Ver.5 AccuPrep

Transcription

1 0808 Ver.5 AccuPrep Spin Column Type Nucleic Acid Preparation

2 Prepare High Purity, High Yield Nucleic Acid with AccuPrep Kit! AccuPrep series are s most popular nucleic acid extraction product which uses a single spin column or 96-well plate with glass micro fiber filters. provides various products for high-purity and high-yield genomic DNA, plasmid DNA, RNA or fragment DNA.

3 DNA Extraction Genomic DNA Extraction Kit AccuPrep Genomic DNA Extraction Kit can rapidly and conveniently extract genomic DNA from blood, lymphocyte, buffy coat, animal tissue and cultured cell. This process does not require phenol/chloroform extraction, alcohol precipitation, nor other burdensome steps. The isolated DNA is ready to use in various applications. Conc. A60/ Company Q *Elution Volume : 00 μl Figure. Genomic DNA extracted by AccuPrep Genomic DNA Extraction Kit gdna extraction from 30 mg of bovine tissue : kb ladder (D-040, ), :, : Company Q Plant Genomic DNA Extraction Kit AccuPrep Plant Genomic DNA Extraction Kit extracts DNA from plant samples including leaf and seeds. The kit includes all reagents required for extraction, and uses a column type extraction system to allow rapid, convenient extraction of higher-quality DNA. Conc. A60/ Company Q *Elution Volume : 00 μl Figure. Genomic DNA extracted by AccuPrep Plant Genomic DNA Extraction Kit gdna extraction from 00 mg of Brassica oleracea var. italica : kb ladder (D-040, ), :, : Company Q Stool DNA Extraction Kit AccuPrep Stool DNA Extraction Kit extracts DNA from stool or other samples containing large amounts of material that can inhibit PCR. Using the spin-column method, contaminants and enzyme inhibitors such as heparin, bilirubin bile salts are eliminated and highpurity DNA is obtained. (A) 3 (B) 3 4 Figure 3. Stool genomic DNA extracted by AccuPrep Stool DNA Extraction Kit (A), PCR results of H. pylori DNA extracted by AccuPrep Stool DNA Extraction Kit (B) (A) : kb ladder (D-040, ), Lane -3: 00 ng of Intact DNA (left) and Hind III enzyme digested DNA (right) (B) : 00 bp ladder (D-030, ), Lane -4: PCR results for H. pylori detection Specification & Application Product Sample Expected yield Prep. time Application Genomic DNA Kit Plant Genomic DNA Kit Stool DNA Kit Whole blood Buffy coat Cultured cells ammalian tissue Soybean aize Potato Stool 3-6 μg 5-0 μg 5-0 μg 0-5 μg -5 μg -4 μg -4 μg -5 μg < 50 min Gene cloning, PCR, Real-time PCR, Southern blotting, and SNP genotyping < 50 min

4 Nano-Plus Plasmid Extraction Kit AccuPrep Nano-Plus Plasmid Extraction Kit extracts high quality plasmid DNA from cultured bacterial cells. The kit reduces plasmid DNA prep time from the usual 30 minutes to 0 minutes. The overall principle utilizes a modified alkaline lysis protocol that is enhanced by patented s Nano-Technology. Company P Company Q Plasmid ini Extraction Kit Conc. A60/80 AccuPrep Plasmid Extraction Kit extracts high-purity Company Q binding/washing/elution. Dangerous organic solvents are avoided for the safe and convenient extraction of plasmid DNA *Elution Volume : 00 μl Figure. Plasmid DNA extracted by AccuPrep Plasmid Extraction Kit 9 Extraction of pge -T easy vector from 3.6 x 0 E. coli DH5α cells : kb ladder (D-040, ), :, : Company Q PCR/Gel DNA Purification Kit AccuPrep PCR/Gel Purification Kit purifies DNA fragment from PCR or other enzymatic products as well as agarose gel. 70 bp-0 kb size of fragment DNA can be purified with 70-90% recovery, and common 0-40 mer short oligonucleotides are easily removed. Specification & Application Nano-Plus Plasmid Extraction Kit ini idi axi Plasmid ini Extraction Kit Up to 0 μg Up to μg Up to μg Up to 0 μg Variable < 0 min < 40 min < 60 min Product Expected yield Prep. time Application Sub-cloning, sequencing, transformation, transfection, and in-vitro transcription/translation PCR/Gel DNA Purification Kit Sub-cloning, sequencing, labeling, and DNA concentration

