Liquid Chromatography 3000 Series

Transcription

1 Liquid Chromatography 3000 Series 3000 Series Inteigent HPLC / nano HPLC PRER. & Production HPLC Anaytica HPLC Anaytica Technooies Limited Products Appications Software Accessories Consumabes Services

2 2 APPLICATIONS HPLC anaytica usuay appied to different poarirty invoatie thermostabe organic compounds, aso a variety of bioactive substances and natura products:synthetic and natura poymers amongst many. Today, 80% of the organic compounds can use iquid chromatography for anaysis and detection. Pharmaceuticas & Medica Drug anaysis for pharmacy, detections of effective components, drug metaboite contro, micro toxin in-vivo anaysis and microbia drug anaysis. Heath & Epidemic prevention Cinica anaysis, research of disease contro, micro anaysis in bioogica areas, human biochemica anaysis, and metaboite anaysis. Environmenta Monitoring Water, air, rainfa monitoring and determination of the content of various poutants. Agricuture, forestry, fisheries, anima hasbandry Pesticide residue detection, crop detection, chemica fertiiser detection, pant quarantine, veterinary drug detection, aquamarine detection. 14 DNPH Adosterone Derivatoves Anaysis Mobie phase: Water/Acetonitrie=40/60 Waveength: 360nm Fow Rate: 1m/min Tempreture:ambient Bacicain Anaysis See Pharmacopoeia 2005 (first edition)

3 Bio-pharmaceuticas & Fine Chemicas Process HPLC S are used for production of Phyo & fine Chemicas etc. Manufacturing Process contro and product testing, such as anaysis of food preservatives, sweeteners, spices, food enzyme, carbohydrate, vitamins, nutrients, cosmetics preservatives and antimicrobia agent detection. Patform Hoy Oi Reservoir sampe (we# ) Petrochemica Industria process contro, product testing and manufacturing processes. Jackpot seep Oi Emerrgiing Through Seafoor 10 9 Time (seconds) 2 Dimension Retention Based Upon Poarity nd Time (minutes) st Dimension Retention Based Upon Boiing Point Quaity Contro Quaity contro of commodity inspection,quaity inspection, import/export quarantine departments. Education and Scientific Research Educationa estabishments, institution for experiment, scientific research,teaching and demonstration. Water Conservation System Water quaity and environmenta monitoring, fresh water and sewage treatment pants. Other Areas Power station,miitary,judicia,pubic security detection and forensics amongst others. Hydrophiic Vitamins Anaysis Mobie phase: 0.1% TF A/Acetonitrie =40/60 Waveength: 280nm Fow Rate: 1m/min, Temperature: 30 o

4 4 Typica Configurations for Various Appications Productions and Process HPLC Isocratic HPLC Binary HPLC UV Preparative Process HPLC Automomated GPC system/ PDA Amino Acid Anayzer Quatemary With Gradient with Auto Samper Ion Chomatograph Features Accurate Accuracy is the spirit of the anaysis. Based on a we structured design and manufacturing process, unquestionabe accuracy and precision were a prerequisite, secondty quantification and precision of anaysis were dependent on accurate fow rate. Stabiity Exceent reiabiity and stabiity. The pump heads have been machined using a first cass CNC process the innovative structura design has been designed to achieve minimum pressure fuctuation. Consequenty stabe fow, ow noise and over a quaity provides the foundation for reiabe resuts. Powerfu Anachrom software offers compete contro, powerfu data processing, reporting functions, inteigent diagnosic systems and maintenance utiity ensure a compete and fexibe software soution for your anaytica work. Beautifu Art combined with practicay. The moduar system provides shape and character whist being aestheticay peasing the unique and practica design ensures ease of use and maintenance. In essence, the combination of art and praticay compiment each other. Economica The inteigent HPLC is a high perfomance yet cost effective soution.the moduar design ensures that you can configure the system to meet your appication requirements. A dedicated team of product speciaists wi provide technica support and provide an appication deveopment service.

5 11 5 Pumps 3113i Isocratic HPLC Pump The 3113i Isocratic Pump is a robust, ow pusation sovent deivery system. The pumphead is easiy accessibe from the front pane to make routine maintenance, ike changing pump seas, easy and fast. The system is avaiabe with Micro, Anaytica or Semi Preparative pumphead in Stainess Stee or PEEK. 3113i Low-Pressure Quaternary Gradient Pump The 3113i Low-Pressure Quaternary Gradient Pump incorporates an active ow pressure mixer with adjustabe mixing voume. The mixing chamber voume can be freey adjusted. An optiona integrated vacuum degasser removes dissoved gases in the euents and prevents air bubbes in the system. The system is avaiabe with Micro, Anaytica or Semi Preparative pumphead in Stainess Stee or PEEK. 3132i High-Pressure Binary Gradient Pump The 3132i High-Pressure Binary Gradient Pump incorporates two pump systems with active high pressure mixer with adjustabe chamber voume. An optiona integrated vacuum degasser removes dissoved gases in the euents and prevents air bubbes in the system. The system is avaiabe with Micro, Anaytica or Semi Preparative pumpheads in Stainess Stee or PEEK. Technica Specifications 3113i Quatenary Gradient Pump Wetted Materias: Fow rate: Fow Accuracy: Fow Precision: Pressure Range: Pressure: Pusation: Compressibiity: Compensation: Dimensions: (W x H x D) Power Suppy: Stainess Stee / PEEK*, Tefon AF PVDF, Ceramics, Sapphire, Ruby Programmabe Micro: m/min Anaytica: m/min Semi-Preparative: m/min Semi-Preparative: mL/min ± 0.08% m / min ± 0.1 % RSD m/min psi Semi-Preparative: 20 MPa (up to m/min); 10 MPa (up to m/min) typica < 0.1 MPa or < 1.0 % user-adjustabe for different sovents 396 x 250 x 478 mm ~V (47-63 Hz) Vacuum Degassing: Gradient Range: Gradiant Accuracy: Gradiant Mixing: Mixing Voume: Composition Precision: 3113i Binary Gradient Pump Vacuum Degassing: Gradient Range: Gradiant Accuracy: Gradiant Mixing: Mixing Voume: optiona: < 20% dissoved gases remaining in m/ min %, 4 channes < 0.50 % Active adjustabe: µ < 0.15% RSD optiona: < 20% dissoved gases remaining in m/ min %, 4 channes < 0.50 % Active adjustabe: µ * depending on configuration

