everything your link to. KITS & REAGENTS BIOTINYLATION KITS Biotinylate & quantify labeling with 1 reagent

Transcription

1 everything your link to. KITS & REAGETS BITIYLATI KITS Biotinylate & quantify labeling with 1 reagent MAGETIC BEADS ighest binding capacity streptavidin, 4-FB, & amino versions CRSSLIKIG KITS Efficient & easy-to-use antibody labeling o chromatography required

2 Crosslinking and Antibody anolink and MagnaLink Magnetic Beads ChromaLink Biotin Labeling Kit LIKER ACTIVATED, IGEST BIDIG CAPACITY TE MARKET anolink and MagnaLink beads reduce the overall bead mass required to immobilize a biotinylated sample. This provides the benefits of both lower backgrounds of nonspecific binding and lower net costs. ChromaLink Biotin links traceable biotins on any amine-containing biomolecule or surface. The kit allows for the simple and direct UV quantification of total incorporated biotin on antibodies and other proteins. Learn more on our website: Complete Kit: All necessary reagents included Built-in Quantitative Signal: Incorporated biotin quantified by a simple absorbance 28 and 354 reading Versatile: Labels 25 µg to 1 mg of antibody Safe & Economical: Water-soluble biotin reagent, mild on antibodies; no organics needed Also available for quantitative DIG ABA (c) Biotin UV-traceable antibody biotinylation Iron magnetite central layer Fast (< 2 min) magnetic response time 15X Quantitation is Built Into the Reagent 1X X Control IgG Versatile: labels 25 µg 1 mg Easy-to-use protocols on a flash drive Unique streptavidin/bead crosslinker technology By simply measuring the 28 and 354 of a modified protein, the concentration and number of biotins incorporated per protein can be determined directly. Description ChromaLink Biotin Labeling Kit Easy quantification, no ABA assay necessary ChromaLink ne-shot Antibody Biotinylation Kit ew size for quick antibody labeling ChromaLink Digoxigenin ne- nly traceable DIG Shot Antibody Labeling Kit reagent available VWR Cat. o Encapsulated (no exposed iron) monodispersed microspheres Kit labels 5 x 1 mg protein Kit labels 1 µg of Ab Kit labels 1 µg of Ab anolink Advantage: Competitive Landscape of Free-Biotin Binding Capacity 12, 1, 8, 6, 4, 2, anolink A B anolink (.8 mm) Binding Competitor s (1 mm) Binding >14, pmol/mg > 1,3 pmol/mg >2.5 nmol/mg A Biotinylated IgG (3 biotins per IgG) 25 mg/mg 2 mg/mg MagnaLink (2.8 mm) Binding Competitor s (2.8 mm) Binding >12, pmol/mg 65 9 pmol/mg >.8 nmol/mg A Biotinylated IgG (4 biotins per IgG) mg/mg 5 1 mg/mg C The Solulink conjugation process summarized above shows the conjugation of two amine-containing biomolecules. nce modification is complete, simple mixing of the two biomolecules allows the two linkers to selectively react with each other, forming the product. Easy to use 4FB Linker Activated Beads anolink 4FB beads are ideal for direct covalent immobilization of any yic-modified biomolecule (yic = aromatic hydrazine,) including yic-peptides, yic-oligonucleotides, and yicantibodies. Uniform size distribution Biotin Binding Capacity (pmol/mg).25 What Makes Solulink Crosslinking Technology Better? Flow cytometry Traceability The bond formed with yic and 4FB absorbs at 354 nm with a molar extinction coefficient of 29,. Reproducibility Biomolecule modification can be quantified for greater batch-to-batch reproducibility. Linker Stability Biomolecules modified with yic and 4FB have extended reaction stabilities. Bond Stability Bonds are stable to temperatures up to 94oC and a wide p range of p (p 2 11). Specificity Solulink crosslinking assures formation of heteroconjugates, with no formation of homoconjugates. Reactive linkers on modified biomolecules are inert and do not react with protein functional groups. Advantages of