Plasmid Midiprep Purification Kit
|
|
- Horace Rice
- 5 years ago
- Views:
Transcription
1 Plasmid Midiprep Purification Kit Cat. # : DP01MD/ DP01MD-100 Size : 20/100 Reactions Store at RT For research use only 1
2 Description: The Plasmid Midiprep Purification Kit provides simple rapid protocol and materials to purify DNA from 20~100 ml bacteria liquid culture. The yield of DNA, from 30 μg~300 μg, is quantitative and reproducible. The obtained DNA can be used for many applications, including PCR, restriction enzyme digestion, cloning, automated DNA sequencing, southern blot, in vitro transcription assay, and transfection. Components of the Kit: DP01MD DP01MD Solution I 110 ml 550 ml 2. Solution II 110 ml 550 ml 3. Solution III 110 ml 550 ml 4. Binding Solution 33 ml 165 ml 24 ml 120 ml 5. Endotoxin Removal (add 16 ml isopropanol (add 80 ml isopropanol Wash Solution before use) before use) 6. Wash Solution 10 ml (add 40 ml Ethanol before use) 50 ml (add 200 ml Ethanol before use) 7. Elution Solution 20 ml 100 ml 8. Midi-Spin Column 20 pcs 100 pcs 9. Collection Tube 20 pcs 100 pcs 10. RNase A powder 40 mg / btl. 180 mg x mg x 1 * Store RNase A Powder at -20 C and store all other kit components at room temperature (25~28 C). * If precipitates have formed in solution II, warm the buffer at 35 C in a water bath until fully dissolved. 2
3 Before staring this procedure: Dissolve RNaseA Powder in Solution I: For DP01MD, add 1 ml Solution I to each tube of RNase A Powder, vortex until completely dissolved. Transfer all the solution to Solution I and store at 4 C. For DP01MD-100, add one tube of 180 mg RNase A Powder to the 500 ml Solution I bottle, and add one tube of 18 mg RNase A Powder into the 50 ml Solution I bottle. General Procedure: * Use 20~50 ml of bacteria culture with high copy plasmid, and 50~100 ml of bacteria culture with low copy plasmid. Excess volume will result in incomplete cell lysis, reducing the yield and quality of plasmid. * Do not use sample volume more than recommended to prevent clogging of spin- column. 1. Pellet down cells from 20~100 ml liquid culture by centrifugation at 12,000~14,000 x g for 5 min. Pour off the supernatant. 2. Completely resuspend the cell pellet in 5 ml of Solution I by pipetting or vortexing. Incubate at RT for 10 min for uniform and complete suspension of cell especially for high density (> 3 x 10 9 cells/ml) or large volume (> 50 ml) cultures. 3. Add 5 ml of Solution II and mix by inverting the tube 6~8 times (the cell suspension should turn clear immediately). Incubate at RT for 2~4 min. * Incubation >5 min may promote partial denaturation of DNA and hence may affect manipulation like restriction digestion. 4. Add 5 ml of Solution III and mix it by inverting the tube 6~8 times. 5. Centrifuge the lysate at 12,000~14,000x g for 10 min at 4 C. A compact white pellet will form along the side or at the bottom of the tube. 6. Carefully transfer the supernatant to a new 50 ml centrifuge tube and 3
4 add 10 ml of isopropanol into the tube. Mix the solution by inverting the tube several times. A few of the floating white pellets may be transferred to the tube. This does not affect the purification of plasmid. 7. Centrifuge the lysate at 12,000~14,000x g for 10 minutes at 4 C. A white DNA pellet will form at the bottom of the tube. Discard the supernatant. 8. Add 500 μl of Binding Solution to resuspend the DNA pellet completely by pipetting gently. * Most DNA pellets will be at the bottom. For DNA pellets that are stuck to the wall of the tube, wash down and dissolve. 