UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Budget Worksheet

Transcription

1 UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Budget Worksheet BUDGET ITEM Department or College Funds Outside Agency Funds Personal Funds Undergrad. Research Funds GRAND TOTAL Materials $1,451 $1,451 Equipment Stipend: $10/hr 8.5% Total Mileage to gather Data (.36 per mile) GRAND TOTAL $1,451 $1,451 OUR Long Term Grant Application Revised November 145 Page 1 of 8

2 UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Body of Proposal Project Description The purpose of this research project is to determine if plant derived essential oils have antimicrobial disinfecting properties comparable to commercially available disinfectants. Hospitals and laboratories use chemicals such as industrial bleach for disinfecting hard surfaces. These chemicals are considered hazardous and may cause irritation to the skin, or mucous membranes, and could even damage the lungs from long-term inhalation of fumes ( Dispatch MSDS, 2011). Studies have shown that essential oils contain chemical compounds with broad antimicrobial properties (Hammer, Carson, & Riley, 1999; Mayaud, Carricajo, Zhiri, & Aubert, 2008). There have been a number of studies performed that have examined the susceptibility of bacteria to these essential oils. One such study used orange and bergamot essential oils in vapor form to effectively reduce bacterial biofilm on stainless steel surfaces after 24 hours of exposure to the vaporized oil (Laird, Armitage, & Phillips, 2012). Still other studies have tested bacterial susceptibility to essential oils using the disk diffusion method on culture plates (Elaissi et al., 2012; Galvão et al., 2012). This research proposal aims to further these studies by testing the potential disinfecting properties of lemon, geranium, clove, cinnamon, and rosemary essential oils against specific bacteria, namely; E. coli, P. aeruginosa, E. faecalis, and S. aureus, which cause four of the most common hospital-acquired bacterial infections (Hidron et al., 2008). Essential oils that show antimicrobial properties against any of the organisms will be subsequently tested for their disinfecting properties on four common hospital and lab surfaces against the same bacteria. It is expected that the essential oils included in this study will be effective at disinfecting a number of surfaces against these hospital-acquired organisms. These findings will support OUR Long Term Grant Application Revised November 145 Page 2 of 8

3 further research into the development of less toxic disinfecting products for use on hospital, laboratory or commercial surfaces. Matthew Nicholaou, DrPH, MT(ASCP), is our faculty mentor on this project. He has conducted extensive research on infectious diseases and microbiology and will serve as an advisor for the project when needed. Our research will be performed in the MLS microbiology laboratory on the campus of WSU. This project falls at the independent end of the research continuum: Dependent X_Independent All three of the students performing this research are AAS graduates of WSU in Medical Laboratory Science and are currently enrolled in the baccalaureate program. One student is a registered Medical Laboratory Technician (MLT), certified through the American Society for Clinical Pathology (ASCP) and is currently employed in a local hospital laboratory. All three students have completed over 100 credit hours and are performing this study as part of the MLS 4801 Research Projects in MLS I class, thus have the knowledge and skills needed to work in a clinical laboratory setting and to perform scientific research. Upon completion of the project, the data will be presented at the Weber State University Undergraduate Research Symposium in the spring of The results will also be submitted to the WSU undergraduate research journal, ERGO. Project Methods & Timeline Phase one of this study will consist of identifying organisms that are susceptible to one or more of, lemon, geranium, clove, cinnamon or rosemary essential oils, using the Kirby- Bauer disk diffusion method (Hudzicki, n.d.). Four ATCC organism strains of E. coli, P. aeruginosa, E. faecalis, and S. aureus, with no known antibiotic resistant genes, will be diluted to a concentration of 0.5 McFarland units as per clinical laboratory industry standards (Hudzicki, n.d.) and plated onto separate Kirby-Bauer plates. This concentration of a 0.5 OUR Long Term Grant Application Revised November 145 Page 3 of 8