MagnaLink Magnetic Beads vs. the Competition anolink 85 nm particle MagnaLink uniform, 2.8 µm particle.3 Immunohistochemistry Used by research and commercial organizations worldwide to enable next-generation biomedical assays, detection systems, and protein arrays. Bulk quantities available. Consistent high-binding capacity (d) Long-chain PEG3 linker preserves (b) Traceable bis aryl hydrazone Streptavidin affinity and water solubility chromophore ( e = 354 nm) + Advantages of anolink Magnetic Beads vs. the Competition Polystyrene Core S3-a+ For use in: ELISA + Streptavidin Beads anolink and MagnaLink beads have the most consistent, ultra-high biotin binding of any particle on the market (>12 nmol/mg). Applications include capture and immobilization of biotinylated biomolecules, such as DA, RA, peptides, proteins and antibodies. (a) Water-soluble, succinimidyl ester S o ABA or fluoro-reporter assay uter core hydrophilic polymer surface Technology ChromaLink Biotin is the most advanced protein biotinylation reagent available. It features a water-soluble sulfo-succinimidyl ester functional group (a), which modifies protein lysine residues in an aqueous buffer. The linker is UV-traceable (b), and absorbs at 354 nm. This UV-signature permits rapid quantification of incorporated biotin (c), by means of nondestructive measurement (A28/A354 nm). The linker possesses an extended PEG3 spacer (d), which helps preserve and maintain streptavidin/biotin affinity while enhancing antibody solubility. Finally, a conjugation technology that is easy to use, is efficient, makes better conjugates, and is applicable to almost any conjugation project. Solulink technology is highly selective, and not susceptible to nonspecific binding, making it superior to conventional methods. For use in: Immunoprecipition Cell capture DA capture Covalently crosslinked streptavidin Scientific paper: UV-Traceable Reagent for Controlled Biotinylation of Antibodies BETTER RESULTS WIT EFFICIET, EASY-T-USE TECLGY Amino-modified Beads anolink amino-modified magnetic beads are for use with water-soluble linkers. VWR Cat. o. anolink Streptavidin Magnetic Beads,.8 µm anolink 4FB Magnetic Beads,.8 µm anolink Amino Magnetic Beads,.8 µm MagnaLink Streptavidin Magnetic Beads, 2.8 µm MagnaLink 4FB Magnetic Beads, 2.8 µm MagnaLink Amino Magnetic Beads, 2.8 µm Magnetic Stand holds one 1.5 ml tube Vendor >8% efficient conjugations Super stable conjugates 1x better stability than any other crosslinker Can crosslink any amine-containing biomolecules or surface Quantifiable conjugates o chromatography required VWR Cat. o. All-Purpose Crosslinking Kit with S-yic Kit Protein-Protein Conjugation Kit Kit 3 conjugations Protein-ligo Conjugation Kit Kit 2 conjugations RP-Antibody All-in-ne Conjugation Kit AP-Antibody All-in-ne Conjugation Kit R-PE-Antibody All-in-ne Conjugation Kit R-PE-Antibody Conjugation Kit APC-Antibody Conjugation Kit EW! Antibody-ligonucleotide Conjugation Kit Kit conjugates 1 µg Ab Solulink Antibody Biotin and Digoxigenin R-PE AP RP APC Fluorescein For more information visit VWR_US_print_cs_FEB14211-vwr-revisions.indd 4-6 2/18/211 2:44:12 AM

3 Crosslinking and Antibody anolink and MagnaLink Magnetic Beads ChromaLink Biotin Labeling Kit LIKER ACTIVATED, IGEST BIDIG CAPACITY TE MARKET anolink and MagnaLink beads reduce the overall bead mass required to immobilize a biotinylated sample. This provides the benefits of both lower backgrounds of nonspecific binding and lower net costs. ChromaLink Biotin links traceable biotins on any amine-containing biomolecule or surface. The kit allows for the simple and direct UV quantification of total incorporated biotin on antibodies and other proteins. Learn more on our website: Complete Kit: All necessary