Bacteria and other microbes have particular requirements for growth When they reside in and on our bodies or in the environment, they harvest their
|
|
- Blanche Stokes
- 5 years ago
- Views:
Transcription
1 Bacteria and other microbes have particular requirements for growth When they reside in and on our bodies or in the environment, they harvest their food from us or from the environment When we grow bacteria in lab, we are essentially creating a captive environment for bacteria like a bacteria zoo. So we must provide the bacteria we grow in lab with all of the materials that they need to grow
2 In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any type of bacteria and the others are more selective and only grow specific types of bacteria
3 There are generalized media, like (Nutrient agar)that will grow many different types of microbes. This media is the type most often used to culture bacteria
4 culture medium that allows the growth types of organisms, while inhibiting the growth of other organisms Example: EMB (Eosin Methylene Blue) dyes inhibit Gram (+) bacteria selects for Gram (-) bacteria
5 culture medium that includes ingredients, such as chemical indicators, that produce observable differences between species of bacteria Differentiates between different organisms growing on the same plate Example: Blood agar
6 This media is differential because: Certain bacteria produce enzymes (hemolysins ) that act on the red cells to produce either: Beta hemolysis: Enzymes lyse the blood cells completely, producing a clear area around the colony. Alpha hemolysis: Incomplete hemolysis produces a greenish discoloration around the colony Gamma hemolysis: No effect on the red cells.
7 There are also (selective) and (differential)media
8 MacConkey s is both a selective & differential media. 1. MacConkey s is selective media because it inhibits the growth of some organisms [Gram positive bacteria]. 2. MacConkey s is differential media - lactose fermenters bacteria will grow in red colonies while non-lactose fermenters will be colorless and clear. So if there are colonies of bacteria growing on MacConkey s, it s understood that they are Gram- If those colonies are colorless, they are not lactose fermenters If the colonies have a pinkish appearance, they are lactose fermenters
9
10 MacConkey Agar left: no lactose fermentation right: lactose fermentation
11 FORMS OF CULTURE MEDIA broth liquid medium most common growth media for microorganisms are ( nutrient broths ( liquid nutrient medium
12 Solid media commonly contain 1.5% agar per weight to solidify the liquid media. After sterilization, the media is poured into sterile Petrie plates. Agar is liquefies at 100 C and solidifies at 40 C
13 General Media: Nutrient Broth and Nutrient Agar
14 or After autoclaving the media(in tube) for 20 minutes, the tubes are placed in a slanted position to allow the agar to solidify. These tubes are called slants
15 Slant tubes: are tubes containing a nutrient medium plus a solidifying agent, (agar-agar. The medium has been allowed to solidify at an angle in order to get a flat inoculating surface Agar Plate: are sterile petri plates that are filled with a melted sterile agar medium Microorganisms grow on the surface of agar plates and slants
16 140 Micro Lab 4: Isolation of microbes from different environments
17 Isolation of micro organisms from various environments
18 1. Isolation separating one species from another. 2. Inoculation introduction of a sample into a container of media to produce a culture of observable growth. 3. Incubation conditions that allow growth eg., temperature,humidity etc.. 4. Inspection. 5. Identification.
19 Environmental Sample Normal Flora Samples Surface samples are normally taken using sterile swabs Applying oral sample to surface of agar
20
21 Petri plates with media Soil Rotten fruit Yoghurt Mouth swab etc Incubator
22 Bacteria Fungi
23 Isolation of Bacteria Yoghurt Put a drop of diluted yoghurt on the solid media plate of bacteria. Incubate at 37 C for 1 day. Mouth Take some saliva with the help of a cotton swab. Inoculate it on the media and incubate. Hand Touch the surface of the solid media plate. Incubate 37 C for 1 day..
24 Agar plates are stored upside down to prevent condensation.
25 These plates of bacteria will be incubated at 37 C for 24 hours and then stored at refrigerator until next week when you will observe for results.