4 McFarland units will be used throughout the study. Each plate will be divided into seven sections, one section for each of the five essential oils being tested, as well as a section for the water and bleach controls. 10 ul of each essential oil, and 10 ul of the water and bleach controls will be applied to paper disks, the disks will then be applied to the Kirby-Bauer plate, one to a section. The plates will then be incubated for 24 hours to see if there is a zone of inhibition (Hudzicki, n.d.). The same 0.5 McFarland solution will also be used to inoculate four surfaces: laminate, plastic, steel, and tile. After 24 hours, each surface will be swabbed with a sterile cotton swab. The swabs will then be used to inoculate an SBA plate, which will be incubated for 24 hours to check for growth. This will show that the organisms are able to survive on the surface without treatment. The organisms that are able to survive on the surfaces, and the essential oils that inhibit the bacterial growth, will be selected for phase two testing of their disinfecting properties. Using the organisms identified in phase one of testing, phase two will involve taking 1 ml of the 0.5 McFarland standard of bacteria rich solution and spreading it onto seven pieces of each surface type using a sterile cotton swab. This step will be repeated for each organism. These same surfaces will then be wiped with 1 ml of each type of essential oil, bleach, or water using a paper towel. A swab of the surface will be taken at ten minutes, one hour, eight hours, and 24 hours after application to check for bacterial growth. At 24 hours, the organism will be reintroduced to each surface, using the 0.5 McFarland standard of bacteria, and a swab will be taken 24 hours later to test for the continued antimicrobial activity of the oils. Each time a surface is swabbed, the swab will be used to inoculate an SBA plate and placed in the incubator for 24 hours to check for bacterial growth. If growth is seen, it will be graded using the plus system (1+,2+,3+,4+). OUR Long Term Grant Application Revised November 145 Page 4 of 8

5 Project Timeline Steps Description Completion Date 1 Submit grant proposal to WSU DCHP UR Committee Oct. 31, Submit grant proposal to WSU OUR Nov. 12, Perform testing Dec Data analysis Jan Write research paper Feb Create poster Feb Present at WSU Undergraduate Research Symposium Mar Prepare for other presentations Mar Budget Explanation Budget Explanation Materials Geranium essential oil 15ml) $109 Lemon essential oil 15ml) $28 Clove essential oil 15ml) $39 Cinnamon essential oil 15ml) $60 Rosemary essential oil 15ml) $39 Distilled Water (2 gal.) as a control $2 Beach Clorox industrial germicidal (1 gal.) as $12 a control Shop Towels for wiping surfaces $12 Ceramic Tile (1 case) test surface $13 Metal (material and cutting fee) test surface $50 Plastic squares (1 roll and cutting fee) test $3 surface Laminate squares (material and cutting fee) test $50 surface P. aeruginosa organism for testing $37 S. aureus - organism for testing $42 E. coli - organism for testing $47 E. faecalis organism for testing $42 Kirby bauer plates (sleeve of 10) $33 Sheep blood agar plates (7 boxes of 100 ea.) $685 Cotton swabs (sterile) (4 boxes of 200 ea.) $58 Columbia Broth (2 boxes of 20 tubes ea.) $85 Filter paper (1 box of 100) $5 Equipment Inoculation loops to inoculate plates Provided use by MLS department Incubator Provided use by MLS department Autoclave (to sterilize water) Provided use by MLS department Grand Total $1,451 OUR Long Term Grant Application Revised November 145 Page 5 of 8

