Compass. For more information, please contact libcompass@vcu.edu.
2
3
4
5
6 iv Materials Methods of handling mice Surgical implantation of morphine pellets Chronic treatment with A9-THC Intracerebroventricular injections Intrathecal injections Models of morphine antinociceptive tolerance The tail-flick test 33 Reversal of morphine tolerance and i.c.v. injections scheme Potency and selectivity of PKA inhibitors Measurement of PKA activity 35 Activation of PKA by Sp-8-Br-CAMPS Data analysis of PKA activity The correlation of the reversal of morphine antinociceptive tolerance and the inhibition of neuronal Protein Kinase A activity by a Protein Kinase A... inhibitor Background 39 Results Discussion The effect of the level of morphine and A9-THC antinociceptive Tolerance on Protein Kinase A activity in mouse brain and spinal cord Background Results Discussion... 80
7 The effect of the duration of morphine antinociceptive tolerance on Protein Kinase A activity in mouse brain and spinal cord Background Results Discussion 103 General Discussion List of References. 121
8 LIST OF TABLES Table Page 1. Opioid receptor ligands The reversal of morphine antinociceptive tolerance by PKI-(6-22)-amide and PKI-Myr-(14-22)-amide In vitro effect of PKI-(6-22)-amide and PKI-Myr-(14-22)-amide on Protein Kinase A activity in cytosolic and particulate fractions in mouse brain and spinal cord Effect of i.c.v. administration of PKI-(6-22)-amide and PKI-Myr-(14-22)- amide on Protein Kinase A activity in cytosolic and particulate fractions of drug-nai've mouse thalamus and periaqueductal gray Effect of i.c.v. administration of PKI-(6-22)-amide and PKI-Myr-(14-22)- amide on Protein Kinase A activity in cytosolic and particulate fractions of drug-nai've mouse medulla and lumbar spinal cord Effect of i.c.v. administration of PKI-(6-22)-amide and PKI-Myr-(14-22)- amide on Protein Kinase A activity in cytosolic and particulate fractions of 3-day morphine tolerant mouse thalamus and periaqueductal gray Effect of i.c.v. administration of PKI-(6-22)-amide and PKI-Myr-(14-22)- amide on Protein Kinase A activity in cytosolic and particulate fractions of 3-day morphine tolerant mouse medulla and lumbar spinal cord Measurement of basal and total cytosolic Protein Kinase A activity in drug-nai've mouse thalamus and periaqueductal gray immediately following the i.c.v. administration of PKI-(6-22)-amide and PKI-Myr-(14-22)-amide Induction of tolerance to the antinociceptive effects of A'-THC Effects of chronic treatment with A'-THC on cytosolic Protein Kinase A activity in various mouse brain regions and lumbar spinal cord... 73
9
10
11
12 Abstract THE STUDY OF THE EFFECT OF DRUGS OF ABUSE ON PROTEIN KINASE A ACTIVITY IN MOUSE BRAIN AND SPINAL CORD. By George D. Dalton, Ph.D. A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University. Virginia Commonwealth University, Dr. William L. Dewey, Professor, Department of Pharmacology & Toxicology Morphine and A9-THC are drugs that produce analgesia and rewarding effects. However, chronic treatment with morphine and a9-thc produces problematic side-effects including tolerance and physical dependence. The cellular mechanisms underlying opioid and cannabinoid antinociceptive tolerance have been studied for years. Research has demonstrated that the expression of morphine and A~-THC antinociceptive tolerance may be mediated through intracellular signaling pathways, such as the adenylyl cyclase /Protein Kinase A (PKA) cascade. The present study investigated the role of PKA in the expression of morphine and a9-thc antinociceptive tolerance. Male Swiss Webster mice were treated chronically with morphine or A~-THC and the warm-water tail-flick test was used to assess antinociception. These studies revealed that the level and the duration of morphine antinociceptive tolerance both influenced whether
13 PKA activity was increased in mouse brain and spinal cord. Cytosolic PKA activity was increased in the thalamus of 3-day morphine-tolerant mice expressing a 45-fold level of tolerance, but not in mice that expressed a 10-fold level of tolerance. In addition, cytosolic PKA activity was increased in the lumbar spinal cord (LSC) of 15-day morphine-tolerant mice. However, chronic treatment with A9-THC had no effect on neuronal PKA activity even in mice that expressed a high level of antinociceptive tolerance. The absence of an effect of chronic treatment with A9-THC on neuronal PKA activity was supported by the development of a positive control in which the PKA activator Sp-8-Br-CAMPS was administered intracerebroventricularly (i.c.v.) and intrathecally (i.t.) in drug-nayve mice and increases in PKA activity were observed in several brain regions and LSC. Finally, the i.c.v. injection of two peptide fragments of native Protein Kinase A inhibitor (PKI) peptide, PKI-(6-22)-amide and PKI-(Myr )- amide, significantly reversed antinociceptive tolerance in mice treated chronically with morphine. PKI-(6-22)-amide (i.c.v.) also inhibited PKA activity in brain regions (thalamus, periaqueductal gray (PAG), and medulla) and LSC, which studies have shown play a role in morphine-induced analgesia. Moreover, PKI-(6-22)-amide reduced the increase in PKA activity in thalamus and LSC observed with chronic morphine treatment. Overall, these studies provide evidence that PKA plays a role in morphine tolerance, but not A~-THC tolerance at the doses and times tested.
