Material Safety Data Sheet

Transcription

1 Conforms to ANSI Z Standard (United States) 1. Product name Material uses Material Safety Data Sheet Product and company identification Supplier/Manufacturer Stratagene PfuTurbo DNA Polymerase, Catalog # Part No. (Chemical Kit) Part No. PfuTurbo DNA Polymerase Validation date 08/5/2010 In case of emergency 2. Emergency overview Stratagene PfuTurbo DNA Polymerase, Catalog # Analytical reagent. PfuTurbo DNA Polymerase Agilent Technologies, Inc State Highway 71 West Cedar Creek, TX Hazards identification Physical state PfuTurbo DNA Polymerase Liquid. Liquid. Routes of entry Potential acute health effects PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase 0.4 ml 4 ml (4 x 1 ml) Odor PfuTurbo DNA Polymerase OSHA/HCS status PfuTurbo DNA Polymerase This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR ). Signal word Hazard statements Precautions This material is considered hazardous by the OSHA Hazard Communication Standard (29 CFR ). DANGER! MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. CAUSES RESPIRATORY TRACT, DIGESTIVE TRACT, EYE AND SKIN BURNS. Avoid breathing vapor or mist. Avoid contact with eyes. Avoid prolonged or repeated contact with skin. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. Do not breathe vapor or mist. Do not ingest. Do not get in eyes or on skin or clothing. Use only with adequate ventilation. Keep container tightly closed and sealed until ready for use. Wash thoroughly after handling. Date of issue 08/5/ /11

2 Stratagene PfuTurbo DNA Polymerase, Catalog # Inhalation Ingestion Skin Hazards identification PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase Eyes PfuTurbo DNA Polymerase Potential chronic health effects Slightly irritating to the respiratory system. Corrosive to the respiratory system. Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure. No known significant effects or critical hazards. Corrosive to the digestive tract. Causes burns. Slightly irritating to the skin. Corrosive to the skin. Causes burns. Slightly irritating to the eyes. Corrosive to eyes. Causes burns. Chronic effects PfuTurbo DNA Polymerase Contains material that may cause target organ damage, based on animal data. No known significant effects or critical hazards. Carcinogenicity PfuTurbo DNA Polymerase No known significant effects or critical hazards. No known significant effects or critical hazards. Mutagenicity PfuTurbo DNA Polymerase No known significant effects or critical hazards. No known significant effects or critical hazards. Teratogenicity PfuTurbo DNA Polymerase No known significant effects or critical hazards. No known significant effects or critical hazards. Developmental effects PfuTurbo DNA Polymerase No known significant effects or critical hazards. No known significant effects or critical hazards. Fertility effects PfuTurbo DNA Polymerase No known significant effects or critical hazards. No known significant effects or critical hazards. Target organs PfuTurbo DNA Polymerase Contains material which may cause damage to the following organs kidneys, upper respiratory tract, skin, eye, lens or cornea. Over-exposure signs/symptoms Inhalation PfuTurbo DNA Polymerase Adverse symptoms may include the following respiratory tract irritation coughing Ingestion PfuTurbo DNA Polymerase No specific data. Adverse symptoms may include the following stomach pains Skin PfuTurbo DNA Polymerase Adverse symptoms may include the following irritation redness Adverse symptoms may include the following respiratory tract irritation coughing Adverse symptoms may include the following pain or irritation redness blistering may occur Date of issue 08/5/ /11

3 Stratagene PfuTurbo DNA Polymerase, Catalog # Hazards identification Eyes PfuTurbo DNA Polymerase Adverse symptoms may include the following irritation watering redness Medical conditions aggravated by overexposure See toxicological information (section 11) 3. PfuTurbo DNA Polymerase Composition/information on ingredients Adverse symptoms may include the following pain watering redness Pre-existing disorders involving any target organs mentioned in this MSDS as being at risk may be aggravated by over-exposure to this product. None known. Name CAS number % PfuTurbo DNA Polymerase Glycerol Amino-2-(hydroxymethyl)propane-1,3-diol hydrochloride Dodecyldimethyl(3-sulphonatopropyl)ammonium Ammonium sulphate There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. 4. Eye contact Skin contact Inhalation First aid measures PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase Date of issue 08/5/ Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. Check for and remove any contact lenses. Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical attention immediately. In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Clean shoes thoroughly before reuse. Get medical attention immediately. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Move exposed person to fresh air. If not breathing, if breathing is irregular or if respiratory 3/11

