Favorgen EtBr Destroyer

Transcription

1 Favorgen EtBr Destroyer

2 Favorgen EtBr Destroyer Description Favorgen EtBr Destroyer is a specifically designed reagent effectively degrade and destroy the Ethidium Bromide and result in non-fluorescence and nonmutagenic remain. And also it has been demonstrated that its effectiveness of destructing the SYBR DYE. This product is provided in two different formats for the treatment of solid or liquid Ethidium Bromide contaminant. The ready pack Favorgen EtBr Destroyer Bag is for the treatment of liquid Ethidium Bromide Contaminant. The Favorgen EtBr Destroyer Sprayer is for the treatment of solid Ethidium Bromide contaminant.

3 Features Effective: EtBr destroyer can destroy EtBr and other SYBR Dye. This effect can be monitored and confirmed by UV light exposure. Once destroyed, the fluorescence will disappear. The blocking of mutagenic effect of EtBr Destroyer has been demonstrated by Ames Test. Specific: Two specific designs are for different purposes: EtBr solid contaminant treatment and EtBr liquid contaminant treatment, i.e. the sprayer format and the ready pack format. Economical: 1. EtBr destroyer sprayer content can be refilled after use up. The cost is very economic. 2. After EtBr Destroyer treatment, theethidium Bromide-Containing Buffer can be save for further use after being filtrated. The reduction of waste volume can save the cost of waste treatment. Safe: The blocking of mutagenic effect of EtBr Destroyer has been demonstrated by Ames Test.

4 Applications I EtBr Destroyer Application for EtBr Contaminant

5 1. EtBr Destroyer Application for Solid EtBr Contaminant Non treatment of solid EtBr Contaminant Treatment of solid EtBr Contaminant

6 2. EtBr Destroyer Application for Liquid EtBr Contaminant 1. Before adding EtBr Destroyer 2. Add EtBr Destroyer 3. Completion Storage Store this product at room temperature. Valid for 6-12 months after sealing off.

7 3. Favorgen EtBr Destroyer Bag Test 100 bp DNA marker runs on a 1.2 % agarose gel The strain of Ames test S. Ryphimurium (TA ) Dip gel into 10μg/ml EtBr solution for 10 minutes Genotype confirmation of The strain Destain for 30 mins A. Non-stain Destain for 60 mins A. Non-stain B. Destain by ddwater B. Destain by ddwater C. Destain by 2L ddwater/destroyer bag C. Destain by 2L ddwater/destroyer bag D. Destain by 3L ddwater/destroyer bag D. Destain by 3L ddwater/destroyer bag Ames test Take pictures

8 The result of Favorgen EtBr Destroyer Bag Test 100 bp DNA marker runs on a 1.2 % agarose gel Dip gel into 10μg/ml EtBr solution for 10 minutes Take pictures in UV box A. Non-stain Destain for 30 mins Take pictures in UV box C. Destain by 2L ddwater/destroyer Bag B. Destain by ddwater D. Destain by 3L ddwater/destroyer bag A. Non-stain Destain for another 30 mins Take pictures in UV box C. Destain by 2L ddwater/destroyer Bag B. Destain by ddwater D. Destain by 3L ddwater/destroyer bag

9 Applications II EtBr Destroyer Application for SYBR DYE

10 EtBr Destroyer (Bag) application for SYBR Green I

11 Test of Favorgen EtBr Destroyer (Bag and Sprayer) about SYBR Green I 100 bp DNA marker runs on a 1.2 % agarose gel Gel is stained by SYBR Green I for 30min Dip gel into 3 L H 2 O for 30 minutes Dip gel into 3 L H 2 O + destroyer bag for 30 minutes Spray gel 3 times and wait for 30 minutes wait for 30 minutes Take pictures Take pictures Dip gel into 3 L H 2 O for 30 minutes Dip gel into 3 L H 2 O + destroyer bag for 30 minutes Spray gel 3 times and wait for 30 minutes wait for 30 minutes Take pictures Take pictures

