Cellab GmbH - Introduction. June 2014

Transcription

1 Cellab GmbH - Introduction June 2014

2 Content overview About Cellab GmbH Concept Introduction Applications Technology Summary 2

3 3 About Cellab GmbH

4 Corporate structure Founded 1993 Plant engineering and manufacturing hollow fiber modules Founded 2010 OEM dialyzer manufacturing Founded 2014 Cellab Bioreactor System 4

5 Location 5

6 Cellab Bioreactor System Introduction to the concept 6

7 Current disposable cell culturing products fall short Multi-well plate Culture bag The challenge: Increased demand for precision cell culturing for e.g. regenerative medicine becomes more and more important Roller bottle T-flask Petri dish Wasteful and poorly representative for in-vivo conditions 7

8 The Answer the Cellab Bioreactor System Waste and medium bags Hollow fiber bioreactor Oxygenator Reusable docking station with controller Cellab Bioreactor System: Easy-to-use and closed disposable bioreactor system Single-packaged and sterilized disposable set High cell densities with minimal intervention and reduced media volumes Parallel cultivation of different cell lines possible Reduced media and purification costs Reduced handling time Compact and reliable delivering precise and repeatable results 8

9 Cellab Bioreactor System Insert Supply System 9 Disposable Set Docking Station

10 Cellab Bioreactor System Applications 10

11 Applications Protein production e.g. MABs Biomass production e.g. MSCs Artificial skin and other tissues 3D tissues e.g. cartilage and bone Insert Supply System (manual process) Disposable Set with Media Supply Plate + inserts (automatic process) Disposable Set with hollow fiberbioreactors (automatic process) Disposable Set with scaffold holders (automatic process) 11

12 Production of monoclonal antibodies (MABs) Efficient MAB production with high cell concentrations Achieves times higher cell densities Reduced production costs up to 60% per mg antibodies Parallel cultivation of up to 24 different hybridoma cell lines using membranebased inserts Parallel cultivation of up to 3 different hybridoma cell lines using hollow fiber bioreactors Production rates from 10 to 100 mg MABs depending on the bioreactor Manual or automated cultivation process 12

13 Cultivation of mesenchymal stem cells (MSCs) Cultivation of stem cells over several weeks without loosing multipotency Differentation to different cell lines and tissue types 21 days without non specific or spontaneous differentation Reduces significant handling time and use of consumable Parallel cultivation of up to 96 samples Cultivation with different bioreactor configurations (hollow fiber bioreactors, flat membrane inserts and scaffold holders) 13

14 Cultivation of human artificial skin models Cultivation of skin models for in-vitro testing Customized cultivation with membrane based inserts in different properties (material, poresizes) Reduced culturing costs by up to 40 % Repeatable tissue quality through standardized production processes Parallel cultivation of up to 192 human artificial skin samples 14

15 Cultivation of cartilage or bone Cultivation of cartilage or bone tissue in a 3D-matrix provided by a scaffold Customized cultivation by using customer specific scaffolds with individual properties (material, dimension) Standardized production of high cell concentration with minimal intervention Visual inspection without interruption of production process 15

16 Cellab Bioreactor System Technology 16

17 Hollow fiber bioreactor Areas of high cell density between fibers Hollow fiber lumen Separation of cells and medium by semi-permeable membrane Exchange of nutrients, oxygen and waste products via diffusion process Cells can be grown either inside or outside on the hollow fiber 17 Consistent and evenly distribution of medium and oxygen as well as the removal of metabolites increases Bioreactor contains repeatability hundreds or thousands of hollow fibers

18 Insert Supply System Insert Supply System with Media Supply Plate and 6 Diaserts Separation of cells and medium by semi-permeable membrane Exchange of nutrients, oxygen and waste products via diffusion process Cell seeding and culturing on the flat membrane surface area Individual cultures in each insert Up to 96 standard membrane based inserts (e.g. NUNC) can be fitted in a single Media Supply Plate Single Diasert with a 12,5 kda dialysis membrane Results in higher cell densities and higher production rates 18

19 Scaffold holder bioreactor Scaffold Disposable System is available for porous 3D-scaffolds of various shapes and materials Scaffolds are seeded by passing the cell suspension back and forth through the scaffold Excellent in-vitro testsystem for various scaffolds 19

20 MySolution according to your configuration needs MySolution: Fits into standard incubators Footprint: 400 x 340 x 95 mm [LxWxH] Provides a standardized platform with closed tubing system Simple integration of a customer bioreactor with standard luer lock connections Different sized media bags Disposable Set delivered single packed and sterilized 20

21 Docking Station with Cellab Control Center Scope of delivery: Docking Station with media pump, gas pump, temperature sensors Cellab Control Center Power supply cable (3m) USB-connection cable (3m) External temperature sensor Gas injection filter 21 Software features: Manual or automatic control of medium flow rate Oxygen supply adjustment in single steps Writing an individual cultivation protocol Temperature measuring and recording

22 Dimensions Cellab Bioreactor System Docking Station with Disposable Set: Fits into standard incubators Footprint: 400 x 340 x 95 mm [LxWxH] Insert Supply System: Stackable with any standard multiwell plates Footprint: 130 x 85 x 35 mm [LxWxH] 22

23 Cellab Bioreactor System Summary Superior Quality Cultures: High density cell cultures Higher production rates More representative 3D cultures Advantages in product handling: Closed system to reduce risk of contamination Less cell transference and reduced purification Ease of performing multiple, parallel cell lines Reduced media costs Reduced handling time Lower cost per culture Design simplicity Enhanced flexibility Efficient use of existing infrastructure 23

24 31 Hölzel Diagnostika Handels GmbH Hohenzollernring 38 D Köln Tel.: +49-(0) Fax: +49-(0)