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
7.06 Cell Biology QUIZ #3
Recitation Section: 7.06 Cell Biology QUIZ #3 This is an open book exam, and you are allowed access to books and notes, but not computers or any other types of electronic devices. Please write your answers
More informationAcid-Sensing Ion Channels
Acid-Sensing Ion Channels Properties of the H + -activated channel. a. Examples of ion currents at 70 mv caused by a rapid change from ph 7.4 to 6.0. b. ph response curves showing current change normalized
More informationTIDES 2014 GalNAc-siRNA with Enhanced Stabilization Chemistry: ESC-GalNAc-siRNA. May 14, 2014 Muthiah Manoharan
TIDES 2014 GalNAc-siRNA with Enhanced Stabilization Chemistry: ESC-GalNAc-siRNA May 14, 2014 Muthiah Manoharan Agenda sirna-galnac Conjugates: Background Standard Template Chemistry (STC)» Human POC of
More informationCRE/CREB Reporter Assay Kit camp/pka Cell Signaling Pathway Catalog #: 60611
Data Sheet CRE/CREB Reporter Assay Kit camp/pka Cell Signaling Pathway Catalog #: 60611 Background The main role of the camp response element, or CRE, is mediating the effects of Protein Kinase A (PKA)
More informationPolicy 19 Fluid Administration and Collection in Rodents Version 1.0 Approval Date: 12/12/12
Policy 19 Fluid Administration and Collection in Rodents Version 1.0 Approval Date: 12/12/12 Purpose This policy was designed to provide investigators with reference values related to the administration
More informationPUBLICATIONS: (1-29) 1. Rosen, K. M., Moussa, C. E., Lee, H. K., Kumar, P., Kitada, T., Qin, G., Fu, Q., and Querfurth, H. W. (2009) Parkin reverses
PUBLICATIONS: (1-29) 1. Rosen, K. M., Moussa, C. E., Lee, H. K., Kumar, P., Kitada, T., Qin, G., Fu, Q., and Querfurth, H. W. (2009) Parkin reverses intracellular beta-amyloid accumulation and its negative
More informationFlashPlate File #15. High Throughput Screening. J. Watson SmithKline Beecham Pharmaceuticals, UK.
Drug Discovery Research Clinical Screening High Throughput Screening FlashPlate File #15 Use of Novel FlashPlate Technology to Measure camp Accumulation in Chinese Hamster Ovary Cells Expressing Human
More informationEffects of serine/threonine protein phosphatase inhibitors on morphine-induced antinociception in the tail flick test in mice
European Journal of Pharmacology 465 (2003) 53 60 www.elsevier.com/locate/ejphar Effects of serine/threonine protein phosphatase inhibitors on morphine-induced antinociception in the tail flick test in
More informationEXECUTIVE SUMMARY GLOBAL GENOMICS AND BIOINFORMATICS RESEARCH INSTITUTE. May 24, 2017
EXECUTIVE SUMMARY GLOBAL GENOMICS AND BIOINFORMATICS RESEARCH INSTITUTE May 24, 2017 Introduction to the Institute Inova Health System Foundation ( Inova ), The Rector and Visitors of the University of
More informationOpioid-induced tolerance and dependence in mice is modulated by the distance between pharmacophores in a bivalent ligand series
Opioid-induced tolerance and dependence in mice is modulated by the distance between pharmacophores in a bivalent ligand series David J. Daniels*, Natalie R. Lenard, Chris L. Etienne*, Ping-Yee Law, Sandra
More informationSolution Key Problem Set
Solution Key- 7.013 Problem Set 5-2013 Question 1 During a summer hike you suddenly spot a huge grizzly bear. This emergency situation triggers a fight or flight response through a signaling pathway as
More informationCOMMITTEE FOR MEDICINAL PRODUCTS FOR HUMAN USE (CHMP) GUIDELINE ON THE NON-CLINICAL DEVELOPMENT OF FIXED COMBINATIONS OF MEDICINAL PRODUCTS
European Medicines Agency London, 24 January 2008 Doc. Ref. EMEA/CHMP/SWP/258498/2005 COMMITTEE FOR MEDICINAL PRODUCTS FOR HUMAN USE (CHMP) GUIDELINE ON THE NON-CLINICAL DEVELOPMENT OF FIXED COMBINATIONS
More informationQualifying Examination (Part I)
Qualifying Exam December 2011 Department of Pharmacology Qualifying Examination (Part I) December 13-15, 2011 Tuesday, December 13, 2011 Wednesday, December 14, 2011 Thursday, December 15, 2011 EXAM SCHEDULE
More informationWhite Paper Authentic Recombinant Activin A and BMP- 4 Production Using HumanZyme s Proprietary Human Cell Line Expression Technology
White Paper Authentic Recombinant Activin A and BMP- 4 Production Using HumanZyme s Proprietary Human Cell Line Expression Technology Mark Azam, Ph.D. VP - R&D and Manufacturing Patricia Ahrweiler, Ph.D.