6 6 Product Specification P6000 Preparative HPLC Pump Anaytica's preparative HPLC pump is doube piston design. Deveoped server engine. Exported check vave and seaing ring. Providing high quaiity products for the customer. Technica Specification Deivery method doube pistons, main & auxiiary Contro Stand-aong mode by front keypad Fow rate accuracy + 0.1% - HPG Mode: one pump work as master, Fow rate Precision < 0.5%RSD - others work as save Dispay Graphics & LCD 320X240 bue background Coour Rs232 by PC using communication Protection Pmax & Pmin Data automaticay saved after power protoco down Power Suppy 220V±10%, 50Hz, 400W System connection 1/8"' capiaries, 1/4"' capiaries HPG up to Quaternary HPG GLP Record running time automaticay Product Specification 3000 Pus High Pressure UHPLC Pump The High Pressure UHPLC Pump has been specificay designed for Anaytica to high speed, high resoution Chromatography appications. Aso, a constant pressure version has been deveoped to aow packing of partice in the 1 micron to 10 micron range. The Anaytica Pump provides fow rates from to m/min, with fow settings in 1 L increments Puse-free fow in the Anaytica Pump is guaranteed by using advanced sovent recompression agorithms and automatic pressure compensation which provides unmatched performance for the most demanding appications.the Packing Pump has fow range settings from to 12mL/min with fow controed by the pressure setting. The High Pressure UHPLC Pump is offered as Technica Specifications. Technica Specification Fow Rates ml/min mL/min Pressure psi 12,000 psi (@0.01-5m/min) (Optiona) 18,000 psi (@0-2mL/min) (Optiona) Pusation % from 3000 to psi Pressure Accuracy...+ 2% of fu scae pressure Sovent degassing...buit in 4 channes Gradient mixer...active Fow Accuracy... < + 1% at 1 ml/min System dwe voume...< 400u Fow Precision % RSD on > 30 sec.average fow Composition Accuracy % Composition Precision...<0.15% RSD Physica H x W x 17.5 D (14 x 26 x 44 cm) Euent Degassing... onine membrane Degasser Gradient range %, 4 channes

7 High Pressure HPLC Pump This new powerfu pump is a new member of P6000 pumps such as great powerfu, the ong ifetime, the very high fow rate accuracy, fow rate precision, speciay suit for the industria production. P equipped with servomotors. The two pistons working in parae mode to reduce the pusation. The pump can be configured as HPG system and contro a procedure from injection to target fractions by the software Anachrom. Technica Specification Preparative HPLC Pump mL/min,10Mpa Dispay: LCD, 320x240 bue back ground Pump head: 3000mL coor Max. working Pressure: 10 Mpa Protection: Pmax &Pmin Date Automaticay Fow Range: mL/min. save after power down Fow rate Accuracy: Fow rate precision: Configuration <1% RSD <0.5% L HPLC pump 2 Piston for L 3 Piston sea for L 4 Specia cabe for Rs232 Connection Power suppy: 220V + 10%, 50Hz, 1000W Dimension & weight: 700 x 466 x 248, 40kg. Product Specification Large Scae High Pressure Piston Pump Internationa eve arge fow rate high pressure piston pump design, with deveoped driving system exported from oversea, a the materia which contacts the iquide is 316Lor non-meta materia, a this pump fit for the industria proparative HPLC system, with high fow rate accuaracy, itte pusation, the pump can work under muti kinds working conditions. Technica Specificaiton Liquid Deivery mode pump head Working Pressure Fow rate range Dispay Gradient Muti piston parae connection 316L Stainess Stee 10Mpa 10L/min - 85L/min LCD High Pressure Gradient or ow pressure gradient Configuration 1 P L Preparative HPLC pump 1 2 Piston for P L 1 3 Piston sea or P L 1 4 Specia cabe for Rs232 Connection 1