26 Isolation of Fungi Air Expose the prepared plate of solid media in air for 5 min Close the lid and incubate at 28 C. After 2-5 days fungus observe for grow Soil Sprinkle a pinch of soil on the solid media plate Close the lid and incubate at 28 C After 2-3 days fungus observe for growth. fruit Clean the rotten part of fruit with alcohol Cut a piece and inoculate it on the media plate Then incubate,after 2-5 days fungus will grow
27 These plates of fungus will be incubated straight at 28 C for 24 hours and then stored at refrigerator until next week when you will observe for results.
28
29 Bacteria Fungi
30 أ.شروق الشهراني
320 MBIO Microbial Diagnosis. Aljawharah F. Alabbad Noorah A. Alkubaisi 2017
320 MBIO Microbial Diagnosis Aljawharah F. Alabbad Noorah A. Alkubaisi 2017 Primary Media for Isolation of Microorganisms As we know, many clinical specimens contain a mixed flora of microorganisms. Thus
More informationInoculate: Media. Physical State of Media: Liquid. The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory
The Five I s: Basic Techniques to Culture Microbes Tools of the Microbiology Laboratory 1. Inoculate 2. Incubate 3. Isolate 4. Inspect 5. Identify The Five I s: Inoculate Inoculate: Media Classified according
More informationMicrobiological Methods
Microbiological Methods Making Media Pouring Culture Plates Sterile Technique Inoculating Plates and Culture Tubes Use of a Plate Counter to Estimate Microbial Population Densities Sterile Technique Sterile
More informationBacterial Plate Preparation. ~ Using aseptic techniques ~
Bacterial Plate Preparation ~ Using aseptic techniques ~ Bacterial Plates Laboratory and research scientists have to prepare nutrient media to grow specific strains of bacteria for their research. To do
More informationMicrobiological Methods
Microbiological Methods Making Media Pouring Culture Plates Sterile Technique Inoculating Plates and Culture Tubes Use of a Plate Counter to Estimate Microbial Population Densities Culturing Microorganisms
More informationMICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA
MICROBIOLOGY #2 PREPERATION AND STERILIZATION OF CULTURE MEDIA When we receive a sample (ex. Urine sample) for detection, we cannot gram stain it right away if it requires to be inoculated because when
More informationPathogenic Bacteria. culture media. Components of the Typical Culture Medium: Culture Media Importance:
Level4 Lab2: Pathogenic Bacteria culture media Microorganisms, like all other living organisms, require basic nutrients for sustaining their life. All microorganisms have the same basic requirements but
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationLABORATORY #2 -- BIOL 111 BACTERIAL CULTIVATION & NORMAL FLORA
LABORATORY #2 -- BIOL 111 BACTERIAL CULTIVATION & NORMAL FLORA OBJECTIVES After completing this exercise you should be able to: 1. Identify various types of media 2. Isolate bacteria using aseptic technique.