12 Gel is stained by SYBR Green I for 30min Destain by 3L H 2 O for 30 min Destain by 3L H 2 O + Destroyer Bag For 30 mins Take pictures Destain by 3L H 2 O for 30 min again Destain by 3L H 2 O + Destroyer Bag for another 30 mins Take pictures

13 EtBr Destroyer (Sprayer) application for SYBR Green I

14 Gel is stained by SYBR Green I for 30min Spray gel 3 times and wait for 30 minutes wait for 30 minutes Take pictures Spray gel additional 3 times and wait for more 30 minutes wait for another 30 minutes Take pictures

15 EtBr Destroyer (Bag) application for SYBR Gold

16 Gel is stained by SYBR Gold for 30min Destain by 3L H 2 O for 30 min Destain by 3L H 2 O + Destroyer Bag For 30 mins Take pictures

17 EtBr Destroyer (Sprayer) application for SYBR Gold

18 Gel is stained by SYBR Gold for 30min wait for 30 minutes Spray gel 3 times and wait for 30 minutes wait for 30 minutes Spray gel 3 times and wait for 30 minutes Take pictures Take pictures

19 EtBr Destroyer (Bag) application for SYPRO ORANGE

20 The gel was stained with SYPRO Orange for 1 hour Destained with 3 L ddh 2 O Destained with 3 L ddh 2 O + destroyer bag Destained for 30 min Destained for 30 min again

21 EtBr Destroyer (Sprayer) application for SYPRO ORANGE

22 The gel was stained with SYPRO Orange for 1 hour Destained with spaying EtBr destroyer sprayer 3 times (about 0.9 ml) and then placed for 30 min Placed for 30 min Spraying EtBr destroyer sprayer 3 times (about 0.9 ml) again and placed for 30 min Placed for 30 min again

23 Comparison with other products Product Manufacturer Type Usage Procedure Capacity Working Time Rate (L/hr) Application Destroy Target Pack Favorgen EtBr Destroyer Favorgen Bag / Sprayer 1. Bag dissolve on the contaminated buffer 2. Spray on the contaminated Target mg/per bag ml/bottle 144hours for 30mg/3L 1. Gel 2. Ethidium Bromidecontaining buffer Ethidium Bromide SYBR Green SYBR Gold SYPRO Orange 1. Pack of 20bags 2 Pack of 2 bottles (200ml/per bottle) Ethidium Bromide Adsorber Merck Disposable gravity column Flow-through adsorption 300 mg/per column 150 hours for 300mg/30L Ethidium Bromidecontaining buffer Ethidium Bromide Packs of 4 Columns Extractor EtBr Waste Reduction System Schleicher & Schuell Disposable vacuum column Flow-through adsorption 0.5L 60L/hr Ethidium Bromidecontaining buffer Ethidium Bromide Pack of 6 Column Bind-ET System Elchrom Scientific Gravity column Flow-through adsorption 4L 0.5L/hur Ethidium Bromidecontaining buffer Ethidium Bromide Pack of 1 cartridge Fluoraway Ethidium Bromide Remover Laboratory Impex Research Ltd. Cartridge vacuum column Flow-through adsorption 1L 6L/hur Ethidium Bromide Solution Ethidium Bromide Pack of 2 catridges

24 Order Information Catalogue No. Contents Packing FAEBD 001 Favorgen EtBr Destroyer Bag 1 bag can be use up to 3L EtBr solution. 20 bag/ box FAEBD Favorgen EtBr Destroyer Bag 1 bag can be use up to 3L EtBr solution. 10 bag/ box FAEBD 002 Favorgen EtBr Destroyer Sprayer Each spray contains 0.4ml 1 sprayer can be used up to 600 times 2 sprayer (200ml) /pkg 1 Sprayer Head FAEBD Sprayer supplement 1 bottle 200ml Packed 2 bottle in a retail box 2 sprayer (200ml x 2) FAEBD Sprayer supplement 1 bottle/ 1L Packed 1 bottle 1 sprayer (1L x 1) FAEBD 003 Favorgen EtBr Destroyer Bag and Sprayer 5 bags 1 sprayer (200ml) /pkg 1 Sprayer Head

25 Thanks for your attention.