Value Correct Answer Feedback. Student Response. A. Dicer enzyme. complex. C. the Dicer-RISC complex D. none of the above
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1 1 RNA mediated interference is a post-transcriptional gene silencing mechanism Which component of the RNAi pathway have been implicated in cleavage of the target mrna? A Dicer enzyme B the RISC-siRNA complex C the Dicer-RISC complex D none of the above E all of the above 0% 2 XIST is a gene product that affects gene expression off the presumptive inactive X by what mechanism? A It coats the X chromosome and then promotes the formation of heterochromatin B it is a non-coding RNA that induces the formation of the Barr Body which is the inactive X in female cells C it will eventually cause changes in the acetylation levels of histone
2 tails on the inactive X so that they become hyperacetylated D a and b E a, b and c 0% 3 The MAT locus allows yeast to switch mating type through a very complex mechanism However, it has informed us a great deal about what aspects of gene expression typical to all organisms? A higher order changes in chromatin affect transcriptional efficiency B that general transcription factors must first bind directly to histone tails and only then can they interact with their cognate binding sites C that multiple proteins are involved in this silencing mechanism D a and b E a and c 100% Score: 1/1
3 4 Which of these aspects of microarray analysis is not true? A Analysis of genes expressed in similar patterns has helped to understand how genes are related based on their expression pattern B microarrays allow researchers to group genes according to how they respond to various environmental stimuli C Microarrays accurately measure the half life and the abundance of each mrna in the sample D microarrays represent an effective means of examining gene expression of all genes at a given time or under a specific condition 0% E none of the above are false
4 5 Targeting constructs or disruption constructs require significant knowledge of the sequence of the target genewhy? A The selectable marker needs these sequences to correctly express the protein B The flanking sequence around the gene will enhance the expression of an antibiotic resistance gene C DNA sequence flanking the target gene must be incorporated into the targeting construct to facilitate accurate recombination at the desired target site 100% D a and b E a, b and c Score: 1/1 6 You have isolated and identified two proteins from a nuclear extract To see if the two proteins might interact together you decided to use a yeast two-hybrid analysis To do this you have constructed a bait and a prey vector When you introduce the bait construct (alone) into a yeast strain that cannot grow on medium lacking histidine unless transcription
5 of the HIS reporter/selectable marker gene is activated, you find that the yeast grows fine (on medium lacking histidine) What might this observation tell you about the function of your protein that you introduced into the bait vector? A That the protein used in the bait construct may activate transcription if brought to a promoter and might therefore be a transcriptional activator B Sequence and structural relationships that indicate how genes arose/evolved ie gene duplication C Your bait protein can interact with your prey protein since the system is based on the modular nature of transcription factors D That the DNA binding specificity of your protein is very non-specific 0% E That your prey protein does not interact with the bait protein
6 7 Based on the observation in Q6, the yeast two-hybrid system would not be a useful means to test whether these two proteins interact- why not? A Because you already know the function of the protein in the bait construct B The activity of the yeast strain seems far too nonspecific so it would be best to simply change the yeast strain C The ability of your bait protein to interact with the DNA is too strong and it outcompetes the GAL-4 DNA binding domain 0% D The protein in the bait construct autoactivates transcription of the reporter/marker and therefore its ability to interact with the prey cannot be determined based on activating the transcription of
7 HIS reporter/selectable marker gene E It will work-why forget about it? 8 Which of the following is an example of reverse genetic analysis? A RNAi B gene disruption in yeast C gene targeting in mice D a and b only E a, b and c 100% Score: 1/1 9 The RNAi and the microrna (mirna) pathways share many components and characteristics What unique feature distinguishes the microrna pathway from RNAi? A only the RNAi pathway is initiated by dsrna molecules B the mirna pathway does not require Dicer
8 C the mirna pathway does not require RISC D mirnas have 100% complementarity with their mrna targets to block translation 0% E none of the above 1 0 What key mammalian protein that is part of a multiprotein complex is required to recognize epigenetic changes (methylation of CpG islands in the DNA) and initiate changes in the chromatin to silence gene expression in the region A msin3 B Rpd3 0% C RAP1 D Sir2 E Sir3
