OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : :51:53 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Rabies Address of laboratory: Liuying Xi Road 666 Jingyue Economic Development Zone Changchun CHINA (PEOPLES REP. OF) Tel.: Fax: address: changchun_tu@hotmail.com website: Name (including Title) of Head of Laboratory (Responsible Official): Prof. Changchun Tu Name (including Title and Position) of OIE Reference Expert: Prof. Changchun Tu Which of the following defines your laboratory? Check all that apply: Academic OIE Reference Laboratory Reports Activities,

2 ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Fluorescent antibody virus neutralisation (FAVN) 79 (dog and cat) 56 (giant panda) -- Immunochromatographic test strip (ICTS) for antibody Direct diagnostic tests Nationally Internationally Fluorescent antibody test (FAT) 9 (suspected dogs) -- Cell culture inoculation test (RTCIT) 9 (suspected dogs) -- Reverse transcription-pcr (RT-PCR) 499 (samples from apparently healthy dogs for pathogen surveillance, including 454 brain tissues and 45 saliva swabs) 83 (samples from bat brain tissues for wildlife surveillance) -- Real time TaqMan RT-PCR (FQ-PCR) 499 (samples from apparently healthy dogs for pathogen surveillance, including 454 brain tissues and 45 saliva swabs) 83 (samples from bat brain tissues for wildlife surveillance) -- G gene and N gene Sequencing 8 -- ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens 2 OIE Reference Laboratory Reports Activities, 2013

3 or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients RT-nPCR kit RT-nPCR test Produced 12 kits each (50 samples/kit) Africa Americas Asia and Pacific Europe Middle East FQ-PCR kit FQ-PCR test Produced 12 kits each (50 samples/kit) Africa Americas Asia and Pacific Europe Middle East ICTS for virus ICTS test Produced 800 strips Africa Americas Asia and Pacific Europe Middle East ICTS for serum ICTS test Produced 2500 strips Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? OIE Reference Laboratory Reports Activities,

4 ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Immunochromatographic test strip (ICTS) for antibody Wang et al. (2010). A rapid immunochromatographic test strip for detecting rabies virus antibody. Journal of Virological Methods, 170 (2010) ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided VIETNAM Improve laboratory techniques in diagnoses of animal rabies Meeting in local laboratories VIETNAM Improve their real-time PCR method Remote by CHINA (PEOPLE'S REP. OF) Establish local rabies diagnosis laboratory in Macao Remote by ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2013

5 ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 Wenwu Yin,Jie Dong,Changchun Tu,John Edwards,Fusheng Guo,Hang Zhou,Hongjie Yu,Sirenda Vong.(2013) Challenges and needs for China to eliminate rabies. Infect Dis Poverty. Oct 2; 2(1):23. Xianghong Xue, Xuexing Zheng, Weiwei Gai, Ling Li, Jinzhu Ma, et al. Development of a novel method for detection of rabies virus neutralizing antibody. Chin J Biologicals, 2013,26(8): Xianghong Xue, Xuexing Zheng, Weiwei Gai, Hongru Liang, Jinzhu Ma, et al. Sequencing the complete genome of rabies virus CVS-11 strain and constructing its full-length infectious cdna clone. Acta Microbiologica Sinica, 2013, 53(4): Lili Zhao, Pingsen Zhao, Yinglin Qi, Yujiao Yang, Yongkun Zhao, et al. Expression of the mir-200 family in the brain of mice infected with the rabies virus and prediction of mir-200 family target genes. Journal of Pathogen Biology, 2013,8(3): Pingsen Zhao, Yujiao Yang, Hao Feng, Lili Zhao, Junling Qin, Tao Zhang, et al. Global gene expression changes in BV2 microglial cell line during rabies virus infection. Infection, Genetics and Evolution. 2013, 20, b) International conferences: 2 Changchun Tu. Global Scenario of Rabies. Inception Meeting of the OIE/JTF Project for Controlling Zoonoses in Asia Under One Health Concept th December Tokyo, Japan Changchun Tu. Laboratory component for Regional Rabies Control Initiatives. The 2013 Regional Animal Health Laboratory Technical Advisory Group (Lab-TAG) meeting th October 2013, Yogyakarta, Indonesia. c) National conferences: 1 Ye Feng, Changchun Tu. The construction on veterinary diagnostic laboratory Rabies Conference in China, 11-12nd May 2013, Chengdu, China. d) Other: (Provide website address or link to appropriate information) 2 The 2013 report of rabies work to Chinese Ministry of Agriculture. Report of a dog rabies in Inner Mongolia to Chinese Ministry of Agriculture. OIE Reference Laboratory Reports Activities,

6 ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 2 c) Hands-on training courses: 0 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country b Macao, China 2 ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO and the Quality Management System of CNAS are adopted in our laboratory for quality control and we are preparing for the certification next year. 16. Is your laboratory accredited by an international accreditation body? 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 6 OIE Reference Laboratory Reports Activities, 2013

7 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Inception Meeting of the OIE/JTF Project for Controlling Zoonoses in Asia Under One Health Concept 12/2013 Tokyo, Japan speaker Global scenario of rabies The 2013 Regional Animal Health Laboratory Technical Advisory Group (Lab-TAG) meeting 10/2013 Yogyakarta, Indonesia speaker Laboratory component for regional rabies control initiatives Third Rabies Technical and Advisory Board Meeting UNTGH on Human-animal Interface 12/2013 Beijing, China short communication ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure OIE Reference Laboratory Reports Activities,

8 equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) FAO/OIE rabies mission Vietnam Provide scientific and technical advice to Vietnam to help with the implementation control measures and diagnosis of the disease at the animal reservoir, as well as epidemiologically assess the risk of transboundary spread of the disease 25. Additional comments regarding your report: Obtaining the official import permit is still the biggest obstacle for our OIE RLs in China to receive animal specimens of designated disease from other OIE member countries, which obviously prevent us from fulfilling our duty as OIE RL. This is the reason that we are difficult to provide diagnostic assistance to other countries, as well as to participate in international inter-laboratory proficiency tests. 8 OIE Reference Laboratory Reports Activities, 2013