6 References Dispatch by Clorox bleach germicidal cleaner material safety data sheet, (2011) Retrieved October 17, 2012 from rmsds pdf Elaissi, A., Rouis, Z., Abid, N. B. S., Mabrouk, S., Ben Salem, Y., Bel Haj Salah, K., Aouni, M., et al. (2012). Chemical Composition of 8 Eucalyptus species Essential Oils and the Evaluation of Their Antibacterial, Antifungal and Antiviral activities. BMC complementary and alternative medicine, 12(1), 81. doi: / Galvão, L. C. D. C., Furletti, V. F., Bersan, S. M. F., da Cunha, M. G., Ruiz, A. L. T. G., de Carvalho, J. E., Sartoratto, A., et al. (2012). Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects. Evidence-based complementary and alternative medicine : ecam, 2012, doi: /2012/ Hammer, K. a, Carson, C. F., & Riley, T. V. (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of applied microbiology, 86(6), Retrieved from Hidron, A. I., Edwards, J. R., Patel, J., Horan, T. C., Sievert, D. M., Pollock, D. a, & Fridkin, S. K. (2008). NHSN annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the National Healthcare Safety Network at the Centers for Disease Control and Prevention, Infection control and hospital epidemiology : the official journal of the Society of Hospital Epidemiologists of America, 29(11), doi: / Hudzicki, J. (n.d.). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. Retrieved November 8, 2012, from Laird, K., Armitage, D., & Phillips, C. (2012). Reduction of surface contamination and biofilms of Enterococcus sp. and Staphylococcus aureus using a citrus-based vapour. The Journal of hospital infection, 80(1), doi: /j.jhin Mayaud, L., Carricajo, a, Zhiri, a, & Aubert, G. (2008). Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics. Letters in applied microbiology, 47(3), doi: /j x x OUR Long Term Grant Application Revised November 145 Page 6 of 8

7 UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION Additional Questions 1. What funding have you received from OUR in the past? Where has your previous project been disseminated? There have been no previous projects completed by this group of students. 2. Is this project part of a required course? If so, please indicate the support (monetary and in-kind) provided for this project by the academic department. This project is part of the required MLS 4801 class. There is no monetary support being provided by the MLS department. The MLS department will be providing space and equipment (incubator) for use during this project. 3. What additional sources of funding have been solicited? Is your department willing/able to fund any equipment they will be retaining? There have been no additional sources of revenue solicited for this project. The MLS department is not willing/able to fund any of the equipment being purchased for this project. 4. Where do you plan to disseminate the results of this project? We plan on submitting the results of this study to the WSU Undergraduate Research Symposium, and to the WSU undergraduate research journal, ERGO. 5. If you are requesting a stipend, please list all significant time commitments (5+ hours per week) that you expect to maintain over the duration of your project including, for example, class and work schedules. No stipend is being requested. OUR Long Term Grant Application Revised November 145 Page 7 of 8

8 UNDERGRADUATE RESEARCH LONG TERM GRANT APPLICATION FACULTY MENTOR RECOMMENDATION FORM Student Name (last, first): Sorenson, Sherry Project Title: _ Evaluation of Essential Oils for Surface Disinfecting Mentor Directions: After carefully reviewing the proposal and assessing both the viability of this project and the qualifications of the student requesting funding, answer the questions found below. Please expand the sections as necessary (do not attach separate letter). If the project involves the use of human subjects or protected animals, be sure the student secures IRB or ACUC approval. If the project receives funding, it is your responsibility to work closely with the student, monitor the ongoing progress of the project and budget, and evaluate the project s results. Failure to do so will jeopardize funding for this project and any future projects. 1. How long and in what capacity have you known this student? 2. Briefly describe the proposed project. Is this part of a larger research project? Is this part of a course? If so, how is the project apart from the nature and scope of activities normally taken for the course (Please attach a copy of your course syllabus)? 3. Give an assessment of the project s significance to the student s discipline and of the project s educational and/or professional benefit to the student. 4. Comment on the qualifications of the student to successfully complete this project, both in terms of the project s scope and its time frame. 5. Comment on the justification and appropriateness of the project budget, including the necessity of a stipend (if requesting one). 6. Describe your role in the project. 7. Include anything else that you think will be helpful to the committee in evaluating this application. This project DOES DOES NOT require review by the WSU Institutional Review Board for Human Subjects or the WSU Animal Care and Use Committee. Project Mentor Signature Date Campus Mail Code Phone Extension OUR Long Term Grant Application Revised November 145 Page 8 of 8