reagents included Built-in Quantitative Signal: Incorporated biotin quantified by a simple absorbance 28 and 354 reading Versatile: Labels 25 µg to 1 mg of antibody Safe & Economical: Water-soluble biotin reagent, mild on antibodies; no organics needed Also available for quantitative DIG ABA (c) Biotin UV-traceable antibody biotinylation Iron magnetite central layer Fast (< 2 min) magnetic response time 15X Quantitation is Built Into the Reagent 1X X Control IgG Versatile: labels 25 µg 1 mg Easy-to-use protocols on a flash drive Unique streptavidin/bead crosslinker technology By simply measuring the 28 and 354 of a modified protein, the concentration and number of biotins incorporated per protein can be determined directly. Description ChromaLink Biotin Labeling Kit Easy quantification, no ABA assay necessary ChromaLink ne-shot Antibody Biotinylation Kit ew size for quick antibody labeling ChromaLink Digoxigenin ne- nly traceable DIG Shot Antibody Labeling Kit reagent available VWR Cat. o Encapsulated (no exposed iron) monodispersed microspheres Kit labels 5 x 1 mg protein Kit labels 1 µg of Ab Kit labels 1 µg of Ab anolink Advantage: Competitive Landscape of Free-Biotin Binding Capacity 12, 1, 8, 6, 4, 2, anolink A B anolink (.8 mm) Binding Competitor s (1 mm) Binding >14, pmol/mg > 1,3 pmol/mg >2.5 nmol/mg A Biotinylated IgG (3 biotins per IgG) 25 mg/mg 2 mg/mg MagnaLink (2.8 mm) Binding Competitor s (2.8 mm) Binding >12, pmol/mg 65 9 pmol/mg >.8 nmol/mg A Biotinylated IgG (4 biotins per IgG) mg/mg 5 1 mg/mg C The Solulink conjugation process summarized above shows the conjugation of two amine-containing biomolecules. nce modification is complete, simple mixing of the two biomolecules allows the two linkers to selectively react with each other, forming the product. Easy to use 4FB Linker Activated Beads anolink 4FB beads are ideal for direct covalent immobilization of any yic-modified biomolecule (yic = aromatic hydrazine,) including yic-peptides, yic-oligonucleotides, and yicantibodies. Uniform size distribution Biotin Binding Capacity (pmol/mg).25 What Makes Solulink Crosslinking Technology Better? Flow cytometry Traceability The bond formed with yic and 4FB absorbs at 354 nm with a molar extinction coefficient of 29,. Reproducibility Biomolecule modification can be quantified for greater batch-to-batch reproducibility. Linker Stability Biomolecules modified with yic and 4FB have extended reaction stabilities. Bond Stability Bonds are stable to temperatures up to 94oC and a wide p range of p (p 2 11). Specificity Solulink crosslinking assures formation of heteroconjugates, with no formation of homoconjugates. Reactive linkers on modified biomolecules are inert and do not react with protein functional groups. Advantages of MagnaLink Magnetic Beads vs. the Competition anolink 85 nm particle MagnaLink uniform, 2.8 µm particle.3 Immunohistochemistry Used by research and commercial organizations worldwide to enable next-generation biomedical assays, detection systems, and protein arrays. Bulk quantities available. Consistent high-binding capacity (d) Long-chain PEG3 linker preserves (b) Traceable bis aryl hydrazone Streptavidin affinity and water solubility chromophore ( e = 354 nm) + Advantages of anolink Magnetic Beads vs. the Competition Polystyrene Core S3-a+ For use in: ELISA + Streptavidin Beads anolink and MagnaLink beads have the most consistent, ultra-high biotin binding of any particle on the market (>12 nmol/mg). Applications include capture and immobilization of biotinylated biomolecules, such as DA, RA, peptides, proteins and antibodies. (a) Water-soluble, succinimidyl ester S o ABA or fluoro-reporter assay uter core hydrophilic polymer surface Technology ChromaLink Biotin is the most advanced