Primary & Stem Cell Research

Transcription

1 Total Service for Primary & Stem Cell Research * Human Dental Cell Culture System * Human Skin and Hair Cell Culture System Crown Root Enamel Dentine Pulp Gum Cementum Bone Blood Vessel Nerve Dermal papilla Sebaceous gland Bulge Hair shaft Root sheet area Matrix cells PDL SHED ORS HDP HDF HDP, AP Wharton s jelly HEK P63 HEM HEK HEM, TRP1 * Endothelial Cell Culture system (HUVEC and SMC) Chondrogenesis Bone marrow Human MSC Culture System Fat Osteogenesis Adipogenesis For Better Quality For Better Service For Better Value Homepage: info@cefobio.com

2 We develop and support your research with primary stem cells CEFO Bio is a company providing primary and stem cells from bench to bedside; normal and diseased human tissue-derived cells, pluripotent stem cells, stem cell-derived differentiated cells and primary cancer tissues. We supply a broad range of cells and culture medium optimized from our technical expertise in laboratory and from our knowledge of stem cell niche environment. Primary Cells Pluripotent Stem Cell System Tissue-derived MSC Skin and Hair Cell Culture System Dental Tissue-derived Cells Endothelial Cell Culture System Disease Cells Animal Cells Reagents Culture Medium 3D System Primary Cell Isolation System Contract Research Service Characterization of pluripotent stem cells Teratoma assay/eb formation Establishment of primary and stem cell lines Test for efficacy, toxicity and delivery using skin model Angiogenic sprouting assay Cancer invasion assay / Soft agar colony formation assay Test of anti-cancer drug Provision of cells on 96-well plates for high throughput Hands-on workshop for stem cell culture Imaging System We meet Bioethics and Safety Act in human tissue preparation

3 Screening Model and Service 3D Culture Matured Osteoblast 3D Differentiation Angiogenesis Model For research and drug screening Scaffold culture Synthetic Bio-gel Feeder free culture of pluripotent stem cell and provide mass culture product * 3D Skin & Hair Structure * 96 well culture service for multi-screening Red Dot: QD2 3D Hair Structure Pluripotent stem cell CEFO Skin 3D Embryonic body Red Dot: QD2 Pharmaceutical and Diagnosis and Cosmetic application

4 Human Mesenchymal Stem Cell Culture System Mesenchymal Stem Cells (MSCs) is useful cell source due to its clinical applicability for regeneration of organ and for in vitro testing such as drug screening and toxicity, efficacy, etc. CEFO provides MSC derived from various tissues such as bone marrow, adipose tissue, umbilical cord. Characterization of MSC BMMSC ADMSC UCMSC Surface markers CD31(-) CD73(+) CD105(+) CD31(-) CD73(+) CD105(+) CD146(+) CD31(-) CD73(+) CD105(+) Differentiation capacity Osteogenesis (Alizarin Red Staining) Adipogenesis (Oil Red O Staining) Chondrogenesis (Safranin O Staining) Phase Contrast Image Bone Marrow derived MSC : BMMSC Umbilical Cord derived MSC : UCMSC Adipose Tissue derived MSC : ADMSC Chondrogenesis Osteogenesis Adipogenesis Contract research service - Toxicity - Tumorigenesis - Migration - 96-well plate CD31 CD73 CD105 We provide High Quality Cells, optimized Culture Medium, Freezing Medium and Differentiation Kit