9. Insert the Midi-Spin Column into a Collection Tube and transfer the resuspended DNA solution to the Midi-Spin Column. Wait for 1 min for equilibration with the membrane and spin at top speed (12,000~14,000x g) for 2 min. 10. Discard the filtrate and add 500 μl of Binding Solution. Wait for 2 min for equilibration with the membrane and spin at top speed for 2 min. If endonuclease free is desired, add 500 μl binding solution to wash again. * This step eliminates the endonuclease contamination from EndA + strain (e.g. BL21 (DE3), HB101 and JM series (e.g. JM109). 11. Discard the filtrate and add 650 μl of Endotoxin Removal Wash Solution (with isopropanol added). Wait for 2 min for equilibration with the membrane and centrifuge at top speed for 2 min. Discard the filtrate. Repeat this step once more. 12. Discard the filtrate and add 600 μl of Wash Solution to the spin column. Centrifuge for 2 min and discard the filtrate. Repeat this step once more. * For transfection purpose, wash two more times, i.e. three washes in total. 13. Discard the filtrate and centrifuge for additional 5 min at top speed to remove residual ethanol. 14. Discard the collection tube and incubate the Midi-Spin Column at 60~65 C for 10 min to evaporate any residual ethanol. Do not incubate 4
5 the column > 15 min to prevent the DNA pellet from becoming too hard to elute. * Make sure to remove all ethanol completely, which may be important for automatic sequencing and transfection. 15. Transfer the Midi-Spin Column into a new 1.5 ml microcentrifuge tube and add 150~300 μl preheated (60~65 C) Elution Solution or TE or H 2 O (ph 7.0~8.5) into the column. Wait for 3 min and centrifuge at top speed for 2 min to elute DNA. Store the elute plasmid DNA at -20 C. *Notes: To increase the DNA concentration, reload the eluted DNA Solution into the Midi-Spin Column, and incubate at 60~65 C for 3 min and then spin again to elute DNA. 5
6 Troubleshooting Guide Low or no yield Comments and Suggestion a) Plasmid did not propagate Inoculate the liquid medium containing recommended antibiotic with single colony of bacterial cells from a freshly streaked plate and grow the culture under appropriate conditions. b) Overgrown bacteria Overgrowth of culture leads to lysis of cells and degradation of DNA. Do not grow cultures longer than 12~16 hours. c) Not enough bacterial cells Ensure optimum growth of culture (OD 600 > 1) by incubating the culture overnight at recommended temperature with vigorous shaking. d) Column clogged due to excess sample volume Do not use sample volume more than recommended to prevent clogging of spin- column. e) Low-copy-number plasmid When using low-copy-number plasmids, use larger culture volumes. Even with larger culture volumes, yields of low-copy-number plasmids will be lower than those of high-copy-number plasmids. f) Poor cell lysis 1) Excessive growth of bacteria. The culture 6
7 medium should contain recommended antibiotic. 2) Poor cell suspension. Ensure complete resuspension of bacterial pellet by votexing or pipetting before adding Solution II. g) Incomplete DNA Elution 1) If plasmid is larger than 7 Kb, use preheated Elution solution (60~65 C ) to elute. 2) DNA should be eluted only with a low-salt buffer [e.g., Elution solution (10 mm Tris Cl, ph 8.5) or water]. Elution efficiency is dependent on ph. The maximum efficiency is achieved between ph 7.0 and 8.5. When using water for elution, make sure that the ph value is within this range. 3) Ensure that Elution solution is added onto the center of the membrane and is completely absorbed. Genomic DNA contamination a) Overgrown bacteria Overgrowth of culture leads to lysis of cells and degradation of DNA. Do not grow cultures longer than 12~16 hours. b) Lysate prepared incorrectly 1) The lysate must be handled gently after addition of Solution II to prevent genomic 7