8 Product Specification Pneumatic Pump P6000pa Pneumatic sovent deivery system without eectronics for fow rates up to 1000mL/min. For hazardous environments. For constant pressure appications. For easy coumn packing up to 1000bars-deveoped for the production of high performance coumns, either anaytica or preparative. For preparative chromatography. For process chemistry. Technica Specificaiton 1 Pneumatic Pump P6000PA, 100m/min, Stainess stee 1 2 Pneumatic Pump P6000PA, 250m/min, Stainess stee 1 3 Pneumatic Pump P6000PA, 500m/min, Stainess stee 1 4 Pneumatic Pump P6000PA, 1000m/min, Stainess stee i Sampe Injector System The Anaytica 3530i Sampe Injector System is a very fexibe and powerfu HPLC autosamper with exceent reproducibiity and inearity properties. Variabe via racks and adaptors for microiter pates as we as a mutitude of firmware options make this system highy adaptabe and suitabe for any anaytica appication. Wetted Materias: Sampe Capacity: Stainess Stee / PEEK*, PPS, PVDF 120 (2 m), 192 (microtiter pates) Injection Voume: Programmabe µ Injection Voume Accuracy: Repicate Injection: µ 1-50 Per via Sampe Heating/Cooing: optiona: C Injection Precision: < 0.5 % Variabe Voume Injec tion (10 µ; typicay ~0.25 %) Linearity: Correation Factor > (10 µ injection voume, 500 µ Syringe) Cross contamination: <0.1% Wash with & without automated neede Wash Temperature Accuracy: C Carry Over: < 0.05 % with wash program Dimensions: (W x H x D) Power Suppy: 396 x 275 x 478 mm ~V (47-63 Hz) Dua-Neede Design The Dua-Neede design of the 3530i Sampe Injector System avoids system bockages due to septum partices injected into the system. The ventiation neede pierces the septum before the injection neede moves into the sampe via (see figure on the right). As the more fragie injection neede doesnot need to pierce the via septum, stronger via caps or pastic vias can be used without probems.

9 9 Product Specification 3325i UV/VIS Detector The Anaytica 3325i UV/Vis Detector is a variabe waveength UV/Vis detector for routine anaysis and sophisticated research. The dua amp design offers a waveength range of nm with a ow baseine noise. The front-accessibe fowce can be easiy exchanged, as we as the amps which are accessibe through a side pane in the instrument housing. Integrated Waveength Program The 3325i UV/Vis Detector features a waveength program to change the seected waveength over time. With this feature the optimum waveength can be seected for each anayzed substance according to its retention time. Integrated Peak Detector The integrated Peak Detector works as a basic fraction coector. The peak detection eve can be freey programmed for peak start and peak end to enhance the coection purity. An integrated 24V output for switching a soenoid vave is used for the fraction coection, which is automaticay operated with a seectabe time deay. Optiona - Dua - Waveength Technica Specifications The 3325i UV/Vis Detector is avaiabe with an optiona second waveength. This feature enhances the Waveength Program feature that you can measure 2 different waveengths at the same time. A second D/A converter output comes with this option to keep the system fexibe to be used with any data acquisition software avaiabe. Optiona - Onine-Scan Another option for the 3325i UV/Vis Detector is the Onine Scan. With the Onine Scan whoe spectrum information can be gathered at a certain time. This scan information is stored internay and can be accessed at any time. The Onine Scan is a good aternative to a fu UV PDA detector. Wetted Materias: Baseine Noise: Baseine Drift: Waveength Range: Waveength Accuracy: Linearity: Light Source Waveength Program: Stainess Stee / PEEK* ± 0.25x10-4 AU (@240 nm, 2 sec Risetime) < 1 x 10-4 AU/h nm ± 1 nm > 2.0 AU Deuterium Lamp, Tungsten Lamp Programmabe, 10 steps Anaog Output: 1x 1 V (optiona: 2x 1V) Contro Features: Interna Peak Detector with +24 V soenoid switching output. Dimensions: (W x H x D) Power Suppy: 396 x 165 x 478 mm ~V (47-63 Hz) 3325 Pus UV DETECTOR (Specia features for Bio-LC appication) Waveength range Band width Waveength accuracy Waveength reproducibiity Baseine noise Base ine draft Fow ce PH Sensor Range Conductivity sensor range nm, nm 8nm ± 2nm 0.4nm 3x10-5Au 1x10-4Au/h 1/16, PEEK, optica path 10mm 0 to 14 0 to 999ms.cm

10 10 Product Specification 3335i PDA Detector The Anaytica 3335i UV/Vis Detector is a photo diode-array (PDA) detector for routine anaysis and sophisticated research. The dua amp design offers a waveength range of nm (256 Diodes or nm (1024 diodes) with a ow baseine noise. The front-accessibe fowce can easiy be exchanged, as can be the amps which are accessibe through a side pane in the instrument housing. 4-Channe UV Detector The 3335i PDA Detector features 4-Waveength channes to measure chromatograms at 4 different waveengths at the same time. With this feature the optimum waveength can be seected for each anayzed substance. Integrated Peak Detector The integrated Peak Detector works as a basic fraction coector. The peak detection eve can be freey programmed for peak start and peak end to enhance the coection purity. An integrated 24V output for switching a soenoid vave is used for the fraction coection, which is automaticay operated with a seectabe time deay Optiona-Anaog Output The 3335i PDA Detector is avaiabe with an optiona 4-Channe anaog output. This D/A converter output option is offered to keep the system fexibe to be used with any data acquisition software avaiabe. Technica Specifications* Wetted Materias: Baseine Noise: Baseine Drift: Stainess Stee/PEEK*, Tefon, Gas ± 0.6 x 10-5 AU (@240 nm, 1 sec. Risetime) <3 x 10-4 AU/h Number of Diodes: 256 or 1024 Waveength Range: Waveength Precision: Waveength Accuracy: Temperature Range: Mean Pixe Pitch: nm (256 Diodes) nm (1024 Diodes) ± 0.1nm +0.5 nm (256 Diodes); +0.1 nm (1024 Diodes) C 2.2 nm (256 Diodes), 0.8 nm (1024 Diodes) Resoution ( λ FWHM): 7 nm (256 Diodes), 3 nm (1024 Diodes) > 2.0 AU Light Source Waveength Program: Deuterium Lamp(D2>2000hr ifetime), Tungsten Lamp 10mm Programmabe, 10 steps Anaog Output: - (optiona : 4x 1V) Ce voume: Contro Features: Dimensions: Power Suppy: 1 Hz Hz 10µ Interna Peak Detector with +24 V soenoid switching output. 396 x 165 x 478 mm ~V (47-63 Hz)

