Cancer inflammation research applications and products

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1 Cancer inflammation research applications and products Flow cytometry Immunoassays Cell imaging Instrumentation Invitrogen Attune NxT Flow Cytometer Antibodies RNA flow Conjugated antibodies for flow cytometry Invitrogen PrimeFlow assay Stimulation Activating and neutralizing antibod

2 Better understanding of the complex systems of inflammation and the role they play in cancer is important to oncology research, making improvements in cancer diagnosis and the development of targeted therapies. With expertise that spans cellular and protein analysis, we offer innovative products that can accelerate inflammation and cancer research. Together, our affordable, highquality research platforms and reagents are designed to help you reach your cancer inflammation research goals. Flow cytometry Cellular phenotyping with our flow cytometry solutions Invitrogen products can help you maximize the power of multicolor flow cytometry for your experiments with the right combination of next-generation instrumentation and high-performance reagents that are validated, optimized, and designed to create bright, beautiful, and consistent results. We make it easier for you to perform great flow cytometry. Instrumentation Invitrogen Attune NxT Flow Cytometer Antibodies RNA flow Stimulation Conjugated antibodies for flow cytometry Invitrogen PrimeFlow assay Activating and neutralizing antibodies Cell health reagents Everyday essentials Apoptosis, proliferation, viability Beads, standards, controls and fixation

3 Immunoassays Cytokine profiling with our immunoassays Invitrogen ELISAs and Luminex multiplex immunoassays can be used for quantitative assessment of soluble proteins such as cytokines, chemokines, growth factors, and other immunological markers. ELISAs We offer both coat-it-yourself (uncoated) and ready-to-use (precoated) ELISA formats, with the necessary reagents included to run your assay. Multiplex assays Our Invitrogen ProcartaPlex assays are available as catalog multiplex panels, simplex singleplex sets, and mix and match custom panels for broad flexibility. Broad menu over 700 cytokine, chemokine, and growth factor targets Large screening range quantitatively measure up to 80 different targets simultaneously Shorter protocol time to results in 2.5 to 4 hours (varies by kit) Reduced sample 50 µl or less per well, which is less than the leading competitor ELISA kits Large panel menu ranging from 3-plex to 65-plex, available in human, mouse, rat, and other target species Highly flexible expand or change your biomarker profile as you need with our premixed custom panel option Minimal sample volumes uses as Cell health Featured product reagents Everyday essentials Beads, ProcartaPlex Human Immune Monitoring Panel (65 plex) Highest-plexed screening tool on the market for measuring cytokine proteins in Apoptosis, proliferation, viability standards,human controls immune targets. and fixation per well to measure 65 different Analytes include: APRIL BAFF BLC CD30 CD40L ENA-78 Eotaxin Eotaxin-2 Eotaxin-3 FGF-2 Fractalkine G-CSF GM-CSF GRO alpha HGF IFN-a IFN-g IL-10 IL-12p70 IL-13 IL-15 IL-16 IL-17A IL-18 IL-1a IL-1b IL-2 IL-20 IL-21 IL-22 IL-23 IL-27 IL-2R IL-3 IL-31 Find out more about our complete immunoassay portfolio at thermofisher.com/immunoassays IL-4 IL-5 IL-6 IL-7 IL-8 IL-9 IP-10 I-TAC LIF MCP-1 MCP-2 MCP-3 M-CSF MDC MIF MIG MIP1a MIP-1b MIP-3a

4 Antibodies for flow cytometry We offer over 10,000 conjugated antibodies specifically developed, validated, and manufactured for flow cytometry to investigate CD markers, oncology markers, stem cells, cell signaling, cytokines and chemokines, as well as a multitude of key cell types, such as: B cells Dendritic cells Embryonic stem cells Hematopoietic stem cells Macrophage and monocyte cells Mesenchymal stem cells NK and NKT cells Neuronal stem cells T cells T regulatory cells Th1 cells Th2 cells Th17 cells Tfh cells Find out more at thermofisher.com/antibodies Attune NxT Flow Cytometer Speed isn t just about going fast, it s about discovery. Get high levels of data fidelity 10x faster than ever before. Enable more investigate more sample types, including tumor samples, without losing your precious samples Expand your capabilities grow as your needs expand, with field upgradability of up to 4 lasers and 16 detection channels Simplified sample prep no-wash, no-lyse sample prep options with clog-resistant fluidics Option for walk-away automation plate autosampler with clog-resistant design Run samples faster and achieve greater resolution reduced loss due to clogging The Attune NxT Flow Cytometer combines precision with performance in a true benchtop flow cytometer. Do more. Find more. Explore more. Find out more at thermofisher.com/flowcytometry

5 Laser configurations and antibody options for the Attune NxT Flow Cytometer Excitation laser Channel Antibody labels Blue yellow/ violet violet/ green violet violet/ yellow violet/ green violet red green Super Bright 436 VL1 (440/50) Violet (405 nm) Alexa Fluor 405 efluor 450 Pacific Blue VL2 (512/25) VL3 (603/48) VL4 BL1 (525/50) efluor 506 Pacific Green Super Bright 600 Pacific Orange Super Bright 645 FITC Alexa Fluor 488 PE BL2 (590/40) PE-Alexa Fluor 610 PE-Texas Red Blue (488 nm) PE-Cy 5 BL3 (695/40) PE-Cy 5.5 PerCP-Cy5.5 PerCP-eFluor 710 BL4 (780/60) PE-Cy 7 Alexa Fluor 532 GL1 (575/36) Alexa Fluor 555 PE Green (532 nm) PE-eFluor 568 GL2 (620/15) Alexa Fluor 594 PE-Texas Red GL3 (695/40) PE-Cy5.5 GL4 (780/60) PE-Cy7 YL1 (585/16) PE PE-eFluor 610 YL2 (620/15) Yellow (561 nm) Alexa Fluor 568 PE-Texas Red YL3 (695/40) PE-Cy5 PE-Cy5.5 YL4 (780/60) PE-Cy7 APC RL1 (670/14) efluor 660 Alexa Fluor 647 Alexa Fluor 680 Red (637 nm) RL2 (720/30) Alexa Fluor 700 APC-Alexa Fluor 700 Alexa Fluor 750 RL3 (780/60) APC-Alexa Fluor 750 APC-eFluor 780 APC-Cy7

6 Cell imaging Cellular and tissue staining for protein localization The Invitrogen EVOS FL Auto 2 Imaging System with optional Onstage Incubator is designed to eliminate the complexities of long-term live-cell imaging, enabling researchers to focus on data generation instead of instrument operation and maintenance. It allows for time-lapse cell imaging for long-term monitoring of cell cultures. This flexible, high-performance, and affordable solution for live-cell imaging offers: Control easily control environmental and image acquisition parameters High detectability create time-lapse images of every well of a 96-well plate Savings low cost of ownership and operation Find out more at thermofisher.com/evos Count on Invitrogen antibodies >74,000 highly validated primary and secondary antibodies, with reactivities for >60 species, and growing Invitrogen antibodies support 24 9 major application areas Flow cytometry ELISA Immunofluorescence Immunoprecipitation Immunohistochemistry Western blot and more different colors of Invitrogen Alexa Fluor dyes for fluorescently labeled secondary antibodies Our primary antibodies cover over 85% Find out more at thermofisher.com/antibodies Find out more at thermofisher.com For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Luminex is a registered trademark of Luminex Corporation. Cy is a registered trademark of GE Healthcare. COL of the proteome