8 DNA shearing. 2) Lysis in step 3 must not exceed 5 minutes. c) Solution III added incorrectly Upon addition of Solution III, mix immediately, but gently. RNA contamination RNase A digestion insufficient 1) Ensure that RNase A is added to Solution I before use and stored at 4 C. 2) If Solution I containing RNase A is stored beyond a year, add additional RNase A to Solution I to make 360 μg/ml final concentration of RNase A. 3) Reduce the culture volume, if necessary. 8
9 9
Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only
Plasmid Midiprep Plus Purification Kit Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Plus Purification Kit provides simple
More informationPlasmid Maxiprep Plus Purification Kit
Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,
More informationPlasmid Maxiprep Plus Purification Kit. Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only
Plasmid Maxiprep Plus Purification Kit Cat. # : DP01MX-P10/ DP01MX-P20 Size : 10/20 Reactions Store at RT For research use only 1 Description: The Plasmid Maxiprep Plus Purification Kit provides simple,
More informationBacteria Genomic DNA Purification Kit
Bacteria Genomic DNA Purification Kit Cat #:DP025/ DP025-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Bacteria Genomic DNA Purification Kit provides a rapid, simple, and
More informationPlasmid DNA Extraction Midiprep Kit
BIO BASIC Plasmid DNA Extraction Midiprep Kit 9K-006-0027s (1 prep) 9K-006-0027 (25 preps) Toll Free 1-877-956-7778 ForOrder Research Use Only www.feldan.com 1 Index Plasmid DNA Extraction Midiprep Kit
More informationPlasmid DNA Extraction Miniprep Kit
BIO BASIC Plasmid DNA Extraction Miniprep Kit 9K-006-0009s (10 preps) 9K-006-0009 (100 preps) 9K-006-0010 (200 preps) For Research Use Only Index Plasmid DNA Extraction Miniprep Kit Introduction 3 Important
More informationFavorPrep Endotoxin-Free Plasmid DNA Extraction Maxi Kit. User Manual
TM FavorPrep Endotoxin-Free Plasmid DNA Extraction Maxi Kit User Manual Cat. No.: FAPDE 003-EF (10 Preps) For Research Use Only v.1005-1 Introduction TM The FavorPrep Endotoxin-Free Plasmid DNA Extraction
More informationTissue & Cell Genomic DNA Purification Kit. Cat. #:DP021/ DP Size:50/150 reactions Store at RT For research use only
Tissue & Cell Genomic DNA Purification Kit Cat. #:DP021/ DP021-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Tissue & Cell Genomic DNA Purification Kit provides a rapid,
More informationEndoFree Maxi Plasmid Kit
EndoFree Maxi Plasmid Kit For purification of ultrapure plasmid DNA with high yields www.tiangen.com/en DP121221 EndoFree Maxi Plasmid Kit Kit Contents Storage (Spin Column) Cat.no. DP117 Contents RNase
More informationPlus Blood Genomic DNA Purification Kit
Plus Blood Genomic DNA Purification Kit Cat #:DP023P/ DP023P-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Plus Blood Genomic DNA Purification Kit provides a rapid, simple
More informationPresto Mini Plasmid Kit
Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured
More informationI-Blue Midi Plasmid Kit. I-Blue Midi Plasmid Kit. (Endotoxin Free) IBI SCIENTIFIC. Instruction Manual Ver For Research Use Only.
Instruction Manual Ver. 05.11.17 For Research Use Only I-Blue Midi Plasmid Kit & I-Blue Midi Plasmid Kit (Endotoxin Free) IB47180, IB47190 (2 Preparation Sample Kit) IB47181, IB47191 (25 Preparation Kit)
More informationGeneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free)
Geneaid Maxi Plasmid Kit & Geneaid Maxi Plasmid Kit (Endotoxin Free) PM002, PME02 (2 Preparation Sample Kit) PM010, PME10 (10 Preparation Kit) PM025, PME25 (25 Preparation Kit) Instruction Manual Ver.