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More informationCulture Media. Provide certain environmental conditions, nutrients & energy in order to grow and produce bacteria
Culture Media Culture Media Provide certain environmental conditions, nutrients & energy in order to grow and produce bacteria Different categories of media can be made according to the type and combination
More informationMICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods
Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most
More informationExercise 19. Fungi: Molds and Yeasts F10 Or The Rotten World Around Us
Exercise 19 119 Fungi: Molds and Yeasts F10 Or The Rotten World Around Us INTRODUCTION: Student Learning Objectives: After completing this exercise students will: a. Define the terms Saprophyte, Mycosis,
More informationCulturing microorganisms
Culturing microorganisms I. Historical development II. Problems and Solutions III. Studying microorganisms without a microscope -- culturing techniques A. How do you do it? B. Inoculation and isolation
More informationChapter 03 - Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of microorganisms
Microbiology: A Systems Approach 4th Edition Cowan Test Bank Completed download: https://testbankreal.com/download/microbiology-systems-approach-4thedition-test-bank-cowan/ (Downloadable package TEST BANK
More informationCulturing microorganisms
Culturing microorganisms I. Historical development II. Problems and Solutions III. Studying microorganisms without a microscope -- culturing techniques A. How do you do it? B. Inoculation and isolation
More informationDNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003
DNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003 Prepared by the Office of Biotechnology, Iowa State University TEACHER PREPARATION AND INSTRUCTION GUIDE Preparation for the DNA transformation
More informationGENUS STAPHYLOCOCCUS: Isolation and Identification
GENUS STAPHYLOCOCCUS: Isolation and Identification Staphylococcus is a genus of Gram +, nonspore-forming cocci belonging to the family Micrococcaceae that are often found as normal human microbiota of
More informationTRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE
TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE
More informationMicrobiology Chapter 2 Laboratory Equipment and Procedures 2:1 The Light Microscope MICROSCOPE: any tool with a lens to magnify and observe tiny
Microbiology Chapter 2 Laboratory Equipment and Procedures 2:1 The Light Microscope MICROSCOPE: any tool with a lens to magnify and observe tiny details of specimens Micro tiny, small Scope to see SIMPLE
More informationLab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl)
Lab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl) Some of the diseases that humans can contract from drinking polluted water include typhoid, dysentery,
More informationCulture Media A substance used to provide nutrients for the growth and multiplication of microorganisms. Types of Culture Media A) Based on their
CULTURE MEDIA Dr. C.SWAMINATHAN Assistant Professor PG and Research Department of Microbiology St. Joseph s College of Arts and Science (Autonomous) Cuddalore 607 001 Tamil Nadu, India Culture Media A
More informationCharacterization and Hemolytic Activity of Isolated Bacteria from Bats s Saliva Samples on the Menoreh Karst Area
ISBN 978-93-84422-41-7 Proceedings of 2015 International Conference on Biological and Environmental Science (BIOES 2015) Phuket, October 1-3, 2015, pp. 27-32 Characterization and Hemolytic Activity of
More informationMicroorganisms In Our Environment
PR015 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Microorganisms In Our Environment Teacher s Guidebook (Cat. # BE 106) think proteins!
More informationAdapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692
Biology 4B Laboratory Bacteriological Examination of Water Adapted from Biology 15 Laboratory Manual Supplement: Wrightsman, Ininns and Cannon-Moloznic, Saddleback College, CA 92692 Objectives Carry out
More informationASEPTIC TRANSFER & PURE CULTURE TECHNIQUES
ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES GENERAL GUIDELINES & REMINDERS: SAFETY: NO EATING OR DRINKING IN THE LAB! Wash your hands with soap both BEFORE and AFTER lab, and, in addition, when you have
More informationProject 7: Wound Cultures and Identification
Project 7: Wound Cultures and Identification Readings: https://labtestsonline.org/understanding/analytes/wound-culture/tab/test Identification of Gram-Positive & Gram-Negative Bacteria Guide to laboratory
More informationIsolation & Characterization of Bacteria
PR025 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Isolation & Characterization of Bacteria Teacher s Handbook (Cat. # BE 204) think proteins!
More informationnumber Done by Corrected by Doctor
L number Lab 2 Done by حسام أبو عوض Corrected by Mahdi sharawi Doctor In many cases we need to identify the type of bacteria causing an infection in order to be able to choose the right medication (antibiotic).