11 11 Fuorescence detector 3000 Pus Fuorescence Detector 3000pus Optica System Monochromators Light Source Settabe waveength Measuring waveength Range Spectra bandwidths Waveength Accuracy Waveength Reproducibiity Detectors Ce voume Temperature Operating Range Temperature Accuacy Pressure Sensitivity Sensitivity range Gain Response Power Digita fiter Signa processing Output Input Sef-diagnostics Time programming Spectra scanning Spectra subtraction Lamp timer Lamp off timer Temperature compensation Hoographic concave diffraction grating monochromators for both excitation and emission 150W Xe amp Zero order, nm Both Ex And Em Zero order, nm for both Ex and Em Optiona PM tube extends the range up to 900 nm. Ex: 18 nm Fixed Ex: 18, 40 nm seectabe nm nm Ex: Photodiode Em: photomutipier 10 μi (standard) o 5 C, Ambient temp to 50 C o +0.5 C 2Mpa 500:1(tangent method) 700:1(Baseine Method) 1, 2, 4, 8, 16, 32, 64, 128, 256,and S. x1. x10, x100, x1000 Response FAST, STD, SLOW (0.5, 1.5, 5 Sec.) 3, 5, 10, 20, 40 sec Digita processing by A/D and D/A converters 10mV/FS for recorder (Poarity change is possibe.) 1 V/FS for integrator marker, eak out marker, autozero, program reset/ran memories, DC power suppy, Ex. energy, eak in ce, amp operation time 64 steps, 10 fies, 0.1 to minutes in 0.1 min increments for Ex and Em waveength,range, gain, autozero, spectra bandwidth, and spectra measurement Ex and Em spectra Ex and Em spectra shows accumuated amp operation time settabe from hours (0.1 hour increments) compensates PM tube response for temperature variation o Digita Amperometric EC detector, Mode EC3000 Pus Technica Specification Principe : Amperometric detector with free-eectrode technique. Working Potentia V Storage capacity for Measurement Range + (10pA-20uA) measurement program 0-99 Auto Zero Range max +50uA Storage capacity for ce-ceaning Manua offset Range max +50uA program 0-99 LCD - Dispay dispay of setting and measurement data Anaogram Output + 1V per measurement Fiter 5Hz Hz ( sec) range Detector noise eve <0.3 pa Auto-Zero interface active ow Ceaing Potentia V Input v, 50-60Hz Detay time ceaning sec Output 12 V DC, 205 A Ceaning Cyce every 1st to 10th cyce Dimensions 260 x 251 x 160 mm Weight 7.6 kg

12 12 Product Specification 3359i Refractive Index Detector Detection Method: Micro Anaytica Semi-Preparative Defection Refractive Index Range: 1.00 to 1.75 Fow Rate: m/min m/min 1-50 m/min Ce Voume: 4 µ, 45 ange 10 µ, 45 ange 7 µ, 5 ange Fow Ce Pressure: 6 kg/cm² 6 kg/cm 6 kg/cm Dead Voume: 6 µ 24 µ 88 or 353 µ Linearity Range: µriu µriu µriu Noise Leve: 10 x 10-9 RIU 5 x 10-9 RIU 10 x 10-8 RIU Drift with 1m H 2 O/min Integrator Output: ± 1 V ± 1 V ± 1 V Recorder Output: ± 10 mv/ 100 mv/ 1 V Recorder Offset: 0 mv/ 10 mv/ 100 mv Recorder Range: 8 steps (1:8) - 16:1) Digita Interface: Digita Output: Digita Input: Temperature Setting: Temperature Accuracy: Time Constant: Weight: Dimensions: (W x H x D) Power Suppy: 7 1x10 RIU/hour * A technica specifications may be subject to change. 7 1x10 RIU/hour RS232, Purge, Autozero, Start, Stop, DataOut: 1 Hz, 10 Hz TTL: Intensity Aarm TTL: Purge, Autozero, Start, Marker Ambient, 35 C to 55 C in 1 C steps, Therma Fuse 75 C +0.5 C RAW (0.0 sec.), Fast (0.4 sec.), Medium (0.8 sec.), Sow (1.2 sec.) Evaporative Light Scattering Detector 3000Pus 13 kg 396 x 165 x 478 mm / ~V (50/60 Hz), 50 VA Evaporative Light Scattering Detector 3000pus Light Source Tungsten / Haogen Temperature Range o C, 0.1 o C Increment, feedback accuracy to 0.1 o C Fow Rate 0.1mL/min - 2.5mL/min (standard) Air Consumption <3.0L/min Air Required >5L/min nitrogen gas or air, 65 Psi Nebuizer(high fow rate) μi/min Nebuizer chamber Thermay controed, Heater 0-100%, Cooer(on/off) Gain Setting Sampe Rate upto 80 Points Optics Heated Optics Bench Lamp caibration Pre aigned assemby Detector PMT Scattering ange 60 Degree Measurement range ight scattering units fu scae Ange Output 2, LS units nebuizer,drift tube, CHM Gas pressure Low-Temperature Evaporation ELSD 3000pus is an innovative gain contro avaiabe when it is driver-controed by software, it automaticay adapts the gain setting to avoid any off-scae saturation of the detector. Easy Mantainanence A singe and dedicated HPLC nebuizer and an innovative ce design minimize band broadening. This nebuizer covers the fow rate range from 100µL/min to 3000µL/min and can be readiy and quicky mounted and dismounted. 7 1x10 RIU/hour