More informationQIAfilter Plasmid Midi Kit (Cat #: 12243)
QIAfilter Plasmid Midi Kit (Cat #: 12243) Things to do before starting Add the provided RNase A solution to Buffer P1 before use. Use one vial of RNase A (centrifuge briefly before use) per bottle of Buffer
More informationTIANpure Mini Plasmid Kit
TIANpure Mini Plasmid Kit For purification of molecular biology grade DNA www.tiangen.com PP080822 TIANpure Mini Plasmid Kit Kit Contents Storage Contents RNaseA (10 mg/ml) Buffer BL Buffer P1 Buffer P2
More informationHurricane Miniprep Kit PROTOCOL
Hurricane Miniprep Kit PROTOCOL Description: The Hurricane Miniprep Kit is designed for purification of up to 25 ug of high purity plasmid DNA from a starting volume of 2-5 ml of bacterial culture. The
More informationTIANpure Midi Plasmid Kit
TIANpure Midi Plasmid Kit For purification of molecular biology grade DNA www.tiangen.com Kit Contents Storage TIANpure Midi Plasmid Kit Contents RNaseA (10 mg/ml) Buffer BL Buffer P1 Buffer P2 Buffer
More informationKits for isolation of plasmid DNA in medium scale
PLASMID MIDI KIT PLASMID MIDI ENDOTOXIN-FREE Cat. No. EM16 / EM17 Version: 1.2014 Kits for isolation of plasmid DNA in medium scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MIDI KIT PLASMID
More informationMicro-Elute DNA Clean/Extraction Kit
Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/Gel Extraction/DNA Clean-Up /Concentration Kit) Cat. # : DP034ME/ DP034ME-300 Size : 50/300 Reactions Store at RT For research use only 1 Description:
More informationEasy Tissue & Cell Genomic DNA Purification Kit. Cat. #:DP021E/ DP021E-150 Size:50/150 reactions Store at RT For research use only
Easy Tissue & Cell Genomic DNA Purification Kit Cat. #:DP021E/ DP021E-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Easy Tissue & Cell Genomic DNA Purification Kit is ideal
More informationTIANpure Mini Plasmid Kit II
TIANpure Mini Plasmid Kit II For purification of molecular biology grade DNA www.tiangen.com/en DP140918 Kit Contents Storage TIANpure Mini Plasmid Kit II Contents RNase A (10 mg/ml) Buffer BL Buffer P1
More informationMag-Bind Ultra-Pure Plasmid DNA 96 Kit. M x 96 preps M x 96 preps
M1258-00 1 x 96 preps M1258-01 4 x 96 preps December 2015 Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Plasmid Protocol with Lysate Clearance via Centrifugation...5
More informationFast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using
More informationE.Z.N.A. Endo-free Plasmid DNA Mini Kit I
E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps November
More informationTaKaRa MiniBEST Plasmid Purification Kit Ver.4.0
Cat. # 9760 For Research Use TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0 Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 4 IV. Preparation
More informationAurum Plasmid Mini Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510)
Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 Aurum Plasmid Mini Kit Instruction Manual For technical service, call your local Bio-Rad office, or
More informationE.Z.N.A. Endo-free Plasmid DNA Mini Kit I
E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps May 2013
More informationBio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510) Rev C
Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 4110111 Rev C Aurum Plasmid Mini Kit Instruction Manual For technical service, call your local Bio-Rad
More informationEasyPrep TM Plasmid Maxiprep Manual
EasyPrep TM Plasmid Maxiprep Manual Catalog#: PD05-01, PD05-02 PD05-11, PD05-12 Bioland Scientific LLC 14925 Paramount Blvd., Suite C Paramount, CA 90723 USA Tel: (877) 603-8882 Fax: (562) 733-6008 Email:
More informationThe fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA.
INSTRUCTION MANUAL Zyppy Plasmid Midiprep Kit Catalog Nos. D4025 & D4026 (Patent Pending) Highlights The fastest, simplest method for purifying the highest quality endonuclease-free plasmid DNA. Pellet-Free
More informationAuPreP Plasmid Midi Kit Cat. #: PMD12-143LT (25 preps/kit) PMD12-145LT (50 preps/kit)
AuPreP Plasmid Midi Kit Cat. #: PMD12-143LT (25 preps/kit) PMD12-145LT (50 preps/kit) AuPreP Plasmid Midi Kit is designed for rapid isolation of plasmid DNA of superior quality from an average of 25-100
More information7.13 Experimental Microbial Genetics
MIT OpenCourseWare http://ocw.mit.edu 7.13 Experimental Microbial Genetics Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms. 7.13 Fall 2008 Page