5 RNA Extraction AccuPrep extracts high-quality RNA from various type of samples such as cultured animal cell, animal tissue and plant tissue by common extraction kit. Conc. A60/ Company Q Conc. A60/ Company Q *Elution Volume : 00 μl Conc. A60/ Company Q *Elution Volume : 00 μl *Elution Volume : 00 μl Figure. Cultured cell RNA extracted by AccuPrep RNA extraction from 06 of HeLa cell. DNase was not treated. Figure. Plant RNA extracted by AccuPrep RNA extraction from 00 mg of Brassica oleracea var. italica. DNase was not treated. Figure 3. Animal tissue RNA extracted by AccuPrep RNA extraction from 0 mg of us musculus liver. DNase was not treated. :, : Company Q :, : Company Q :, : Company Q Viral RNA Extraction Kit AccuPrep Viral RNA Extraction Kit extracts viral RNA from cell-free samples as serum, plasma, CSF, urine, etc. This kit can be used for extracting RNA from a variety of RNA virus, but not all viruses can be utilized as a source. Also, as it is difficult to extract only RNA from samples containing both DNA and RNA, a cell-free sample must be used in order to prevent DNA contamination. Specification & Application Product Viral RNA Extraction Kit Cultured animal cell, mammalian tissue, plant tissue Viral RNA in body fluid (blood, plasma, serum and urine) Starting volume Variable Variable Column binding capacity RT-PCR, RT-qPCR, cdna synthesis, northern/dot/slot blot analysis, microarray, and RNAseq RT-PCR, RT-qPCR, cdna synthesis, poly A+ RNA selection, northern/dot/slot blot analysis, and pathogen detection Sample Application

6 Ordering Information Cat. no. Products K-3 AccuPrep Nano-Plus Plasmid ini Extraction Kit, 00 reactions K-3 AccuPrep Nano-Plus Plasmid ini Extraction Kit, 50 reactions K-3 AccuPrep Nano-Plus Plasmid idi Extraction Kit, 5 reactions K-33 AccuPrep Nano-Plus Plasmid axi Extraction Kit, 5 reactions K-33 AccuPrep Nano-Plus Plasmid axi Extraction Kit, 0 reactions K-3030 AccuPrep Plasmid Extraction Kit, 00 extractions K AccuPrep Plasmid Extraction Kit, 50 extractions K-303 AccuPrep Genomic DNA Extraction Kit, 00 extractions K-303 AccuPrep Plant Genomic DNA Extraction Kit, 00 extractions K-3036 AccuPrep Stool DNA Extraction Kit, 00 extractions K-340 AccuPrep, 00 extractions K-34 AccuPrep, 50 extractions K-3033 AccuPrep Viral RNA Extraction Kit, 00 extractions K-3037 AccuPrep PCR/Gel DNA Purification Kit, 00 purifications K-3038 AccuPrep PCR/Gel DNA Kit, 50 purifications K AccuPrep Plasmid DNA Extraction Kit for 96 well vacuum Block, 96-well plate x K-303- AccuPrep Genomic DNA Extraction Kit for 96 well vacuum Block, 96-well plate x K AccuPrep PCR Purification Kit for 96 well vacuum Block, 96-well plate x Contact Us Corporation Inc. R&D Center 8- unpyeongseo-ro, Daedeok-gu Daejeon, 3430, Republic of Korea Tel: (Korea: ) Fax: sales@bioneer.com 30 arina Village PKWY, Suite 0 Alameda, CA 9450, USA Toll Free: Fax: order.usa@bioneer.us.com Korea Bio Park BLDG #B Daewangpangyo-ro, Bundang-gu, Seongnam-si Gyeonggi-do, 3488, Republic of Korea Tel: Fax: AccuPrep is a trademark of Corporation. Copyright 08. Corporation. All Rights Reserved.