13 i Coumn Oven The Anaytica 3411i Coumn Oven is a contact heat transfer oven for high temperature stabiity and accuracy. The coumns are mounted inside the coumn oven in optimized coumn hoder which encose the compete coumn to get the best temperature transfer between the heater and the coumn. Up to two coumns (max. ength 250 mm) can be mounted at the same time (max. O.D. 8 mm). Heating The Anaytica 3411i Coumn Oven standard version features a high temperature controer for stabe coumn temperatures of +30 C up to +100 C. The temperature accuracy is within 0.1 C. Heating/Cooing The Anaytica 3411i Coumn Oven is aso avaiabe with active Heating/Cooing with Petier technique. The temperature range is +5 C up to +100 C. The Heating/Cooing unit uses the same efficient controer as the basic version with temperature accuracy better than 0.1 C. Leakage Sensor The 3441i Coumn Oven offers a high sensitive Leakage Sensor which detects the vapors of organic sovents. OEM Options The Anaytica 3441i Coumn Oven itsef is avaiabe as a compete OEM instrument. Pease contact us for any further information on OEM modues. Temperature Time Program The Heating/Cooing variant offers an optiona Temperature Time Program for stand-aone operation. Integrated Vave The Anaytica 3411i Coumn Oven offers the option to incude an automatic switching vave of the Anaytica 3600, for exampe the 3607 Coumn Seection Vave, but a 3600 can be integrated. Temperature Fuse Besides a Leakage Sensor the 3441i offers a temperature fuse which shuts down the unit when the temperature becomes too high, because of an eectronic defect. Technica Specifications Wetted Materias: Stainess Stee / PEEK 1, PPS 1 Temperature Range: Temperature Accuracy: < 0.1 C Switching Vave: +30 C +100 C (min.: ambient +5 C) optiona : +5 C +100 C (Petier) 2 optiona : any Vave Temperature Program: Safety Features: Dimensions: (W x H x D) Power Suppy: optiona with Heating/Cooing (Petier) Temperature Fuse; Leakage Sensor 396 x 165 x 478 mm ~V (47-63 Hz) 1 Switching Vave: depending on configuration 2 Temperature range at 20 C ambient

14 i Coumn Oven The Anaytica 3412i Coumn Oven is a contact heat transfer oven for high temperature stabiity and accuracy. The coumns are mounted inside the coumn oven in optimized coumn hoder which encose the compete coumn to get the best temperature transfer between the heater and the coumn. Up to three 350mm coumns can be mounted at the same time. Heating The Anaytica 3412i Coumn Oven standard version features a high temperature controer for stabe coumn temperatures of +30 C up to +150 C. The temperature accuracy is within 0.1 C. Leakage Sensor The 3412i Coumn Oven offers a high sensitive Leakage Sensor which detects the vapors of organic sovents. Temperature Fuse Heating/Cooing The Anaytica 3412i Coumn Oven is aso avaiabe with active Heating/Cooing with Petier technique. The temperature range is +5 C up to +100 C. The Heating/Cooing unit uses the same efficient controer as the basic version with temperature accuracy better than 0.1 C. Temperature Time Program The Heating/Cooing variant offers an optiona Temperature Time Program for stand-aone operation. Integrated Veve The Anaytica 3412i Coumn Oven offers the option to incude an automatic switching vave of the Anaytica 3412i, for exampe the 3600 Coumn Seection Vave, but a 3600 Vaves can be integrated. Besides a Leakage Sensor the 3412i offers a temperature fuse which shuts down the unit when the temperature becomes too high, because of an eectronic defect. OEM Options The Anaytica 3412i Coumn Oven itsef is avaiabe as a compete OEM instrument. Pease contact us for any further information on OEM modues. Technica Specifications Wetted Materias: Stainess Stee / PEEK 1, PPS 1 Temperature Range: Temperature Accuracy: < 0.1 C Temperature stabiity: Switching Vave: Temperature Program: Safety Features: Dimensions: (W x H x D) Power Suppy: +30 C +150 C (min.: ambient +5 C) optiona: +5 C +100 C (Petier) C to set temperature optiona: any Vave optiona with Heating/Cooing (Petier) Temperature Fuse; Leakage Sensor 183 x 566 x 270 mm ~V (47-63 Hz) 1 Switching Vave: depending on configuration 2 Temperature range at 20 C ambient