4 Stratagene PfuTurbo DNA Polymerase, Catalog # Ingestion First aid measures Notes to physician PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase arrest occurs, provide artificial respiration or oxygen by trained personnel. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Wash out mouth with water. Do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Get medical attention immediately. Protection of first-aiders PfuTurbo DNA Polymerase No action shall be taken involving any personal risk or without suitable training. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. 5. Fire-fighting measures Flammability of the product Extinguishing media Suitable PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase Not suitable PfuTurbo DNA Polymerase Special exposure hazards PfuTurbo DNA Polymerase No action shall be taken involving any personal risk or without suitable training. If it is suspected that fumes are still present, the rescuer should wear an appropriate mask or self-contained breathing apparatus. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. Wash contaminated clothing thoroughly with water before removing it, or wear gloves. No specific treatment. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. In case of inhalation of decomposition products in a fire, symptoms may be delayed. The exposed person may need to be kept under medical surveillance for 48 hours. In a fire or if heated, a pressure increase will occur and the container may burst. In a fire or if heated, a pressure increase will occur and the container may burst. Use an extinguishing agent suitable for the surrounding fire. Use an extinguishing agent suitable for the surrounding fire. None known. None known. No action shall be taken involving any personal risk or without suitable training. No action shall be taken involving any personal risk or without suitable training. Date of issue 08/5/ /11

5 Stratagene PfuTurbo DNA Polymerase, Catalog # Fire-fighting measures Hazardous thermal decomposition products Special protective equipment for fire-fighters 6. PfuTurbo DNA Polymerase Decomposition products may include the following materials carbon dioxide carbon monoxide Accidental release measures Personal precautions Environmental precautions Methods for cleaning up Decomposition products may include the following materials carbon dioxide carbon monoxide nitrogen oxides sulfur oxides halogenated compounds Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. PfuTurbo DNA Polymerase No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Avoid breathing vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilled material. Do not breathe vapor or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment (see section 8). PfuTurbo DNA Polymerase Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). PfuTurbo DNA Polymerase Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if watersoluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Date of issue 08/5/ /11

6 Stratagene PfuTurbo DNA Polymerase, Catalog # Handling and storage Handling PfuTurbo DNA Polymerase Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Put on appropriate personal protective equipment (see section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Do not get in eyes or on skin or clothing. Do not breathe vapor or mist. Do not ingest. Use only with adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Storage PfuTurbo DNA Polymerase Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabeled containers. Use appropriate containment to avoid environmental contamination. Date of issue 08/5/ /11

7 Stratagene PfuTurbo DNA Polymerase, Catalog # Ingredient Exposure controls/personal protection PfuTurbo DNA Polymerase Glycerol ACGIH TLV (United States, 1/2009). TWA 10 mg/m³ 8 hour(s). Form Mist OSHA PEL (United States, 11/2006). TWA 5 mg/m³ 8 hour(s). Form Respirable fraction TWA 15 mg/m³ 8 hour(s). Form Total dust OSHA PEL 1989 (United States, 3/1989). TWA 5 mg/m³ 8 hour(s). Form Respirable fraction TWA 10 mg/m³ 8 hour(s). Form Total dust Recommended monitoring procedures Engineering measures Hygiene measures Personal protection Respiratory Hands Eyes Skin Environmental exposure controls Other protection 9. If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Use only with adequate ventilation. If user operations generate dust, fumes, gas, vapor or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists or dusts. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. Exposure limits Physical and chemical properties Physical state PfuTurbo DNA Polymerase Liquid. Liquid. Flash point PfuTurbo DNA Polymerase Auto-ignition temperature PfuTurbo DNA Polymerase Flammable limits PfuTurbo DNA Polymerase Date of issue 08/5/ /11

8 Stratagene PfuTurbo DNA Polymerase, Catalog # Physical and chemical properties Color PfuTurbo DNA Polymerase Odor ph PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase Boiling/condensation point Melting/freezing point Specific gravity Vapor pressure Vapor density Volatility Odor threshold Evaporation rate Viscosity Solubility 10. Stability and reactivity Chemical stability Conditions to avoid Materials to avoid PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase The product is stable. PfuTurbo DNA Polymerase PfuTurbo DNA Polymerase Soluble in the following materials cold water and hot water. Easily soluble in the following materials cold water and hot water. No specific data. No specific data. Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Slightly reactive or incompatible with the following materials organic materials. Reactive or incompatible with the following materials oxidizing materials, reducing materials, metals, acids and alkalis. Date of issue 08/5/ /11