protein biotinylation reagent available. It features a water-soluble sulfo-succinimidyl ester functional group (a), which modifies protein lysine residues in an aqueous buffer. The linker is UV-traceable (b), and absorbs at 354 nm. This UV-signature permits rapid quantification of incorporated biotin (c), by means of nondestructive measurement (A28/A354 nm). The linker possesses an extended PEG3 spacer (d), which helps preserve and maintain streptavidin/biotin affinity while enhancing antibody solubility. Finally, a conjugation technology that is easy to use, is efficient, makes better conjugates, and is applicable to almost any conjugation project. Solulink technology is highly selective, and not susceptible to nonspecific binding, making it superior to conventional methods. For use in: Immunoprecipition Cell capture DA capture Covalently crosslinked streptavidin Scientific paper: UV-Traceable Reagent for Controlled Biotinylation of Antibodies BETTER RESULTS WIT EFFICIET, EASY-T-USE TECLGY Amino-modified Beads anolink amino-modified magnetic beads are for use with water-soluble linkers. VWR Cat. o. anolink Streptavidin Magnetic Beads,.8 µm anolink 4FB Magnetic Beads,.8 µm anolink Amino Magnetic Beads,.8 µm MagnaLink Streptavidin Magnetic Beads, 2.8 µm MagnaLink 4FB Magnetic Beads, 2.8 µm MagnaLink Amino Magnetic Beads, 2.8 µm Magnetic Stand holds one 1.5 ml tube Vendor >8% efficient conjugations Super stable conjugates 1x better stability than any other crosslinker Can crosslink any amine-containing biomolecules or surface Quantifiable conjugates o chromatography required VWR Cat. o. All-Purpose Crosslinking Kit with S-yic Kit Protein-Protein Conjugation Kit Kit 3 conjugations Protein-ligo Conjugation Kit Kit 2 conjugations RP-Antibody All-in-ne Conjugation Kit AP-Antibody All-in-ne Conjugation Kit R-PE-Antibody All-in-ne Conjugation Kit R-PE-Antibody Conjugation Kit APC-Antibody Conjugation Kit EW! Antibody-ligonucleotide Conjugation Kit Kit conjugates 1 µg Ab Solulink Antibody Biotin and Digoxigenin R-PE AP RP APC Fluorescein For more information visit VWR_US_print_cs_FEB14211-vwr-revisions.indd 4-6 2/18/211 2:44:12 AM

4 Crosslinking and Antibody anolink and MagnaLink Magnetic Beads ChromaLink Biotin Labeling Kit LIKER ACTIVATED, IGEST BIDIG CAPACITY TE MARKET anolink and MagnaLink beads reduce the overall bead mass required to immobilize a biotinylated sample. This provides the benefits of both lower backgrounds of nonspecific binding and lower net costs. ChromaLink Biotin links traceable biotins on any amine-containing biomolecule or surface. The kit allows for the simple and direct UV quantification of total incorporated biotin on antibodies and other proteins. Learn more on our website: Complete Kit: All necessary reagents included Built-in Quantitative Signal: Incorporated biotin quantified by a simple absorbance 28 and 354 reading Versatile: Labels 25 µg to 1 mg of antibody Safe & Economical: Water-soluble biotin reagent, mild on antibodies; no organics needed Also available for quantitative DIG ABA (c) Biotin UV-traceable antibody biotinylation Iron magnetite central layer Fast (< 2 min) magnetic response time 15X Quantitation is Built Into the Reagent 1X X Control IgG Versatile: labels 25 µg 1 mg Easy-to-use protocols on a flash drive Unique streptavidin/bead crosslinker technology By simply measuring the 28 and 354 of a modified protein, the concentration and number of biotins incorporated per protein can be determined directly. Description ChromaLink Biotin Labeling Kit Easy quantification, no ABA assay necessary ChromaLink ne-shot Antibody Biotinylation Kit ew size for quick antibody labeling ChromaLink Digoxigenin ne- nly traceable DIG Shot Antibody Labeling Kit reagent available VWR