More informationPlus DNA Clean/Extraction Kit
Plus DNA Clean/Extraction Kit Cat. # : DP034P/ DP034P-300 Size : 50/300 Reactions Store at RT For research use only 1 Description: The Plus DNA Clean/Extraction Kit is designed to extract DNA fragments
More informationGET Plasmid DNA 96 Well
187PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name GET Plasmid DNA 96 Well For High Yield & Quality Plasmid DNA Extraction (Cat. # 786
More informationPlasmid Miniprep Kit
Plasmid Miniprep Kit Catalog No. Specification Storage/Shelf life EP001-50T 50T Room temperature/1 year EP001-200T 200T Room temperature/1 year Introduction In this kit, the plasmid DNA was purified rapidly
More informationKits for isolation of plasmid DNA in large scale
PLASMID MAXI KIT PLASMID MAXI ENDOTOXIN-FREE Cat. No. EM18 / EM19 Version: 1.2014 Kits for isolation of plasmid DNA in large scale EXTRACTME is a registered trademark of BLIRT S.A. PLASMID MAXI KIT PLASMID
More informationEasyPrep TM EndoFree Plasmid Maxiprep Purification Protocol
Table of Contents EasyPrep TM EndoFree Plasmid Maxiprep Purification Protocol Introduction... 3 Important Notes.... 3 Before Starting... 4 PD05-21, PD05-22 PD05-31, PD05-32 Kit Contents... 5 EndoFree Plasmid
More informationE.Z.N.A. HP Plasmid DNA Mini Kit I
E.Z.N.A. HP Plasmid DNA Mini Kit I D7043-00 5 preps V - Spin D7043-01 50 preps V - Spin E.Z.N.A. HP Plasmid DNA Mini Kit II D7045-00 5 preps V - Spin D7045-02 200 preps V - Spin July 2013 E.Z.N.A. HP
More informationThe fastest, easiest, most reliable method for purification of up to 1.2 mg of ultra-pure endotoxin-free plasmid DNA.
INSTRUCTION MANUAL ZymoPURE - Express Plasmid Midiprep Kit Catalog No. D4213 (Patent Pending) Highlights Innovative ZymoPURE - Express pellet-free procedure bypasses the standard cell-pelleting and resuspension
More informationIntroduction. 2. Important Notes.. 2. Storage and Stability Before Starting Kit Contents Safety Information.. 5
Contents Introduction. 2 Important Notes.. 2 Storage and Stability... 3 Before Starting... 4 Kit Contents... 5 Safety Information.. 5 EZgene EndoFree Plasmid Midiprep Protocol... 6 A: Removal of Endotoxin
More informationEZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications
More informationEZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 5.0 Rev 03/25/205 Table of Contents Introduction 2 Limitations of Use 2 Features 2 Applications 2 Storage 2
More informationFast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.
INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge
More informationFast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge spin-column.
INSTRUCTION MANUAL ZymoPURE II Plasmid Maxiprep Kit Catalog Nos. D4202 & D4203 (Patent Pending) Highlights Fast, easy, reliable, ultra-pure transfection grade plasmid DNA Maxiprep using a microcentrifuge
More informationE.Z.N.A. Bacterial DNA Kit. D preps D preps D preps
E.Z.N.A. Bacterial DNA Kit D3350-00 5 preps D3350-01 50 preps D3350-02 200 preps April 2017 E.Z.N.A. Bacterial DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability....3
More informationE.Z.N.A. Bacterial DNA Kit. D preps D preps D preps
E.Z.N.A. Bacterial DNA Kit D3350-00 5 preps D3350-01 50 preps D3350-02 200 preps April 2017 E.Z.N.A. Bacterial DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability....3
More informationSpecifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18050 Product Insert The BAC DNA
More information96 well Plant Genomic DNA Purification Kit
96 well Plant Genomic DNA Purification Kit Cat. #: DP022-9602/ DP022-9608 Size: 2 x 96 / 8 x 96 Reactions Store at RT For Research Use Only 2015.10.08 1 Description : The 96 well Plant Genomic DNA Purification
More informationE.Z.N.A. Yeast RNA Kit. R preps R preps
R6870-00 5 preps R6870-01 50 preps September 2017 Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4 Preparing Reagents...5 Yeast RNA Protocol...6
More informationEZ-10 SPIN COLUMN HANDBOOK
EZ-0 SPIN COLUMN HANDBOOK EZ-0 Spin Column Plasmid DNA Mini Kit EZ-0 Spin Column PCR Products Purification Kit EZ-0 Spin Column DNA Gel Extraction Kit Version 20.0 Rev 3/23/205 ISO900 Certified 20 Konrad
More informationE.Z.N.A. FFPE RNA Kit. R preps R preps R preps
E.Z.N.A. FFPE RNA Kit R6954-00 5 preps R6954-01 50 preps R6954-02 200 preps January 2015 E.Z.N.A. FFPE RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationInnovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps.
