LightScanner. Systems. Get There Faster. Achieve High Throughput Mutation Discovery and Genotyping.

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1 LightScanner Systems Get There Faster Achieve High Throughput Mutation Discovery and Genotyping

2 Hi-Res Melting Perfected Hi-Res Melting is a post PCR technique for homogenous mutation scanning and genotyping. FAST - Results in less than 5 minutes SIMPLE - No post-pcr processing ECONOMICAL - Costs significantly less than competing technologies The LightScanner system enables Hi-Res Melting, a straightforward and cost effective solution for today s re-sequencing and mutation discovery projects. Four Easy Steps to Results Scanning is nondestructive; samples can be recovered for additional analysis, such as sequencing, gel electrophoresis or remelting. Idaho Technology s Hi-Res Melting systems have been used all over the world and reported in over 300 peerreviewed publications. Hi-Res Melting began in 2003 with the HR-1. This unique technology has now been perfected in the currently offered LightScanner and LightScanner 32 (LS32) systems. Join in other researchers success! Jump on the LightScanner express and transport your theories into action! The Majority of HRM Publications Are Published Using the LightScanner Systems LightScanner Systems* *Publications from 2003-June Including HR-1. All Other Systems Combined

3 Two Ways To Get There Mutation Discovery (Amplicon Scanning) Mutation Scanning Data Analysis Four distinct groups can be identified by the change in the shape of the curve and by the difference compared to the wild type. Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting on the LightScanner is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples. Genotyping Genotyping with small amplicons can be successfully performed for SNPs, small deletions and insertions that are located between the 3 ends of the forward and reverse primer. No probe is required for difficult base pair neutral changes (A>T, G>C). The High Sensitivity Master Mix can overcome challenges in detecting subtle differences between these homozygous alleles. Heterozygous Double Heterozygous Homozygous G allele C Variant Single Heterozygous Wild Type Genotyping with unlabeled LunaProbes is a homogeneous end-point assay easily accomplished using the LightScanner System. The probe is included in the PCR reaction but is not consumed during amplification. Genotyping is accomplished by monitoring the melting temperature of probe-target duplexes post-pcr. G allele Heterozygous C Variant The fully matched probe-target hybrid (C variant) generates a melting peak with higher Tm (shown in blue), while the mis-matched hybrid (G allele) generates a peak at a lower Tm (shown in gray). The heterozygous samples demonstrate a profile with peaks at both Tm s, representing the presence of both alleles (shown in red). For a complete list of references using Hi-Res melting and the LightScanner system please visit scholar.google.com and type in LightScanner in the search box.

4 A Complete Product Solution The LightScanner system provides a complete solution for research laboratories wanting to perform Hi-Res Melting and mutation discovery. It s robust and reproducible results are achieved using common tools and techniques used in today s research laboratories. Integrate Seamlessly into your Current Workflow Chemistry LCGreen Plus is the optimal chemistry for mutation discovery and genotyping. The LightScanner Master Mix provides a preoptimized hot-start solution specifically designed to deliver superior Hi-Res melting results. Instrumentation The LightScanner Instrument provides the superior optical sensitivity and thermal control required for Hi-Res Melting. Compatible with industry standard 96 and 384 well plates and thermocyclers making it simple to integrate into most research labs. Support We are the experts! Idaho Technology has been developing DNA Melting applications for over 10 years. Benefit from speaking directly with the experts who pioneered the technology. Software The LightScanner Primer Design software enables users to desgin primer sets that amplify small segments specifically for mutation discovery using Hi-Res Melting. The LightScanner system features Call-IT, software that critically analyzes each sample and defines standards and variants. Idaho Technology puts innovative engineering and development to work resulting in the only system designed specifically for Hi-Res Melting. Every product at Idaho Technology is developed under our cgmp system. Selecting Idaho Technology means gaining access to those who know the field better than anyone. Designed for Hi-Res Melting. Get there faster with our expert systems, chemistry, and support. The LightScanner System - Robust Data Acquisition - Unsurpassed Temperature Control - Superior Fluorescent Chemistry - Advanced Software

5 Idaho Technology Inc. Hardware Software Support Reagents Biodefense Enviornmental Industrial Food Life Science Innovation Amplified Idaho Technology Inc. is a privately held company and home to the fastest, highest-quality machines in the world for pathogen identification and DNA analysis; including DNA amplification, real-time PCR, Hi-Res Melting, mutation detection and genotyping. For over 20 years we have been making great strides to ensure that our products remain efficient, user-friendly, and on the cutting edge of innovation. We are backed by a remarkable network of corporate and educational relationships and a growing number of committed and talented team members. 390 Wakara Way, Salt Lake City, Utah USA For additional information regarding our products and applications, please contact our Customer Service Department at Idaho Technology Inc. LSCN-BRO