New Brunswick fermentors and bioreactors for research through production
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1 Culture success New Brunswick fermentors and bioreactors for research through production
2 New Brunswick fermentors & bioreactors for all your culture needs New Brunswick fermentors and bioreactors by Eppendorf offer: > > A wide range of fermentor and bioreactor products, both stirred tank and packed-bed systems > > 1-3,000 Liter capacities, for research, pilot scale or cgmpcompliant production > > Industry-standard and proprietary impellers to meet virtually any culture need > > Set-up and start-up assistance, a comprehensive warranty, as well as a wide range of optional ancillary equipment, product training, validation and support packages > > The combined resources, product portfolios, experience and applications support of New Brunswick and Eppendorf working to offer you a complete process solution, tailored to meet your exact needs Whether you prefer the convenience of a single-use bioreactor, the flexibility of an autoclavable system, or the robustness of a sterilizable-in-place () production-scale reactor, we have a system right for you.
3 Volume Sterilization Method 1 Bacteria/Yeast/Fungi Plant/Algae Mammalian/Animal Insect Interchangeable Vessels Controller 2 Touchscreen Controller Air Flow Control 3 Mixing Options Validatable CelliGen BLU Single-Use Bioreactor TOTAL L WORKING L S 3/4 BioFlo / CelliGen 115 /Bioreactor TOTAL L WORKING L A BioFlo 310 TOTAL L WORKING L A CelliGen 310 Bioreactor TOTAL L WORKING L A BioFlo 415 TOTAL L WORKING L BioFlo 510 TOTAL L WORKING L / PLC CelliGen 510 Bioreactor TOTAL L WORKING L / PLC BioFlo 610 TOTAL L WORKING L 1/2 BioFlo Pro TOTAL L WORKING L PLC 2 CelliGen Pro Bioreactor TOTAL L WORKING L PLC 4 1 Sterilization Methods: S=Single-Use, A=Autoclavable, =Sterilizable-in-Place 2 Controllers: =Reactor Process Controller, PLC=Programmable Logic Controller 3 Air Flow Controllers: R=Rotameter, =Thermal Mass Flow Controller
4 Easy to use exceedingly versatile Single-use and autoclavable fermentors and bioreactors CelliGen BLU Single-Use Bioreactor 5.0, 14.0 & 50.0 L Total Volume BioFlo /CelliGen 115 Autoclavable /Bioreactor 1.3, 3.0, 7.5, & 14.0 L Total Volume > > Designed for high-density cell culture in research or production applications > > Rigid-walled, single-use stirred-tank vessel for proven performance and scalability > > Eliminates vessel autoclaving and cleaning > > All vessel components in product contact are made of USP Class VI materials and tested for leachables and extractables > > Available 5.0 L packed-bed vessel: first single-use vessel to feature New Brunswick s proprietary packed-bed impeller > > Features New Brunswick s intuitive control > > Unique non-invasive ph and DO technology > > Adaptor kits are now offered to enable your existing New Brunswick, Applikon and Sartorius controller to be used with CelliGen BLU single-use vessels > > Versatile entry-level autoclavable system > > Pre-programmed with both fermentation and cell culture operating modes for flexibility > > Ability to control up to three independent reactors from a single easy-to-use touchscreen > > Features New Brunswick s intuitive control > > 8 Interchangeable vessel options > > Precise temperature control provided through heat blanketed vessel (shown above) or water jacketed vessel (shown on the cover) > > Customizable gas flow and mixing options > > Available in pre-defined packages for easy ordering, or fully configurable systems to meet specific needs
5 BioFlo 310 & CelliGen 310 Bioreactor Autoclavable Benchtop Systems 2.5, 5.0, 7.5 & 14.0 L Total Volume Unique Impellers and Fibra-Cel Disk Options Enhance Growth and Productivity > > Premium autoclavable system, designed for complete versatility and full cgmp compatibility to meet your research and production needs > > Ideal for the growth of virtually any cell line in aerobic or anaerobic environments > > Features New Brunswick s intuitive control > > Fully customizable trend screen cascades for data logging and localized process control > > Multiple impellers, gas flow options, redundant probes and more allow ready customization New Brunswick offers a diverse a range of specialized impellers including: 1. Spin Filter (shown with marine blade impeller) 2. Cell-Lift Impeller 3. Patented Packed-Bed Basket Impeller with draft tube for use with Fibra-Cel disks (complete impeller assembly shown top right) 4. Pitched Blade Impeller 5. Marine Blade Impeller > > Rushton-type Impeller (not shown) Fibra-Cel disks are a solid support material used with New Brunswick s packed-bed bioreactors for growth of mammalian, animal and insect cells, when secreted proteins are desired. Key advantages: > > Cell densities averaging 10 times higher than traditional microcarriers, due to protection from shear forces > > Higher mass transfer of nutrients and oxygen > > Low pressure drop across the bed for efficient scale-up > > Higher surface-to-volume ratios
