Buffered Salt Solutions

Transcription

1 Dulbecco s Phosphate Buffered Saline () 27 Phosphate Buffered Saline (PBS) 28 Hanks Balanced Salt Solution () 28 25

2 Introduction Physiochemical Properties Basal salt solutions undergo testing to determine ph and osmolality according to specific protocols which are in line with the National Institute of Standards and Technology. Biological Tests Corning cellgro prides itself on the ability to guarantee low levels of endotoxin, the absence of mycoplasma, and an absence of contamination. Endotoxin: Buffered salt solutions are tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Assay, following guidelines outlined by the manufacturer and the FDA (1987). For release, each lot of buffered salt solutions must have an endotoxin level of 0.25 EU/mL. The majority of Corning cellgro s liquid media are at or below our lowest detectable level today ( EU/mL). Powder products must have an endotoxin level of 0.25 EU/mL when reconstituted to formula weight with low-endotoxin water. Sterility: Corning cellgro selects a number of samples from each production lot to test using the membrane filtration technique for 14 days, in accordance with USP xxiv Chapters <71> and <1211>. Mycoplasma: Buffered salt solution products are tested for mycoplasma off-site using large-volume method described by Kern and Barile in Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques, M.F. Barile and G.J. McGarrity (1983) in Methods of Mycoplasmology, Vol. 11,5. Razin and J.G. Tulley, eds., New York: Academic Press, Visit for additional product and technical information.

3 Dulbecco s Phosphate Buffered Saline () Dulbecco s Phosphate Buffered Saline () is a buffer solution which can be used to maintain cell culture media in the physiological ph range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time. Contains Calcium Magnesium Potassium Chloride Dulbecco s Phosphate Buffered Saline, 10x With calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 18 Months Dulbecco s Phosphate Buffered Saline, 10x Without calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 18 Months Dulbecco s Phosphate Buffered Saline, 1x With calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Dulbecco s Phosphate Buffered Saline, 1x Without calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months LX 10 L 1 15ºC to 30ºC 2ºC to 30ºC 36 Months LB 20 L 1 15ºC to 30ºC 2ºC to 30ºC 36 Months Dulbecco s Phosphate Buffered Saline, Powder Without calcium and magnesium PB 10 L 1 15ºC to 30ºC 2ºC to 8ºC 36 Months PC 50 L 1 15ºC to 30ºC 2ºC to 8ºC 36 Months 27

4 Phosphate Buffered Saline (PBS) Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate. PBS helps to maintain a constant ph and is isotonic and non-toxic to cells. Phosphate Buffered Saline, 1x Without calcium and magnesium ph 7.4 ± CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 24 Months CM 1 L 1 15ºC to 30ºC 2ºC to 30ºC 24 Months Phosphate Buffered Saline, 10x Without calcium and magnesium ph 7.4 ± CM 1 L 6 15ºC to 30ºC 15ºC to 30ºC 24 Months Hanks Balanced Salt Solution () Hanks Balanced Salt Solution () is a buffer used to maintain a physiological ph for cells maintained in non-co 2 atmospheric conditions. Contains Calcium Magnesium Phenol red Sodium Bicarbonate Hanks Balanced Salt Solution, 10x Without sodium bicarbonate, calcium, and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Hanks Balanced Salt Solution, 10x With calcium and magnesium, without phenol red and sodium bicarbonate CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months 28 Visit for additional product and technical information.

5 Hanks Balanced Salt Solution, 1x With calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Hanks Balanced Salt Solution, 1x With calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Hanks Balanced Salt Solution, 1x Without calcium and magnesium CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Hanks Balanced Salt Solution, 1x Without calcium, magnesium, and phenol red CV 500 ml 6 15ºC to 30ºC 2ºC to 30ºC 36 Months CM 1 L 6 15ºC to 30ºC 2ºC to 30ºC 36 Months Hanks Balanced Salt Solution, Powder Without sodium bicarbonate, calcium and magnesium PB 10 L 1 15ºC to 30ºC 2ºC to 8ºC 36 Months Hanks Balanced Salt Solution, Powder Without sodium bicarbonate, calcium, magnesium, and phenol red PB 10 L 1 15ºC to 30ºC 2ºC to 8ºC 36 Months 29

6 , PBS, and TBS Formulation Cat. No Description 10 x, Powder PBS PBS Volume g/l g/l g/l g/l g/l g/l g/l g/l Inorganic Salts CaCl 2 (anhydrous) KCl KH 2 PO MgCl 2 6H 2 O TBS NaC l Na 2 HPO 4 (anhydrous) Na 2 HPO 4 7H 2 O Tris Ultrapure Specifications ph (before adjustment) ± Final ph 4.4 ± ± ± ± ± ± 1:10 Osmolality (mosm) 276 ± 1:10 Grams of powder required to prepare 1L (1x solution) 276 ± 1:10 1: ± ± ± ± Visit for additional product and technical information.

7 Hank s Balanced Salt Solution () Formulation Cat. No Description Powder Volume g/l g/l g/l g/l g/l g/l g/l g/l Inorganic Salts CaCl 2 (anhydrous) KCl KH 2 PO MgSO 4 (anhydrous) NaC l Powder Na 2 HPO 4 (anhydrous) NaHCO Other D-Glucose Phenol red, Na Add NaHCO 3 Powder (g/l) % solution (ml/l) Specifications ph (before adjustment) ± 0.5 Final ph 6.0 ± 1.0 Osmolality (mosm/kg) Grams of powder required to prepare 1L (1x solution) 275 ± 30 (1:10) 4.75 ± ± 30 (1:10) 7.25 ± ± ± ± ± ± ± ± ± ± ± ±

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