Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-Upon-Thames, TW18 3HR, UK

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1 Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-Upon-Thames, TW18 3HR, UK Issue No: 040 Issue date: 30 ugust 2018 ccredited to The Cefas Weymouth Laboratory The Nothe Barrack Road Weymouth DT4 8UB Contact: Miss D Page Tel: +44 (0) Fax: +44 (0) debbie.page@cefas.co.uk Website: Laboratory locations: below s covered by the organisation and their relevant activities details ctivity code ddress CEFS Weymouth Laboratory Barrack Road The Nothe Weymouth Dorset DT4 8UB Local contact Miss D Page Tel: +44 (0) Fax: +44 (0) debbie.page@cefas.co.uk Website: ll Site activities performed away from the locations listed above: details ctivity code Fish Farms Sampling of fish tissues for subsequent virological and bacterial testing B ssessment Manager: KH Page 1 of 7

2 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 DETIL OF CCREDITTION Chemical nalysis Documented in-house methods: FOOD ND FOOD - BIVLVE MOLLUSCS Determination of Domoic acid (amnesic shellfish poisoning toxin, SP), SOPs 1180, 1744, 1200, 1243 and 1723, by HPLC-UV Mussels, cockles, Pacific oysters, native oysters, scallops (whole king, whole queen and processed king) and clams (razor, hard, surf, manila, otter and carpet shell) pre-shucked king scallops (adductor only and adductor roe) Paralytic shellfish poisoning toxin (PSP): Semi-quantitative screening and quantitative test SOPs 1180, 1744, 1784, 1785, 1788 and 2168 by HPLC-FLD based on OC Official method Mussels, cockles, Pacific Oysters, Native oysters, scallops (whole King, whole queen and processed king) and clams (razor, surf, manila, otter and hard) Lipophilic Marine Toxins (DSP) : Quantitative test SOPs 1180, , 2099 and 2101 by LC-MS/MS Fresh water, algae, bluegreen algae food supplements (tablets or powder) and shell fish Determination of Cyanotoxins: Quantitative test MC-RR MC-L MC-LY MC-LF MC-LW MC-YR MC-WR MC-HilR MC-HtyR MC-LR sp 3 -MC-LR sp 3 -MC-RR Nodularin SOP 2308 by LC-MS/MS Molecular Biology Bivalve mollusc digestive gland Quantification of norovirus genogroup I and II RN SOP 1330 by real-time reverse transcriptase PCR using Mx3005P Stratagene thermocycler ssessment Manager: KH Page 2 of 7

3 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 FOOD ND FOOD - BIVLVE MOLLUSCS Molecular Biology Bivalve Mollusc Digestive Gland Quantification of Hepatitis virus RN SOP 1330 using real-time RT-PCR based on ISO :2017 by TaqMan chemistry using the Stratagene Mx3005P thermal cycler Crustacean Tissue white spot syndrome virus (WSSV) DN (absence or presence) SOP 2076 by robotic extraction (Qiagen BioRobot EZ1 or EZ1 advanced XL) with Qiagen DN tissue kit followed by conventional PCR as stated in the OIE manual of Diagnostic Tests for quatic nimals. Products direct sequenced using the BI Bg Dye Terminator Kit v3.1 and BI 3500xl genetic analyser Fish Tissues Cyprinid Herpes virus type III (CyHV-3) DN (absence or presence) SOP 2152 Prepared by robotic Virus mini kit, followed by a published PCR method. Products direct sequenced using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. Fish Tissues Spring Viraemia of Carp virus (SVCV) RN (absence or presence) SOPs 2063, Prepared by robotic or EZ1 dvanced XL) with Qiagen Virus mini kit, followed by conventional PCR as stated in the OIE manual of Diagnostic Tests for quatic nimals. Products direct sequenced using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. ssessment Manager: KH Page 3 of 7

4 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 FOOD ND FOOD - BIVLVE MOLLUSCS Molecular Biology Fish Tissues Koi Herpes virus (KHV) DN (absence or presence) SOPs Prepared by robotic Virus mini kit, followed by conventional PCR as stated in the OIE manual of Diagnostic Tests for quatic nimals. Followed by a nested PCR designed in house. Products direct sequencing using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. Fish tissues Viral Haemorrhagic septicaemia virus (VHSV) RN (absence or presence) SOP 2108 Prepared by robotic Virus mini kit, followed by published conventional RT-PCR methods. Products direct sequencing using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. Fish tissues Infectious Haematopoietic Necrosis virus (IHNV) RN (absence or presence) SOP Prepared by robotic Virus mini kit, followed by conventional RT-PCR as stated in the OIE manual of Diagnostic Tests for quatic nimals. Products direct sequencing using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. Crustacean tissues Yellow Head Virus (YHV) RN (absence or presence) SOP Prepared by robotic RN Tissue kit, followed by conventional RT-PCR as stated in the OIE manual of Diagnostic Tests for quatic nimals. Products direct sequencing using the BI Big Dye Terminator kit v3.1 and BI 3500xl genetic analyser. ssessment Manager: KH Page 4 of 7