More informationCindy L. Stanfield. University of South Alabama 5721 USA Drive North, Room 4004 Mobile, AL (251) Fax: (251)
Cindy L. Stanfield PERSONAL INFORMATION Birth: January 6, 1962 Castro Valley, California Home Address: Work Address: 4580 Cavalier Drive Semmes, AL 36575 (251) 490-1152 Pre-health Advising University of
More informationSIMS2003. Instructors:Rus Yukhananov, Alex Loguinov BWH, Harvard Medical School. Introduction to Microarray Technology.
SIMS2003 Instructors:Rus Yukhananov, Alex Loguinov BWH, Harvard Medical School Introduction to Microarray Technology. Lecture 1 I. EXPERIMENTAL DETAILS II. ARRAY CONSTRUCTION III. IMAGE ANALYSIS Lecture
More informationThe Confo body technology, a new platform to enable fragment screening on GPCRs
The Confo body technology, a new platform to enable fragment screening on GPCRs Christel Menet, PhD CSO Miptec, 216 Confo Therapeutics Incorporated in June 215 Located in Brussels, Belgium Confo Therapeutics
More information7.013 Practice Quiz
MIT Department of Biology 7.013: Introductory Biology - Spring 2005 Instructors: Professor Hazel Sive, Professor Tyler Jacks, Dr. Claudette Gardel 7.013 Practice Quiz 2 2004 1 Question 1 A. The primer
More informationEvidence for the Involvement of Descending Pain-Inhibitory Mechanisms in the Antinociceptive Effect of Hecogenin Acetate
pubs.acs.org/jnp Evidence for the Involvement of Descending Pain-Inhibitory Mechanisms in the Antinociceptive Effect of Hecogenin Acetate Kelly Barbosa Gama, Jullyana S. S. Quintans, A ngelo R. Antoniolli,
More informationBiomedical Sciences (Medical) (BMSC)
Biomedical Sciences (Medical) (BMSC) 1 Biomedical Sciences (Medical) (BMSC) Courses BMSC 8001. Introduction to Biochemistry and Molecular Biology. 3 Credit Hours. Graduate level course that describes fundamentals
More informationUse of Antisense Oligonucleotides for the Treatment of Inheritable Rare Disorders. C. Frank Bennett Isis Pharmaceuticals
Use of Antisense ligonucleotides for the Treatment of Inheritable Rare Disorders C. Frank Bennett Isis Pharmaceuticals Agenda Review different antisense strategies Delivery of oligonucleotides to the skin,
More informationMedicinal Chemistry: Principles and Practice
Medicinal Chemistry: Principles and Practice Edited by Frank D. King SmithKline Beecham Pharmaceuticals, Harlow, UK ^ THE ROYAL SOCIETY OF CHEMISTRY Contents Chapter 1 An Introduction to Drug-Receptor
More informationThe World Leader in SPR Technology. Jimmy Page, PhD, Biacore, Inc.