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationCULTURING MICROORGANISMS
CULTURING MICROORGANISMS Question practice Name: Class: Date: Time: 24 minutes Marks: 24 marks Comments: BIOLOGY ONLY Page of 2 The diagram shows a method used to grow pure cultures of a bacterium. (a)
More informationColiform bacteria are quantitated by the fractional gram pour plate technique (Note 1). Test tubes containing gas collector tubes (Durham Tubes)
Microbiological Methods IV-A- 1 (STANDARD PLATE COUNT METHOD) PRINCIPLE SCOPE Coliform bacteria are quantitated by the fractional gram pour plate technique (Note 1). The method is applicable to starches,
More informationMaking Saline SOLUTION. Lab Number 2 Part 1
Making Saline SOLUTION Lab Number 2 Part 1 Purpose The purpose of part 1 of this lab is to learn the proper way to make reagents that are needed for labs. Materials Need for the Lab are: Volumetric flasks
More informationECOS Inquiry Template
ECOS Inquiry Template 1. Contributor s Name: NATHAN GORDON 2. Name of Inquiry: NOT TOO HOT, NOT TOO COLD: THE EFFECTS OF TEMPERATURE ON SOIL BACTERIA 3. Goals and Objectives: a. Inquiry Questions: 1. How
More informationSELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7
SELECTED QUESTIONS F ROM OLD MICRO 102 QUIZZES PART I EXPERIMENTS 1 THROUGH 7 Question numbers refer to the applicable experiment. Questions with blanks are multiple true-false questions unless otherwise
More informationSCHEDULE. Friday: Pet Investigations: Plate counts - how to know how many clones of your pet you have (pg. 9-10)
SCHEDULE Wednesday: Pet Investigations: Phenol Red Broth with Durham tubes (pg. 3-4) Oxidation/Fermentation Agar (pg. 5-6) Anaerobic Growth (pg. 7) Growth in Liquid Culture (pg. 8-9) Friday: Pet Investigations:
More informationNATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA. National Food Safety Standard
GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 食品安全国家标准食品微生物学检验大肠埃希氏菌计数
More informationPet Microbe 1: Lab Guide
Experiment 2: Pet microbes: A lesson in taxonomy This experiment will start with three lab periods and then continue throughout the semester. What's in a name? Names help us communicate a lot of information
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationCh 6. Microbial Growth
Ch 6 Microbial Growth Student Learning Outcomes: Classify microbes into five groups on the basis of preferred temperature range. Explain the importance of osmotic pressure to microbial growth. Provide
More informationPRESERVATIVE EFFICACY TEST FOR COSMETIC PRODUCT
1 SCOPE AND FIELD OF APPLICATION To determine the efficacy of the antimicrobial activity of preservatives used in cosmetic products. The method covers the determination of the suitability of preservation
More informationFoundations in Microbiology Seventh Edition
Lecture PowerPoint to accompany Foundations in Microbiology Seventh Edition Talaro Chapter 3 Tools of the Laboratory: The Methods for Studying Microorganisms Copyright The McGraw-Hill Companies, Inc. Permission
More informationHeat Shock Proteins in Yeast (2012)
MASSACHUSETTS INSTITUTE OF Technology Department of Biology Heat Shock Proteins in Yeast (2012) Summary Lydia Breen (Stoneham High School) Mary Brunson (Brookline High School) Yeast is a single-celled
More informationBiotechnology In Your Mouth
PR005 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biotechnology In Your Mouth Teacher s Guidebook (Cat. # BE 102) think proteins! think
More informationLab Exercise #4 Microbial Control Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic
Lab Exercise #4 Control of Microorganisms: Physical, Chemical and Chemotherapeutic I. OBJECTIVES: Investigate the effectiveness various agents of control. Assess the effectiveness of heat in killing vegetative
More informationBacterial Transformation Protocol 2
26 BACTERIAL TRANSFORMATION USING FLUORESCENT PROTEIN Bacterial Transformation Protocol 2 Group # Role in Group Materials Reader Timer Technician Student Name Materials checklist (1) ScienceBridge Transformation