INSTRUCTION MANUAL ZR BAC DNA Miniprep Kit Catalog Nos. D4048 & D4049 Highlights For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion,
More informationInnovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps.
INSTRUCTION MANUAL ZR BAC DNA Miniprep Kit Catalog Nos. D4048 & D4049 Highlights For spin column purification of endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, endonuclease digestion,
More informationTIANprep Yeast Plasmid Kit
TIANprep Yeast Plasmid Kit For fast and convenient purification of plasmid DNA from yeast www.tiangen.com/en DP130227 TIANprep Yeast Plasmid Kit Kit Contents Storage (Spin Column) Cat.no. DP112 Contents
More informationStorage and Stability
Contents Introduction....................................................... 2 Kit Contents...................................................... 3 Before Starting....................................................
More informationBiospin Plasmid DNA Maxi Extraction Kit
Kit Components Biospin Plasmid DNA Maxi Extraction Kit Cat# BSC01S1C BSC01M1C Components 10Tests 25Tests Balance Buffer 20ml 50ml Resuspension Buffer 100ml 250ml Lysis Buffer 100ml 250ml Neutralization
More informationBioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: Fax:
Bioneer Corporation 8-11,Munpyeongseo-ro, Daedeok-gu, Daejeon 34302, Republic of Korea Tel: +82-42-930-8777 Fax: +82-42-930-8688 Email: sales@bioneer.co.kr www.bioneer.co.kr Safety Warnings and Precautions
More informationE.Z.N.A. mirna Kit. R preps R preps R preps
E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationSoil DNA Extraction Kit
Instruction Manual Ver. 09.23.16 For Research Use Only Soil DNA Extraction Kit Advantages IB47800 (4 Preparation Sample Kit) IB47801 (50 Preparation Kit) IB47802 (100 Preparation Kit) Sample: 250-500 mg
More informationZyppy -96 Plasmid MagBead Miniprep Catalog Nos. D4100, D4101, & D4102
INSTRUCTION MANUAL Zyppy -96 Plasmid MagBead Miniprep Catalog Nos. D4100, D4101, & D4102 Highlights Innovative magnetic bead technology for automated and manual applications. Fastest, high-throughput (96-well),
More informationFosmidMAX DNA Purification Kit
Cat. No. FMAX046 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 204 10/2012 1 EPILIT204 Rev. A
More informationInnovative centrifugation based procedure omits conventional cell pelleting and re-suspension steps.
INSTRUCTION MANUAL Zyppy -96 Plasmid Miniprep Catalog Nos. D4041, D4042, & D4043 Highlights Innovative centrifugation based procedure omits conventional cell pelleting and re-suspension steps. The fastest
More informationBACMAX DNA Purification Kit
Cat. No. BMAX044 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 212 10/2012 1 EPILIT212 Rev. A
More informationE.Z.N.A. Plasmid DNA Mini Kit I
E.Z.N.A. Plasmid DNA Mini Kit I D6942-00 5 preps Q - Spin D6942-01 50 preps Q - Spin D6942-02 200 preps Q - Spin D6943-00 5 preps V - Spin D6943-01 50 preps V - Spin D6943-02 200 preps V - Spin E.Z.N.A.
More informationmdi Quanta Giga Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)
Quanta Giga Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial +91-171-2699221,
More informationE.Z.N.A. Plasmid DNA Mini Kit I
E.Z.N.A. Plasmid DNA Mini Kit I D6942-00 5 preps Q - Spin D6942-01 50 preps Q - Spin D6942-02 200 preps Q - Spin D6943-00 5 preps V - Spin D6943-01 50 preps V - Spin D6943-02 200 preps V - Spin E.Z.N.A.
More informationXIT Genomic DNA from Cells
464PR G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name XIT Genomic DNA from Cells For the Isolation of Genomic DNA from Cultured Cells (Cat.