The World Leader in SPR Technology Jimmy Page, PhD, Biacore, Inc. Objectives of Biacore Experiments Yes/No Data» Is there binding?» Ligand Fishing Concentration Analysis: How MUCH? Active Concentration
More informationReadings. Lecture IV. Mechanisms of Neural. Neural Development. September 10, Bio 3411 Lecture IV. Mechanisms of Neural Development
Readings Lecture IV. Mechanisms of Neural NEUROSCIENCE: References : 5 th ed, pp 477-506 (sorta) 4 th ed, pp 545-575 (sorta) Fainsod, A., Steinbeisser, H., & De Robertis, E. M. (1994). EMBO J, 13(21),
More informationScience June 3, 1988 v240 n4857 p1310(7) Page 1
Science June 3, 1988 v240 n4857 p1310(7) Page 1 by Brian K. Kobilka, Tong Sun Kobilka, Kiefer Daniel, John W. Regan, Marc G. Caron and Robert J. Lefkowitz COPYRIGHT 1988 American Association for the Advancement
More informationNature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 PCR-genotyping of the three mouse models used in this study and controls for behavioral experiments after semi-chronic Pten inhibition. a-c. DNA from App/Psen1 (a), Pten tg (b) and
More informationA specific antidote for reversal of anticoagulation by direct and indirect inhibitors of coagulation factor Xa
A specific antidote for reversal of anticoagulation by direct and indirect inhibitors of coagulation factor Xa Lu G, Deguzman FR, Hollenbach SJ, et al. Department of Biology, Portola Pharmaceuticals Inc.,
More informationProfiling Ligands Of GPCR Targets Using An Optical Biosensor With Dynamic Mass Redistribution Technology
Profiling Ligands Of GPCR Targets Using An Optical Biosensor With Dynamic Mass Redistribution Technology Paul Lee, Ph.D. Lead Discovery Amgen Inc. Thousand Oaks, CA 2008 Label-Free Summit, Corning NY October
More informationMathematical modelling in drug discovery
Mathematical modelling in drug discovery Garrit Jentsch Computational Biology, Discovery Sciences, AstraZeneca October 17th, 2012 Who are we? http://www.astrazeneca.co.uk/astrazeneca-in-uk/our-uk-sites/alderley-park
More informationAn Integrated Approach to PK/PD/IG/Safety for Biologics
An Integrated Approach to PK/PD/IG/Safety for Biologics Moderator: Sanela Bilic, PharmD, MBA Novartis Oncology, Clinical Pharmacology Speakers: Sebastian Spindledreher, PhD, DMPK-Bx, Novartis AG Jennifer
More informationImplantation of AtT-20 or Genetically Modified AtT=20/hENK Cells in Mouse Spinal Cord Induced Antinociception and Opioid Tolerance
The Journal of Neuroscience, August 1994, 14(8): 4808-%814 Implantation of AtT-20 or Genetically Modified AtT=20/hENK Cells in Mouse Spinal Cord Induced Antinociception and Opioid Tolerance Hope H. Wu,
More informationMICB688L/MOCB639 Advanced Cell Biology Exam II
MICB688L/MOCB639 Advanced Cell Biology Exam II May 10, 2001 Name: 1. Briefly describe the four major classes of cell surface receptors and their modes of action (immediate downstream only) (8) 2. Please
More informationDNA Binding Domains: Structural Motifs. Effector Domain. Zinc Fingers. Zinc Fingers, continued. Zif268
DNA Binding Domains: Structural Motifs Studies of known transcription factors have found several motifs of protein design to allow sequence-specific binding of DNA. We will cover only three of these motifs:
More informationBiology Eighth Edition Neil Campbell and Jane Reece
BIG IDEA IV Biological systems interact, and these systems and their interactions possess complex properties. Enduring Understanding 4.B Competition and cooperation are important aspects of biological
More informationCharles Shuler, Ph.D. University of Southern California Los Angeles, California Approved for Public Release; Distribution Unlimited
AD Award Number: DAMD17-01-1-0100 TITLE: Smad-Mediated Signaling During Prostate Growth and Development PRINCIPAL INVESTIGATOR: Charles Shuler, Ph.D. CONTRACTING ORGANIZATION: University of Southern California
More informationAntisense Therapeutics Ltd ASX:ANP January 2017
Antisense Therapeutics Ltd ASX:ANP January 2017 Forward Looking Statements This presentation contains forward-looking statements regarding the Company s business and the therapeutic and commercial potential
More informationa) JOURNAL OF BIOLOGICAL CHEMISTRY b) PNAS c) NATURE
a) JOURNAL OF BIOLOGICAL CHEMISTRY b) c) d) ........................ JOURNAL OF BIOLOGICAL CHEMISTRY MOLECULAR PHARMACOLOGY TRENDS IN PHARMACOLOGICAL S AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY
More informationStem cells and motor neurone disease
Stem cells and motor neurone disease F Stem cell research has fuelled hope of a treatment for a variety of conditions. This information sheet explains what these cells are and includes details of the current
More informationTECHNICAL BULLETIN. MEK Activity Assay Kit. Product Code CS0490 Storage Temperature 20 C
MEK Activity Assay Kit Product Code CS0490 Storage Temperature 20 C TECHNICAL BULLETIN Product Description The MAP kinase kinases (MAPKK, mitogen-activated protein kinase kinase, also termed MEK) are a
More information7.22 Example Problems for Exam 1 The exam will be of this format. It will consist of 2-3 sets scenarios.