9 Stratagene PfuTurbo DNA Polymerase, Catalog # Stability and reactivity Hazardous decomposition products Possibility of hazardous reactions 11. PfuTurbo DNA Polymerase Toxicological information Acute toxicity Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous reactions will not occur. Product/ingredient name Result Species Dose Exposure PfuTurbo DNA Polymerase Glycerol LD50 Oral Rat mg/kg - Ammonium sulphate LD50 Oral Rat 2840 mg/kg - Chronic toxicity Irritation/Corrosion Sensitizer Carcinogenicity Mutagenicity Teratogenicity Reproductive toxicity Other adverse symptoms PfuTurbo DNA Polymerase 12. Ecotoxicity Ecological information Aquatic ecotoxicity Product/ingredient name This material is toxic to aquatic life. Result Species PfuTurbo DNA Polymerase Glycerol Acute LC50 54 to 57 ml/l Fresh water Fish - Oncorhynchus mykiss g Exposure 96 hours 10X Cloned Pfu Reaction Buffer Ammonium sulphate Acute LC to ug/l Fresh Daphnia - Daphnia magna - water Young - <=24 hours Acute LC50 68 ug/l Fresh water Fish - Oncorhynchus gorbuscha - ALEVIN 48 hours 96 hours Date of issue 08/5/ /11

10 Stratagene PfuTurbo DNA Polymerase, Catalog # Ecological information Octanol/water partition coefficient Other adverse effects 13. Disposal considerations Waste disposal PfuTurbo DNA Polymerase No known significant effects or critical hazards. The generation of waste should be avoided or minimized wherever possible. Empty containers or liners may retain some product residues. This material and its container must be disposed of in a safe way. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any byproducts should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains and sewers. Disposal should be in accordance with applicable regional, national and local laws and regulations. Local regulations may be more stringent than regional or national requirements. The information presented below only applies to the material as supplied. The identification based on characteristic(s) or listing may not apply if the material has been used or otherwise contaminated. It is the responsibility of the waste generator to determine the toxicity and physical properties of the material generated to determine the proper waste identification and disposal methods in compliance with applicable regulations. Refer to Section 7 HANDLING AND STORAGE and Section 8 EXPOSURE CONTROLS/PERSONAL PROTECTION for additional handling information and protection of employees. 14. Transport information Regulatory information DOT / IMDG / IATA / 15. HCS Classification Not regulated. Regulatory information U.S. Federal regulations Clean Air Act Section 112(b) Hazardous Air Pollutants (HAPs) Clean Air Act Section 602 Class I Substances Clean Air Act Section 602 Class II Substances PfuTurbo DNA Polymerase Target organ effects Corrosive material TSCA 8(a) IUR Glycerol; Water; Albumins, blood serum United States inventory (TSCA 8b) At least one component is not listed. SARA 302/304/311/312 extremely hazardous substances No products were found. SARA 302/304 emergency planning and notification No products were found. SARA 302/304/311/312 hazardous chemicals Glycerol; Ammonium sulphate SARA 311/312 MSDS distribution - chemical inventory - hazard identification Glycerol Immediate (acute) health hazard, Delayed (chronic) health hazard; Ammonium sulphate Immediate (acute) health hazard Clean Water Act (CWA) 307 No products were found. Clean Water Act (CWA) 311 Edetic acid Clean Air Act (CAA) 112 accidental release prevention No products were found. Clean Air Act (CAA) 112 regulated flammable substances No products were found. Clean Air Act (CAA) 112 regulated toxic substances No products were found. Not listed Not listed Not listed Date of issue 08/5/ /11

11 Stratagene PfuTurbo DNA Polymerase, Catalog # SARA 313 Regulatory information DEA List I Chemicals (Precursor Chemicals) DEA List II Chemicals (Essential Chemicals) Form R - Reporting requirements Supplier notification Massachusetts The following components are listed AMMONIUM SULFATE; GLYCERINE MIST New York None of the components are listed. California Prop. 65 No products were found. Not listed Not listed Product name CAS number Concentration Ammonium sulphate Ammonium sulphate SARA 313 notifications must not be detached from the MSDS and any copying and redistribution of the MSDS shall include copying and redistribution of the notice attached to copies of the MSDS subsequently redistributed. State regulations New Jersey The following components are listed GLYCERIN; 1,2,3-PROPANETRIOL Pennsylvania The following components are listed SULFURIC ACID DIAMMONIUM SALT; 1,2,3- PROPANETRIOL 16. Other information Label requirements Date of issue Date of previous issue Version Notice to reader PfuTurbo DNA Polymerase 08/5/2010 No previous validation. 1 Indicates information that has changed from previously issued version. MAY CAUSE RESPIRATORY TRACT, EYE AND SKIN IRRITATION. CONTAINS MATERIAL THAT MAY CAUSE TARGET ORGAN DAMAGE, BASED ON ANIMAL DATA. CAUSES RESPIRATORY TRACT, DIGESTIVE TRACT, EYE AND SKIN BURNS. Disclaimer The information contained in this document is based on Agilent s state of knowledge at the time of preparation. No warranty as to its accurateness, completeness or suitability for a particular purpose is expressed or implied. Date of issue 08/5/ /11