High-Performance Liquid Chromatography

Transcription

1 High-Performance Liquid Chromatography ULTRON VX STR Silica Columns VX / STR Series VX-ODS VX-Octyl VX-SIL STR ODS-II STR ODS-M

2 ULTRON VX-ODS ULTRON VX-ODS columns are reversed phase columns employing high purity silica gel (>99.99%) as a substrate to which octadecyl groups are chemically bonded. In addition, the use of unique end-capping technology has resulted in a high density end-capped packing material with extremely low residual silanol effects. Features 1 Reproducibility between column lots: ULTRON VX-ODS columns are checked using stringent quality control in each manufacturing process. As an example, k' and α values obtained using ULTRON VX-ODS columns display only extremely small differences between lots. ULTRON VX-ODS columns also display excellent reproducibility and stability in other tests between column lots. 2 Pressure resistance: Because ULTRON VX-ODS columns use spherical silica gel, they display excellent pressure resistance, and stable separations can be achieved even at fast mobile phase flow rates. 3 Stereoselectivity, distribution equilibria: Because ULTRON VX-ODS columns utilize ODS monolayers, they display excellent stereoselectivity with respect to sample molecules. Distribution equilibria are reached rapidly when performing gradient elutions and when mobile phases are changed. Physical properties Particle size Pore size Specific surface area Carbon content 5 µm 12 nm 300 m 2 /g 15% Basic compounds Acidic compounds 1 Pyridine 2 Phenol 1 p-hydroxybenzoic acid 2 m-hydroxybenzoic acid 3 Phenol 4 Acetylsalicylic acid 5 Benzoic acid 6 Salicylic acid Column: ULTRON VX-ODS Mobile phase: CH 3 CN/H 2 O = 30/70 Temperature: 30 Detection: UV-254 nm Column: ULTRON VX-ODS Mobile phase: 10 mm (NH 4 ) 3 PO 4 (ph 2.5)/CH 3 CN = 80/20 Temperature: 30 Detection: UV-254 nm

3 ULTRON VX-Octyl ULTRON VX-Octyl columns are reversed-phase columns consisting of octyl groups (C8) bonded to a silica gel substrate. As these columns display weaker retention in comparison with ULTRON VX-ODS columns thus enabling shorter analysis times, they are particularly effective for analyses of highly hydrophobic compounds. These columns display superior stability due to the use of the high precision synthesis technology used to manufacture ULTRON VX-ODS columns. Features 1 ULTRON VX-Octyl columns provide shorter analysis times than ODS columns and can also maintain high separation capacities. 2 Volumes of organic solvents can be reduced. 3 ULTRON VX-Octyl columns have very low variations between lots and display excellent reproducibility. 4 Distribution equilibriums can be achieved quickly making these columns perfect for gradient elutions. Physical properties Particle size Pore size Specific surface area Carbon content 5 µm 12 nm 300 m 2 /g 10% Comparison of retention of alkylbenzenes using the VX-Octyl and VX-ODS Alkylbenzenes Column: ULTRON VX-Octyl 5 µm Mobile phase: CH 3 OH/H 2 O Temperature: 40 Detection: UV-254 nm

4 ULTRON VX-SIL ULTRON VX-SIL columns are normal phase columns employing high purity silica gel (>99.99%), thereby reducing sample absorption by metal oxides to the lowest possible levels. These columns are packed with spherical silica particles resulting in low analytical pressures and extended column lifetimes. Physical properties Particle size Pore size Specific surface area 5 µm 12 nm 300 m 2 /g Female hormones Fat-soluble vitamins 1 Estrone 2 Estradiol 3 Estriol 1 Vitamin A palmitate 2 Vitamin K 1 3 Vitamin K 2 4 Vitamin A acetate 5 Vitamin E Column: ULTRON VX-SIL Mobile phase: n-hexane/ethanol = 90/10 Temperature: Ambient Detection: UV-280 nm Column: ULTRON VX-SIL Mobile phase: n-hexane/dichloromethane = 90/10 Temperature: Ambient Detection: UV-254 nm