6 Industry standards meet flexibility Sterilizable-in-place fermentors and bioreactors BioFlo 415 Benchtop System - Needs No Steam 7.0, 14.0 & 19.5 L Total Volume BioFlo 510 & CelliGen 510 Bioreactor Benchtop System 19.5 & 40 L Total Volume > > Unique automated process utilizes only a water supply and the system s built-in electric heat blanket for sterilization - no external steam supply needed > > Eliminates the need for transferring vessels to the autoclave for sterilization > > Large 38 cm (15 in) fully integrated touchscreen > > Features New Brunswick s intuitive control > > Fully customizable trend screen for data logging > > User-definable cascades for localized process control > > ASME rated pressure vessel > > benchtop reactors designed to meet the needs of R&D through production > > Modular design allows for complete system flexibility > > Add or remove components pre- or post-delivery > > Multiple impeller and gas flow options > > Optional Allen Bradley PLC > > Features New Brunswick s intuitive control > > Integrated load cell for online vessel volume monitoring > > Fully automated sequence for sterilization > > ASME rated pressure vessel
7 BioFlo 610 System with Integrated Mobile Skid 65 & 125 L Total Volume BioFlo Pro & CelliGen Pro Bioreactor Modular Customizable systems BioFlo Pro: L Total Volume CelliGen Pro: L Total Volume > > Mobile skid simplifies transport > > Pilot production system without the traditional cost or footprint > > Modular design allows for complete system flexibility > > Features New Brunswick s intuitive control > > Fully integrated load cell for online vessel volume monitoring > > ASME rated pressure vessel > > Full adherence to cgmp and GaMP guidelines > > Customizable pre- or post-delivery > > Multiple validation package offerings > > Open piping skid allows for easy accessibility > > NEMA-4 rated control cabinet with industry-standard Allen Bradley PLC > > Flush mounted vessel ports for enhanced vessel design and drainability > > Multiple impeller and gas flow options > > ASME rated pressure vessel
8 Advanced control made simple Controller Easy to Use. Lightning-Fast Navigation Between Screens New Brunswick s advanced Reactor Process Control () is provided as standard on all systems except the Pro line (which uses an Allen Bradley PLC), simplifying transition between systems during scale-up. Control screens are easy to understand and use. > > Pre-defined drop-down selections, radio buttons and tabs help you to quickly set up your process > > Quick links at the bottom of each screen make it one-touch simple to navigate between Set-Up, Calibration, Cascade, Pumps and Summary displays Summary screen displays all of your critical process values on one screen for easy monitoring. View the setpoint, present value (PV), current percent output and control mode for each loop, as well as cascades you ve programmed and unit of measure Setup screens let you manage connections and operating modes for each vessel in your process BioCommand Software Adds Advanced Control and Monitoring Capability New Brunswick s optional BioCommand PC-based automates data logging, provides remote monitoring and control capabilities, and is OPC-compliant, allowing data from other OPC-compatible devices to be integrated into your control strategies. BioCommand is offered in choice of three packages. > > Track and Trend provides monitoring and historical record keeping, with control of setpoints, alarms and trend displays > > Batch Control helps you optimize your process. It adds equipment lock-out features for security, and recipe features such as programming and a customizable synoptic display > > For FDA-validated processes choose Batch Control Plus. It adds three levels of security (operator, supervisor and administrator), event logs, audit trails and a database structure to help comply with 21 CFR Part 11 guidelines Your local distributor: Eppendorf AG Hamburg Germany Tel: Application Support support@eppendorf.com Eppendorf North America, Inc. USA Tel: Toll free phone: menu option 2 techserv@eppendorf.com Eppendorf is a registered trademark and New Brunswick is a trademark of Eppendrf AG, Germany. BioFlo, CelliGen and BioCommand are registered trademarks of New Brunswick Scientific Co., Inc., USA. All rights reserved, including graphics and photos. Copyright 2012 by Eppendorf. Order no. B-1001a
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