5 Human Dental Cell Culture System PDL Human dental tissue-derived cells are valuable due to their applicability for regeneration of dental tissue. CEFO provides various dental stroma cells as listed. SHED - DPSC : Dental Pulp Stem Cells - SHED : Stem Cells from Human Exfoliated Deciduous Teeth - STDPSC : Dental Pulp Stem Cells isolated from Supernumerary Teeth - PDL : Periodontal Ligament Cells We provide High Quality Cells, Culture Medium, Freezing Medium, Microbial Protecting /Cure system and Differentiation Kit Crown Root Enamel Dentine Pulp Gum Cementum Bone Blood Vessel Nerve Characterization of Dental Tissue-derived Cells Periodontal Ligament Cell (PDL) Dental Pulp Stem Cell (DPSC) Stem cells from Human Exfoliated Deciduous teeth (SHED) Surface markers CD146(+) STRO-1(+ : low expression <12%) CD105(+) STRO-1(+ : low expression <15%) CD105(+) STRO-1(+ : low expression <15%) Differentiation capacity Osteogenesis (Alizarin Red Staining) Adipogenesis (Oil Red O Staining) Chondrogenesis (Safranin O Staining) Phase Contrast Image Ref : Regen Med (2011) 6(6) Application: Research on Oral and Maxillofascial Regeneration and Periodontal Regeneration, ips Derivation Cell Sources

6 Human Skin & Hair Cell Culture System HDF HEK Human Skin-derived and Hair Follicle-derived Cells are valuable due to their applicability to research on Skin and Hair Biology. CEFO provides various Skin and Hair folliclederived Cells as listed. HEM - HDF : Human Dermal Fibroblasts - HEK : Human Epidermal Keratinocytes - HEM : Human Epidermal Melanocytes - HDP : Human hair follicle Dermal Papilla - ORS : Human hair Outer Root Sheet Cells ORS We provide High Quality Cells, Culture Medium, Freezing Medium, Enzyme Neutralizing Solution, 3D skin model, Immune Related Assay HDP, AP CEFO Skin 3D CEFO skin is composed of Human Keratinocytes, Dermal Fibroblasts and either Collagen or Synthetic BioGel. CEFO Skin is highly stratified epidermal layer mimicking real human skin to test - Skin Corrosion - Skin Irritation Culture System We provide - Enzyme solution for HEK - Enzyme Neutralizing Solution - The dual medium for ORS attachment and ORS growth

7 Drug Treatment Control(+) Cell Engineering For Origin (CEFO) Co. Ltd Human Endothelial Cell Culture System HUVEC : Human Umbilical Vein Endothelial Cells HUSMC : Human Umbilical Smooth Muscle Cells Primary human umbilical vein endothelial cells (HUVECs) isolated from the vein of the umbilical cord are cultured in CEFOgro ENDO (CB-END-GM). Primary human umbilical smooth muscle cells (HUSMCs) isolated from vein and artery of the umbilical cord are cultured in CEFOgro SMC (CB-SMC-GM). Applicable for vascular research and drug testing. Characterization of Umbilical Vein-derived Cells Characteristics HUVEC CD31 HUSMC CD31 Tube Formation : F-actin α-sma Morphology Cobblestone appearance We provide Cells, Culture medium, Freezing medium, Angiogenic Cell Model Phase Contrast QD 2 Red F-actin Length measurement We also provide Angiogenic sprouting assay via Contract Research (Efficacy and Toxicity testing)

8 Pluripotent Stem Cell Supports Feeder Cell Service : MEF : Mouse Embryonic Fibroblasts, CF1 HDF : Human Dermal Fibroblasts Feeder Cell Culture System - Culture Medium - Feeder Cell Cycle Arrest Reagent (CCArg) - Cell cycle-arrested Feeder Cells - Service for Provision of Feeder Cells Human Pluripotent Stem Cell Service : hes/ips Cell Culture Start Kit Hands-on-workshop Service for Storage, Characterization, Teratoma, Embryoid Body Contract Research Service Mass culture of Pluripotent Stem Cells PS in 96 well plate EB in 96 well plate ** Special Reagents CCArg : Cell Cycle Arrest reagent for feeder cells in pluripotent stem cells ENS : Enzyme Neutralizing Solution to neutralize the enzymatic activity in Keratinocyte Culture 3D Cell Recovery Solution for harvesting cells from 3D scaffold or gel Primary ENZ : Cell dissociation Enzyme for Primary Culture CEFO-Vive : Cell Storage Solution at 4 or in room temperature. - Guarantee of cell survival to maximum of 5 days at 4. For intermission of experiment - Xeno-Free and animal-free RBC Lysis Buffer QD2 : Quantumn Dot (green, red, blue) for Labeling Cells. High Efficiency and No Toxicity Cytokines: bfgf, EGF, VEGF, HGF, BMP Enzymes: Collagenase I, II, IV