15 15 Fraction Coector (3100 Pus) Features The ony fraction coector that can easiy be programmed for any rack or recipients of your choice. A eectronic and mechanica components have been miniaturizer and paced in one moving part, which is paced above the fractions. Therefore, there is no danger of spiing. The ower part of the fraction coector can be paced into a cod bath, ice bath or any other thermostabiizer container. The ower part of the fraction coector can be paced into a cod bath, ice bath or any other thermostabiizer container. Modern microprocessor controed system using severa optica sensors aows for easy programming of the tube positions just with a simpe pen. Fraction coection according to time or voume. Fraction coection in ines or meander ike (zigzag). A pause ( min or min) can be programmed between fractions. Thus, the OMNI- COLL fraction coector can be used for taking (singe or mutipe) sampes, e.g. during fermentation processes, ce cutures and other bioogica or chemica processes. The pause function can be used aso for an automatic start up of the fraction coection. Washing of the tubing (ing washing) between sampes is possibe the number of fractions in a series can be chosen. Auto-stop function switches off the coector and the fractionation after a seected rack or at the end of the ine.. Pump fow stop function avoids spiing between tow consecutive fractions. Ony the iquid transferring tubing is moved instead of the tubes, this requires much ess energy and aows the coector to be miniaturizerd. Additionay, the tubes are easiy accessibe from a sides. The ower part of the coector can be used for carrying of storagge of fractions. A new pastic fixing mat keeps the racks or recipients in position Severa ower parts can be combined to increase the fraction coecting capacity of the coector. Highest user safety has been attained by suppying OMNICOLL with a ow votage pug-in power suppy. This aows aso an easy fied appication of the fraction coector(battery operation possibe). Soid meta construction makes the OMNICOLL fraction coector insensitive to sovents. Can be easiy disassembed and requires ony itte storage space. Low- maintenance construction Competitive price. Remote contro aows samping after reception of an externa signa (such as an aarm). In this way, it is possibe to obtain important sampes during ong processes running even during absence of a supervisor. Drop counter, inert vave, RS-232 interface and other accessories are avaiabe as an option.

16 16 Product Specification Technica Specifications Fractionation: Time min in 0.1 min steps or min in 1 min steps Voume m or Drop counter ( or ) (option) Through externa signa or RS-232 interface (option) With or without a pause from min or min Tube capacity: Power suppy: Safety: Operating temperature: Weight: Dimensions: Guarantee: According to your choice (avaiabe surface of cm) e.g. using Nagene economy racks: 300 tubes 13 mm diameter Tube capacity can be increased severa times (by adding severa ower parts together) 204 tubes 16 mm diameter 130 tubes 20 mm diameter 80 tubes 30 mm diameter 9 VDC/12 W, using pug-in power suppy ( V, Hz) Meets CE and IEC 1010/1 norms for aboratory instruments 0 to 40 o C 6.5 Kg 34x30x49 cm (W x H x D) 2 years Fraction Coector (3100) Features Unique menu-operationa processes. Press Concise Fow Chart on the pane and that is abe to operate easiy and convenienty; Rectanguar design and Hanging-type X-Y moves for accuracy of tube change; Secure use with circuit separation when iquid coecting; Corrosion-resistant design -- appication to coumn chromatography separation experiments for watersoube sovent or organic sovents; Programmabe contro -- microprocessor-controed and the user can design its own coection procedures according to chromatography by the operation pane; Coecting with differant capacities and waste tank -- Free combination of different tube racks avaiabe for SP-16A,SP-36A, SP-100A, SP-169A; achieve a muti-purpose machine functions and matching (SP-36L) can be repeated unimited consecutive coection to the efficiency of preparation; waste fow into the tank automaticay through threeway vave and contro procedures; Peak coecting with spectra show - the coection information showed on the screen to make the peak coection easiy and convenient to the spectrum processing and quantitative anaysis; Coection time deay set avaiabe -- can be set to deay coection time. Programmaticay set the deay time to ensure that the sampe accuratey into the right tube; Tube instead with soenoid vave contro -- Through the soenoid vave contro to prevent iquid eakage. Technica Specifications Dispay: Tubes: Timing range: Drop range: Pack range: Programmabe range: Moves: Operation: Procedures avaiabe: Data retention with power outages: Tube rack: Power Suppy: Use of the environment: Weight: Dimensions: Bue LCD dispay 100 (each max capacity: 12m with a waste tank) 1 s-999 h 59 m 59 s (arbitrary) 1 drop drop (arbitrary) 1-200mv (arbitrary) 1-10sect (arbitrary) Hanging X-Y Tube instead with 12V DC soenoid vave Chinese menu - stye 1-10 modes Ten years rectanguar design with a waste tank; free combination; optiona (SP- 169A, 36A,16A are optiona and scaabe avaiabe for 36L) AC220V+ 10%; 50Hz; 10w Temperature 0-40 o C; Humidity < 85% 10Kg 355x360x270mm

17 i Vacuum Degasser The Anaytica 3751i Vacuum Degasser is an onine degasser system with high efficiency. Dissoved gases are removed from the sovents by appying vacuum to a semi-permeabe membrane. Working Principe The sovent fows through a short ength of Tefon AF capiary inside a seaed chamber. This chamber (vacuum chamber) is competey seaed to the environ - ment and vacuum is appied with a pump. Due to this vacuum any dissoved gases in the sovent running through the inner capiary are removed through its semi-permeabe membrane wa. The high efficiency of the Tefon AF materia aows the usage of a very short ength of capiary inside the vacuum chamber. Wetted Materias: Degassing Capacity: Voume / Channe: < 500µ Dimensions: (W x H x D) Weight: Power Suppy: Tefon AF, Tefon, Stainess Stee, Auminium, EPDM < 20% dissoved gases remaining in water at 1.0 m/min 125 x 167 x 270 mm 3.2 kg ~V (47-63 Hz) High Efficiency The high efficient Tefon-AF capiary has a much higher efficiency than a norma Tefon capiary of simiar size. This aows the usage of a smaer ength of capiary to reduce the dead voume of the system consideraby. 2 Operation Modes The 3751i can be run either with constant speed or in Hysteresis Mode, which switches the vacuum pump on or off. 5-Year Membrane Warranty The 3751i vacuum pump uses a membrane for creating the vacuum. This membrane is made of a specific Tefon materia specificay designed for fast movements. Anaytica offers a 5-year warranty on the ifetime of this membrane. Muti-Channe The 3751i Vacuum Degasser is avaiabe as 1-Channe, 2-Channe, 3-Channe, or 4-Channe version. Each sovent channe can be used for a different sovent. Severa channes can be used in series to increase the efficiency even more. OEM Options Anaytica offers this instrument as an OEM product to any interested party, even for sma numbers. Besides the compete instrument Anaytica aso offers the vacuum pump and vacuum chambers as OEM modues.