More informationAbout Science Prof Online PowerPoint Resources
About Science Prof Online PowerPoint Resources Science Prof Online (SPO) is a free science education website that provides fully-developed Virtual Science Classrooms, science-related PowerPoints, articles
More informationGUIDELINES FOR WRITING A LAB REPORT FOR BIOL 215L (MICROBIOLOGY FOR HEALTHCARE PROFESSIONALS)
GUIDELINES FOR WRITING A LAB REPORT FOR BIOL 215L (MICROBIOLOGY FOR HEALTHCARE PROFESSIONALS) Your lab report will focus only on your unknown bacteria, which you collected, cultured, isolated, analyzed,
More information90927 Demonstrate understanding of biological ideas relating to micro-organisms COLLATED QUESTIONS
90927 Demonstrate understanding of biological ideas relating to micro-organisms COLLATED QUESTIONS DIGESTION AND REPRODUCTION PROCESSES (2013:1) (a) Describe the processes of digestion and reproduction
More informationLab Three :. Sensitivity test:
Lab Three :. Sensitivity test: Or Diffusion Test: Antibiotic sensitivity test: is a laboratory method for determining the susceptibility of organisms to therapy with antibiotics, Antibiotic susceptibility
More informationLABORATORY EXERCISES. to accompany MICROBIOLOGY LABORATORY
LABORATORY EXERCISES to accompany MICROBIOLOGY LABORATORY BSL 214 Professor Susan C. Kavanaugh Bluegrass Community & Technical College Fall 2008 INTRODUCTION TO CULTURING, MEDIA, AND ASEPTIC TECHNIQUES
More informationLABORATORY EXERCISES. to accompany MICROBIOLOGY LABORATORY
LABORATORY EXERCISES to accompany MICROBIOLOGY LABORATORY BSL 214 Professor Susan C. Kavanaugh Bluegrass Community & Technical College 2010 INTRODUCTION TO CULTURING, MEDIA, AND ASEPTIC TECHNIQUES Even
More informationMicrobial assay measures the activity of antibiotics (Extent of ability to inhibit
6. MICROBIOLOGICAL EVALUATION Microbial assay measures the activity of antibiotics (Extent of ability to inhibit the growth of micro organism) or vitamins and amino acids (Extent to support the growth
More informationDocument No. FTTS-FA-001. Specified Requirements of Antibacterial Textiles for General Use
1. Purpose and Scope This criterion is applicable to the evaluation and testing of antibacterial activity of textile for general use. The quantitative evaluation of antibacterial activity is judged by
More informationM I C R O B I O L O G Y
ninth edition TORTORA FUNKE CASE M I C R O B I O L O G Y a n i n t r o d u c t i o n 6 Microbial Growth PowerPoint Lecture Slide Presentation prepared by Christine L. Case Microbial Growth Microbial growth
More informationDiagnostic Microbiology
Diagnostic Microbiology Identification of Microbes Lecture: 2 Phenotypic characteristics: A- Morphology Macroscopic (Cultural Characteristics) Culture Media: Providing Nutrients requirements to microbes
More informationMICROBIAL GROWTH. Dr. Hala Al-Daghistani
MICROBIAL GROWTH Dr. Hala Al-Daghistani Microbial Growth Microbial growth: Increase in cell number, not cell size! Physical Requirements for Growth: Temperature Minimum growth temperature Optimum growth
More informationLAB NOTES FOR EXAM 1 SECTION
LAB NOTES FOR EXAM 1 SECTION EX. 2-1: DIVERSITY AND UBIQUITY OF MICROOGANISMS Purpose: Microorganisms are found everywhere in the environment around us. To demonstrate this and to get a taste of the different
More informationMicrobial Nutrition and Growth
PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 6 Microbial Nutrition and Growth CSLO 5. Give examples of the range of metabolic diversity
More informationDiagnostic Microbiology
Diagnostic Microbiology Identification of Microbes Lecture: 1 Out lines What is expected out of this course??? At the end of this course, you will be able to apply Conventional/ Molecular diagnostic methods
More informationMethods of Culturing Microorganisms. Chapter 3. Five Basic Techniques of Culturing Bacteria. Topics
