Visualize cell junctions and cellular adhesion
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1 Cellular Structure Visualize cell junctions and cellular adhesion Antibodies to study specific protein localization and cellular processes Cellular adhesion is essential for providing physical support and positional information in multicellular organisms. Such functions are mediated by adhesion receptor recognition of extracellular matrix (or cellular receptors on other cells) and through receptor control of cytoskeletal architecture and signaling cascades (Figure 1). Adhesion receptor signal transduction also integrates with signals from other receptor systems to regulate cell movement, differentiation, proliferation, and survival. Deregulation of adhesion contributes to disease progression, either by disrupting the normal docking and movement of cells, which alters regulatory signaling, or by promoting inappropriate temporal and spatial adhesion. Cell junctions influence both the structure and behavior of adjacent cells by regulating how these cells interact with each other and with the extracellular matrix. Understanding cell junctions and their role in triggering cellular adhesion signaling pathways is fundamental to understanding disease processes, and for developing treatments that target tumor plasticity and angiogenesis. Unfortunately, it is difficult to discretely visualize cellular junctions and their associated signaling cascades, due in part to challenges in finding primary antibodies that recognize their targets with high specificity and affinity. Now you can get highly specific antibodies that allow you to visualize the proteins and protein modifications that are critical to your research (Figure 2). Zymed antibodies for the study of cell junctions, and BioSource antibodies for cell adhesion research (both available from Invitrogen), produce highly specific identification of target proteins. You ll detect only the proteins you want, with low background. Invitrogen s cell junction and cell adhesion antibodies are widely used and widely cited throughout the literature, demonstrating their reliability. Figure 1 Detection of Pyk2 [py402] in NMµMG (mouse mammary) cells stimulated with 20 ng/ml TGFβ for 36 hours. Pyk2 [py402] was identified and visualized using BioSource Pyk2 [py402] specific antibody directly conjugated to Alexa Fluor 488 dye (Invitrogen Cat. no A1). Nuclei are stained with DAPI (blue). The inset image shows cells not treated with TGFβ (control), which lack Pyk2 phosphorylation at site 402. Figure 2 Indirect immunofluorescence staining of adherens junctions between highly confluent HeLa cells using Zymed mouse anti α-catenin (Invitrogen Cat. no ). α-catenin (green) was visualized using goat anti mouse IgG Alexa Fluor 488 (Invitrogen Cat. no. A11029). Slides were mounted in ProLong Gold Antifade Reagent (Invitrogen Cat. no. P36931), which contains DAPI for nuclear counterstaining (blue). F-actin (red) was visualized using Alexa Fluor 635 phalloidin (Invitrogen Cat. no. A34054). Get the whole picture. To learn more about Invitrogen s antibodies for the study of cell junctions and cellular adhesion, visit Invitrogen Corporation. All rights reserved. These products may be covered by one or more Limited Use Label Licenses (see Invitrogen catalog or By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated. A r1 US
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