5 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 FOOD ND FOOD - BIVLVE MOLLUSCS Molecular Biology Fish, Shellfish and Crustacean tissue Detection and identification of viruses, bacteria, fungi and parasites SOPs 2008, 2006, 2007,1537, 1742,1546 by robotic extraction, conventional PCR including RT- PCR and sequence analysis using BI 3130 analyser under Flexible Scope SOP 2064 FOOD ND FOOD Microbiological Tests - BIVLVE MOLLUSCS Enumeration: Escherichia coli Detection: Salmonella spp (excluding S. typhi) SOP 1175, based on ISO :2015 SOP 1176, based on ISO6579-1:2017 Vibrio parahaemolyticus SOP 1333, based on ISO : BIVLVE MOLLUSCS Bioassays FISH, Bivalve Mollusc and Crustacean tissues Sampling Fish For tissues submitted for virological, molecular, bacterial and histopathological diagnostic testing to undertake a full disease screen, including screening for all diseases affecting fish listed in Council Directive 2006/88/EC and the quatic nimal Health (England and Wales) Regulations 2009 SOP Fish sampling methodology in accordance with: Council Directive 2006/88/EC The quatic nimal Health (England and Wales) Regulations 2009, B ssessment Manager: KH Page 5 of 7

6 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 FISH, Bivalve Mollusc and Crustacean tissues Sampling Bivalve Mollusc For tissues submitted for histopathological diagnostic testing to undertake a full disease screen, including screening for all diseases affecting bivalve molluscs listed in Council Directive 2006/88/EC and the quatic nimal Health (England and Wales) Regulations 2009 SOP Mollusc Sampling Methodology In accordance with: Council Directive 2006/88/EC The quatic nimal Health (England and Wales) Regulations 2009, B Crustaceans For tissues and fluids submitted for molecular, bacterial and histopathological diagnostic testing to undertake a full disease screen, including screening for all diseases affecting crustaceans listed in Council Directive 2006/88/EC and the quatic nimal Health (England and Wales) Regulations 2009 SOP Crustacea Sampling Methodology In accordance with: Council Directive 2006/88/EC The quatic nimal Health (England and Wales) Regulations 2009, B Virological Tests Brain, Kidney and Spleen Viral Haemorrhagic Septicaemia virus (VHSV) SOP s 1248, 1279, 1125 and 1259 by tissue culture and ELIS in accordance with Commission Decision (EU) 2015/1554, Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for quatic nimals, 2009) Infectious Haematopoietic Necrosis virus (IHNV) SOP s 1248, 1279, 1125 and 1263 by tissue culture and IFT in accordance with Commission Decision (EU) 2015/1554, Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for quatic nimals, 2009) ssessment Manager: KH Page 6 of 7

7 Schedule of ccreditation United Kingdom ccreditation Service ccredited to Issue No: 040 Issue date: 30 ugust 2018 FISH, Bivalve Mollusc and Crustacean tissues Virological Tests Brain, Kidney and Spleen spring Viraemia of Carp (SVC) virus SOP s 1248, 1279, 1125 and 1259 by tissue culture and ELIS in accordance with Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for quatic nimals, 2009) Fish tissues (homogenate supernatants) Isolation of Epizootic haematopoietic necrosis virus (EHNV) SOP s 1248, 1279 and 1125 by tissue culture (BF2 and EPC cells) in accordance with Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for quatic nimals, 2009) Fish, shellfish, crustacean tissues Detection and Identification of Spring Viraemia of Carp virus (SVCV) RN SOPs 2063, 2008, 2006,2007, 1537, 1742 and 1546 by RT-PCR. Guidance from the quatic nimal Health Directive - Council Directive 2006/88 (based on OIE Manual of Diagnostic tests for quatic nimals 2006) Fish, shellfish, crustacean tissues Detection and Identification of Koi Herpes virus (KHV) DN SOPs 1493, 2008, 2006,2007, 1742 and 1546 by PCR. Guidance from the quatic nimal Health Directive - Council Directive 2006/88 (based on OIE Manual of Diagnostic tests for quatic nimals 2006) Fish tissue and homogenates Infectious Salmon naemia virus (ISV) SOPs 1248, 1279, 1125,1263 & 1268 by tissue culture (TO and SHK cells) and IFT in accordance with Commission Decision (EU) 2015/1554, Council Directive 2006/88/EC (based on OIE Manual of Diagnostic Tests for quatic nimals 2009) END ssessment Manager: KH Page 7 of 7