Chapter 3 Topics Methods of Culturing Microorganisms Microscope (History, Types, Definitions) Staining (Gram s) Methods of Culturing Microorganisms Five basic techniques of culturing Media Microbial growth
More information<51> Uji Batas Mikroba Farmakope Indonesia ed.4 (<61>Microbial Limit Test) and Identification method. Marlia Singgih Wibowo School of Pharmacy ITB
Uji Batas Mikroba Farmakope Indonesia ed.4 (Microbial Limit Test) and Identification method Marlia Singgih Wibowo School of Pharmacy ITB Why we need Microbial Limit Test? To predict number of
More informationSome Industrially Important Microbes and Their Products
2 Some Industrially Important Microbes and Their Products 2.1. ENZYME PRODUCING MICROBES Type of enzyme Substrate Microorganism Amylase Starch Saccharomyces diastaticus Protease Proteins Bacillus sp. Lipase
More informationBiology 322 Fall 2010 Transfer of genetic information in the bacterium Escherichia coli: Part I
Biology 322 Fall 2010 Transfer of genetic information in the bacterium Escherichia coli: Part I REQUIRED Reading Assignments: Superbugs on the Hoof http://fire.biol.wwu.edu/trent/trent/superbugs.pdf Triple
More informationMicrobial Nutrition and Growth
PowerPoint Lecture Presentations prepared by Mindy Miller-Kittrell, North Carolina State University C H A P T E R 6 Microbial Nutrition and Growth CHNO Growth Requirements Nutrients: Chemical and Energy
More informationAseptic Techniques. A. Objectives. B. Before coming to lab
Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note
More informationBiology 123 Microbiology Spring 2015
Parkland College Biology Courses Natural Sciences Courses 2015 Biology 123 Microbiology Spring 2015 Vikki Crnekovic Parkland College, vcrnekovic@parkland.edu Tiffany Gibson Parkland College, TGibson@parkland.edu
More informationCOUNT METHOD 5.0 OBJECTIVES 5.1 INTRODUCTION 5.2 PRINCIPLE. Structure
Food Microbiology EXPERIMENT 5 STANDARD PLATE COUNT METHOD Structure 5.0 Objectives 5.1 Introduction 5.2 Principle 5.3 Materials Required 5.4 Procedure 5.4.1 E-coli Culture 5.4.2 Food Samples 5.5 Observations
More informationLaboratory Procedure October 1999 HEALTH PROTECTION BRANCH OTTAWA ANALYSIS OF SPROUTS FOR COLIFORMS, ESCHERICHIA COLI, AND KLEBSIELLA PNEUMONIAE..
Government of Canada Gouvernement du Canada Laboratory Procedure MFLP-64 October 1999 HEALTH PROTECTION BRANCH OTTAWA ANALYSIS OF SPROUTS FOR COLIFORMS, ESCHERICHIA COLI, AND KLEBSIELLA PNEUMONIAE.. Don
More informationCulturing microorganisms may be hazardous
Practical 8 - S(d) The Effect of Penicillin on Bacterial Growth In this practical focuses on the practical skills of: Planning defining the problem You will be developing other assessed skills throughout
More informationIdentification of Indigenous Unknowns
Unknown Lab Report III Identification of Indigenous Unknowns Introduction: In a scientific field with so much genetic variation and rapidly evolving species, it has become increasingly difficult to correctly
More informationBiology 123 Microbiology Fall 2015
Parkland College Biology Courses Natural Sciences Courses 2015 Biology 123 Microbiology Fall 2015 Vikki Crnekovic Parkland College, vcrnekovic@parkland.edu Recommended Citation Crnekovic, Vikki, "Biology
More informationMOLEBIO LAB #12: Bacterial Culture Techniques Part I
MOLEBIO LAB #12: Bacterial Culture Techniques Part I Introduction: This lab introduces an introduction to plating and culturing E. coli on LB agar plates such that single cells can be isolated from one
More informationTest Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002
Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 FTTS-FA-002 Antibacterial Textiles for Medical Use Antibacterial Textiles suppress and even kill harmful bacteria
More informationProject 5: Urine Cultures and Identification
Project 5: Urine Cultures and Identification Readings: http://www.webmd.com/a-to-z-guides/urine-culture http://www.medscape.com/viewarticle/558845 (Listen to the two lectures by Dr. Robert A. Weinstein.)