9 Primary & Stem Cell Product List Catalogue No Product Catalogue No Product Pluripotent Stem Cell Culture System Skin and Hair Cell Culture System CB-CF1-001 CF1 Mouse embryonic fibroblast 1vial CB-HDF-001 Human Dermal Fibroblast cells (1vial) CB-CF1-002 CF1 Mouse embryonic fibroblast (2vials/set) CB-HDF-002 Human Dermal Fibroblast cells (T75 1 flask) CB-CF1-003 CF1 Mouse embryonic fibroblast 2 Flasks (T75)/set CB-HDF-003 Human Dermal Fibroblast cells kit I CB-CF1-004 CF1 Mouse embryonic fibroblast Kit I 1 vial, 500 ml GM, CCArg, 10 ml FM CB-HDF-004 Human Dermal Fibroblast cells kit II CB-CF1-005 CF1 Mouse embryonic fibroblast Kit II 2 vials, 500 ml GM, CCArg, 10 ml FM CB-HEK-001 Human Epidermal Keratinocytes (1vial) CB-CF1-006 CF1 Mouse embryonic fibroblast Cell cycle arrested (5vials / 1set) CB-HEK-002 Human Epidermal Keratinocytes (T75 1 flask) CB-ES-001 hesc lysate CB-HEK-003 CB-ES-002 hesc for RNA/DNA purification (dissolved in Trizol) CB-HEK-004 Human Epidermal Keratinocyte Kit I 1 vial, 500 ml GM, 10ml FM, TNS Human Epidermal Keratinocyte Kit II 1 Flask(T25), 500 ml GM, 10ml FM, TNS CB-ES-003 Pluripotent stem cell culture start kit Feeder 1vial, CCArg, PS-GM, MEF-GM CB-MEL-001 Human Epidermal Melanocytes (1vial) CB-ES-004 Pluripotent stem cell culture hands-on workshop CB-MEL-002 Human Epidermal Melanocytes (T75 1 flask) CB-ES-005 Pluripotent stem cell storage service Thawing, Freezing, Culture service CB-MEL-003 Human Epidermal Melanocyte Kit I 1 vial, 500ml GM, 10 ml FM Tissue-derived MSC CB-MEL-004 Human Epidermal Melanocyte Kit II 1 Flask(T75), 500ml GM, 10 ml FM CB-BMMSC-001 Human Bone Marrow-derived MSC : Male, 1vial CB-MEL-005 Human Epidermal Melanocyte Kit III 1 vial, 500ml GM(PMA-free), 10 ml FM CB-BMMSC-002 Human Bone Marrow-derived MSC:Male, T75 1 flask CB-HDP-001 Human Hair Follicle Dermal Papilla cells (1vial) CB-BMMSC-003 Human Bone Marrow-derived MSC Kit I : Male CB-HDP-002 Human Hair Follicle Dermal Papilla cells (T75 1 flask) CB-BMMSC-004 Human Bone Marrow-derived MSC Kit II: Male CB-HDP-003 Human Hair Follicle Dermal Papilla cell Kit I CB-BMMSC-005 Human Bone Marrow-derived MSC : Female, 1vial CB-HDP-004 Human Hair Follicle Dermal Papilla cell Kit II CB-BMMSC-006 Human Bone Marrow-derived MSC Female, T75 1Flask CB-ORS-001 Human Outer Root Sheath cell Kit I 1 Flask (T75), 500 ml GM, TNS CB-BMMSC-007 Human Bone Marrow-derived MSC Kit I : Female CB-ORS-002 Human Outer Root Sheath cell Kit II 1 vial, 500ml GM, Attachment Medium50 ml, TNS CB-BMMSC-008 Human Bone Marrow-derived MSC Kit II : Female Dental Tissue-derived Cells CB-ADMSC-001 Human Adipose tissue-derived MSC 1vial CB-SHED-001 Stem Cells from Human Exfoliated Deciduous Teeth 1 vial CB-ADMSC-002 Human Adipose tissue-derived MSC : T75 1 Flask CB-SHED-002 Stem Cells from Human Exfoliated Deciduous Teeth T75 1 Flask CB-ADMSC-003 Human Adipose tissue-derived MSC Kit I CB-SHED-003 Stem Cells from Human Exfoliated Deciduous Teeth Kit I CB-ADMSC-004 Human Adipose tissue-derived MSC Kit II CB-SHED-004 Stem Cells from Human Exfoliated Deciduous Teeth Kit II 1 Flask(T75), 500 ml GM, 10 ml FM CB-UCMSC-001 Human Umbilical Cord-derived MSC 1vial CB-SDPS-001 Human Supernumerary Teeth Derived DPSC 1vial CB-UCMSC-002 Human Umbilical Cord-derived MSC : T75 1 Flask CB-SDPS-002 Human Supernumerary Teeth Derived DPSC : T75 1 Flask CB-UCMSC-003 Human Umbilical Cord-derived MSC Kit I CB-SDPS-003 Human Supernumerary Teeth Derived DPSC Kit I CB-UCMSC-004 Human Umbilical Cord-derived MSC Kit II CB-SDPS-004 Human Supernumerary Teeth Derived DPSC Kit II