More informationProduct # Specifications. Kit Specifications Column Binding Capacity 25 µg
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18000 Product Insert The BAC DNA
More informationE.Z.N.A. Bacterial RNA Kit. R preps R preps
E.Z.N.A. Bacterial RNA Kit R6950-00 5 preps R6950-01 50 preps July 2017 E.Z.N.A. Bacterial RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4
More informationFast Ion. Plasmid Midi Advanced Kit (incl. Thimble) Plasmid Midi Advanced Kit. Protocol Book. Ion Exchange-High Yield, High Purity, Transfection-grade
Fast Ion Plasmid Midi Advanced Kit (incl. Thimble) Ion Exchange-High Yield, High Purity, Transfection-grade Plasmid Midi Advanced Kit Ion Exchange-High Yield, High Purity, Transfection-grade Protocol Book
More informationml recombinant E. coli cultures (at a density of A 600 units per ml)
for purification of plasmid DNA, from bacterial cultures Cat. No. 1 754 777 (50 purifications) Cat. No. 1 754 785 (50 purifications) Principle Alkaline lysis releases plasmid DNA from bacteria and RNase
More informationPureLink HiPure Plasmid DNA Purification Kits
USER GUIDE PureLink HiPure Plasmid DNA Purification Kits For mini, midi, and maxi preparation of plasmid DNA Catalog Numbers Revision B.0 Publication Number MAN0000486 K2100-02, K2100-03, K2100-04, K2100-05,
More informationPresto Soil DNA Extraction Kit
Instruction Manual Ver. 02.23.17 For Research Use Only Presto Soil DNA Extraction Kit Advantages SLD004 (4 Preparation Sample Kit) SLD050 (50 Preparation Kit) SLD100 (100 Preparation Kit) Sample: 250-500
More informationmdi Endotoxin Free Quanta Mega Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)
Endotoxin Free Quanta Mega Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:
More informationTotal RNA Purification Kit. Cat. #.: TR01 / TR Size : 50 / 150 Reactions Store at RT For research use only
Total RNA Purification Kit Cat. #.: TR01 / TR01-150 Size : 50 / 150 Reactions Store at RT For research use only 1 Description: The Total RNA Purification Kit provides a rapid, simple and effective approach
More informationGenomic DNA Extraction kit (Cultured Cell) 2.0
Genomic DNA Extraction kit (Cultured Cell) 2.0 Ver. Q0307 No part of these protocols may be reproduced in any form or by any mean, transmitted, or translated into a machine language without the permission
More informationmdi Endotoxin Free Quanta Maxi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)
Endotoxin Free Quanta Maxi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax:
More informationAurum Plasmid Midi Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510)
Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 Aurum Plasmid Midi Kit Instruction Manual For technical service, call your local Bio-Rad office, or
More informationE.Z.N.A. Stool DNA Kit. D preps D preps D preps
E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps April 2013 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage
More informationE.Z.N.A. Plasmid DNA Midi Kit
E.Z.N.A. Plasmid DNA Midi Kit D6904-00 2 preps D6904-03 25 preps D6904-04 100 preps E.Z.N.A. Plasmid DNA Maxi Kit D6922-00 2 preps D6922-01 5 preps D6922-02 20 preps D6922-04 100 preps July 2015 E.Z.N.A.
More informationFavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit. User Manual
TM FavorPrep Blood / Cultured Cell Genomic DNA Extraction Maxi Kit User Manual Cat. No.: FABGK 003 (10 Preps) FABGK 003-1 (24 Preps) For Research Use Only v.1005 Introduction TM FavorPrep Genomic DNA Extraction
More informationBioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only.