More informationENVR 421 Laboratory #1: Basic Bacteriology Techniques
ENVR 421 Laboratory #1: Basic Bacteriology Techniques Introduction The purpose of this laboratory exercise is to familiarize you with two fundamental bacteriology techniques: the streak plate and the spread
More informationSample collection & laboratory methods for identification bacteria. Lab. 2 A.T. Samira
Sample collection & laboratory methods for identification bacteria Lab. 2 A.T. Samira Learning objectives At the end of the presentation, participants should understand the: Procedures, preparation, processing
More informationMicrobiology sheet (6)
Microbiology sheet (6) Made by marah marahleh corrected by : abd. Salman DATE :9/10/2016 Microbial growth / control of microbial growth 1 The method of counting bacteria is divided into: 1) direct 2) indirect
More informationBacterial Transformation and Protein Purification
Bacterial Transformation and Protein Purification Group 4 Natalie Beale Gregory A. Pate Justin Rousseau Dohee Won Introduction The purpose of this experiment is to perform a genetic transformation and
More informationBD Mac Conkey II Agar / Columbia CNA Agar with 5% Sheep Blood (Biplate)
PA-254447.02-1 - INSTRUCTIONS FOR USE READY-TO-USE PLATED MEDIA PA-254447.02 Rev.: Sep 2011 BD Mac Conkey II Agar / Columbia CNA Agar with 5% Sheep Blood (Biplate) INTENDED USE BD Mac Conkey II Agar /
More informationLab Date Experiment Reports, Midterms, Reminders
Lab Date Experiment Reports, Midterms, Reminders Lab 1 Lab 2 Jan 5-6 Jan 7-8 o Registration, introductory remarks, safety lecture, etc. Sterile technique. o Expt 1: Microbes in the environment o Expt 2:
More informationGB Translated English of Chinese Standard: GB
Translated English of Chinese Standard: GB4789.35-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.35-2016 National food safety standard
More informationActivity Sheet 2 BACTERIA TAKE-DOWN Problem: What metals are the most effective in inhibiting bacterial growth?
Activity Sheet 2 BACTERIA TAKE-DOWN Problem: What metals are the most effective in inhibiting bacterial growth? Background: Germs are EVERYWHERE!!! We have hear this all before. Quite often illnesses and
More informationLevel 1 Biology, 2012
90927 909270 1SUPERVISOR S Level 1 Biology, 2012 90927 Demonstrate understanding of biological ideas relating to micro-organisms 9.30 am Thursday 15 November 2012 Credits: Four Achievement Achievement
More information1 Demonstrate knowledge and understanding related to microbiological techniques. 2 Carry out techniques related to microbiology.