Massachusetts Institute of Technology Department of Biology 7.22, Fall 2005 - Developmental Biology Instructors: Professor Hazel Sive, Professor Martha Constantine-Paton 1 of 10 7.22 Fall 2005 sample exam
More informationSureSilencing sirna Array Technology Overview
SureSilencing sirna Array Technology Overview Pathway-Focused sirna-based RNA Interference Topics to be Covered Who is SuperArray? Brief Introduction to RNA Interference Challenges Facing RNA Interference
More informationHomology Modeling of Mouse orphan G-protein coupled receptors Q99MX9 and G2A
Quality in Primary Care (2016) 24 (2): 49-57 2016 Insight Medical Publishing Group Research Article Homology Modeling of Mouse orphan G-protein Research Article Open Access coupled receptors Q99MX9 and
More informationStrategy for Selecting NAb Assay Format
Strategy for Selecting NAb Assay Format European Bioanalysis Forum 27Sept2016 Jim McNally, Ph.D. Associate Director, Global Early Development Head of Clinical Bioanalytics Merck KGaA AAPS Working Group
More informationCHARACTERIZATION OF SELECTIVE INHIBITION OF ADENYLYL CYCLASE ACTIVITY BY SMALL MOLECULE INHIBITOR NKY80
Texas Medical Center Library DigitalCommons@TMC UT GSBS Dissertations and Theses (Open Access) Graduate School of Biomedical Sciences 12-2012 CHARACTERIZATION OF SELECTIVE INHIBITION OF ADENYLYL CYCLASE
More informationThe Pathways to Understanding Diseases
FOR PHARMA & LIFE SCIENCES White paper The Pathways to Understanding Diseases Deciphering complex biological processes Executive Summary Understanding complex biological processes is critical whether doing
More informationFlow CAST : Testing Potency and Efficacy of Inhibitors of PI3K δ, PI3Kγ, BTK and SYK Activity
Flow CAST : Testing Potency and Efficacy of Inhibitors of PI3K δ, PI3Kγ, BTK and SYK Activity Michele Romano, PhD Product Manager Flow CAST is for Research Use Only. Not for use in diagnostic procedures.
More informationMurine TGFβ-antagonist (RAP-1332) Inhibits Fibrosis in a Murine Model of Myelofibrosis. Rajasekhar NVS Suragani, PhD
Murine TGFβ-antagonist (RAP-1332) Inhibits Fibrosis in a Murine Model of Myelofibrosis Rajasekhar NVS Suragani, PhD Polycythemia Vera (PV), Essential Thrombocythemia (ET) and Primary Myelofibrosis (PMF)
More informationPharmacology. Chatchai Chinpaisal, Ph.D. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Silpakorn University.
Pharmacology Chatchai Chinpaisal, Ph.D. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Silpakorn University. 1 PHARMACODYNAMIC STUDIES A. Primary pharmacodynamics primary action in target
More informationYellow Fever Vaccine (Live) is a freeze-dried preparation of the 17D strain of yellow fever virus grown in fertilised hen eggs.
YELLOW FEVER VACCINE Yellow Fever Vaccine (Live) is a freeze-dried preparation of the 17D strain of yellow fever virus grown in fertilised hen eggs. Production General provisions The production of vaccine
More informationRecombinant Biglycan for the Treatment! of Duchenne Muscular Dystrophy!!! PPMD Annual Conference, Jun 2013! By Joel B. Braunstein, MD!
Recombinant Biglycan for the Treatment! of Duchenne Muscular Dystrophy!!! PPMD Annual Conference, Jun 2013! By Joel B. Braunstein, MD! Tivorsan Overview Commercial operations began in 2010 around biglycan
More informationType: Embedded within the selected substance, indicated when creating the substance.