5 STR ODS-Ⅱ/ STR ODS-M STR ODS-Ⅱ columns are general purpose columns created using Shinwa s superior synthesis technology, and which display high levels of reproducibility. Features 1 The effects of metal oxides are suppressed to a minimum due to the use of highly-purified silica gel. 2 Durable due to the use of mechanically strong silica gel. 3 Easy to use and display high levels of performance due to low analytical operating pressures. 4 Excellent end capping technology provides sharp peak shapes for basic and acidic compounds, and for samples which form complexes with metals. Physical properties (STR ODS-Ⅱ) Particle size Pore size Specific surface area Carbon content 5 µm 12 nm 320 m 2 /g 17% Physical properties (STR ODS-M) Particle size Pore size Specific surface area Carbon content 5 µm 10 nm 350 m 2 /g 15% Cold medicine components Organic acids 1 Acetaminophen 2 Caffeine 3 Riboflavin 4 Phenol 5 Potassium guaiacol-4 sulfonate 1 Tartaric acid 2 Malic acid 3 Malonic acid 4 Acetic acid 5 Citric acid 6 Succinic acid 7 Malic acid degradate 8 Propionic acid Column: STR ODS-Ⅱ Mobile phase: H 2 O:CH 3 CN:50 mm Tetrabutylammonium = 15:2:1 (Adjusted to ph 6 using phosphoric acid) Temperature: 50 Detection: UV-280 nm (0.01 AUFS) Column: STR ODS-M Mobile phase: 10 mm NH 2 PO 4 (Adjusted to ph 2.3 using phosphoric acid) Temperature: 40 Detection: UV-210 nm

6 ULTRON VX-ODS Specifications ULTRON VX-ODS (Narrow bore) L1 ULTRON VX-ODS (Narrow bore) L1 ULTRON VX-ODS (Narrow bore) L1 ULTRON VX-ODS (Narrow bore) L1 ULTRON VX-ODS L1 ULTRON VX-ODS L1 ULTRON VX-ODS L1 ULTRON VX-ODS L1 ULTRON VX-ODS L1 ULTRON VX-ODS L1 ULTRON VX-ODS ULTRON VX-ODS Guard column (analytical) ULTRON VX-ODS Guard column (analytical) ULTRON VX-ODS Guard column (analytical) ULTRON VX-ODS Guard cartridges (x2) ULTRON VX-ODS Guard cartridges (x2) For mm columns For mm columns ULTRON VX-ODS PREP L1 ULTRON VX-ODS PREP L1 ULTRON VX-ODS PREP L1 ULTRON VX-ODS PREP L1 ULTRON VX-ODS PREP ULTRON VX-ODS PREP ULTRON VX-ODS PREP ULTRON VX-ODS PREP Guard column ULTRON VX-ODS PREP Guard column ULTRON VX-ODS PREP Guard column ULTRON VX-Octyl Specifications ULTRON VX-Octyl L7 ULTRON VX-Octyl L7 ULTRON VX-Octyl Guard column Preparative columns ULTRON VX-Octyl PREP L7 ULTRON VX-Octyl PREP Guard column

7 ULTRON VX-SIL Specifications ULTRON VX-SIL (Narrow bore) L3 ULTRON VX-SIL (Narrow bore) L3 ULTRON VX-SIL (Narrow bore) L3 ULTRON VX-SIL (Narrow bore) L3 ULTRON VX-SIL L3 ULTRON VX-SIL L3 ULTRON VX-SIL L3 ULTRON VX-SIL L3 ULTRON VX-SIL L3 ULTRON VX-SIL L3 ULTRON VX-SIL ULTRON VX-SIL Guard column ULTRON VX-SIL Guard column ULTRON VX-SIL Guard column ULTRON VX-SIL Guard cartridges (x2) ULTRON VX-SIL Guard cartridges (x2) For mm columns For mm columns Preparative columns ULTRON VX-SIL PREP L3 ULTRON VX-SIL PREP L3 ULTRON VX-SIL PREP L3 ULTRON VX-SIL PREP L3 ULTRON VX-SIL PREP ULTRON VX-SIL PREP ULTRON VX-SIL PREP ULTRON VX-SIL PREP Guard column ULTRON VX-SIL PREP Guard column ULTRON VX-SIL PREP Guard column

8 ULTRON STR ODS-Ⅱ/ STR ODS-M Specifications STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ L1 STR ODS-Ⅱ PEEK L1 STR ODS-Ⅱ PEEK L1 STR ODS-Ⅱ Guard column STR ODS-Ⅱ Guard column STR ODS-Ⅱ Guard column STR ODS-Ⅱ PEEK Guard column STR ODS-Ⅱ Guard cartridges (x2) STR ODS-Ⅱ Guard cartridges (x2) STR ODS-Ⅱ Guard cartridges (x2) For mm columns For mm columns For mm columns STR ODS-M L1 STR ODS-M L1 STR ODS-M L1 STR ODS-M L1 STR ODS-M L1 STR ODS-M Guard column L1 STR ODS-M Guard column L1 Preparative columns STR ODS-Ⅱ PREP L1 STR ODS-Ⅱ PREP Guard column L1 Please feel free to contact us with questions related to analyses. Column screening services are also available. Please be aware that s are subject to change without prior notification. Contact details for inquiries Shinwa Chemical Industries Ltd Kagekatsu-cho, Fushimi-ku Kyoto Japan TEL: FAX: info@shinwa-cpc.co.jp Website: SA1029A-E