Note: for laboratory research use only. Endotoxin-free Plasmid DNA Mini-preparation Kit (Spin-column) Cat. # DP2601 (20 preps) DP2602 (50 preps) BioTeke Corporation 1 I. Kit Content Storage and Stability
More informationmdi Quanta Midi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. Membrane Technologies 21, Industrial Area, Ambala Cantt (INDIA)
Quanta Midi Kit User Guide ADVANCED MICRODEVICES PVT. LTD. 21, Industrial Area, Ambala Cantt - 133001 (INDIA) ADVANCED Tel: 0171-2699290, MICRODEVICES 2699471, 2699274 PVT. LTD. 21, Fax: Industrial +91-171-2699221,
More informationMag-Bind RX Plasmid 96 Kit. Table of Contents
Mag-Bind RX Plasmid 96 Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Mag-Bind RX Plasmid 96 Protocol...5 Troubleshooting Guide...8 Manual Revision:
More informationPlasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions
Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296
More informationTIANamp Yeast DNA Kit
TIANamp Yeast DNA Kit For isolation of genomic DNA from yeast cells www.tiangen.com/en DP121221 TIANamp Yeast DNA Kit Kit Contents (Spin Column) Cat. no. DP307 Contents Buffer GA Buffer GB Buffer GD Buffer
More informationMaximum Yield Mini System. Protocol Book YPD100 // YPD300. Ver
HiYield Plasmid Kit Mini Maximum Yield Mini System Protocol Book YPD100 // YPD300 Ver. 2017-1 Precautions I) Handling Requirements Do not use a kit after its expiration date has passed. Some reagents
More informationE.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps
E.Z.N.A. Yeast Plasmid Mini Kit D3376-00 5 preps D3376-01 50 preps November 2015 E.Z.N.A. Yeast Plasmid Mini Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing
More informationSPEEDTOOLS PLASMID DNA PURIFICATION KIT
Manufactured by: BIOTOOLS B&M Labs, S.A. Valle de Tobalina - 52 - Nave 39 28021 Madrid Spain Tel. (34) 91 710 00 74 Fax (34) 93 843 78 84 e-mail: info@biotools.eu www.biotools.eu SPEEDTOOLS PLASMID DNA
More informationUltraClean Midi Plasmid Prep Kit
UltraClean Midi Plasmid Prep Kit Catalog No. Quantity 12700-20 20 Preps Instruction Manual Please recycle Version: 05232014 1 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4
More informationTotal RNA Miniprep Purification Kit. Cat.# :TR01/TR Size : 50/150 Reactions Store at RT
Total RNA Miniprep Purification Kit Cat.# :TR01/TR01-150 Size : 50/150 Reactions Store at RT 1 Description: The Total RNA Miniprep Purification Kit provides a rapid, simple and effective approach to isolate
More informationINDEX INDEX 0 KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 IMPORTANT NOTES 2 EUROGOLD TOTAL RNA ISOLATION PROTOCOL 2 DNA CONTAMINATION 5
INDEX INDEX 0 KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 IMPORTANT NOTES 2 EUROGOLD TOTAL RNA ISOLATION PROTOCOL 2 DNA CONTAMINATION 5 QUANTITATION AND STORAGE OF RNA 5 RNA QUALITY 5 TROUBLESHOOTING
More informationPlasmid DNA 96-Well Kit
Cat. no. EM21 Version: 1.2017 Plasmid DNA 96-Well Kit EXTRACTME is a registered trademark of BLIRT S.A. www.blirt.eu Cat. no. EM21 I. INTENDED USE The EXTRACTME PLASMID DNA 96-WELL KIT is designed for
More informationPlant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 58200
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant DNA Isolation Kit (Magnetic Bead System) 50 Preps Product
More informationThermo Scientific GeneJET Endo-free Plasmid Maxiprep Kit
PRODUCT INFORMATION Thermo Scientific GeneJET Endo-free Plasmid Maxiprep Kit #K0861 Lot Expiry Date _ H www.thermoscientific.com/onebio CERTIFICATE OF ANALYSIS Thermo Scientific GeneJET Endo-free Plasmid
More informationTable of Contents. Page 1. Endofree Plasmid Mega 3 Kit. Biomiga EZgene TM
Table of Contents Contents.. 1 Introduction. 2 Important Notes... 2 Storage and Stability... 5 Before Starting 5 Kit Contents 6 Safety Information. 6 EZgene TM EndoFree Plasmid ezfilter Megaprep 3 Protocol..
More informationGene-Spin DNA Purification Kit - V3
Protech Technology 波仕特生物科技 Gene-Spin 1-4-3 DNA Purification Kit - V3 (For PCR/DNA clean-up & Gel Extraction) Innovative Tools for Nucleic Acid Purification This Kit is for research purposes only. Not for
More informationTable of Contents 1 Introduction 2 Important Notes 2 Storage and Stability 4 Before Starting 4. Important: 4. Materials supplied by users: 5
Table of Contents Table of Contents 1 Introduction 2 Important Notes 2 Storage and Stability 4 Before Starting 4 Important: 4 Materials supplied by users: 5 Kit Contents 5 Safety Information 6 EZgene TM
More information