Overview: Substance Table: Each procedure will include a substance table, either on the main page of the procedure (immediately after selecting the procedure type), embedded elsewhere within a procedure,
More informationWhat s the difference? Challenges in pre-clinical development of biologics
Biologics vs Small MW NCEs What s the difference? Challenges in pre-clinical development of biologics Peter Lloyd Joint Conference of EU Human Pharmacological Societies and 20 th Anniversary of AGAH 31
More informationThe Making of the Fittest: Natural Selection and Adaptation
INTRODUCTION THE ROCK POCKET MOUSE THE BIOCHEMISTRY AND CELL SIGNALING PATHWAY OF MC1R The rock pocket mouse, Chaetodipus intermedius, is a small, nocturnal animal found in the deserts of the southwestern
More informationTogether to Compete Stefano Manfredini
University of Ferrara Together to Compete Stefano Manfredini Converting research results into economic and social values Creative thinking Invention + ACTIO IOV-ATIO
More informationZ -LYTE KINASE ASSAY KIT SER/THR 9 PEPTIDE PROTOCOL
Z -LYTE KINASE ASSAY KIT SER/THR 9 PEPTIDE PROTOCOL Part # PV3324 Lit. # 762-038412 020304 TABLE OF CONTENTS 1.0 INTRODUCTION... 2 2.0 ASSAY THEORY... 2 3.0 Z -LYTE KINASE ASSAY KIT SER/THR 9 PEPTIDE MATERIALS
More informationNEN Life Science Products
NEN Life Science Products Adenylyl Cyclase Activation FlashPlate Assay A Direct camp [ 125 I] Detection Assay Catalog Numbers SMP004, 2-plate kit SMP004A, 5-plate kit For Laboratory Use Only. Caution:
More informationCorning Epic System. Applications. Therapeutic Application
Corning Epic System Applications The Corning Epic System can be applied to probe many of the biomolecular interactions involved in cellular and molecular biology. Beyond its application to direct binding,
More informationKarnam Follow this S. Murthy and additional works at:
Virginia Commonwealth University VCU Scholars Compass Physiology and Biophysics Publications Dept. of Physiology and Biophysics 2013 Distinctive G Protein-Dependent Signaling by Protease-Activated Receptor
More informationWe are committed to translating ground-breaking science into genomic therapies that transform patients lives
We are committed to translating ground-breaking science into genomic therapies that transform patients lives Liver-based expression of the human alpha-galactosidase A gene (GLA) in a murine Fabry model
More informationSafety Assessment of Oligonucleotide Constructs. Scott P. Henry, VP, Preclinical Development, Isis Pharmaceuticals, Inc., USA
Safety Assessment of ligonucleotide Constructs Scott P. Henry, VP, Preclinical Development, Isis Pharmaceuticals, Inc., USA Drug Development of 2 ME AS Platform Technology 2 Platform technologies offer
More informationBL-7040: Oligonucleotide for Inflammatory Bowel Disease
BL-7040: Oligonucleotide for Inflammatory Bowel Disease December 2012 Forward Looking Statements This presentation contains "forward-looking statements." These statements include words like "may," "expects,"
More informationADVANCED MEDIA TECHNOLOGY
ADVANCED MEDIA TECHNOLOGY For Human ES and ips Cell Research The right medium makes all the difference in ensuring successful ES and ips cell culture. Stemgent offers high-quality, chemically-defined,
More informationOncology Biopharmaceuticals and Preclinical Development: Evolving Regulatory Challenges
The content of this presentation reflects the opinion of the speaker and does not necessarily represent the official position of CDER Oncology Biopharmaceuticals and Preclinical Development: Evolving Regulatory
More informationICH Topic M 3 (R2) Non-Clinical Safety Studies for the Conduct of Human Clinical Trials and Marketing Authorization for Pharmaceuticals.
European Medicines Agency June 2009 CPMP/ICH/286/95 ICH Topic M 3 (R2) Non-Clinical Safety Studies for the Conduct of Human Clinical Trials and Marketing Authorization for Pharmaceuticals Step 4 NOTE FOR
More informationVir B. Singh, PhD
, PhD Vir.Singh@roswellpark.org vir777@gmail.com Current Position Postdoctoral Research Associate at Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, NY, USA since
More informationOERMATAN SULFATE: A NEW CONCEPT IN ANTITHROMBOTIC THERAPY
OERMATAN SULFATE: A NEW CONCEPT IN ANTITHROMBOTIC THERAPY by JOANNE VAN RYN-McKENNA, B.Sc., M.Sc. A Thesis Submitted to the School of Graduate Studies in Partial Fulfilment of the Requirements for the
More informationRespiratory Safety Pharmacology Endpoints in Toxicology
Respiratory Safety Pharmacology Endpoints in Toxicology Simon Authier, DVM, MBA, PhD Director - Safety Pharmacology and Veterinary Science CIToxLAB North America Agenda Introduction Preliminary results