18 18 DAC Coumns DAC stands for dynamic axia compression. It combines the preparative coumn and packing system together. It is very simpe to operate. The coumn can be used onine when it is packed we.don t need to take the coumn down. It prevents mechanica degradation of the partices. Bed compression is maintaned constant, independent of sweing or shrinking of the bed. If the sovent conditions are such that partice sweing takes pace, then the piston automaticay et the bed expand to maintain constant compression. Anaytica-PACK DAC coumns can be consistenty packed with sma particuate media (10um and ess) to high eves of performance: coumn efficiencies of up to 50,000 theoretica pates per meter have been reported for coumns of 50 and 300 mm interna diameter(i.d). This requires and even distribution of the iquid fow over the coumn cross-section. Our engineers have modeed the fow pattern at the coumn ends and designed efficient fow distribution system for optimum performance. For even better contro of chromatographic conditions, DAC coumn are deivered with a temperature jacket and/or insuation. Technica Specification Coumn: Interna diameter: ID30mm-ID1000mm Hydrauic cyinder: Maximnm working pressure: 50MPa Tube enght:650mm Viscosity range: 46-68cst. Tube interna suffice finish: Ra -<0.8m Pressure Indicator Air pressure meter:0-10bar, 0.01 degree Materia:SST316 Sintered Hydrauic meter:0-400bar, 0.1 degree Fiter: Materia: SST316 sintered Working pressure 120bar max. piston act on the coumn pore size: 3-5um bed Piston: Materia:SST316L Air suppy requirement: Rated air out pressure 0.5-1Mpa Anaytica Semi-preparation/Preparative HPLC Coumn Anaytica HPLC Coumn Dimension: ID 4.6mm, Length mm Connection:1/16 (Standard) Packing Materia: Word famous brand materia, such as Kromasi, Luna, YMC, Daiso etc. Semi-Preparative HPLC Coumn Dimension: ID 10-40mm, Length mm Connection:1/16 (Standard) Packing Materia: Word famous brand materia, such as Kromasi, Luna, YMC Daiso etc. Preparative HPLC Coumn Dimension: ID mm, Length mm Connection: 1/8 or 1/14 (Standard) Packing Materia: Word famous brand materia, such as Kromasi, Luna, YMC Daiso etc. We can produce a kinds of hpc coumns basis of customers requirement. Preparative hpc coumn re-packing service Preparative hpc counm appication support. We promise that each counm must be tested stricty and attached with test report. HSCCC HSCCC has unique advantages in the isoation and purification work of natura products and antibiotics.

19 19 ANALCHROM TM Chromatography Workstation Software Anachrom Chromatography workstation software data handing system is compatibe with any mode of chromatographic anaytica instrument avaiabe in the market. Our state of the art hardware comes in two different modes, whie you can connect the two channes to the dua detectors of the same instrument for simutaneous data acquisition, you can aso connect them to the two different instruments for independent data acquisition. This software system is structured in such a way that a the sequences reated to an anaysis (Standard from acquisition of raw data signa, to integration of chromatogram, to cacuation of components quantities, through to preparation of anaysis report) are incorporated in one serve document caed chromatogram fie. By appying document windows technique and spit window technique, we have design a one page document window for you to dispay the entire working eement for chromatogram fie in one screen for quick access and manipuation. Anachrom is iquid chromatography software package deveoped by Anaytica Technoogies. It provides compete contro and data processing function with simpe and efficient operation. Compies with 21 CFT/GLP/GMP features. PDA/DAD (Diode Array Detector) contro & acquisition is possibe. Onine GPC (Ge Permeation Chromatography) cacuation. Licensed HPLC software for contro of a HPLC modues ike pump, tector etc. for quaitative and quantitative anaysis. Parameter vaidation management. Compatibiity with genera detectors. Compatibiity to import and export the data. Abiity to acquire and anaysis and store spectra ibrary. Fexibe report pubisher to make report in desired form. Shoud have system suitabiity option. ANALCHROM is the most universa software of the entire product ine. GLP Compiance. A other versions are derives from the fu carity software. INTEGRATION There is extensive possibiity to modify chromatograms. The chromatogram can be changed by entering by goba parameters or interactivey, through direct graphic modification of the base ine. OVERLAY Simutaneousy dispay a virtuay unimited number of chromatograms and their mathematica modification; for exampe, mutua deductions or derivations of any order. CALIBRATION Interna and externa standard cacuation methods, caibration of groups of peaks and reference peaks method for better identification. USER SETTINGS User seects parameter for peak dispay and the specification for axes, incuding coor from and extensive array of coor settings. Text abes and ines, either as part of the area or anchored to a chromatogram, may aso be inserted. METHOD AND CALIBRATION HISTORY Each Chromatogram can easiy be dispayed under the same conditions as when it was printed, exported or saved. COLUMN PERFORMANCE Cacuation of peaks in terms of symmetry, efficiency, resoution; a by severa methods (Tangent, moments, etc) BATCH Automaticay batch processes, dispays, exports or prints any number of chromatogram. USER ACCOUNTS Set up access rights and passwords (Incuding their parameters e.g. minimum ength, vaidity, etc) each user can define his or her own station appearance. AUDIT TRAIL Records seected events and operations into a specia fie. Records seected operations directy into chromatogram. ELECTRONIC SIGNATURE Each Chromatogram can be signed eectronicay. Signature seection is based on the signature certificate.