10 Primary & Stem Cell Product List Catalogue No Product Catalogue No Product Endothelial Cell Culture System CB-DPDL-001 Human Deciduous Teeth-derived PDL Cells 1vial CB-HUVEC-001 Human Umbilical Vein Endothelial Cells 1vial CB-DPDL-002 Human Deciduous Teeth-derived PDL Cells : T75 1 Flask CB-HUVEC-002 Human Umbilical Vein Endothelial Cells : T75 1 Flask CB-DPDL-003 Human Deciduous Teeth-derived PDL Cell Kit I CB-HUVEC-003 Human Umbilical Vein Endothelial Cell Kit I CB-DPDL-004 Human Deciduous Teeth-derived PDL Cell Kit II 1 Flask(T75), 500 ml GM, 10 ml FM CB-HUVEC-004 Human Umbilical Vein Endothelial Cell Kit II CB-SPDL-001 Human Supernumerary Teeth-derived PDL Cells 1vial CB-SMC-001 Human umbilical cord-derived smooth muscle Cells 1vial CB-SPDL-002 Human Supernumerary Teeth-derived PDL Cells : T75 1 Flaks CB-SMC-002 Human umbilical cord-derived smooth muscle Cells T75 1Flask CB-SPDL-003 Human Supernumerary Teeth-derived PDL Cells Kit I CB-SMC-003 Human umbilical cord-derived smooth muscle Cell Kit I CB-SPDL-004 Human Supernumerary Teeth-derived PDL Cells Kit II CB-SMC-004 Human umbilical cord-derived smooth muscle Cell Kit II CB-DPSC-001 Human Permanent Teeth-derived Dental Pulp Stem Cells 1vial CB-HDP-DIS Neuronal diseased HDP (Hallevorden-Spatz, Parkinson's, dystonia, autism) CB-DPSC-002 Human Permanent Teeth-derived Dental Pulp Stem Cells T75 1 Flask CB-ORS-DIS Neuronal diseased ORS (Hallevorden-Spatz, Parkinson's, dystonia, autism) CB-DPSC-003 Human Permanent Teeth-derived Dental Pulp Stem Cells Kit I 1vial, 500 ml GM, 10 ml FM CB-PDL-DIS Periodontal disease PDL (Hereditary/multiple loss of permanent teeth, Developmental anomalies) CB-DPSC-004 Human Permanent Teeth-derived Dental Pulp Stem Cells Kit II 1 Flask(T75), 500 ml GM, 10 ml FM * Cells in culture should be ordered at least one week in advance CB-PDL-001 Human Permanent Teeth-derived PDL Cells 1vial * GM: Growth Medium / FM : Freezing Medium CB-PDL-002 Human Permanent Teeth-derived PDL Cells : T75 1 Flask * CCArg: Cell Cycle Arrest reagent CB-PDL-003 * ENS: Enzyme neutralizing solution We meet Bioethics and Safety Act in human tissue preparation CB-PDL-004 Human Permanent Teeth-derived PDL Cells Kit I Human Permanent Teeth-derived PDL Cells Kit II 1 Flask(T75), 500 ml GM, 10 ml FM