More informationIn vitro mechanistic studies on Metabolism-Dependent Inactivation of Cytochrome P450 Enzymes
In vitro mechanistic studies on Metabolism-Dependent Inactivation of Cytochrome P450 Enzymes Brooke Rock, 2016 DDI Conference, Seattle, WA Acknowledgements: PKDM, Amgen, San rancisco Chi-Chi Peng, John
More informationReceived 22 September 2004; received in revised form 1 December 2004; accepted 13 December 2004
Pain 114 (2005) 212 220 www.elsevier.com/locate/pain Inhibitors of serine/threonine protein phosphatases antagonize the antinociception induced by agonists of a 2 adrenoceptors and GABA B but not k-opioid
More informationThe School of Science and Engineering
The School of Science and Engineering Office: 2000 Percival Stern Hall Phone: (504) 865-5546 Fax: (504) 865-6785 Website: http://cell.tulane.edu Professors Joan W. Bennett, Ph.D., Chicago YiPing Chen,
More informationChapter 18: Regulation of Gene Expression. 1. Gene Regulation in Bacteria 2. Gene Regulation in Eukaryotes 3. Gene Regulation & Cancer
Chapter 18: Regulation of Gene Expression 1. Gene Regulation in Bacteria 2. Gene Regulation in Eukaryotes 3. Gene Regulation & Cancer Gene Regulation Gene regulation refers to all aspects of controlling
More informationCENTER FOR BRAIN EXPERIMENT
CENTER FOR BRAIN EXPERIMENT Section of Brain Structure Associate Professor: ARII, Tatsuo, PhD 1967 Graduated from Tohoku University, Faculty of Science. Completed the doctoral course in Engineering, Nagoya
More informationInVivo Therapeutics. Developing Innovative Products for Spinal Cord Injury
1 Developing Innovative Products for Spinal Cord Injury 2 Forward-Looking Statements Before we begin, we would like to remind everyone that during our presentation, we will be making forward-looking statements
More informationUNIT CELL PROCESSES UNDERLYING TISSUE ENGINEERING AND REGENERATIVE MEDICINE
Massachusetts Institute of Technology Harvard Medical School Brigham and Women s Hospital VA Boston Healthcare System 2.79J/3.96J/20.441/HST522J UNIT CELL PROCESSES UNDERLYING TISSUE ENGINEERING AND REGENERATIVE
More informationGuidelines and criteria for seeking approval to conduct clinical trials in Ayurveda, Siddha and Unani system.
Guidelines and criteria for seeking approval to conduct clinical trials in Ayurveda, Siddha and Unani system. Application for seeking approval of the Central Government for conduct of clinical trial in
More informationCURRENT ISSUE SUBSCRIBE AD LINK 1/10/2010 3:14 PM. SUBSCRIBE Blog with us: BLOGbiotech. (view larger image)
SUBSCRIBE Blog with us: BLOGbiotech CURRENT ISSUE (view larger image) SUBSCRIBE AD LINK HOME Online Exclusives Podcasts Video Blogs Special Reports StockWatch New Products Best of the Web Calendar of Events
More informationНаучно-исследовательский институт онкологии имени Н.Н. Петрова ОТДЕЛ КАНЦЕРОГЕНЕЗА И ОНКОГЕРОНТОЛОГИИ
Научно-исследовательский институт онкологии имени Н.Н. Петрова ОТДЕЛ КАНЦЕРОГЕНЕЗА И ОНКОГЕРОНТОЛОГИИ 197758 Санкт-Петербург, тел. (812) 596-6539; факс (812) 596-8947 Песочный-2, ул. Ленинградская, 68
More informationCOPYRIGHTED MATERIAL. IN THISchapter, we first give definitions of the assay and the bioassay. INTRODUCTION TO ASSAY DEVELOPMENT
CHAPTER1 INTRODUCTION TO ASSAY DEVELOPMENT IN THISchapter, we first give definitions of the assay and the bioassay. Drug discovery and development processes are then reviewed to show the role bioassay
More informationRevised Immunogenicity Guideline: Assays and methods- Presentation of the draft guideline and introduction of the topics for discussion
Revised Immunogenicity Guideline: Assays and methods- Presentation of the draft guideline and introduction of the topics for discussion Robin Thorpe & Meenu Wadhwa Revised Guideline: Differences from original
More informationBUNDESINSTITUT FÜR RISIKOBEWERTUNG
BUNDESINSTITUT FÜR RISIKOBEWERTUNG Mehrfachrückstände von Pflanzenschutzmitteln in Lebensmitteln Teil III Internationale Bewertungskonzepte für Mehrfachrückstände 10.11.2005 Cumulative Risk Assessment:
More informationataluren overview A New Approach to Genetic Disorders
ataluren overview A New Approach to Genetic Disorders Ataluren Scientific Background What is ataluren? Ataluren(formerly PTC124) is the first investigational therapy with the potential to enable the formation
More informationStudent Instructions for using ProQuest Electronic Submission
Student Instructions for using ProQuest Electronic Submission Create your account with ProQuest You can navigate through the software by using the NEXT button at the bottom of each page, or by using the
More informationUse of animal models with disease in Safety Pharmacology or You don t give drugs to normal people!