Cat. o Encapsulated (no exposed iron) monodispersed microspheres Kit labels 5 x 1 mg protein Kit labels 1 µg of Ab Kit labels 1 µg of Ab anolink Advantage: Competitive Landscape of Free-Biotin Binding Capacity 12, 1, 8, 6, 4, 2, anolink A B anolink (.8 mm) Binding Competitor s (1 mm) Binding >14, pmol/mg > 1,3 pmol/mg >2.5 nmol/mg A Biotinylated IgG (3 biotins per IgG) 25 mg/mg 2 mg/mg MagnaLink (2.8 mm) Binding Competitor s (2.8 mm) Binding >12, pmol/mg 65 9 pmol/mg >.8 nmol/mg A Biotinylated IgG (4 biotins per IgG) mg/mg 5 1 mg/mg C The Solulink conjugation process summarized above shows the conjugation of two amine-containing biomolecules. nce modification is complete, simple mixing of the two biomolecules allows the two linkers to selectively react with each other, forming the product. Easy to use 4FB Linker Activated Beads anolink 4FB beads are ideal for direct covalent immobilization of any yic-modified biomolecule (yic = aromatic hydrazine,) including yic-peptides, yic-oligonucleotides, and yicantibodies. Uniform size distribution Biotin Binding Capacity (pmol/mg).25 What Makes Solulink Crosslinking Technology Better? Flow cytometry Traceability The bond formed with yic and 4FB absorbs at 354 nm with a molar extinction coefficient of 29,. Reproducibility Biomolecule modification can be quantified for greater batch-to-batch reproducibility. Linker Stability Biomolecules modified with yic and 4FB have extended reaction stabilities. Bond Stability Bonds are stable to temperatures up to 94oC and a wide p range of p (p 2 11). Specificity Solulink crosslinking assures formation of heteroconjugates, with no formation of homoconjugates. Reactive linkers on modified biomolecules are inert and do not react with protein functional groups. Advantages of MagnaLink Magnetic Beads vs. the Competition anolink 85 nm particle MagnaLink uniform, 2.8 µm particle.3 Immunohistochemistry Used by research and commercial organizations worldwide to enable next-generation biomedical assays, detection systems, and protein arrays. Bulk quantities available. Consistent high-binding capacity (d) Long-chain PEG3 linker preserves (b) Traceable bis aryl hydrazone Streptavidin affinity and water solubility chromophore ( e = 354 nm) + Advantages of anolink Magnetic Beads vs. the Competition Polystyrene Core S3-a+ For use in: ELISA + Streptavidin Beads anolink and MagnaLink beads have the most consistent, ultra-high biotin binding of any particle on the market (>12 nmol/mg). Applications include capture and immobilization of biotinylated biomolecules, such as DA, RA, peptides, proteins and antibodies. (a) Water-soluble, succinimidyl ester S o ABA or fluoro-reporter assay uter core hydrophilic polymer surface Technology ChromaLink Biotin is the most advanced protein biotinylation reagent available. It features a water-soluble sulfo-succinimidyl ester functional group (a), which modifies protein lysine residues in an aqueous buffer. The linker is UV-traceable (b), and absorbs at 354 nm. This UV-signature permits rapid quantification of incorporated biotin (c), by means of nondestructive measurement (A28/A354 nm). The linker possesses an extended PEG3 spacer (d), which helps preserve and maintain streptavidin/biotin affinity while enhancing antibody solubility. Finally, a conjugation technology that is easy to use, is efficient, makes better conjugates, and is applicable to almost any conjugation project. Solulink technology is highly selective, and not susceptible to nonspecific binding, making it superior to conventional methods. For use in: Immunoprecipition Cell capture DA capture Covalently crosslinked streptavidin Scientific paper: UV-Traceable Reagent for Controlled Biotinylation of Antibodies BETTER RESULTS WIT EFFICIET, EASY-T-USE