9 1 Quiz 6 Q RNA mediated interference is a post-transcriptional gene silencing mechanism, but components of the RNAi pathway have been implicated in affecting gene expression at the transcriptional level as well How can it affect transcription of genes? A antisense RNAs bind to the transcripts being expressed from this region and initiate the RNAi process B Dicer-mediated cleavage products feed back onto the DNA to change chromatin configuration C when bound to a chromosomal region dsrnas somehow recruit methyltransferases to modify histone tails in their proximity D a and b E b and c 100% Score: 1/1 2 Quiz 6 Q Xist is a gene product that affects gene expression off the presumptive inactive X by what mechanism? A It coats the X chromosome in
10 cis (from which it is transcribed) and then promotes the formation of heterochromatin B it is a noncoding RNA that induces the formation of the Barr Body which corresponds to the inactive X in female cells C it will eventually cause changes in the acetylation levels of histone tails on the inactive X so that they become hypoacetylated D a and b only 0% E a, b and c 3 Quiz 6 Q The MAT locus allows yeast to switch mating type through a very complex mechanism However, it has informed us a great deal about what aspects of gene expression typical to all organisms? A higher order changes in chromatin
11 affect transcriptional efficiency B that general transcription factors must first bind directly to histone tails and only then can they interact with their cognate binding sites C that proteins involved in this mechanism also silence telomeric regions D a and b only 0% E a and c 4 Quiz 6 Q What aspect of microarray analysis is not true? A The levels of the mrnas are quantitative and indicate precisely how many moles of a given mrna are present in the sample B microarrays allow 100%
12 researchers to group genes according to how they respond to various environmental stimuli C Cluster analysis has helped to understand how genes are related based on their expression pattern D microarrays represent an effective means of examining gene expression of all genes at a given time or under a specific condition E none of the above are false Score: 1/1 5 Quiz 6 Q Targeting constructs or disruption constructs require significant knowledge of the sequence of the target genewhy? A DNA sequence flanking the 100%
13 target gene must be incorporated into the targeting construct to facilitate accurate recombination at the desired target site B The flanking sequence around the gene will enhance the expression of an antibiotic resistance gene C The selectable marker needs these sequences to correctly express the protein D a and b only E a, b and c Score: 1/1 6 Quiz 6 Q Some people think that it is useless to look at any other genome now that the human genome is fully sequenced What information could be gained by comparing the human genome with other genomes of closely and/or distantly related species? A Syntenic relationships that might 0%
14 reflect conserved regulatory mechanisms B Sequence and structural relationships that indicate how genes arose/evolved ie gene duplication C Non-conserved nucleotide sequence probably indicates regions that are under high selective pressure to change D a and b only E a, b and c 7 Quiz 6 Q Name an important limitation in the use of yeast two hybrid analysis to examine protein:protein interactions
15 A strains containing baits that autoactivate cannot be used, therefore excluding most transcriptional activators 100% B Yeast does not normally express all the proteins that investigators might be interested in C lacz or His reporter genes that are introduced into yeast can independently confer growth on selective media D the DNA binding sites of your protein of interest are not necessarily conserved in the yeast genome E c and d only Score: 1/1 8 Quiz 6 Q What evidence suggests that components of the RNAi pathway are involved in silencing centromeric DNA?
16 A dsrna have been identified that correspond to the sequences in the centromeric DNA sequence B Dicer is required to maintain the silencing of this region C RISC components are involved in silencing the centromeric DNA D a and b only E a, b and c 100% Score: 1/1 9 Quiz 6 Q The RNAi and the microrna (mirna) pathways share many components and characteristics What feature(s) typical of the microrna pathway distinguish(es) it from RNAi? A only the RNAi pathway is initiated by dsrna molecules B the mirna pathway does
17 not require Dicer C the mirna pathway does not require RISC D mirnas have imperfect complementarity with their mrna targets which results in translational inhibition E none of the above Score: 1/1 100% 1 0 Quiz 6 Q During the generation of knock-out mice a gene is targeted through the use of homologous recombination How can you ensure that your targeting construct did not simply integrate randomly anywhere in the genome? A the dominant selectable marker (usually neomycin resistance) will ensure that this event was specific B two rounds of drug selection; first to select for all integrants followed by
18 negative selection using a drug that selects against a gene outside the homologous region (tk) C because the ES cells that you used possess a brown coat colour gene D a and b E a, b and c 0%
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