20 20 Spares & Consumabes / Coumns / Source Lamps / Pre-Instaation Requirements Chemicas, Reagents & Medica Diagnostic kit HPLC Consumabes ike Check Vaves Punger, punger Seas Coumns (Anaytica And Prep), puse Damper, Mixer PEEK Nuts, SS Mae, Nuts, Sovent Reservoir Fiter, Septas, Vias, Rotor Sea, Stator Face Assy, Loops, Syringes, Bearings, O-Rings, Line Fiters for any make of HPLC system. Fow Spitters, Puse dampers, Muti Purpose HPLC Pumps (Isocratic & Gradient) & Manua Injectors CHIRAL, Preparative, Anaytica Capiary HPLC Coumns and guard coumns, GPC Coumns, Combi Chem and Amino Acid, Microbore HPLC Coumns, BULK Materias for Coumns, frits and Hardware for Coumns. Source Lamps for a Anaytica Instruments such as D2, Haogen, Xenon, Tungsten, mercury vapors haow cathode amps etc. Hematoogy Reagent Bio Chemistry Reagents Urine Strips Medica Diagnostic kit We offer a the Consumabes / Reagents Quaity / Contro Standards / Caibration Standards / Vaidation Standards required for running the anayzers ike HPLC, SPECTROPHOTOMETER, FTIR, HEMATOLOGY ANALYZER, BIO- CHEMISTRY ANALYZERS (Cinica Chemistry Anayzers). We aso manfacture reagents for Hematoogy and Bio Chemistry, Urine Anaysis.

21 21 Support & Services HPLC Servicing, Vaidation, Trainings and Preventive Maintenance : HPLC Servicing :We have team of service engineers who can attend to any make of HPLC most affordabe cost. Trainings :We aso take up preventive Maintenace to reduce downtime of HPLC s AMC s/cmc :We offer user training both in-house and at customer sites on HPLC principes, operations, troubeshooting. Vaidations :We have protocos for carrying out periodic Vaidations as per GLP/GMP/U SFDA norms Instruments :We offer instruments / Renting Services Modues ike pumps, detector etc. on Rent. About Anaytica Technoogies Anaytica Technoogies is synonymous for offering technoogies for doing anaysis and is the Fastest Growing Goba Brand having presence in at east 96 countries across the gobe. Anaytica Technoogies Limited is an ISO :9001 Certified Company engaged in Designing, Manufaturing, Marketing & providing Services for the Anaytica, Chromatography, Spectroscopy, Bio Technoogy, Bio Medica, Cinica Diagnostics, Materia Science & Genera Laboratory Instrumentation. Anaytica Technoogies, India has across the Country operations with at east 4 Regiona Offices, 6 Branch Offices & Service Centers. Distributors & Channe partners wordwide. About Anaytica Technoogies UV/VIS Spectro Doube Beam PTIR Gas Chromatograph 3000 Gas Chromatograph 2979 Pus Fash Chromatograph Atomic Absorption Spectrophotometer Liquid Partica Counter Optica Emission Spectrophotometer DSC/TGA NOVA 2020 pus Automated Bio Chemistry Anayzer HEMA 2020 Hematoogy Anayzer Micro Pate Reader/Washer Water purification system Tota Organic Carbon Fuy Automated CLIA NOVA Basic Semi -Auto Chemistry Anayzer PCR/Gradient PCR/ RTPCR Bood Gas Anayzer Random access Anayzer for immunoassay Proteins & cinica chemistry Semen Anayzer

22 Reguatory compiances Corporate Socia Responsibiity Reach Corporate & Regd. Office: Anaytica House, # E67 & E68, Ravi Park, Vasna Road, Baroda, Gujarat INDIA Anaytica Foundation is a nonprofit organizat (NGO) found for the purpose of: 1.Research & Innovation Scientist s awards / QC Professiona Award : Quaity ife is possibe by innovation ony and the innovation is possibe by research ony, hence ANALYT- ICAL FOUNDATION is committed to identify such personaities for their contributions across various fied of Science and Technoigy and awarding them yeary. To participate for award, send us your detais of research / testing / pubication at Info@anayticafoundation.org 2. Improving quaity of ife by offering YOGA Training courses, Work shops / Seminars etc. 3. ANALYTICAL FOUNDATION aims to DETOXIFY human minds, sous and boby by means of yoga, Meditation, Ayurveda, Heath Care, Awards, Media, Events, Comps etc. HPLC Soutions MutipeLabs Anaytica Bio-Med Anaytica Distributors Anaytica Foundation (Trust) T: F: E: info@hpctechnoogies.com info@mutipeabs.com info@anayticagroup.net info@anayticabiomed.com W Saes & Support Offices: across the country : Distributors & Channe partners Word Wide Note : Company reserves rights to add/deete/modify the contents / technica specificationsof the cataogue without prior notice.