11 Primary & Stem Cell Culture Supports Catalogue No Product description Product Name CB-MF-GM Mouse Fibroblast Growth Medium CEFOgro TM MF CB-HF-GM Human Fibroblast Growth Medium CEFOgro TM HF CB-HF-GM02 Human Fibroblast Growth Medium : Phenol Red Free CEFOgro TM HF-II CB-HEK-GM Human Keratinocyte Growth Medium CEFOgro TM HK CB-HEK-GM02 Human Keratinocyte Growth Medium : Phenol Red Free CEFOgro TM HK-II CB-MEL-GM01 Human Epidermal Melanocyte Growth Medium CEFOgro TM MEL CB-MEL-GM02 Human Epidermal Melanocyte Growth Medium (PMA-free) CEFOgro TM MELplus CB-MEL-GM03 Human Epidermal Melanocyte Growth Medium (PMA-free) : Phenol Red Free CEFOgro TM MELplus2 CB-HDP-GM Human Dermal Papilla Growth Medium CEFOgro TM HDP CB-HDP-GM02 Human Dermal Papilla Growth Medium : Phenol Red Free CEFOgro TM HDP-II CB-ORS-GM Human Outer Root Sheath cell Growth Medium CEFOgro TM ORS CB-DPC-GM Human Dental Pulp Cell Growth Medium CEFOgro TM DPC CB-PDL-GM Human Periodontal Ligament Cell Growth Medium CEFOgro TM PDL CB-BMMSC-GM Human Bone Marrow MSC Growth Medium CEFOgro TM BM CB-MSC-GM-XF Human MSC Growth Medium (Xeno-free) CEFOgro TM XF MSC CB-ADMSC-GM Human Adipose Tissue Derived MSC Growth Medium CEFOgro TM ADMSC CB-UCMSC-GM Human Umbilical Cord derived MSC Growth Medium CEFOgro TM UCMSC CB-END-GM Human Endothelial Growth Medium CEFOgro TM ENDO CB-SMC-GM Human Smooth Muscle Cell Growth Medium CEFOgro TM SMC CB-NSC-GM Human Neural Stem Cell Growth Medium CEFOgro TM NSC CB-DM-Neural Human Neural Stem Cell Differentiation Medium CEFOgro TM DM Neuro CB-Astro-GM Human Astrocyte Growth Medium CEFOgro TM Astro CB-ES-GM Human Embryonic Stem Cell Growth Medium CEFOgro TM ES CB-CSC-GM Human Cancer Stem Cell Growth Medium CEFOgro TM CSC CB-CCARG Cell Cycle Arrest Solution CEFO CCArg CB-ENS Enzyme Neutralizing Solution CEFO ENS CB-3D Cell Recovery Cell Recovery solution from 3D scaffold or gel CB-3D Cell Recovery Primary ENZ Cell dissociation Enzyme for Primary culture CEFP ENZ CEFO vive Cell storage solution at 4C or RT CEFO vive RBC Lysis Buffer CEFO RBC Lysis QD2-Green/Red/Blue Quantum dot for labelling cells (green, red and blue available) CEFO QD2 CB-DM-Adipo Human MSC Differentiation Medium (Adipogenesis) CEFOgro TM DM Adipo CB-DM-Osteo Human MSC Differentiation Medium (Osteogenesis) CEFOgro TM DM Osteo CB-DM-Chondro Human MSC Differentiation Medium (Chondrogenesis) CEFOgro TM DM Condro * GM: Growth Medium * FM : Freezing Medium * CCArg: Cell Cycle Arrest reagent * TNS: Enzyme neutralizing solution

12 Contact 세포바이오 : 서울시종로구수송동 ( ) Fax CEFO Co., Ltd 5 th Floor Ujeongguk-ro Jongno-gu Seoul Tel / /info@cefobio.com /