National Unit Specification: general information NUMBER D039 11 COURSE Biotechnology (Intermediate 2) SUMMARY This unit seeks to develop knowledge, understanding and practical skills in the microbiological
More informationCOVENANT UNIVERSITY OMEGA SEMESTER TUTORIAL KIT (VOL. 2) 100 LEVEL
COVENANT UNIVERSITY OMEGA SEMESTER TUTORIAL KIT (VOL. 2) P R O G R A M M E : M I C R O B I O LO GY 100 LEVEL DISCLAIMER The contents of this document are intended for practice and learning purposes at
More informationM. Dalbey/Bio 105M Isolation of E. coli - Isolation of E. coli from an Environmental Sample
Isolation of E. coli from an Environmental Sample We want to expand our horizons a bit beyond the domesticated lab strains of E. coli. In this exercise you will isolate "wild" E. coli strains from an environmental
More informationSYNTHETIC BIOLOGY AND THE HIGH SCHOOL CURRICULUM: LAB 1 _Lab_1
SYNTHETIC BIOLOGY AND THE HIGH SCHOOL CURRICULUM: LAB 1 http://openwetware.org/wiki/synthetic_biology_and_the_high_school_curriculum: _Lab_1 LAB 1: Eau that smell Comparing 2 competing designs to optimize
More informationIdentifying Enterobacter aerogenes from a Mixed Culture of Unknown Gram Positive and Gram Negative Bacteria Kevin Le November 13, 2013
Identifying Enterobacter aerogenes from a Mixed Culture of Unknown Gram Positive and Gram Negative Bacteria Kevin Le November 13, 2013 PURPOSE The focus of this study is to be able to identify an unknown
More informationExercise 13 DETERMINATION OF MICROBIAL NUMBERS
Exercise 13 DETERMINATION OF MICROBIAL NUMBERS Introduction When biologists discuss the growth of microorganisms (microbial growth), they are actually referring to population size rather than to the size
More informationMedical Mycology. Lab (1)
Medical Mycology Lab (1) 1-Introduction Mycology - the study of fungi Fungi - molds and yeasts Molds - exhibit filamentous type of growth Yeasts - pasty or mucoid form of fungal growth 50,000 + valid species;
More informationTransforming E. Coli with pglo Plasmids
Name: Transforming E. Coli with pglo Plasmids AP Biology Transformation Background: Transformation is a process of transferring genetic information from one organism to another. In bacteria, a small circular
More informationSerial dilution and colony count (Viable count) Pour plate. Spread plate Membrane filtration. Turbidity. Microscopic cell count
Aljawharah Alabbad 2016 Serial dilution and colony count (Viable count) Pour plate Spread plate Membrane filtration Turbidity Microscopic cell count Many studies require the quantitative determination
More informationMACROPHAGE KILLING ASSAY
MACROPHAGE KILLING ASSAY Updated by: Joseph Chon Date: July 2018 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND This protocol is used to determine a macrophage population
More informationMiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3. Introduction
MiSP Evolution by Natural Selection / Bacterial Resistance Teacher Guide, L1 L3 Introduction This unit uses the development of resistance to antimicrobials as an example of natural selection. The students
More informationBacteria Introduction Bacteria are unicellular micro-organisms ranging in length from a few micrometers to half a millimeter. They come in a variety
Kingdom Bacteria Bacteria Introduction Bacteria are unicellular micro-organisms ranging in length from a few micrometers to half a millimeter. They come in a variety of different shapes (cocci, bacilli
More informationcommercial and biological interest. The water usually contains which have been tested tolerate no more than 6 per cent salt and
THE BACTERIOSTATIC AND BACTERICIDAL ACTION OF GREAT SALT LAKE WATER CLAUDE E. ZOBELL AND D. QUENTIN ANDERSON Scripps Institution of Oceanography, University of California AND W. WHITNEY SMITH Bacteriology
More informationINHIBITION OF A STAPHYLOCOCCAL HEMOLYSIN BY A SOLUBLE SUBSTANCE PRODUCED BY A NONHEMOLYTIC MICROCOCCUS SPECIES
INHIBITION OF A STAPHYLOCOCCAL HEMOLYSIN BY A SOLUBLE SUBSTANCE PRODUCED BY A NONHEMOLYTIC MICROCOCCUS SPECIES PINGHUI LIU' Kitasato Institute for Infectious Diseases, Tokyo, Japan Received for publication
More informationSection 8: Refined sugar p 1/5
Section 8: Refined sugar p 1/5 1. Rationale Method 8.19 Refined sugar: total thermophilic organisms, flat sour spores, anaerobic organisms producing sulphide and anaerobic organisms producing gas The method
More information