TECLGY Amino-modified Beads anolink amino-modified magnetic beads are for use with water-soluble linkers. VWR Cat. o. anolink Streptavidin Magnetic Beads,.8 µm anolink 4FB Magnetic Beads,.8 µm anolink Amino Magnetic Beads,.8 µm MagnaLink Streptavidin Magnetic Beads, 2.8 µm MagnaLink 4FB Magnetic Beads, 2.8 µm MagnaLink Amino Magnetic Beads, 2.8 µm Magnetic Stand holds one 1.5 ml tube Vendor >8% efficient conjugations Super stable conjugates 1x better stability than any other crosslinker Can crosslink any amine-containing biomolecules or surface Quantifiable conjugates o chromatography required VWR Cat. o. All-Purpose Crosslinking Kit with S-yic Kit Protein-Protein Conjugation Kit Kit 3 conjugations Protein-ligo Conjugation Kit Kit 2 conjugations RP-Antibody All-in-ne Conjugation Kit AP-Antibody All-in-ne Conjugation Kit R-PE-Antibody All-in-ne Conjugation Kit R-PE-Antibody Conjugation Kit APC-Antibody Conjugation Kit EW! Antibody-ligonucleotide Conjugation Kit Kit conjugates 1 µg Ab Solulink Antibody Biotin and Digoxigenin R-PE AP RP APC Fluorescein For more information visit VWR_US_print_cs_FEB14211-vwr-revisions.indd 4-6 2/18/211 2:44:12 AM

5 Solulink s superior crosslinking technology gives you tools that are: More quantifiable More efficient Easier to use More versatile igher yielding GET BETTER RESULTS. GUARATEED. ChromaLink Biotinylation Kits ChromaLink Biotin allows for the simple and direct spectroscopic quantification of incorporated biotin. Complete kit Built-in quantitative signal Labels 25 µg to 1 mg Safe and economical anolink and MagnaLink Magnetic Beads ighest Binding Capacity on the Market: >12 nmol/mg Solulink s anolink and MagnaLink Magnetic Beads possess the highest biotin-binding capacity of any commercially available polymerencapsulated particle. igh surface area igh binding capacity Fast magnetic response time.8 µm and 2.8 µm sizes Crosslinking and Antibody Stable, high specificity provides conjugate yields >8% Solulink s technology is highly selective, stable in solution, and not susceptible to nonspecific binding, making it superior to conventional methods. UV-traceable technology Stable conjugate bond Crosslinks any biomolecule Efficient and reproducible o chromatography required for All-in-ne Antibody To order visit

6 rder Today 1% Satisfaction Guarantee Exceptional quality is of paramount importance to us. To ensure absolute consistency, each batch of every product must pass our stringent biochemical and biological/immunological testing requirements. ChromaLink Biotin and DIG VWR Cat. o. Price ChromaLink Biotin Labeling Kit, Flash drive Kit labels 5 x 1 mg protein $325 ChromaLink ne-shot Antibody Biotinylation Kit Kit labels 1 µg of Ab $15 ChromaLink Digoxigenin ne-shot Antibody Labeling Kit Kit labels 1 µg of Ab $195 Magnetic Beads VWR Cat. o. Price anolink Streptavidin Magnetic Beads,.8 µm $25 anolink 4FB Magnetic Beads,.8 µm $25 anolink Amino Magnetic Beads,.8 µm $5 MagnaLink Streptavidin Magnetic Beads, 2.8 µm $295 MagnaLink 4FB Magnetic Beads, 2.8 µm $295 MagnaLink Amino Magnetic Beads, 2.8 µm $12 Magnetic Stand holds one 1.5 ml tube $75 Bead Block ml $5 *Please inquire for orders >1 L Crosslinking and Antibody VWR Cat. o. Price All-Purpose Crosslinking Kit with S-yic Kit includes 1 mg of reagent $35 Protein-Protein Conjugation Kit Kit 3 conjugations $395 Protein-ligo Conjugation Kit Kit 2 conjugations $395 RP-Antibody All-in-ne Conjugation Kit $395 AP-Antibody All-in-ne Conjugation Kit $395 R-PE Antibody All-in-ne Conjugation Kit Kit conjugates 2 x 1 µg $395 R-PE-Antibody Conjugation Kit $395 APC-Antibody Conjugation Kit $395 EW Antibody-ligonucleotide All-in-ne Conjugation Kit Kit conjugates 1 µg of Ab $425 *Please inquire for bulk kit orders 211 3M Lit. o. 1957