See. Beyond HALO. and Sigma-Aldrich Biotechnology. Advanced Materials Technology. Ascentis. Express

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1 See Beyond Advanced Materials Technology HALO and Sigma-Aldrich Biotechnology Ascentis Express

2 See the Performance Difference Decreased secondary interactions with a more inert base material Sharper peaks for greater resolution Increased sensitivity (S/N ratio) with taller peaks Ranitidine Kinetex min A s =. 6 APP ID 967 Insulin Impurity Profiling Kinetex 8. 4 R s (peaks & 4) = min 4 APP ID 9677 Advanced Materials HALO Technology min A s =.9 6 APP ID Advanced Materials HALO Technology 67 4 R s (peaks & 4) = min 4 APP ID 9678 Sigma-Aldrich Biotechnology Ascentis Express min A s =.6 6 APP ID Sigma-Aldrich Biotechnology Ascentis Express.7 4 R s (peaks & 4) = min 4 APP ID 9679 Conditions for all columns: Columns: Kinetex.6 µm C8 Å HALO.7 µm C8 9 Å Ascentis Express.7 µm C8 9 Å Dimensions: x 4.6 mm Mobile Phase: A: 4 mm Potassium phosphate, ph 7. / Acetonitrile (98:) B: 4 mm Potassium phosphate, ph 7. / Acetonitrile (78:) Gradient: Time (min) % B low Rate:. ml/min Temperature: C Detection: nm Sample:. Ranitidine amino alcohol hemifumarate. Ranitidine diamine hemifumarate (Related compound A). Ranitidine S-oxide (Related compound C) 4. Ranitidine N-oxide. Ranitidine complex nitroacetamide 6. Ranitidine HCl, Inc. All rights reserved. USA Tel: -- Puerto Rico Tel: Conditions for all columns: Columns: Kinetex.6 µm XB-C8 Å HALO.7 µm C8 9 Å Ascentis Express.7 µm C8 9 Å Dimensions: x 4.6 mm Mobile Phase: A:. % Trifluoroacetic acid (TA) in Water B:. % Trifluoroacetic acid (TA) in Acetonitrile Gradient: Time (min) % B 6 low Rate:. ml/min Temperature: 4 C Detection: 4 nm Sample:. Insulin. Sample Impurity Tel: ax: info@phenomenex.com. Sample Impurity 4. Sample Impurity HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications.

3 See the Particle Difference Advanced particle morphology and uniformity Kinetex Advanced Materials Technology HALO vs. Kinetex Sub- µm Particle HALO Ascentis Express.6 µm Total Particle Size.7 µm.7 µm. µm Porous Shell. µm. µm.9 µm Solid Core.7 µm.7 µm /6 bar* Pressure Limit 6 bar 6 bar Å Pore Size 9 Å 9 Å. ** ph Range 9 9 m /g Effective Surface Area ~ m /g ~ m /g Sub- µm Particle.7 µm Total Particle Size - -. µm Porous Shell - -. µm Solid Core - - bar Pressure Limit - - Å Pore Size - -. ** ph Range - - m /g Effective Surface Area - - *. mm ID Kinetex columns are pressure stable up to bar. ** Columns are ph stable from. - under isocratic conditions. Columns are ph stable from - 8 under gradient conditions. Advanced Materials Technology PRODUCT DOES NOT EXIST Sigma-Aldrich Biotechnology PRODUCT DOES NOT EXIST Nearly monodispersed particle size distribution Excellent batch-to-batch reproducibility Particle Count Particle Diameter (µm) Kinetex DP 9/ =. Advanced Materials Technology Halo DP 9/ =. Kinetex.6 µm C8 Core-Shell Traditional ully Porous C8.7 µm Halo.7 µm C8 4 6 Batch 68 R s (,). Batch 7 R s (,). Batch 7 R s (,).6 4 Kinetex.6 µm C8 Kinetex.6 µm C8 Kinetex.6 µm C8 Batch 68 Batch 7 Batch 7 4 min APP ID HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications. USA Tel: -- Puerto Rico Tel: Tel: ax: info@phenomenex.com

4 See Amazing Resolution with Complementary Selectivities Even more than efficiency, selectivity is the most important parameter for obtaining increased resolution. Tivadar arkas, Ph.D., Senior Managing Scientist Kinetex C8 This phase offers the hydrophobic retention and methylene selectivity chromatographers expect from a C8 column. NEW! Kinetex XB-C8 This new phase provides superior peak shape and separation of basic compounds. C8 ph Range:. * USP Classification: L Particle Sizes:.6 µm &.7 µm Effective Carbon Load: % Si XB- C8 ph Range:. * USP Classification: L Particle Sizes:.6 µm &.7 µm Effective Carbon Load: % Kinetex PP This pentafluorophenyl phase incorporates fluorine atoms on the periphery of the phenyl ring to provide unique aromatic and polar selectivity. NEW! Kinetex C8 This new phase brings the benefits of core-shell technology to USP L7 and other C8 column methods. PP ph Range:. 8 USP Classification: L4 Particle Sizes:.6 µm &.7 µm Effective Carbon Load: 9 % C8 ph Range:. * USP Classification: L7 Particle Sizes:.6 µm &.7 µm Effective Carbon Load: 8 % Kinetex HILIC H ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN ACN H ACN ACN ACN ACN H H H H HO HO O H OH H H O H HO H Si H OH Si Si OH This unbonded silica phase is used under hydrophilic interaction chromatography conditions to provide the ideal selectivity for the retention and separation of polar compounds. HILIC ph Range:. 7. USP Classification: L Particle Sizes:.6 µm &.7 µm 4 * ph range under isocratic conditions. ph range is 8 under gradient conditions. USA Tel: -- Puerto Rico Tel: Tel: ax: info@phenomenex.com