Use of animal models with disease in Safety Pharmacology or You don t give drugs to normal people! Robert L. Hamlin, DVM, PhD, DACVIM (Cardiology/Internal Medicine), Stanton Youngberg Professor of Veterinary
More informationProteomics. Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Limitations: Examples
Proteomics Areas of Application for Proteomics Most Commonly Used Proteomics Techniques: Antibody arrays Protein activity arrays 2-D gels ICAT technology SELDI Limitations: protein sources surfaces and
More informationANTIBODY IMMUNOGENICITY
ANTIBODY IMMUNOGENICITY Tolerance Classical Immunity To aggegated Antibody Chiller and Weigle, PNAS, 65:551, 1970; Benjamin and Waldmann, et al, J Exp Med, 163:1539, 1986) THE IMMUNOGENICITY PROBLEM WITH
More informationThe Road to Functional Bioanalysis: Development and Validation of a Cell-Based Assay for Neutralizing Anti-Drug Antibody Analysis
The Road to Functional Bioanalysis: Development and Validation of a Cell-Based Assay for Neutralizing Anti-Drug Antibody Analysis Christelle Pythoud, PhD 2 nd EBF YSS, Barcelona Spain Celerion Switzerland
More informationDifferential Gene Expression
Biology 4361 Developmental Biology Differential Gene Expression June 19, 2008 Differential Gene Expression Overview Chromatin structure Gene anatomy RNA processing and protein production Initiating transcription:
More informationTHE MEASUREMENT OF ANALGESIC PROPERTIES OF INHALATION DRUGS GIVEN IN SUB-ANAESTHETIC CONCENTRATIONS
Brit. J. Pharmacol. (1964), 22, 590-596. THE MEASUREMENT OF ANALGESIC PROPERTIES OF INHALATION DRUGS GIVEN IN SUB-ANAESTHETIC CONCENTRATIONS BY M. J. NEAL AND J. M. ROBSON From the Department of Pharmacology,
More informationANTI NOCICEPTIVE ACTIVITY OF ARGENTUM NITRICUM, STAPHYSAGRIA, IGNATIA AMARA IN MICE IN COMPARISON WITH ACETYL SALICYLIC ACID (ASPIRIN)
IJPSR (2015), Vol. 6, Issue 1 (Research Article) Received on 01 May, 2014; received in revised form, 16 July, 2014; accepted, 20 September, 2014; published 01 January, 2015 ANTI NOCICEPTIVE ACTIVITY OF
More informationPHARMACOLOGY. Dr Paul Spiers, Dept of Pharmacology & Therapeutics Tel ,
PHARMACOLOGY COURSE COORDINATOR: Dr Paul Spiers, Dept of Pharmacology & Therapeutics Tel 896 2146, spiersj@tcd.ie ECTS Value 10 Credits Contact Hours 46 hrs lectures 2 tutorials 3 hr 2 computer sessions
More informationIMPURITIES IN NEW DRUG PRODUCTS
INTERNATIONAL CONFERENCE ON HARMONISATION OF TECHNICAL REQUIREMENTS FOR REGISTRATION OF PHARMACEUTICALS FOR HUMAN USE ICH HARMONISED TRIPARTITE GUIDELINE IMPURITIES IN NEW DRUG PRODUCTS Recommended for
More informationPreclinical Drug Development
Preclinical Drug Development A guidance prepared by From a 2004 NIH Summit Workshop: A major reason for the tremendous cost of drug development is the high rate of drug candidate failure during clinical
More informationStrategies to Improve Drug Tolerance in Nab Assays
Strategies to Improve Drug Tolerance in Nab Assays Steven J Swanson, PhD Senior Vice President, Research ImmunoCellular Therapeutics Ltd steven.swanson@imuc.com AAPS National Biotech Conference June 2015
More informationPatentability/Literature Research
Patentability/Literature Research The probiotic-based solution to combat cholera as presented here comprises of two major innovative components: (1) the metabolite-dependent pathogen inhibition by a probiotic
More informationStem Cells: The Revolution of Blood Transfusion. By Srinand Sundaram. Grade awarded: Pass with Merit
Stem Cells: The Revolution of Blood Transfusion By Srinand Sundaram Grade awarded: Pass with Merit Research Paper Based on Pathology Lectures At Medlink, December 2014 Abstract For people around the world
More informationCentral Delivery of Dicer-substrate sirna: A Direct Application for Pain Research
original article Central Delivery of Dicer-substrate sirna: A Direct Application for Pain Research Louis Doré-Savard 1, Geneviève Roussy 1, Marc-André Dansereau 1, Michael A Collingwood 2, Kim A Lennox
More informationMitragynine/corynantheidine pseudoindoxyls as opioid analgesics with mu agonism and delta antagonism which do not recruit #-arrestin-2
Article Subscriber access provided by University of Idaho Library Mitragynine/corynantheidine pseudoindoxyls as opioid analgesics with mu agonism and delta antagonism which do not recruit #-arrestin- András
More information