Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 Microbiology Department Bedford Hospital South Wing Kempston Road Bedford MK42 9DJ Contact: Helen Gough Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only DETAIL OF ACCREDITATION Routine samples: Cellular and fluid material contained on swabs, catheter tips and intrauterine contraceptive devices Tissues Blood routine & blood samples listed above Corneal scrapes Eye (specimens from) Tissues samples listed above diagnosis Detection, identification and quantification of: White blood cells Red blood cells Bacterial cells Yeast cells and fungal hyphae following current Public Health England (PHE) Standards for Microbiological Investigations (SMIs) Manual inoculation of selected culture media using in-house BED-SOP-151 Liquid culture, using in-house BED-SOP-35 BED-SOP-119 instructions using BacTec FX BED-SOP-25 BED-SOP-50 BED-SOP-51 BED-SOP-68 BED-SOP-152 BED-SOP-153 BED-SOP-837 Staining (inc. Gram) and microscopic BED SOP-701 Assessment Manager: MS2 Page 1 of 5
2 urine samples Respiratory samples: Bronchoalveolar lavage Bronchial aspirate, brushings & washings Endotrachael tube specimen Pleural fluid Sputum respiratory samples listed above Cultures of faecal bacterial pathogens isolated in-house Detection, identification and quantification of: White blood cells Red blood cells Squamous epithelial cells Bacterial cells (inc. Legionella spp.) Isolation of bacterial pathogens: Campylobacter spp. Clostridium difficile Escherichia coli O157 Salmonella sp. Shigella spp. Vibrio spp. Yersinia spp. Image capture and recognition using BED-SOP-820 instructions using A.Menarini Diagnositics SediMAX system using BED-SOP-18 BED-SOP-150 using BED-SOP-17 BED-SOP-17 using BED-SOP-11 BED-SOP-11 Assessment Manager: MS2 Page 2 of 5
3 Bacterial cultures isolated in-house from all samples listed above Sputum Tissue Antimicrobial susceptibility testing of clinically significant bacteria Detection of Legionella pneumophilia serotype 1 antigen Detection of Streptococcus pneumoniae soluble antigen Isolation and presumptive identification of Acid-alcohol fast bacilli (inc. Mycobacterium spp.) Disc diffusion and gradient minimum inhibitory concentration determination using the latest European Committee on Antimicrobial Susceptibility Testing (EUCAST) methodology an in-house BED-SOP-91 assay using in house BED-SOP-88 instructions using Binax Now assay using in house BED-SOP-88 instructions using Binax Now Manual culture using Löwenstein- Jensen media and/or automated liquid culture, followed by auramine phenol staining and incident-light fluorescence microscopic BED-SOP-58 BED-SOP-121 instructions using BacTec MGIT 960 Assessment Manager: MS2 Page 3 of 5
4 Sellotape slide Perianal swab Bile Duodenal aspirates Jejunal aspirates Female genital tract swabs Female genital tract swabs Detection of ova, cysts and parasites (OCP) Detection of Cryptosporidium oocysts Detection of Enterobious vermicularis larvae and ova Detection of Schistosoma haematobium ova Detection of OCP including: Strongyloides stercoralis larvae, Giardia lamblia trophozoites Cryptosporidium oocysts Cyclospora cayetanesis oocysts Cystoisospora belli oocysts Detection of Trichomonas vaginalis ova Microscopic diagnosis of bacterial vaginosis Detection of Rotavirus and Adenovirus antigens Unstained and iodine stained direct microscopic examination and/or Formol-ether faecal concentration and microscopic examination using in-house Auramine phenol staining and incident-light fluorescence microscopic examination using inhouse Microscopic examination using inhouse Microscopic examination using inhouse Auramine phenol staining and incident-light fluorescence, Ziehl- Neelsen, iodine staining as appropriate and microscopic Culture and microscopic examination using in-house BED-SOP-25 Gram stained microscopic examination against Hay s criteria using in-house BED-SOP-876 assay using in-house BED-SOP-94 Assessment Manager: MS2 Page 4 of 5
5 Nasopharyngeal aspirates & swabs Nasal washes Endocervical swabs Male urethral swabs Vaginal swabs Detection of Clostridium difficile glutamate dehydrogenase and Toxins A & B Detection of Helicobacter pylori antigen Detection of Respiratory Syncytial Virus fusion protein antigen Detection of Chlamydia trachomatis and Neisseria gonorrhoeae ribosomal RNA Miscellaneous assay using in-house BED-SOP-108 instructions using C diff Quick Chek Complete Automated sandwich enzyme immunoassay using in-house BED-SOP-930 instructions using Dynex DS2 and Amplified IDEIA TM Hp StAR TM assay using in-house BED-SOP-89 instructions using Binax NOW Nucleic acid amplification technique (NAAT) using in-house BED-SOP-856 instructions using: Hologic Gen-Probe Panther system and APTIMA Combo CT/GC assay Occult blood Qualitative immunochemical testing using in-house BED-SOP-35 instructions using hema-screen SPECIFIC END Assessment Manager: MS2 Page 5 of 5
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
Department of Microbiology 3 rd Floor Darent Valley Hospital Darenth Wood Road Dartford DA2 8DA United Kingdom Contact: Mr Kurt Djemal Tel: +44 (0) 1322 428733 E-Mail: k.djemal@nhs.net Website: http://www.dvh.nhs.uk/
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