5 See What Other Scientists are Saying Never had such a low reduced HETP value been achieved in column manufacturing technology.. Gritti et al. / J. Chromatogr. A 7 () 89-6 Increased sensitivity, S/N ratio Narrower peak widths Less tailing R&D Award Recipient rom the Journal of Chromatography A (A) 6 4 Abs [] nm CH CN/H /TA, /69/., v/v/v 9 K,. ml/min Kinetex Advanced Materials Technology HALO Recent publications in the Journal of Chromatography A compare Kinetex core-shell technology columns to other popular UHPLC technologies. Articles. Performance of columns packed with the new shell particles, Kinetex-C8 Journal of Chromatography A, Volume 7, Issue, March, Pages By abrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon.. Performance of columns packed with the new shell Kinetex-C8 particles in gradient elution chromatography Journal of Chromatography A, Volume 7, Issue, March, Pages By abrice Gritti and Georges Guiochon. (B) 9 6 Abs [] nm 4 min Advanced Materials Technology HALO CH CN/H /TA, /69/., v/v/v 9 K,.9 ml/min Kinetex. Comparative study of new shell type (Kinetex), sub- μm fully porous and monolith stationary phases, focusing on mass transfer resistance Journal of Chromatography A, Volume 7, 4 June, Pages By Erzsébet Oláh, Szabolcs ekete, Jenö ekete, and Katalin Ganzler. Access these and additional journal articles by visiting 4 min ig. 7. Comparison between the peak shapes of insulin (A) and lysozyme (B) recorded on the Kinetex and Halo columns. Reprinted from Journal of Chromatography A, Volume 7, Issue, with permission from Elsevier. Performance of columns packed with the new shell particles, Kinetex- C8, page 98, copyright. By abrice Gritti, Irene Leonardis, David Shock, Paul Stevenson, Andrew Shalliker, and Georges Guiochon. HALO is a registered trademark of Advanced Materials Technology, Inc. Ascentis is a registered trademark of Sigma-Aldrich Biotechnology LP. All columns used for comparison were new and manufactured by Advanced Materials Technology, Inc. and Sigma-Aldrich Biotechnology LP, respectively. is in no way affiliated with the above companies. Dimensions and chromatographic conditions are the same for all columns compared unless otherwise noted. Comparative separations may not be representative of all applications. USA Tel: -- Puerto Rico Tel: Tel: ax: info@phenomenex.com

6 Ordering Information KrudKatcher Kinetex.6 μm Analytical Columns (mm) Ultra In-Line ilter* x 4.6 x x 4.6 x 4.6 x mm ID $ 79 $ 69 $ 679 $ 699 $ 749 $ 89/pk XB-C8 B-4496-E C-4496-E D-4496-E E A-8497 C8 A-446-E B-446-E C-446-E D-446-E -446-E A-8497 C8 B-4497-E C-4497-E D-4497-E E A-8497 PP A-4477-E B-4477-E C-4477-E D-4477-E E A-8497 HILIC B-446-E C-446-E D-446-E -446-E A-8497 KrudKatcher Kinetex.6 μm MidBore Columns (mm) Ultra In-Line ilter* x. x. 7 x. x. x. 4.6 mm ID $ 79 $ 69 $ 679 $ 699 $ 749 $ 89/pk XB-C8 B-4496-Y D-4496-Y A-8497 C8 A-446-Y B-446-Y C-446-Y D-446-Y -446-Y A-8497 C8 B-4497-Y D-4497-Y A-8497 PP A-4477-Y B-4477-Y C-4477-Y D-4477-Y Y A-8497 HILIC -446-Y A-8497 KrudKatcher Kinetex.6 μm Minibore Columns (mm) Ultra In-Line ilter* x. x. x. x. 4.6 mm ID $ 79 $ 69 $ 699 $ 749 $ 89/pk XB-C8 A-4496-AN B-4496-AN D-4496-AN AN A-8497 C8 A-446-AN B-446-AN D-446-AN -446-AN A-8497 C8 A-4497-AN B-4497-AN D-4497-AN AN A-8497 PP A-4477-AN B-4477-AN D-4477-AN AN A-8497 HILIC B-446-AN D-446-AN -446-AN A-8497 KrudKatcher Kinetex.7 μm Minibore Columns (mm) Ultra In-Line ilter* x. x. x. 4.6 mm ID $ 799 $ 89 $ 869 $ 89/pk XB-C8 B-4498-AN D-4498-AN A-8497 C8 B-447-AN D-447-AN -447-AN A-8497 C8 B-4499-AN D-4499-AN A-8497 PP B-4476-AN D-4476-AN AN A-8497 HILIC B-4474-AN A-8497 KrudKatcher Ultra Extends Kinetex and UHPLC column lifetimes Easy to use Pressure rated to, psi (,7 bar) Extremely low dead volume minimizes sample peak dispersion The KrudKatcher Ultra filter body houses an integrated. μm 6 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system backpressure or dead volume (<. μl). *KrudKatcher Ultra requires /6 in. wrench. Wrench not provided. UHPLC / HPLC Sure-Lok Pressure PEEK Male Nut ittings Part No. Description Unit Price AQ-8 Sure-Lok Pressure PEEK -Pc Nut -, /pk $ 7 for / 6 in. Tubing,, psi (87 bar) AQ-8 Sure-Lok itting Tightening Tool, Aluminum ea 6 AQ-8 AQ-8 6 USA Tel: -- Puerto Rico Tel: Tel: ax: info@phenomenex.com

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